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List of works by David D Thomas

(1)H/(15)N heteronuclear NMR spectroscopy shows four dynamic domains for phospholamban reconstituted in dodecylphosphocholine micelles.

scientific article

2-Color Calcium Pump Reveals Closure of the Cytoplasmic Headpiece with Calcium Binding ⬇️

scientific article published on July 11, 2012

A Cardiomyopathy Mutation in the Myosin Essential Light Chain Alters Actomyosin Structure

scientific article published in July 2017

A Novel Fluorescence Resonance Energy Transfer-Based Screen in High-Throughput Format To Identify Inhibitors of Malarial and Human Glucose Transporters

scientific article

A bifunctional spin label reports the structural topology of phospholamban in magnetically-aligned bicelles.

scientific article

A continuous fluorescence displacement assay for BioA: an enzyme involved in biotin biosynthesis.

scientific article

A human β-III-spectrin spinocerebellar ataxia type 5 mutation causes high-affinity F-actin binding.

scientific article published on 17 February 2016

A novel SERCA inhibitor demonstrates synergy with classic SERCA inhibitors and targets multidrug-resistant AML.

scientific article published on 30 September 2013

A posttranslational modification of the mitotic kinesin Eg5 that enhances its mechanochemical coupling and alters its mitotic function

scientific article published on 5 February 2018

ATP-Binding Cassette Transporter Structure Changes Detected by Intramolecular Fluorescence Energy Transfer for High-Throughput Screening.

scientific article published on 29 April 2015

Accurate quantitation of phospholamban phosphorylation by immunoblot.

scientific article published on 3 February 2012

Actin filament dynamics in the actomyosin VI complex is regulated allosterically by calcium-calmodulin light chain

scientific article published on 6 September 2011

Actin-Myosin Interaction: Structure, Function and Drug Discovery

scholarly article by Piyali Guhathakurta published in September 2018

Actin-binding compounds, previously discovered by FRET-based high-throughput screening, differentially affect skeletal and cardiac muscle

scientific article published on 11 August 2020

Age affects myosin relaxation states in skeletal muscle fibers of female but not male mice

scientific article published in PLoS ONE

Age-related decline in actomyosin structure and function.

scientific article

Allosteric communication in Dictyostelium myosin II ⬇️

scientific article published on July 1, 2012

Allostery governs Cdk2 activation and differential recognition of CDK inhibitors

scientific article published on 01 February 2021

Amplitude of the actomyosin power stroke depends strongly on the isoform of the myosin essential light chain

scientific article

An Innovative High-Throughput Screening Approach for Discovery of Small Molecules That Inhibit TNF Receptors

scientific article published on 22 May 2017

An autoinhibitory peptide from the erythrocyte Ca-ATPase aggregates and inhibits both muscle Ca-ATPase isoforms

scientific article

Aqueous sample in an EPR cavity: sensitivity considerations.

scientific article published on March 2004

Atomic-level mechanisms for phospholamban regulation of the calcium pump.

scientific article

Bifunctional Spin Labeling of Muscle Proteins: Accurate Rotational Dynamics, Orientation, and Distance by EPR.

scientific article

Calcium Stimulates Self-Assembly of Protein Kinase C α In Vitro

scientific article

Calcium-Dependent Structural Dynamics of a Spin-Labeled RyR Peptide Bound to Calmodulin.

scientific article published in December 2016

Calmodulin oxidation and methionine to glutamine substitutions reveal methionine residues critical for functional interaction with ryanodine receptor-1.

scientific article published on 13 February 2003

Cardiac myosin binding protein-C restricts intrafilament torsional dynamics of actin in a phosphorylation-dependent manner.

scientific article

Changes in Band 3 oligomeric state precede cell membrane phospholipid loss during blood bank storage of red blood cells

scientific article

Characterization of a myosin VII MyTH/FERM domain.

scientific article

Cofilin increases the torsional flexibility and dynamics of actin filaments

scientific article published on 26 September 2005

Conformationally trapping the actin-binding cleft of myosin with a bifunctional spin label

scientific article published on 18 December 2012

Controlling the inhibition of the sarcoplasmic Ca2+-ATPase by tuning phospholamban structural dynamics.

scientific article published on 30 September 2007

Cysteine reactivity and oligomeric structures of phospholamban and its mutants.

scientific article published on September 1998

Defining the molecular components of calcium transport regulation in a reconstituted membrane system

scientific article published on 01 April 2003

Depolymerization of phospholamban in the presence of calcium pump: a fluorescence energy transfer study

scientific article published on 01 March 1999

Differences in structural dynamics of muscle and yeast actin accompany differences in functional interactions with myosin

scientific article published on 01 November 1999

Different anesthetic sensitivities of skeletal and cardiac isoforms of the Ca-ATPase.

scientific article published in July 1999

Direct detection of SERCA calcium transport and small-molecule inhibition in giant unilamellar vesicles.

scientific article

Direct detection of phospholamban and sarcoplasmic reticulum Ca-ATPase interaction in membranes using fluorescence resonance energy transfer.

scientific article

Direct real-time detection of the actin-activated power stroke within the myosin catalytic domain

scientific article published on 15 April 2013

Direct real-time detection of the structural and biochemical events in the myosin power stroke

scientific article

Discovery of enzyme modulators via high-throughput time-resolved FRET in living cells.

scientific article published on February 2014

Dual mechanisms of sHA 14-1 in inducing cell death through endoplasmic reticulum and mitochondria.

scientific article

Dynamics of Dystrophin's Actin-Binding Domain

scientific article published on 20 June 2018

Dystrophin and utrophin have distinct effects on the structural dynamics of actin

scholarly article

Effect of Phosphorylation on Interactions between Transmembrane Domains of SERCA and Phospholamban.

scientific article

Effects of Ser16 phosphorylation on the allosteric transitions of phospholamban/Ca(2+)-ATPase complex

scientific article published on 07 March 2006

Effects of pseudophosphorylation mutants on the structural dynamics of smooth muscle myosin regulatory light chain.

scientific article published on October 2014

Effects of the Arg9Cys and Arg25Cys mutations on phospholamban's conformational equilibrium in membrane bilayers.

scientific article published on 2 March 2018

Electron paramagnetic resonance resolves effects of oxidative stress on muscle proteins

scientific article published on January 2014

Electron paramagnetic resonance reveals a large-scale conformational change in the cytoplasmic domain of phospholamban upon binding to the sarcoplasmic reticulum Ca-ATPase.

scientific article

Electron paramagnetic resonance: a high-resolution tool for muscle physiology.

scientific article

Endoplasmic reticulum protein BI-1 regulates Ca²⁺-mediated bioenergetics to promote autophagy

scientific article

Erratum: Structural Mechanism for Regulation of Bcl-2 protein Noxa by phosphorylation

scientific article published on 29 October 2015

FRET-based mapping of calmodulin bound to the RyR1 Ca2+ release channel

scientific article

FRET-based trilateration of probes bound within functional ryanodine receptors.

scientific article

Fluorescence lifetime plate reader: resolution and precision meet high-throughput

scientific article

Functional and physical competition between phospholamban and its mutants provides insight into the molecular mechanism of gene therapy for heart failure.

scientific article published on 12 April 2011

Functional and transcriptomic insights into pathogenesis of R9C phospholamban mutation using human induced pluripotent stem cell-derived cardiomyocytes

scientific article published on 09 May 2018

Generalized limits to the number of light particle degrees of freedom from big bang nucleosynthesis

scholarly article by Keith Olive & David D Thomas published September 1999 in Astroparticle Physics

Heart failure drug changes the mechanoenzymology of the cardiac myosin powerstroke.

scientific article published on 21 February 2017

High-Throughput Screens to Discover Small-Molecule Modulators of Ryanodine Receptor Calcium Release Channels

scientific article published on 22 October 2016

High-Throughput Spectral and Lifetime-Based FRET Screening in Living Cells to Identify Small-Molecule Effectors of SERCA

scientific article published on 13 December 2016

High-performance time-resolved fluorescence by direct waveform recording.

scientific article

High-resolution helix orientation in actin-bound myosin determined with a bifunctional spin label.

scientific article published on 8 June 2015

High-throughput FRET assay yields allosteric SERCA activators

scientific article

High-throughput screen, using time-resolved FRET, yields actin-binding compounds that modulate actin-myosin structure and function

scientific article published on 04 June 2018

Impact of methionine oxidation on calmodulin structural dynamics.

scientific article published on 2 December 2014

Impacts of dystrophin and utrophin domains on actin structural dynamics: implications for therapeutic design.

scientific article

Impaired muscle relaxation and mitochondrial fission associated with genetic ablation of cytoplasmic actin isoforms.

scientific article published on 18 December 2017

Insulin-dependent rescue from cardiogenic shock is not mediated by phospholamban phosphorylation

scientific article published on April 2009

Interdomain fluorescence resonance energy transfer in SERCA probed by cyan-fluorescent protein fused to the actuator domain.

scientific article

Interprotein electron transfer in a confined space: uncoupling protein dynamics from electron transfer by sol-gel encapsulation.

scientific article published on August 2002

John Gergely (1919-2013): a pillar in the muscle protein field.

scientific article

Large-scale opening of utrophin's tandem calponin homology (CH) domains upon actin binding by an induced-fit mechanism

scientific article

Lipid-mediated folding/unfolding of phospholamban as a regulatory mechanism for the sarcoplasmic reticulum Ca2+-ATPase

scientific article

Live-Cell Cardiac-Specific High-Throughput Screening Platform for Drug-Like Molecules that Enhance Ca2+ Transport

scientific article published on 08 May 2020

Loop L5 assumes three distinct orientations during the ATPase cycle of the mitotic kinesin Eg5: a transient and time-resolved fluorescence study.

scientific article published on 21 October 2013

Magnesium impacts myosin V motor activity by altering key conformational changes in the mechanochemical cycle.

scientific article

Mapping the interaction surface of a membrane protein: unveiling the conformational switch of phospholamban in calcium pump regulation.

scientific article

Mapping the ryanodine receptor FK506-binding protein subunit using fluorescence resonance energy transfer

scientific article

Measurement of myocardial free radical production during exercise using EPR spectroscopy.

scientific article published on 13 January 2006

Methane monooxygenase hydroxylase and B component interactions.

scientific article published in March 2006

Microsecond molecular dynamics simulations of Mg²⁺- and K⁺-bound E1 intermediate states of the calcium pump

scientific article

Microsecond rotational dynamics of spin-labeled myosin regulatory light chain induced by relaxation and contraction of scallop muscle

scientific article published on 01 October 1998

Molecular Modeling of Fluorescent SERCA Biosensors.

scientific article published on January 2016

Molecular and cellular contractile dysfunction of dystrophic muscle from young mice

scientific article published on 01 July 2006

Molecular dynamics simulations reveal a disorder-to-order transition on phosphorylation of smooth muscle myosin.

scientific article

Multi-state recognition pathway of the intrinsically disordered protein kinase inhibitor by protein kinase A

scientific article published on 27 April 2020

Multibore sample cell increases EPR sensitivity for aqueous samples

scientific article published on 10 November 2005

Muscle activity and aging affect myosin structural distribution and force generation in rat fibers.

scientific article published on 26 September 2003

Muscle chemistry and force.

scientific article published in September 2000

Mutation that causes hypertrophic cardiomyopathy increases force production in human β-cardiac myosin

scientific article published on 12 July 2013

Myosin Orientation in a Muscle Fiber Determined with High Angular Resolution Using Bifunctional Spin Labels

Myosin lever arm orientation in muscle determined with high angular resolution using bifunctional spin labels

scientific article published on 21 June 2019

Myosin light-chain domain rotates upon muscle activation but not ATP hydrolysis

scientific article published on 01 September 1999

NMR solution structure and topological orientation of monomeric phospholamban in dodecylphosphocholine micelles

scientific article

Nucleotide Activation of the Ca-ATPase ⬇️

scientific article published on September 13, 2012

Oligomeric Interactions of Sarcolipin and the Ca-ATPase ⬇️

scientific article published on July 7, 2011

On the function of pentameric phospholamban: ion channel or storage form?

scientific article published on May 2009

Open and closed conformations of the isolated transmembrane domain of death receptor 5 support a new model of activation

scientific article published on March 2014

Optimization of bicelle lipid composition and temperature for EPR spectroscopy of aligned membranes

scientific article

Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies.

scientific article

Oxidation increases the strength of the methionine-aromatic interaction

scientific article published on 22 August 2016

Oxidation of ryanodine receptor (RyR) and calmodulin enhance Ca release and pathologically alter, RyR structure and calmodulin affinity

scientific article

Phospholamban mutants compete with wild type for SERCA binding in living cells.

scientific article

Phospholamban pentamer quaternary conformation determined by in-gel fluorescence anisotropy.

scientific article

Phospholamban phosphorylation, mutation, and structural dynamics: a biophysical approach to understanding and treating cardiomyopathy.

scientific article

Phosphomimetic S3D cofilin binds but only weakly severs actin filaments.

scientific article

Phosphorylated phospholamban stabilizes a compact conformation of the cardiac calcium-ATPase

scientific article published on October 2013

Phosphorylation-dependent conformational switch in spin-labeled phospholamban bound to SERCA.

scientific article published on 9 March 2006

Phosphorylation-induced structural changes in smooth muscle myosin regulatory light chain

scientific article

Protein structural dynamics revealed by site-directed spin labeling and multifrequency EPR.

scientific article published on May 2010

Protein-protein interactions in calcium transport regulation probed by saturation transfer electron paramagnetic resonance.

scientific article

Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening

scientific article published on 24 October 2018

Redox-sensitive residue in the actin-binding interface of myosin

scientific article

Role of calmodulin methionine residues in mediating productive association with cardiac ryanodine receptors.

scientific article

Role of cysteine residues in structural stability and function of a transmembrane helix bundle.

scientific article

Rotational dynamics of phospholamban determined by multifrequency electron paramagnetic resonance.

scientific article

Rotational dynamics of the regulatory light chain in scallop muscle detected by time-resolved phosphorescence anisotropy

scientific article published on 01 July 1999

RyR1-targeted drug discovery pipeline integrating FRET-based high-throughput screening and human myofiber dynamic Ca2+ assays

scientific article published on 04 February 2020

S100A1 Protein Does Not Compete with Calmodulin for Ryanodine Receptor Binding but Structurally Alters the Ryanodine Receptor·Calmodulin Complex

scientific article published on 19 May 2016

Sarcolipin Exhibits Abundant RNA Transcription and Minimal Protein Expression in Horse Gluteal Muscle

scientific article published on 13 November 2020

Sarcolipin Promotes Uncoupling of the SERCA Ca2+ Pump by Inducing a Structural Rearrangement in the Energy-Transduction Domain

scientific article published on 12 October 2016

Sarcolipin and phospholamban inhibit the calcium pump by populating a similar metal ion-free intermediate state.

scientific article published on 15 May 2015

Sequential myosin phosphorylation activates tarantula thick filament via a disorder-order transition.

scientific article published in July 2015

Site-directed spectroscopic probes of actomyosin structural dynamics.

scientific article published on January 2009

Site-directed spectroscopy of cardiac myosin-binding protein C reveals effects of phosphorylation on protein structural dynamics.

scientific article published on 23 February 2016

Site-directed spin labeling reveals a conformational switch in the phosphorylation domain of smooth muscle myosin

scientific article

Site-specific mutations in the myosin binding sites of actin affect structural transitions that control myosin binding.

scientific article published in November 2001

Solid-state NMR and rigid body molecular dynamics to determine domain orientations of monomeric phospholamban

scientific article published on 01 August 2002

Soluble Extracellular Domain of Death Receptor 5 Inhibits TRAIL-Induced Apoptosis by Disrupting Receptor-Receptor Interactions.

scientific article

Spectral Unmixing Plate Reader: High-Throughput, High-Precision FRET Assays in Living Cells

scientific article published on 23 November 2016

Spectroscopic validation of the pentameric structure of phospholamban

scholarly article

Structural Impact of Phosphorylation and Dielectric Constant Variation on Synaptotagmin's IDR.

scientific article published in February 2018

Structural Mechanism for Regulation of Bcl-2 protein Noxa by phosphorylation.

scientific article published on 28 September 2015

Structural and functional dynamics of an integral membrane protein complex modulated by lipid headgroup charge

scientific article published on 28 February 2012

Structural and functional impact of site-directed methionine oxidation in myosin.

scientific article published on 8 November 2011

Structural basis for high-affinity actin binding revealed by a β-III-spectrin SCA5 missense mutation

scientific article published on 7 November 2017

Structural dynamics of actin during active interaction with myosin: different effects of weakly and strongly bound myosin heads.

scientific article

Structural dynamics of muscle protein phosphorylation.

scientific article published on 29 August 2012

Structural dynamics of the actomyosin complex probed by a bifunctional spin label that cross-links SH1 and SH2.

scientific article published on 19 September 2008

Structural dynamics of the myosin relay helix by time-resolved EPR and FRET.

scientific article

Structural kinetics of myosin by transient time-resolved FRET

scientific article

Structural mapping of divergent regions in the type 1 ryanodine receptor using fluorescence resonance energy transfer

scientific article

Structure and dynamics of the force-generating domain of myosin probed by multifrequency electron paramagnetic resonance.

scientific article published on 13 March 2008

Structure and function of integral membrane protein domains resolved by peptide-amphiphiles: application to phospholamban

scientific article published on 01 July 2003

Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes

scientific article published on 01 January 2007

Synthetic null-cysteine phospholamban analogue and the corresponding transmembrane domain inhibit the Ca-ATPase

scientific article published on 01 September 2000

Synthetic phosphopeptides enable quantitation of the content and function of the four phosphorylation states of phospholamban in cardiac muscle

scientific article

Talin influences the dynamics of the myosin VII-membrane interaction.

scientific article

Tarantula myosin free head regulatory light chain phosphorylation stiffens N-terminal extension, releasing it and blocking its docking back

scientific article published in July 2015

Targeting protein-protein interactions for therapeutic discovery via FRET-based high-throughput screening in living cells

scientific article published in Scientific Reports

The Carboxy-Terminal Third of Dystrophin Enhances Actin Binding Activity ⬇️

scientific article published on December 28, 2011

The alpha-helical propensity of the cytoplasmic domain of phospholamban: a molecular dynamics simulation of the effect of phosphorylation and mutation.

scientific article

The myosin super-relaxed state is disrupted by estradiol deficiency.

scientific article

The role of sarcolipin and ATP in the transport of phosphate ion into the sarcoplasmic reticulum.

scientific article

The structural kinetics of switch-1 and the neck linker explain the functions of kinesin-1 and Eg5.

scientific article

Thermodynamic and structural basis of phosphorylation-induced disorder-to-order transition in the regulatory light chain of smooth muscle myosin.

scientific article published on 21 August 2008

Three distinct actin-attached structural states of myosin in muscle fibers.

scientific article published on 6 March 2012

Time-resolved FRET reveals the structural mechanism of SERCA-PLB regulation

scientific article published on 9 May 2014

Transient kinetics and mechanics of myosin's force-generating rotation in muscle: resolution of millisecond rotational transitions in the spin-labeled myosin light-chain domain.

scientific article

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces death receptor 5 networks that are highly organized.

scientific article published on 10 April 2012

β-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization

scientific article published on 16 October 2017

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