List of works - Pimchai Chaiyen

3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover

scientific article

A Chemo-Enzymatic Cascade for the Smart Detection of Nitro- and Halogenated Phenols

scientific article published on 05 August 2019

A Conserved Active-site Threonine Is Important for Both Sugar and Flavin Oxidations of Pyranose 2-Oxidase

scientific article

A Single-Site Mutation at Ser146 Expands the Reactivity of the Oxygenase Component of p-Hydroxyphenylacetate 3-Hydroxylase

scientific article published on 19 August 2016

A complete bioconversion cascade for dehalogenation and denitration by bacterial flavin-dependent enzymes

scientific article published in Journal of Biological Chemistry

A flap motif in human serine hydroxymethyltransferase is important for structural stabilization, ligand binding, and control of product release

scientific article published on 22 May 2019

A novel two-protein component flavoprotein hydroxylase. p-Hydroxyphenylacetate hydroxylase from Acinetobacter baumannii

scholarly article by Pimchai Chaiyen published in November 2001

Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway

scientific article published on 10 June 2019

An Experiment Illustrating the Change in Ligand pKa upon Protein Binding

Antimalarial Inhibitors Targeting Serine Hydroxymethyltransferase (SHMT) with in Vivo Efficacy and Analysis of their Binding Mode Based on X-ray Cocrystal Structures

scientific article

Applications of Planar ITO Electrodes for Studying of Some Biochemical Activities

Biotransformation of Plant Derived Phenolic Acids

scientific article published on 26 December 2017

Characterization of Plasmodium falciparum serine hydroxymethyltransferase-A potential antimalarial target

scientific article (publication date: November 2009)

Cloning and expression of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii: evidence of the divergence of enzymes in the class of two-protein component aromatic hydroxylases

scientific article published in October 2004

Conformational Aspects in the Design of Inhibitors for Serine Hydroxymethyltransferase (SHMT): Biphenyl, Aryl Sulfonamide, and Aryl Sulfone Motifs.

scientific article

Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics

article

Control of C4a-hydroperoxyflavin protonation in the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase

scientific article published on 17 June 2014

Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii

scientific article

Crystallization and preliminary X-ray crystallographic analysis of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase from Pseudomonas sp. MA-1.

scientific article published on 24 February 2005

Detection of a C4a-Hydroperoxyflavin Intermediate in the Reaction of a Flavoprotein Oxidase†

scientific article published on 25 July 2008

Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent

scientific article

Dissecting the low catalytic capability of flavin-dependent halogenases

scientific article published on 13 November 2020

Distinct biochemical properties of human serine hydroxymethyltransferase compared with the Plasmodium enzyme: implications for selective inhibition

scientific article

Engineering of pyranose 2-oxidase: improvement for biofuel cell and food applications through semi-rational protein design

scientific article

Enzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalyst

scientific article published in February 2018

Enzymes in the p-hydroxyphenylacetate degradation pathway of Acinetobacter baumannii

scholarly article by Kittisak Thotsaporn et al published December 2016 in Journal of Molecular Catalysis B: Enzymatic

Flavoenzymes catalyzing oxidative aromatic ring-cleavage reactions

scientific article published on September 2009

Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase

Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

scientific article published on July 1997

Green and sustainable biocatalytic production of 3,4,5-trihydroxycinnamic acid from palm oil mill effluent

scientific article published in 2017

H-bonding and positive charge at the N5/O4 locus are critical for covalent flavin attachment in trametes pyranose 2-oxidase

scientific article

Human and Plasmodium serine hydroxymethyltransferases differ in rate-limiting steps and pH-dependent substrate inhibition behavior

scientific article published on 28 July 2017

Hydrogen peroxide elimination from C4a-hydroperoxyflavin in a flavoprotein oxidase occurs through a single proton transfer from flavin N5 to a peroxide leaving group

scientific article

Hydroxylation of 4-hydroxyphenylethylamine derivatives by R263 variants of the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase

scientific article

Identification of a Catalytic Base for Sugar Oxidation in the Pyranose 2-Oxidase Reaction

scientific article published on 19 October 2011

Identification of a Hotspot Residue for Improving the Thermostability of a Flavin-Dependent Monooxygenase

scientific article published on 26 September 2019

Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers: A novel heterogeneous catalyst

Inhibitors of plasmodial serine hydroxymethyltransferase (SHMT): cocrystal structures of pyrazolopyrans with potent blood- and liver-stage activities

scientific article

Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase

scientific article published on 3 November 2011

Kinetic Mechanism of the Dechlorinating Flavin-dependent Monooxygenase HadA.

scientific article published on 3 February 2017

Kinetic Mechanisms of the Oxygenase from a Two-component Enzyme,p-Hydroxyphenylacetate 3-Hydroxylase fromAcinetobacter baumannii

scientific article published in Journal of Biological Chemistry

Kinetic isotope effects on the noncovalent flavin mutant protein of pyranose 2-oxidase reveal insights into the flavin reduction mechanism

scientific article published in May 2010

Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase

scientific article

Kinetic mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae

scientific article published on 25 February 2015

Kinetic mechanism of pyranose 2-oxidase from trametes multicolor

scientific article published in May 2009

Kinetics of a Two-Componentp-Hydroxyphenylacetate Hydroxylase Explain How Reduced Flavin Is Transferred from the Reductase to the Oxygenase†

scientific article published in 2007

Luciferase from Vibrio campbellii is more thermostable and binds reduced FMN better than its homologues

scientific article published on 30 August 2007

LuxG is a functioning flavin reductase for bacterial luminescence

scientific article

Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes

scientific article published on February 2015

Measuring Binding Affinity of Protein−Ligand Interaction Using Spectrophotometry: Binding of Neutral Red to Riboflavin-Binding Protein

Mechanism of Oxygen Activation in a Flavin-Dependent Monooxygenase: A Nearly Barrierless Formation of C4a-Hydroperoxyflavin via Proton-Coupled Electron Transfer

scientific article published on 20 July 2015

Mechanisms of reduced flavin transfer in the two-component flavin-dependent monooxygenases

scientific article published on 20 May 2014

Mechanistic Insights into the Dual Activities of the Single Active Site of L-Lysine Oxidase/Monooxygenase from Pseudomonas sp. AIU 813

scientific article published on 11 June 2020

Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions

scientific article published on 29 September 2019

Multiple pathways guide oxygen diffusion into flavoenzyme active sites

scientific article

One-Pot Bioconversion of l -Arabinose to l -Ribulose in an Enzymatic Cascade

scientific article published on 29 January 2019

Oxidation mode of pyranose 2-oxidase is controlled by pH.

scientific article

Oxidative dehalogenation and denitration by a flavin-dependent monooxygenase is controlled by substrate deprotonation

scientific article published on 08 August 2018

Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay

scientific article

Proton-coupled electron transfer and adduct configuration are important for C4a-hydroperoxyflavin formation and stabilization in a flavoenzyme

scientific article

Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation

scientific journal article

Rapid kinetics reveal surprising flavin chemistry in the bifurcating electron transfer flavoprotein from Acidaminococcus fermentans

scientific article published on 25 November 2020

Reaction of 2-Methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) Oxygenase withN-Methyl-5-hydroxynicotinic acid: Studies on the Mode of Binding, and Protonation Status of the Substrate†

scientific article published on 01 November 1997

Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates

scientific article published on 18 August 2015

Reduction kinetics of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1.

scientific article published on 17 May 2012

Reduction kinetics of a flavin oxidoreductase LuxG from Photobacterium leiognathi (TH1): half-sites reactivity

scientific article

Selectivity of substrate binding and ionization of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

scientific article published on 2 February 2015

Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues.

scientific article published on 22 June 2009

Special Issue: Flavins and Flavoproteins

scientific article published on 17 July 2015

Stabilization of C4a-hydroperoxyflavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms

scientific article published on 16 June 2011

Structural Basis for Substrate Binding and Regioselective Oxidation of Monosaccharides at C3 by Pyranose 2-Oxidase

scientific article published in Journal of Biological Chemistry

Structure and proposed mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae

scientific article

Structure of the monooxygenase component of a two-component flavoprotein monooxygenase

scientific article

Structure, Mechanism, and Mutation of Bacterial Luciferase

scientific article published on 7 December 2014

Structures of Plasmodium vivax serine hydroxymethyltransferase: implications for ligand-binding specificity and functional control.

scientific article

Studies on the transglucosylation reactions of cassava and Thai rosewood beta-glucosidases using 2-deoxy-2-fluoro-glycosyl-enzyme intermediates

scientific article published in October 2005

The 1.6 Å Crystal Structure of Pyranose Dehydrogenase from Agaricus meleagris Rationalizes Substrate Specificity and Reveals a Flavin Intermediate

scientific article

The C-terminal domain of 4-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii is an autoinhibitory domain

scientific article published on 3 June 2012

The enigmatic reaction of flavins with oxygen

scientific article published on 20 July 2012

The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter

article

The reaction kinetics of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1 provide an understanding of the para-hydroxylation enzyme catalytic cycle.

scientific article published on 15 October 2013

The reductase of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii requires p-hydroxyphenylacetate for effective catalysis

scientific article published in August 2005

The structure of Plasmodium falciparum serine hydroxymethyltransferase reveals a novel redox switch that regulates its activities.

scientific article

The substrate oxidation mechanism of pyranose 2-oxidase and other related enzymes in the glucose-methanol-choline superfamily

scientific article published on 10 May 2013

The transfer of reduced flavin mononucleotide from LuxG oxidoreductase to luciferase occurs via free diffusion

scientific article

Thermodynamics and Reduction Kinetics Properties of 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase†

scientific article published on 01 March 1997

Time-resolved Stokes shift in proteins with continuum model: Slow dynamics in proteins

Tuning of pKa values activates substrates in flavin-dependent aromatic hydroxylases

scientific article published on 02 February 2020

Tyr217 and His213 are important for substrate binding and hydroxylation of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1

scientific article published on 27 January 2016

Ultrafast fluorescence dynamics of flavin adenine dinucleotide in pyranose 2-oxidases variants and their complexes with acetate: Conformational heterogeneity with different dielectric constants

article

Ultrafast solvation dynamics of flavin mononucleotide in the reductase component of p-hydroxyphenylacetate hydroxylase.

scientific article published in June 2009

Unusual Mechanism of Oxygen Atom Transfer and Product Rearrangement in the Catalytic Reaction of 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase†

scientific article published on 01 July 1997

Use of 8-substituted-FAD analogues to investigate the hydroxylation mechanism of the flavoprotein 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

scientific article published in April 2004

p-Hydroxyphenylacetate 3-Hydroxylase as a Biocatalyst for the Synthesis of Trihydroxyphenolic Acids

article published in 2015

pH-dependent studies reveal an efficient hydroxylation mechanism of the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase

scientific article published on 28 October 2010

List of works by Pimchai Chaiyen

3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover

A Chemo-Enzymatic Cascade for the Smart Detection of Nitro- and Halogenated Phenols

A Conserved Active-site Threonine Is Important for Both Sugar and Flavin Oxidations of Pyranose 2-Oxidase

A Single-Site Mutation at Ser146 Expands the Reactivity of the Oxygenase Component of p-Hydroxyphenylacetate 3-Hydroxylase

A complete bioconversion cascade for dehalogenation and denitration by bacterial flavin-dependent enzymes

A flap motif in human serine hydroxymethyltransferase is important for structural stabilization, ligand binding, and control of product release

A novel two-protein component flavoprotein hydroxylase. p-Hydroxyphenylacetate hydroxylase from Acinetobacter baumannii

Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway

An Experiment Illustrating the Change in Ligand pKa upon Protein Binding

Antimalarial Inhibitors Targeting Serine Hydroxymethyltransferase (SHMT) with in Vivo Efficacy and Analysis of their Binding Mode Based on X-ray Cocrystal Structures

Applications of Planar ITO Electrodes for Studying of Some Biochemical Activities

Biotransformation of Plant Derived Phenolic Acids

Characterization of Plasmodium falciparum serine hydroxymethyltransferase-A potential antimalarial target

Cloning and expression of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii: evidence of the divergence of enzymes in the class of two-protein component aromatic hydroxylases

Conformational Aspects in the Design of Inhibitors for Serine Hydroxymethyltransferase (SHMT): Biphenyl, Aryl Sulfonamide, and Aryl Sulfone Motifs.

Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics

Control of C4a-hydroperoxyflavin protonation in the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase

Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii

Crystallization and preliminary X-ray crystallographic analysis of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase from Pseudomonas sp. MA-1.

Detection of a C4a-Hydroperoxyflavin Intermediate in the Reaction of a Flavoprotein Oxidase†

Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent

Dissecting the low catalytic capability of flavin-dependent halogenases

Distinct biochemical properties of human serine hydroxymethyltransferase compared with the Plasmodium enzyme: implications for selective inhibition

Engineering of pyranose 2-oxidase: improvement for biofuel cell and food applications through semi-rational protein design

Enzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalyst

Enzymes in the p-hydroxyphenylacetate degradation pathway of Acinetobacter baumannii

Flavoenzymes catalyzing oxidative aromatic ring-cleavage reactions

Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase

Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

Green and sustainable biocatalytic production of 3,4,5-trihydroxycinnamic acid from palm oil mill effluent

H-bonding and positive charge at the N5/O4 locus are critical for covalent flavin attachment in trametes pyranose 2-oxidase

Human and Plasmodium serine hydroxymethyltransferases differ in rate-limiting steps and pH-dependent substrate inhibition behavior

Hydrogen peroxide elimination from C4a-hydroperoxyflavin in a flavoprotein oxidase occurs through a single proton transfer from flavin N5 to a peroxide leaving group

Hydroxylation of 4-hydroxyphenylethylamine derivatives by R263 variants of the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase

Identification of a Catalytic Base for Sugar Oxidation in the Pyranose 2-Oxidase Reaction

Identification of a Hotspot Residue for Improving the Thermostability of a Flavin-Dependent Monooxygenase

Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers: A novel heterogeneous catalyst

Inhibitors of plasmodial serine hydroxymethyltransferase (SHMT): cocrystal structures of pyrazolopyrans with potent blood- and liver-stage activities

Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase

Kinetic Mechanism of the Dechlorinating Flavin-dependent Monooxygenase HadA.

Kinetic Mechanisms of the Oxygenase from a Two-component Enzyme,p-Hydroxyphenylacetate 3-Hydroxylase fromAcinetobacter baumannii

Kinetic isotope effects on the noncovalent flavin mutant protein of pyranose 2-oxidase reveal insights into the flavin reduction mechanism

Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase

Kinetic mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae

Kinetic mechanism of pyranose 2-oxidase from trametes multicolor

Kinetics of a Two-Componentp-Hydroxyphenylacetate Hydroxylase Explain How Reduced Flavin Is Transferred from the Reductase to the Oxygenase†

Luciferase from Vibrio campbellii is more thermostable and binds reduced FMN better than its homologues

LuxG is a functioning flavin reductase for bacterial luminescence

Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes

Measuring Binding Affinity of Protein−Ligand Interaction Using Spectrophotometry: Binding of Neutral Red to Riboflavin-Binding Protein

Mechanism of Oxygen Activation in a Flavin-Dependent Monooxygenase: A Nearly Barrierless Formation of C4a-Hydroperoxyflavin via Proton-Coupled Electron Transfer

Mechanisms of reduced flavin transfer in the two-component flavin-dependent monooxygenases

Mechanistic Insights into the Dual Activities of the Single Active Site of L-Lysine Oxidase/Monooxygenase from Pseudomonas sp. AIU 813

Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions

Multiple pathways guide oxygen diffusion into flavoenzyme active sites

One-Pot Bioconversion of l -Arabinose to l -Ribulose in an Enzymatic Cascade

Oxidation mode of pyranose 2-oxidase is controlled by pH.

Oxidative dehalogenation and denitration by a flavin-dependent monooxygenase is controlled by substrate deprotonation

Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay

Proton-coupled electron transfer and adduct configuration are important for C4a-hydroperoxyflavin formation and stabilization in a flavoenzyme

Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation

Rapid kinetics reveal surprising flavin chemistry in the bifurcating electron transfer flavoprotein from Acidaminococcus fermentans

Reaction of 2-Methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) Oxygenase withN-Methyl-5-hydroxynicotinic acid: Studies on the Mode of Binding, and Protonation Status of the Substrate†

Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates

Reduction kinetics of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1.

Reduction kinetics of a flavin oxidoreductase LuxG from Photobacterium leiognathi (TH1): half-sites reactivity

Selectivity of substrate binding and ionization of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues.

Special Issue: Flavins and Flavoproteins

Stabilization of C4a-hydroperoxyflavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms

Structural Basis for Substrate Binding and Regioselective Oxidation of Monosaccharides at C3 by Pyranose 2-Oxidase

Structure and proposed mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae

Structure of the monooxygenase component of a two-component flavoprotein monooxygenase

Structure, Mechanism, and Mutation of Bacterial Luciferase

Structures of Plasmodium vivax serine hydroxymethyltransferase: implications for ligand-binding specificity and functional control.

Studies on the transglucosylation reactions of cassava and Thai rosewood beta-glucosidases using 2-deoxy-2-fluoro-glycosyl-enzyme intermediates

The 1.6 Å Crystal Structure of Pyranose Dehydrogenase from Agaricus meleagris Rationalizes Substrate Specificity and Reveals a Flavin Intermediate

The C-terminal domain of 4-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii is an autoinhibitory domain

The enigmatic reaction of flavins with oxygen