List of works - Massimo Stefani

1,2,4-trihydroxynaphthalene-2-O-β-D-glucopyranoside delays amyloid-β42 aggregation and reduces amyloid cytotoxicity.

scientific article published on 26 March 2018

A FTIR microspectroscopy study of the structural and biochemical perturbations induced by natively folded and aggregated transthyretin in HL-1 cardiomyocytes

scientific article published in Scientific Reports

A causative link between the structure of aberrant protein oligomers and their toxicity.

scientific article

A new purified Lawsoniaside remodels amyloid-β42 fibrillation into a less toxic and non-amyloidogenic pathway.

scientific article published on 4 April 2018

A protective role for lipid raft cholesterol against amyloid-induced membrane damage in human neuroblastoma cells

scientific article published on 6 August 2009

A specific nanobody prevents amyloidogenesis of D76N β2-microglobulin in vitro and modifies its tissue distribution in vivo.

scientific article

Acylphosphatase from human skeletal muscle: purification, some properties and levels in normal and myopathic muscles

scientific article published on 01 October 1985

Affinity chromatographic purification of horse muscle acylphosphatase: Evidence of the existence of multiple molecular forms ⬇️

scientific article published on October 15, 1983

Aggregation of the Acylphosphatase from Sulfolobus solfataricus: the folded and partially unfolded states can both be precursors for amyloid formation.

scientific article

Amyloid Aggregation: Role of Biological Membranes and the Aggregate-Membrane System.

scientific article published on 24 January 2014

Arginine-23 is involved in the catalytic site of muscle acylphosphatase.

scientific article published on September 1994

Aspartic-129 is an essential residue in the catalytic mechanism of the low M r phosphotyrosine protein phosphatase

scientific article published on 01 August 1994

Assessing the role of aromatic residues in the amyloid aggregation of human muscle acylphosphatase

scientific article

Beneficial properties of natural phenols: highlight on protection against pathological conditions associated with amyloid aggregation

scientific article published on 03 June 2014

Biochemical and Electrophysiological Modification of Amyloid Transthyretin on Cardiomyocytes

scientific article published on November 2016

Biochemical and biophysical features of both oligomer/fibril and cell membrane in amyloid cytotoxicity.

scientific article

Biological function in a non-native partially folded state of a protein

scientific article published on May 2008

C-terminal region contributes to muscle acylphosphatase three-dimensional structure stabilisation.

scientific article published in April 1996

Ca2+-ATPase from sarcoplasmic reticulum: acylphosphatase activity.

scientific article published in August 1982

Cholesterol in Alzheimer's disease: unresolved questions.

scientific article published on February 2009

Comparison of the folding processes of distantly related proteins. Importance of hydrophobic content in folding.

scientific article published on July 2003

Crystal structure and anion binding in the prokaryotic hydrogenase maturation factor HypF acylphosphatase-like domain

scientific article

Crystallisation of a low molecular weight phosphotyrosine protein phosphatase from bovine liver.

scientific article published in April 1994

Crystallization and preliminary X-ray characterization of the acylphosphatase-like domain from the Escherichia coli hydrogenase maturation factor HypF.

scientific article published on 21 February 2002

Dephosphorylation of tyrosine phosphorylated synthetic peptides by rat liver phosphotyrosine protein phosphatase isoenzymes

scientific journal article

Designing conditions for in vitro formation of amyloid protofilaments and fibrils

scientific article

Different ataxin-3 amyloid aggregates induce intracellular Ca(2+) deregulation by different mechanisms in cerebellar granule cells

scientific article published on 11 September 2013

Differentiation increases the resistance of neuronal cells to amyloid toxicity

scientific article

Differing molecular mechanisms appear to underlie early toxicity of prefibrillar HypF-N aggregates to different cell types

article

Does azurin bind to the transactivation domain of p53? A Trp phosphorescence study

scientific article published on 10 August 2011

Effect of acylphosphates on Ca2+ uptake by sarcoplasmic reticulum vesicles

scientific article published on 01 April 1980

Effect of tetracyclines on the dynamics of formation and destructuration of beta2-microglobulin amyloid fibrils

scientific article

Employing Alzheimer disease animal models for translational research: focus on dietary components

scientific article

Equilibrium unfolding studies of horse muscle acylphosphatase.

scientific article published in November 1994

Expression and turnover of acylphosphatase (muscular isoenzyme) in L6 myoblasts during myogenesis

scientific article published on April 1, 1992

Folding and aggregation are selectively influenced by the conformational preferences of the alpha-helices of muscle acylphosphatase.

scientific article published on 30 July 2001

Generic cell dysfunction in neurodegenerative disorders: role of surfaces in early protein misfolding, aggregation, and aggregate cytotoxicity.

scientific article published on October 2007

Horse brain acylphosphatase: purification and characterization

scientific article published on 01 August 1988

Hydrolysis by horse muscle acylphosphatase of (Ca2+ + Mg2+)-ATPase phosphorylated intermediate.

scientific article published in April 1981

Identification and description of beta-structure in horse muscle acylphosphatase by nuclear magnetic resonance spectroscopy

scientific article published on 01 May 1989

Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases

scientific article (publication date: 4 April 2002)

Insights into the molecular basis of the differing susceptibility of varying cell types to the toxicity of amyloid aggregates.

scientific article

Investigating the effects of mutations on protein aggregation in the cell.

scientific article published on 16 December 2004

Investigation of the effects of copper ions on protein aggregation using a model system.

scientific article published in April 2004

Kinetic partitioning of protein folding and aggregation.

scientific article

Lysozyme interaction with negatively charged lipid bilayers: protein aggregation and membrane fusion

article published in 2012

Mild exposure of RIN-5F β-cells to human islet amyloid polypeptide aggregates upregulates antioxidant enzymes via NADPH oxidase-RAGE: an hormetic stimulus.

scientific article published on 17 December 2013

Molecular insights into cell toxicity of a novel familial amyloidogenic variant of β2-microglobulin

scientific article published on 18 March 2016

Monitoring the process of HypF fibrillization and liposome permeabilization by protofibrils

scientific article published in May 2004

Mutational analysis of acylphosphatase suggests the importance of topology and contact order in protein folding.

scientific article

Natively folded HypF-N and its early amyloid aggregates interact with phospholipid monolayers and destabilize supported phospholipid bilayers

scientific article published on 22 September 2006

Nonspecific interaction of prefibrillar amyloid aggregates with glutamatergic receptors results in Ca2+ increase in primary neuronal cells.

scientific article published in August 2008

Nutraceuticals and amyloid neurodegenerative diseases: a focus on natural phenols.

scientific article

Oleuropein Aglycone: A Possible Drug against Degenerative Conditions. In Vivo Evidence of its Effectiveness against Alzheimer's Disease.

scientific article published on January 2015

Oleuropein aglycon prevents cytotoxic amyloid aggregation of human amylin.

scientific article

Oleuropein aglycone counteracts Aβ42 toxicity in the rat brain.

scientific article published on 7 November 2013

Oleuropein aglycone protects against pyroglutamylated-3 amyloid-ß toxicity: biochemical, epigenetic and functional correlates.

scientific article

Oleuropein aglycone protects transgenic C. elegans strains expressing Aβ42 by reducing plaque load and motor deficit

scientific article

Oleuropein aglycone stabilizes the monomeric α-synuclein and favours the growth of non-toxic aggregates.

scientific article published on 29 May 2018

Oleuropein aglycone: A polyphenol with different targets against amyloid toxicity.

scientific article published on 20 March 2018

Patterns of cell death triggered in two different cell lines by HypF-N prefibrillar aggregates.

scientific article

Prefibrillar amyloid aggregates could be generic toxins in higher organisms.

scientific article published in August 2006

Prefibrillar amyloid protein aggregates share common features of cytotoxicity.

scientific article published on 6 May 2004

Preliminary characterization of two different crystal forms of acylphosphatase from the hyperthermophile archaeon Sulfolobus solfataricus

scientific article

Properties of Cys21-mutated muscle acylphosphatases.

scientific article

Properties of N-terminus truncated and C-terminus mutated muscle acylphosphatases.

scientific article published in April 1995

Protein folding and misfolding on surfaces.

scientific article published on 9 December 2008

Protein misfolding and aggregation: new examples in medicine and biology of the dark side of the protein world

scientific article (publication date: December 2004)

Proteomic analysis of cells exposed to prefibrillar aggregates of HypF-N.

scientific article published on 3 May 2009

Purification and characterization of acylphosphatase erythrocyte isoenzyme from turkey muscle.

scientific article published in October 1990

Rabbit skeletal muscle acylphosphatase: the amino acid sequence of form Ra1

scientific article published on 01 September 1985

Rationalization of the effects of mutations on peptide and protein aggregation rates.

scientific article

Relative influence of hydrophobicity and net charge in the aggregation of two homologous proteins.

scientific article published in December 2003

Replicating neuroblastoma cells in different cell cycle phases display different vulnerability to amyloid toxicity.

scientific article

Reversal of protein aggregation provides evidence for multiple aggregated States

scientific article

Selection of antibody fragments specific for an alpha-helix region of acylphosphatase.

scientific article published in January 2004

Sequence-specific recognition of peptide substrates by the low Mr phosphotyrosine protein phosphatase isoforms.

scientific article published on January 1998

Solution conditions can promote formation of either amyloid protofilaments or mature fibrils from the HypF N-terminal domain

scientific article

Stabilisation of α-helices by site-directed mutagenesis reveals the importance of secondary structure in the transition state for acylphosphatase folding

scientific article published on 01 July 2000

Structural and kinetic investigations on the 15-21 and 42-45 loops of muscle acylphosphatase: evidence for their involvement in enzyme catalysis and conformational stabilization.

scientific article published on June 1997

Structural characterization of the transition state for folding of muscle acylphosphatase.

scientific article published in November 1998

Structural polymorphism of amyloid oligomers and fibrils underlies different fibrillization pathways: immunogenicity and cytotoxicity.

scientific article published on August 2010

Structural, catalytic, and functional properties of low M(r), phosphotyrosine protein phosphatases. Evidence of a long evolutionary history.

scientific article published on February 1997

Structure, conformational stability, and enzymatic properties of acylphosphatase from the hyperthermophile Sulfolobus solfataricus

scientific article

Studying the folding process of the acylphosphatase from Sulfolobus solfataricus. A comparative analysis with other proteins from the same superfamily

scientific article published in July 2004

Synthetic lipid vesicles recruit native-like aggregates and affect the aggregation process of the prion Ure2p: insights on vesicle permeabilization and charge selectivity.

scientific article published on April 2009

The (1-63) region of the p53 transactivation domain aggregates in vitro into cytotoxic amyloid assemblies.

scientific article published on 16 January 2008

The intrachain disulfide bridge is responsible of the unusual stability properties of novel acylphosphatase from Escherichia coli

scientific article published on 28 November 2006

The polyphenol Oleuropein aglycone hinders the growth of toxic transthyretin amyloid assemblies.

scientific article published on 12 January 2016

The polyphenol oleuropein aglycone protects TgCRND8 mice against Aß plaque pathology

scientific article

The yeast prion Ure2p native-like assemblies are toxic to mammalian cells regardless of their aggregation state.

scientific article published on 29 March 2006

Thermodynamics and kinetics of folding of common-type acylphosphatase: comparison to the highly homologous muscle isoenzyme.

scientific article published in February 1999

Three-dimensional structural characterization of a novel Drosophila melanogaster acylphosphatase.

scientific article

Toxic effects of amyloid fibrils on cell membranes: the importance of ganglioside GM1.

scientific article

List of works by Massimo Stefani

1,2,4-trihydroxynaphthalene-2-O-β-D-glucopyranoside delays amyloid-β42 aggregation and reduces amyloid cytotoxicity.

A FTIR microspectroscopy study of the structural and biochemical perturbations induced by natively folded and aggregated transthyretin in HL-1 cardiomyocytes

A causative link between the structure of aberrant protein oligomers and their toxicity.

A new purified Lawsoniaside remodels amyloid-β42 fibrillation into a less toxic and non-amyloidogenic pathway.

A protective role for lipid raft cholesterol against amyloid-induced membrane damage in human neuroblastoma cells

A specific nanobody prevents amyloidogenesis of D76N β2-microglobulin in vitro and modifies its tissue distribution in vivo.

Acylphosphatase from human skeletal muscle: purification, some properties and levels in normal and myopathic muscles

Affinity chromatographic purification of horse muscle acylphosphatase: Evidence of the existence of multiple molecular forms ⬇️

Aggregation of the Acylphosphatase from Sulfolobus solfataricus: the folded and partially unfolded states can both be precursors for amyloid formation.

Amyloid Aggregation: Role of Biological Membranes and the Aggregate-Membrane System.

Arginine-23 is involved in the catalytic site of muscle acylphosphatase.

Aspartic-129 is an essential residue in the catalytic mechanism of the low M r phosphotyrosine protein phosphatase

Assessing the role of aromatic residues in the amyloid aggregation of human muscle acylphosphatase

Beneficial properties of natural phenols: highlight on protection against pathological conditions associated with amyloid aggregation

Biochemical and Electrophysiological Modification of Amyloid Transthyretin on Cardiomyocytes

Biochemical and biophysical features of both oligomer/fibril and cell membrane in amyloid cytotoxicity.

Biological function in a non-native partially folded state of a protein

C-terminal region contributes to muscle acylphosphatase three-dimensional structure stabilisation.

Ca2+-ATPase from sarcoplasmic reticulum: acylphosphatase activity.

Cholesterol in Alzheimer's disease: unresolved questions.

Comparison of the folding processes of distantly related proteins. Importance of hydrophobic content in folding.

Crystal structure and anion binding in the prokaryotic hydrogenase maturation factor HypF acylphosphatase-like domain

Crystallisation of a low molecular weight phosphotyrosine protein phosphatase from bovine liver.

Crystallization and preliminary X-ray characterization of the acylphosphatase-like domain from the Escherichia coli hydrogenase maturation factor HypF.

Dephosphorylation of tyrosine phosphorylated synthetic peptides by rat liver phosphotyrosine protein phosphatase isoenzymes

Designing conditions for in vitro formation of amyloid protofilaments and fibrils

Different ataxin-3 amyloid aggregates induce intracellular Ca(2+) deregulation by different mechanisms in cerebellar granule cells

Differentiation increases the resistance of neuronal cells to amyloid toxicity

Differing molecular mechanisms appear to underlie early toxicity of prefibrillar HypF-N aggregates to different cell types

Does azurin bind to the transactivation domain of p53? A Trp phosphorescence study

Effect of acylphosphates on Ca2+ uptake by sarcoplasmic reticulum vesicles

Effect of tetracyclines on the dynamics of formation and destructuration of beta2-microglobulin amyloid fibrils

Employing Alzheimer disease animal models for translational research: focus on dietary components

Equilibrium unfolding studies of horse muscle acylphosphatase.

Expression and turnover of acylphosphatase (muscular isoenzyme) in L6 myoblasts during myogenesis

Folding and aggregation are selectively influenced by the conformational preferences of the alpha-helices of muscle acylphosphatase.

Generic cell dysfunction in neurodegenerative disorders: role of surfaces in early protein misfolding, aggregation, and aggregate cytotoxicity.

Horse brain acylphosphatase: purification and characterization

Hydrolysis by horse muscle acylphosphatase of (Ca2+ + Mg2+)-ATPase phosphorylated intermediate.

Identification and description of beta-structure in horse muscle acylphosphatase by nuclear magnetic resonance spectroscopy

Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases

Insights into the molecular basis of the differing susceptibility of varying cell types to the toxicity of amyloid aggregates.

Investigating the effects of mutations on protein aggregation in the cell.

Investigation of the effects of copper ions on protein aggregation using a model system.

Kinetic partitioning of protein folding and aggregation.

Lysozyme interaction with negatively charged lipid bilayers: protein aggregation and membrane fusion

Mild exposure of RIN-5F β-cells to human islet amyloid polypeptide aggregates upregulates antioxidant enzymes via NADPH oxidase-RAGE: an hormetic stimulus.

Molecular insights into cell toxicity of a novel familial amyloidogenic variant of β2-microglobulin

Monitoring the process of HypF fibrillization and liposome permeabilization by protofibrils

Mutational analysis of acylphosphatase suggests the importance of topology and contact order in protein folding.

Natively folded HypF-N and its early amyloid aggregates interact with phospholipid monolayers and destabilize supported phospholipid bilayers

Nonspecific interaction of prefibrillar amyloid aggregates with glutamatergic receptors results in Ca2+ increase in primary neuronal cells.

Nutraceuticals and amyloid neurodegenerative diseases: a focus on natural phenols.

Oleuropein Aglycone: A Possible Drug against Degenerative Conditions. In Vivo Evidence of its Effectiveness against Alzheimer's Disease.

Oleuropein aglycon prevents cytotoxic amyloid aggregation of human amylin.

Oleuropein aglycone counteracts Aβ42 toxicity in the rat brain.

Oleuropein aglycone protects against pyroglutamylated-3 amyloid-ß toxicity: biochemical, epigenetic and functional correlates.

Oleuropein aglycone protects transgenic C. elegans strains expressing Aβ42 by reducing plaque load and motor deficit

Oleuropein aglycone stabilizes the monomeric α-synuclein and favours the growth of non-toxic aggregates.

Oleuropein aglycone: A polyphenol with different targets against amyloid toxicity.

Patterns of cell death triggered in two different cell lines by HypF-N prefibrillar aggregates.

Prefibrillar amyloid aggregates could be generic toxins in higher organisms.

Prefibrillar amyloid protein aggregates share common features of cytotoxicity.

Preliminary characterization of two different crystal forms of acylphosphatase from the hyperthermophile archaeon Sulfolobus solfataricus

Properties of Cys21-mutated muscle acylphosphatases.

Properties of N-terminus truncated and C-terminus mutated muscle acylphosphatases.

Protein folding and misfolding on surfaces.

Protein misfolding and aggregation: new examples in medicine and biology of the dark side of the protein world

Proteomic analysis of cells exposed to prefibrillar aggregates of HypF-N.

Purification and characterization of acylphosphatase erythrocyte isoenzyme from turkey muscle.

Rabbit skeletal muscle acylphosphatase: the amino acid sequence of form Ra1

Rationalization of the effects of mutations on peptide and protein aggregation rates.

Relative influence of hydrophobicity and net charge in the aggregation of two homologous proteins.

Replicating neuroblastoma cells in different cell cycle phases display different vulnerability to amyloid toxicity.

Reversal of protein aggregation provides evidence for multiple aggregated States

Selection of antibody fragments specific for an alpha-helix region of acylphosphatase.

Sequence-specific recognition of peptide substrates by the low Mr phosphotyrosine protein phosphatase isoforms.

Solution conditions can promote formation of either amyloid protofilaments or mature fibrils from the HypF N-terminal domain

Stabilisation of α-helices by site-directed mutagenesis reveals the importance of secondary structure in the transition state for acylphosphatase folding