List of works - Ryota Iino

A Microfluidic Channel Method for Rapid Drug-Susceptibility Testing of Pseudomonas aeruginosa

scientific article

A microfluidic device for simple and rapid evaluation of multidrug efflux pump inhibitors

scientific article published on 8 February 2012

A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate

scientific article published on July 2015

A single-molecule enzymatic assay in a directly accessible femtoliter droplet array ⬇️

scientific article published on October 28, 2010

Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization.

scientific article published in July 2003

Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array

scientific article published on 21 August 2019

Activation and stiffness of the inhibited states of F1-ATPase probed by single-molecule manipulation.

scientific article

Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase

scientific article published on 2 October 2013

Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force.

scientific article published in January 2013

Correction: Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis

scientific article

Correlation between the conformational states of F1-ATPase as determined from its crystal structure and single-molecule rotation

scholarly article

Design of a large-scale femtoliter droplet array for single-cell analysis of drug-tolerant and drug-resistant bacteria

scientific article published on 4 October 2013

Direct observation of intermediate states during the stepping motion of kinesin-1.

scientific article published on 29 February 2016

Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases

scientific article published on 18 August 2020

Dynamic structural states of ClpB involved in its disaggregation function.

scientific article published in June 2018

Evaluation of multidrug efflux pump inhibitors by a new method using microfluidic channels

scientific article

F1-ATPase: a highly coupled reversible rotary motor

scientific article published on 01 November 2006

Fluctuation theorem applied to F1-ATPase.

scientific article published on 28 May 2010

High-speed angle-resolved imaging of a single gold nanorod with microsecond temporal resolution and one-degree angle precision.

scientific article

High-speed atomic force microscope combined with single-molecule fluorescence microscope.

scientific article

High-speed atomic force microscopy reveals rotary catalysis of rotorless F₁-ATPase.

scientific article

High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies

scientific article published on 16 March 2019

Highly sensitive restriction enzyme assay and analysis: a review.

scientific article published on 22 April 2008

Intersubunit coordination and cooperativity in ring-shaped NTPases.

scientific article published on 08 February 2013

Introduction: Molecular Motors

scientific article published on 01 January 2020

Key chemical factors of arginine finger catalysis of F1-ATPase clarified by an unnatural amino acid mutation.

scientific article

Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy.

scientific article

Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria.

scientific article published in January 2018

Large-scale femtoliter droplet array for digital counting of single biomolecules.

scientific article published on December 2012

Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single-molecule-fluorescence video imaging.

scientific article published in June 2003

Mechanism of inhibition by C-terminal alpha-helices of the epsilon subunit of Escherichia coli FoF1-ATP synthase.

scientific article published on May 2009

Molecular structure and rotary dynamics of Enterococcus hirae V₁-ATPase.

scientific article

Motion capture and manipulation of a single synthetic molecular rotor by optical microscopy.

scientific article published on 2 July 2014

Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy

scientific article published in Scientific Reports

Operation mechanism of F(o) F(1)-adenosine triphosphate synthase revealed by its structure and dynamics

scientific article

Phosphate release in F1-ATPase catalytic cycle follows ADP release.

scientific article published on 26 September 2010

Plasmid-Based One-Pot Saturation Mutagenesis and Robot-Based Automated Screening for Protein Engineering.

scientific article

Principal role of the arginine finger in rotary catalysis of F1-ATPase.

scientific article published on 8 March 2012

Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin

scientific article published in Nature Communications

Publisher Correction: Dynamic structural states of ClpB involved in its disaggregation function

scientific article published on 12 July 2019

Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis.

scientific article published in November 2017

Real-time fluorescence visualization of slow tautomerization of single free-base phthalocyanines under ambient conditions.

scientific article

Rotation and structure of FoF1-ATP synthase.

scientific article

Rotation of artificial rotor axles in rotary molecular motors.

scientific article published on 19 September 2016

Rotational mechanism of Enterococcus hirae V1-ATPase by crystal-structure and single-molecule analyses

scientific article published on 19 March 2015

Simple dark-field microscopy with nanometer spatial precision and microsecond temporal resolution.

scientific article published on May 2010

Simultaneous Observation of Kinesin-Driven Microtubule Motility and Binding of Adenosine Triphosphate Using Linear Zero-Mode Waveguides

scientific article published on 03 December 2018

Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface.

scientific article published on June 2001

Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array

scientific article published on January 2016

Single-Nanoparticle Tracking with Angstrom Localization Precision and Microsecond Time Resolution

scientific article published on 17 November 2018

Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose.

scientific article published on 8 September 2016

Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme of Enterococcus hirae V1-ATPase

scientific article published on 13 September 2019

Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII

scientific article

Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens

scientific article published on 10 January 2020

Single-molecule imaging and manipulation of biomolecular machines and systems.

scientific article published on 5 August 2017

Small stepping motion of processive dynein revealed by load-free high-speed single-particle tracking

scientific article published on 23 January 2020

Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus

scientific article

Temperature-sensitive reaction intermediate of F1-ATPase.

scientific article

The fence and picket structure of the plasma membrane of live cells as revealed by single molecule techniques (Review).

scientific article

Torque generation of Enterococcus hirae V-ATPase

scientific article

Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques

scientific article published on June 2004

Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

scientific article published on 31 August 2009

Visualization of Functional Structure and Kinetic Dynamics of Cellulases

scientific article published on 01 January 2018

List of works by Ryota Iino

A Microfluidic Channel Method for Rapid Drug-Susceptibility Testing of Pseudomonas aeruginosa

A microfluidic device for simple and rapid evaluation of multidrug efflux pump inhibitors

A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate

A single-molecule enzymatic assay in a directly accessible femtoliter droplet array ⬇️

Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization.

Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array

Activation and stiffness of the inhibited states of F1-ATPase probed by single-molecule manipulation.

Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase

Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force.

Correction: Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis

Correlation between the conformational states of F1-ATPase as determined from its crystal structure and single-molecule rotation

Design of a large-scale femtoliter droplet array for single-cell analysis of drug-tolerant and drug-resistant bacteria

Direct observation of intermediate states during the stepping motion of kinesin-1.

Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases

Dynamic structural states of ClpB involved in its disaggregation function.

Evaluation of multidrug efflux pump inhibitors by a new method using microfluidic channels

F1-ATPase: a highly coupled reversible rotary motor

Fluctuation theorem applied to F1-ATPase.

High-speed angle-resolved imaging of a single gold nanorod with microsecond temporal resolution and one-degree angle precision.

High-speed atomic force microscope combined with single-molecule fluorescence microscope.

High-speed atomic force microscopy reveals rotary catalysis of rotorless F₁-ATPase.

High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies

Highly sensitive restriction enzyme assay and analysis: a review.

Intersubunit coordination and cooperativity in ring-shaped NTPases.

Introduction: Molecular Motors

Key chemical factors of arginine finger catalysis of F1-ATPase clarified by an unnatural amino acid mutation.

Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy.

Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria.

Large-scale femtoliter droplet array for digital counting of single biomolecules.

Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single-molecule-fluorescence video imaging.

Mechanism of inhibition by C-terminal alpha-helices of the epsilon subunit of Escherichia coli FoF1-ATP synthase.

Molecular structure and rotary dynamics of Enterococcus hirae V₁-ATPase.

Motion capture and manipulation of a single synthetic molecular rotor by optical microscopy.

Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy

Operation mechanism of F(o) F(1)-adenosine triphosphate synthase revealed by its structure and dynamics

Phosphate release in F1-ATPase catalytic cycle follows ADP release.

Plasmid-Based One-Pot Saturation Mutagenesis and Robot-Based Automated Screening for Protein Engineering.

Principal role of the arginine finger in rotary catalysis of F1-ATPase.

Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin

Publisher Correction: Dynamic structural states of ClpB involved in its disaggregation function

Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis.

Real-time fluorescence visualization of slow tautomerization of single free-base phthalocyanines under ambient conditions.

Rotation and structure of FoF1-ATP synthase.

Rotation of artificial rotor axles in rotary molecular motors.

Rotational mechanism of Enterococcus hirae V1-ATPase by crystal-structure and single-molecule analyses

Simple dark-field microscopy with nanometer spatial precision and microsecond temporal resolution.

Simultaneous Observation of Kinesin-Driven Microtubule Motility and Binding of Adenosine Triphosphate Using Linear Zero-Mode Waveguides

Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface.

Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array

Single-Nanoparticle Tracking with Angstrom Localization Precision and Microsecond Time Resolution

Single-molecule Imaging Analysis of Binding, Processive Movement, and Dissociation of Cellobiohydrolase Trichoderma reesei Cel6A and Its Domains on Crystalline Cellulose.

Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme of Enterococcus hirae V1-ATPase

Single-molecule imaging analysis of elementary reaction steps of Trichoderma reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII

Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens

Single-molecule imaging and manipulation of biomolecular machines and systems.

Small stepping motion of processive dynein revealed by load-free high-speed single-particle tracking

Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus

Temperature-sensitive reaction intermediate of F1-ATPase.

The fence and picket structure of the plasma membrane of live cells as revealed by single molecule techniques (Review).

Torque generation of Enterococcus hirae V-ATPase

Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques

Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

Visualization of Functional Structure and Kinetic Dynamics of Cellulases