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List of works by David Roper

A de novo designed inhibitor of D-Ala-D-Ala ligase from E. coli

scientific article published in October 2005

A model of membrane contraction predicting initiation and completion of bacterial cell division

scientific article

A road map for prioritizing warheads for cysteine targeting covalent inhibitors

scientific article published on 06 October 2018

Active membrane transport and receptor proteins from bacteria

scientific article

Adenosine phosphonate inhibitors of lipid II: Alanyl tRNA ligase MurM from Streptococcus pneumoniae

scientific article published on 25 May 2007

Adenosine tetraphosphoadenosine drives a continuous ATP-release assay for aminoacyl-tRNA synthetases and other adenylate-forming enzymes ⬇️

scientific article published on 13 August 2013

Allosteric activation in Bacillus stearothermophilus lactate dehydrogenase investigated by an X-ray crystallographic analysis of a mutant designed to prevent tetramerization of the enzyme.

scientific article

An analysis of relative costs and potential benefits of different policies for antenatal screening for beta thalassaemia trait and variant haemoglobins

scientific article published on September 1999

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

scientific article published in Scientific Reports

Anticancer metallohelices: nanomolar potency and high selectivity

scientific article published on 26 October 2015

Asymmetric triplex metallohelices with high and selective activity against cancer cells.

scientific article

Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities

scientific article published on 06 June 2019

Bacterial cell division: experimental and theoretical approaches to the divisome

scientific article

Bacterial cell wall assembly: still an attractive antibacterial target

scientific article published on 12 January 2011

Beyond the Discovery Void: New targets for antibacterial compounds.

scientific article published in June 2016

Biological Insights from a Simulation Model of the Critical FtsZ Accumulation Required for Prokaryotic Cell Division.

scientific article published on 10 June 2015

CO₂directly modulates connexin 26 by formation of carbamate bridges between subunits

scientific article published on 12 November 2013

Carbohydrate scaffolds as glycosyltransferase inhibitors with in vivo antibacterial activity

scientific article published on 21 July 2015

Characterization of tRNA-dependent peptide bond formation by MurM in the synthesis of Streptococcus pneumoniae peptidoglycan

scientific article published on 12 December 2007

Circular dichroism spectroscopy for the study of protein-ligand interactions.

scientific article published in January 2005

Core Steps of Membrane-Bound Peptidoglycan Biosynthesis: Recent Advances, Insight and Opportunities

scientific article

Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12

scientific article

Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics.

scientific article

Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms

scientific article

Crystal structures of penicillin-binding proteins 4 and 5 from Haemophilus influenzae

scientific article

Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli

scientific article

Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa.

scientific article published on 30 April 2013

Crystallization and preliminary X-ray analysis of a bifunctional enzyme: HHDD isomerase/OPET decarboxylase from Escherichia coli

scientific article published on 01 September 1996

Crystallization and preliminary X-ray characterization of VanA from Enterococcus faecium BM4147: towards the molecular basis of bacterial resistance to the glycopeptide antibiotic vancomycin.

scientific article

Design and synthesis of new hydroxyethylamines as inhibitors of D-alanyl-D-lactate ligase (VanA) and D-alanyl-D-alanine ligase (DdlB).

scientific article published on 19 January 2009

Diaryltriazenes as antibacterial agents against methicillin resistant Staphylococcus aureus (MRSA) and Mycobacterium smegmatis.

scientific article published on 31 December 2016

Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases

scientific article

Evaluation of the utility of the HemoCue 301 haemoglobinometer for blood donor screening

scientific article published on 01 July 2007

Expression, purification and characterisation of soluble GlfT and the identification of a novel galactofuranosyltransferase Rv3782 involved in priming GlfT-mediated galactan polymerisation in Mycobacterium tuberculosis

scientific journal article

Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C.

scientific article published on 30 September 2006

Expression, purification and crystallization of the cell-division protein YgfE from Escherichia coli.

scientific article

Expression, purification and preliminary crystallographic analysis of 2,4-dihydroxy-hepta-2-ene-1,7-dioate aldolase (HpcH) from Escherichia coli C.

scientific article published on 31 August 2005

Expression, purification, crystallization and preliminary characterization of an HHED aldolase homologue from Escherichia coli K12.

scientific article published on 23 November 2002

Expression, purification, crystallization and preliminary characterization of uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase (MurE) from Streptococcus pneumoniae 110K/70.

scientific article published on 23 January 2004

Fluorescent reagents for in vitro studies of lipid-linked steps of bacterial peptidoglycan biosynthesis: derivatives of UDPMurNAc-pentapeptide containing d-cysteine at position 4 or 5.

scientific article published on 7 September 2006

FtsZ polymer-bundling by the Escherichia coli ZapA orthologue, YgfE, involves a conformational change in bound GTP.

scientific article published on 19 March 2007

Full-length structure of the major autolysin LytA

scientific article

Identification of a novel inhibition site in translocase MraY based upon the site of interaction with lysis protein E from bacteriophage ϕX174.

scientific article published on 4 June 2014

In silico identification, synthesis and biological evaluation of novel tetrazole inhibitors of MurB.

scientific article published on 24 January 2018

In vitro Reconstitution of Peptidoglycan Assembly from the Gram-Positive Pathogen Streptococcus pneumoniae

scientific article published on 30 September 2013

In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA).

scientific article

Indistinguishability and identifiability of kinetic models for the Mur C reaction in peptidoglycan biosynthesis* *EPSRC funded project Indistinguishability analysis for model discrimination in Systems Biology: A Feasibility Study applied to Bacterial

Indistinguishability and identifiability of kinetic models for the MurC reaction in peptidoglycan biosynthesis

scientific article published on 01 September 2010

Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine. ⬇️

scientific article published on 5 December 2017

Inhibition of tRNA-dependent ligase MurM from Streptococcus pneumoniae by phosphonate and sulfonamide inhibitors

scientific article

Insights into bacterial cell division from a structure of EnvC bound to the FtsX periplasmic domain

scientific article published on 23 October 2020

Kinetic characterization of lipid II-Ala:alanyl-tRNA ligase (MurN) from Streptococcus pneumoniae using semisynthetic aminoacyl-lipid II substrates.

scientific article published on 8 October 2008

Mechanisms of Incorporation for D-Amino Acid Probes That Target Peptidoglycan Biosynthesis

scientific article published on 05 December 2019

Metallohelices that kill Gram-negative pathogens using intracellular antimicrobial peptide pathways

scientific article published on 05 September 2019

Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4.

scientific article

Mutations in the DNA mismatch repair proteins MutS and MutL of oxazolidinone-resistant or -susceptible Enterococcus faecium

scientific article

Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ

scientific article published on 10 December 2019

Nod1 signaling overcomes resistance of S. pneumoniae to opsonophagocytic killing

scientific article

Observation of the time-course for peptidoglycan lipid intermediate II polymerization by Staphylococcus aureus monofunctional transglycosylase

scientific article published on 23 May 2014

PhD students

scientific article published on 01 March 1990

Phospho-MurNAc-pentapeptide translocase (MraY) as a target for antibacterial agents and antibacterial proteins

scientific article published on June 2006

Preliminary crystallographic analysis of 4-oxalocrotonate tautomerase reveals the oligomeric structure of the enzyme

scientific article published on 01 November 1994

Preliminary crystallographic analysis of 5-carboxymethyl-2-hydroxymuconate isomerase from Escherichia coli.

scientific article published on December 1989

Prospects for novel inhibitors of peptidoglycan transglycosylases

scientific article

Pseudomonas aeruginosa MurE amide ligase: enzyme kinetics and peptide inhibitor

scientific article

Purification, nucleotide sequence and some properties of a bifunctional isomerase/decarboxylase from the homoprotocatechuate degradative pathway of Escherichia coli C.

scientific article published on October 1993

Purification, some properties and nucleotide sequence of 5-carboxymethyl-2-hydroxymuconate isomerase of Escherichia coli C.

scientific article published on June 1990

Reconstruction of diaminopimelic acid biosynthesis allows characterisation of Mycobacterium tuberculosis N-succinyl-L,L-diaminopimelic acid desuccinylase.

scientific article published on 15 March 2016

Refinement of Haemophilus influenzae diaminopimelic acid epimerase (DapF) at 1.75 A resolution suggests a mechanism for stereocontrol during catalysis

scientific article

SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis

scientific article published on 28 May 2019

Sansanmycin natural product analogues as potent and selective anti-mycobacterials that inhibit lipid I biosynthesis

scientific article published on March 2017

Sequence of the hpcC and hpcG genes of the meta-fission homoprotocatechuic acid pathway of Escherichia coli C: nearly 40% amino-acid identity with the analogous enzymes of the catechol pathway

scientific article published on April 1995

Solution structure and interactions of the Escherichia coli cell division activator protein CedA.

scientific article published in May 2005

Specificity Determinants for Lysine Incorporation in Staphylococcus aureus Peptidoglycan as Revealed by the Structure of a MurE Enzyme Ternary Complex

scientific article

Structural and mechanistic studies of the orf12 gene product from the clavulanic acid biosynthesis pathway

scientific article

Structural basis of lipopolysaccharide maturation by the O-antigen ligase

scientific article published on 6 April 2022

Structural characterisation of the insecticidal toxin XptA1, reveals a 1.15 MDa tetramer with a cage-like structure.

scientific article published on 23 December 2006

Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli

scientific article

Structure and mechanism of HpcH: a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli

scientific article

Structure of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localization

scientific article published on 14 December 2020

Structure of the diaminopimelate epimerase DapF from Mycobacterium tuberculosis

scientific article

Structure-Guided Enhancement of Selectivity of Chemical Probe Inhibitors Targeting Bacterial Seryl-tRNA Synthetase

scientific article published on 05 November 2019

Structure-based modeling and dynamics of MurM, a Streptococcus pneumoniae penicillin resistance determinant present at the cytoplasmic membrane

scientific article published on 18 March 2021

Structure/function studies on a S-adenosyl-L-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis

scientific article published in November 2004

Subcloning and nucleotide sequence of the 3,4-dihydroxyphenylacetate (homoprotocatechuate) 2,3-dioxygenase gene from Escherichia coli C.

scientific article published on November 1990

Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity

scientific article published on 6 January 2017

Substrate and Stereochemical Control of Peptidoglycan Cross-Linking by Transpeptidation by Escherichia coli PBP1B

scientific article published on 02 March 2020

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a.

scientific article

Synthesis and in vitro enzyme activity of an oxa analogue of azi-DAP

Tetramerization of ZapA is required for FtsZ bundling.

scientific article published in February 2013

The Escherichia coli C homoprotocatechuate degradative operon: hpc gene order, direction of transcription and control of expression

scientific article published on February 1993

The Extracellular Domain of Two-component System Sensor Kinase VanS from Streptomyces coelicolor Binds Vancomycin at a Newly Identified Binding Site

scientific article published on 31 March 2020

The N-Terminal Domain of Human Topoisomerase IIα Is a DNA-Dependent ATPase†

scientific article published on 01 December 1998

The crystal structure and oligomeric form of Escherichia colil,d-carboxypeptidase A

scientific article published on 05 April 2018

The crystal structure of HpcE, a bifunctional decarboxylase/isomerase with a multifunctional fold

scientific article

The mechanics of FtsZ fibers

scientific article published on 21 February 2012

The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA)

scientific article published in August 2000

The pH dependence of polymerization and bundling by the essential bacterial cytoskeletal protein FtsZ

scientific article

The physico-chemical characterization of a boiling stable antifreeze protein from a perennial grass (Lolium perenne).

scientific article published in February 2003

The role of the jaw subdomain of peptidoglycan glycosyltransferases for lipid II polymerization

scientific article published on 01 June 2018

The structural consequences of exchanging tryptophan and tyrosine residues in B.stearothermophilus lactate dehydrogenase

scientific article published on 01 October 1992

Understanding the mechanism of ice binding by type III antifreeze proteins

scientific article

Use of a codon alteration strategy in a novel approach to cloning the Mycobacterium tuberculosis diaminopimelic acid epimerase

scientific journal article

Vancomycin resistance in enterococci: reprogramming of the D-ala-D-Ala ligases in bacterial peptidoglycan biosynthesis

scientific article

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