List of works - Tom Desmet

A constitutive expression system for high-throughput screening

A nitrilase-mediated entry to 4-carboxymethyl-β-lactams from chemically prepared 4-(cyanomethyl)azetidin-2-ones

article

A quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues in HjLPMO9A regioselectivity

scientific article published on 31 May 2017

A structural classification of carbohydrate epimerases: From mechanistic insights to practical applications

scientific article

Adsorption–desorption of trehalose analogues from a bioconversion mixture using activated carbon

An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity

scientific article published on 11 September 2012

An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina

scientific article published on January 2007

Analysis of the substrate specificity of α-L-arabinofuranosidases by DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis

scientific article published on 28 September 2018

Assessment of the trifluoromethyl ketone functionality as an alternative zinc-binding group for selective HDAC6 inhibition

scientific article published on 18 May 2018

Biocatalytic Synthesis of the Rare Sugar Kojibiose: Process Scale-Up and Application Testing

scientific article published on 30 June 2017

Biocatalytic production of novel glycolipids with cellodextrin phosphorylase

scientific article published on 25 September 2011

Biphasic Catalysis with Disaccharide Phosphorylases: Chemoenzymatic Synthesis of α-d-Glucosides Using Sucrose Phosphorylase

article

Broadening the synthetic potential of disaccharide phosphorylases through enzyme engineering

scientific article published in 2012

Characterization and mutational analysis of the UDP-Glc(NAc) 4-epimerase from Marinithermus hydrothermalis.

scientific article published on 19 December 2012

Characterization of the First Bacterial and Thermostable GDP-Mannose 3,5-Epimerase

scientific article published on 19 July 2019

Characterization of β-galactoside phosphorylases with diverging acceptor specificities

scientific article published on 7 April 2011

Chemoenzymatic Approach toward the Synthesis of 3-O-(α/β)-Glucosylated 3-Hydroxy-β-lactams

scientific article published on 12 November 2018

Chemoenzymatic Synthesis of β-D -Glucosides using Cellobiose Phosphorylase from Clostridium thermocellum

article

Chemoenzymatic synthesis of α-L-rhamnosides using recombinant α-L-rhamnosidase from Aspergillus terreus.

scientific article published on 23 August 2013

Collision cross section prediction of deprotonated phenolics in a travelling-wave ion mobility spectrometer using molecular descriptors and chemometrics

scientific article published on 25 April 2016

Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input

scientific article published on 7 May 2013

Construction of cellobiose phosphorylase variants with broadened acceptor specificity towards anomerically substituted glucosides

scientific article published in October 2010

Converting Galactose into the Rare Sugar Talose with Cellobiose 2-Epimerase as Biocatalyst

scientific article published in October 2018

Converting bulk sugars into prebiotics: semi-rational design of a transglucosylase with controlled selectivity

scientific article published on 9 February 2016

CorNet: Assigning function to networks of co-evolving residues by automated literature mining

scientific article

Correlated positions in protein evolution and engineering

scientific article

Corrigendum to ‘Characterization of β-galactoside phosphorylases with diverging acceptor specificities’ [Enzyme Microb. Technol. 49 (2012) 59–65]

scholarly article published in Enzyme and Microbial Technology

Creating Space for Large Acceptors: Rational Biocatalyst Design for Resveratrol Glycosylation in an Aqueous System

scientific article published on 12 June 2015

Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase

scientific article

Crystal complex structures reveal how substrate is bound in the -4 to the +2 binding sites of Humicola grisea Cel12A.

scientific article

Crystallization and X-ray diffraction studies of cellobiose phosphorylase from Cellulomonas uda.

scientific article

Development and application of a screening assay for glycoside phosphorylases

scientific article

Diastereoselective synthesis of 3-acetoxy-4-(3-aryloxiran-2-yl)azetidin-2-ones and their transformation into 3,4-oxolane-fused bicyclic β-lactams

scientific article published on 17 November 2016

Disulfide bridges as essential elements for the thermostability of lytic polysaccharide monooxygenase LPMO10C from Streptomyces coelicolor

scientific article

Engineering of cellobiose phosphorylase for glycoside synthesis

scientific article published on December 2010

Engineering the acceptor specificity of trehalose phosphorylase for the production of trehalose analogs

scientific article

Engineering the carbohydrate-binding site of Epa1p from Candida glabrata: generation of adhesin mutants with different carbohydrate specificity

scientific article published on 21 July 2014

Engineering the specificity of trehalose phosphorylase as a general strategy for the production of glycosyl phosphates

scientific article

Enzymatic Glycosylation of Phenolic Antioxidants: Phosphorylase-Mediated Synthesis and Characterization

scientific article published on 5 November 2015

Enzymatic glycosyl transfer: mechanisms and applications

Enzymatic glycosylation of small molecules: challenging substrates require tailored catalysts

scientific article published on 08 August 2012

Enzymatic production of alpha-D-galactose 1-phosphate by lactose phosphorolysis.

scientific article published on 24 July 2009

Enzymatic production of β-D-glucose-1-phosphate from trehalose

scientific article published on September 2010

Enzymatic properties and substrate specificity of the trehalose phosphorylase from Caldanaerobacter subterraneus

scientific article published on 29 July 2011

Enzymes for the biocatalytic production of rare sugars

scientific article published on 14 February 2012

Evaluation of (4-aminobutyloxy)quinolines as a novel class of antifungal agents

scientific article published on 13 June 2013

Evaluation of automated nano-electrospray mass spectrometry in the determination of non-covalent protein-ligand complexes

scientific article

Exploring the sequence diversity in glycoside hydrolase family 13_18 reveals a novel glucosylglycerol phosphorylase.

scientific article published on 22 February 2018

Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism

scientific article published on 28 July 2017

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP.

scientific article

High-yield synthesis of 2-O-α-d-glucosyl-d-glycerate by a bifunctional glycoside phosphorylase

scientific article published in 2024

Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes.

scientific article published on 7 April 2015

Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis

scientific article published on 6 September 2011

Increasing the thermostability of sucrose phosphorylase by multipoint covalent immobilization

scientific article published on 4 August 2010

Ionic liquids as cosolvents for glycosylation by sucrose phosphorylase: balancing acceptor solubility and enzyme stability

article

Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning

Modulation of activity by Arg407: structure of a fungal α-1,2-mannosidase in complex with a substrate analogue

scientific article

Operational stability of immobilized sucrose phosphorylase: Continuous production of α-glucose-1-phosphate at elevated temperatures

article

Potent and selective HDAC6 inhibitory activity of N-(4-hydroxycarbamoylbenzyl)-1,2,4,9-tetrahydro-3-thia-9-azafluorenes as novel sulfur analogues of Tubastatin A.

scientific article published on 28 March 2013

Probing the active site of cellodextrin phosphorylase from Clostridium stercorarium: kinetic characterization, ligand docking, and site-directed mutagenesis

scientific article published on 22 February 2011

Rational design of an improved transglucosylase for production of the rare sugar nigerose

scientific article published on 01 April 2019

Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing

scientific article published on 19 August 2015

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1.

scientific article published in July 2016

Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

scientific article published in September 2016

Structural Comparison of a Promiscuous and a Highly Specific Sucrose 6F-Phosphate Phosphorylase

scientific article published on 11 August 2019

Structural Features on the Substrate-Binding Surface of Fungal Lytic Polysaccharide Monooxygenases Determine Their Oxidative Regioselectivity

scientific article published on 20 September 2018

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

scientific article published on 05 April 2020

Sucrose phosphorylase as cross-linked enzyme aggregate: improved thermal stability for industrial applications

scientific article published on 24 September 2010

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

scientific article

Synthesis and Antimicrobial/Cytotoxic Assessment of Ferrocenyl Oxazinanes, Oxazinan-2-ones, and Tetrahydropyrimidin-2-ones

Synthesis and Applications of 3-Methylene-4-(trifluoromethyl)azetidin-2-ones as Building Blocks for the Preparation of Mono- and Spirocyclic 4-CF3 -β-Lactams

Synthesis and Evaluation of 2-Deoxy-2-amino-β-cellobiosides as Cellulase Inhibitors

Synthesis and SAR assessment of novel Tubathian analogs in the pursuit of potent and selective HDAC6 inhibitors

scientific article published on 29 January 2016

Synthesis of 2-aminomethyl-4-phenyl-1-azabicyclo[2.2.1]heptanes via LiAlH₄-induced reductive cyclization of 2-(4-chloro-2-cyano-2-phenylbutyl)aziridines and evaluation of their antimalarial activity

scientific article published on 20 December 2012

Synthesis of 2-aryl-3-(2-cyanoethyl)aziridines and their chemical and enzymatic hydrolysis towards γ-lactams and γ-lactones

scientific article published in March 2015

Synthesis of Non-Symmetrical Nitrogen-Containing Curcuminoids in the Pursuit of New Anticancer Candidates

scientific article published on 27 February 2019

Synthesis of Novel Aza-aromatic Curcuminoids with Improved Biological Activities towards Various Cancer Cell Lines.

scientific article

Synthesis of Potent and Selective HDAC6 Inhibitors Bearing a Cyclohexane- or Cycloheptane-Annulated 1,5-Benzothiazepine Scaffold

scientific article published on 7 October 2016

Synthesis of benzothiophene-based hydroxamic acids as potent and selective HDAC6 inhibitors

scientific article published on June 2015

Synthesis of functionalized 3-, 5-, 6- and 8-aminoquinolines via intermediate (3-pyrrolin-1-yl)- and (2-oxopyrrolidin-1-yl)quinolines and evaluation of their antiplasmodial and antifungal activity

scientific article published on 12 December 2014

Synthesis of halogenated 4-quinolones and evaluation of their antiplasmodial activity

scientific article published on 4 January 2014

Synthesis of piperidin-4-ones starting from 2-(2-bromo-1,1-dimethylethyl)azetidines and 2-(2-mesyloxyethyl)azetidines through a ring expansion–oxidation protocol

Synthesis, trehalase hydrolytic resistance and inhibition properties of 4- and 6-substituted trehalose derivatives

scientific article published on 01 December 2020

The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-phosphate phosphorylase as a novel specificity

scientific article published on 6 March 2014

The “epimerring” highlights the potential of carbohydrate epimerases for rare sugar production

article by Koen Beerens et al published 27 March 2017 in Biocatalysis & Biotransformation

Thermostable alpha-glucan phosphorylases: characteristics and industrial applications

scientific article published on 24 July 2018

Transglucosylation potential of six sucrose phosphorylases toward different classes of acceptors.

scientific article published on 24 June 2011

Trehalose Analogues: Latest Insights in Properties and Biocatalytic Production

scientific article published on 15 June 2015

UDP-hexose 4-epimerases: a view on structure, mechanism and substrate specificity

scientific article published on 21 June 2015

Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases

scientific article published on 22 October 2020

List of works by Tom Desmet

A constitutive expression system for high-throughput screening

A nitrilase-mediated entry to 4-carboxymethyl-β-lactams from chemically prepared 4-(cyanomethyl)azetidin-2-ones

A quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues in HjLPMO9A regioselectivity

A structural classification of carbohydrate epimerases: From mechanistic insights to practical applications

Adsorption–desorption of trehalose analogues from a bioconversion mixture using activated carbon

An imprinted cross-linked enzyme aggregate (iCLEA) of sucrose phosphorylase: combining improved stability with altered specificity

An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina

Analysis of the substrate specificity of α-L-arabinofuranosidases by DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis

Assessment of the trifluoromethyl ketone functionality as an alternative zinc-binding group for selective HDAC6 inhibition

Biocatalytic Synthesis of the Rare Sugar Kojibiose: Process Scale-Up and Application Testing

Biocatalytic production of novel glycolipids with cellodextrin phosphorylase

Biphasic Catalysis with Disaccharide Phosphorylases: Chemoenzymatic Synthesis of α-d-Glucosides Using Sucrose Phosphorylase

Broadening the synthetic potential of disaccharide phosphorylases through enzyme engineering

Characterization and mutational analysis of the UDP-Glc(NAc) 4-epimerase from Marinithermus hydrothermalis.

Characterization of the First Bacterial and Thermostable GDP-Mannose 3,5-Epimerase

Characterization of β-galactoside phosphorylases with diverging acceptor specificities

Chemoenzymatic Approach toward the Synthesis of 3-O-(α/β)-Glucosylated 3-Hydroxy-β-lactams

Chemoenzymatic Synthesis of β-D -Glucosides using Cellobiose Phosphorylase from Clostridium thermocellum

Chemoenzymatic synthesis of α-L-rhamnosides using recombinant α-L-rhamnosidase from Aspergillus terreus.

Collision cross section prediction of deprotonated phenolics in a travelling-wave ion mobility spectrometer using molecular descriptors and chemometrics

Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input

Construction of cellobiose phosphorylase variants with broadened acceptor specificity towards anomerically substituted glucosides

Converting Galactose into the Rare Sugar Talose with Cellobiose 2-Epimerase as Biocatalyst

Converting bulk sugars into prebiotics: semi-rational design of a transglucosylase with controlled selectivity

CorNet: Assigning function to networks of co-evolving residues by automated literature mining

Correlated positions in protein evolution and engineering

Corrigendum to ‘Characterization of β-galactoside phosphorylases with diverging acceptor specificities’ [Enzyme Microb. Technol. 49 (2012) 59–65]

Creating Space for Large Acceptors: Rational Biocatalyst Design for Resveratrol Glycosylation in an Aqueous System

Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase

Crystal complex structures reveal how substrate is bound in the -4 to the +2 binding sites of Humicola grisea Cel12A.

Crystallization and X-ray diffraction studies of cellobiose phosphorylase from Cellulomonas uda.

Development and application of a screening assay for glycoside phosphorylases

Diastereoselective synthesis of 3-acetoxy-4-(3-aryloxiran-2-yl)azetidin-2-ones and their transformation into 3,4-oxolane-fused bicyclic β-lactams

Disulfide bridges as essential elements for the thermostability of lytic polysaccharide monooxygenase LPMO10C from Streptomyces coelicolor

Engineering of cellobiose phosphorylase for glycoside synthesis

Engineering the acceptor specificity of trehalose phosphorylase for the production of trehalose analogs

Engineering the carbohydrate-binding site of Epa1p from Candida glabrata: generation of adhesin mutants with different carbohydrate specificity

Engineering the specificity of trehalose phosphorylase as a general strategy for the production of glycosyl phosphates

Enzymatic Glycosylation of Phenolic Antioxidants: Phosphorylase-Mediated Synthesis and Characterization

Enzymatic glycosyl transfer: mechanisms and applications

Enzymatic glycosylation of small molecules: challenging substrates require tailored catalysts

Enzymatic production of alpha-D-galactose 1-phosphate by lactose phosphorolysis.

Enzymatic production of β-D-glucose-1-phosphate from trehalose

Enzymatic properties and substrate specificity of the trehalose phosphorylase from Caldanaerobacter subterraneus

Enzymes for the biocatalytic production of rare sugars

Evaluation of (4-aminobutyloxy)quinolines as a novel class of antifungal agents

Evaluation of automated nano-electrospray mass spectrometry in the determination of non-covalent protein-ligand complexes

Exploring the sequence diversity in glycoside hydrolase family 13_18 reveals a novel glucosylglycerol phosphorylase.

Glucosylglycerate Phosphorylase, an Enzyme with Novel Specificity Involved in Compatible Solute Metabolism

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP.

High-yield synthesis of 2-O-α-d-glucosyl-d-glycerate by a bifunctional glycoside phosphorylase

Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes.

Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis

Increasing the thermostability of sucrose phosphorylase by multipoint covalent immobilization

Ionic liquids as cosolvents for glycosylation by sucrose phosphorylase: balancing acceptor solubility and enzyme stability

Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning

Modulation of activity by Arg407: structure of a fungal α-1,2-mannosidase in complex with a substrate analogue

Operational stability of immobilized sucrose phosphorylase: Continuous production of α-glucose-1-phosphate at elevated temperatures

Potent and selective HDAC6 inhibitory activity of N-(4-hydroxycarbamoylbenzyl)-1,2,4,9-tetrahydro-3-thia-9-azafluorenes as novel sulfur analogues of Tubastatin A.

Probing the active site of cellodextrin phosphorylase from Clostridium stercorarium: kinetic characterization, ligand docking, and site-directed mutagenesis

Rational design of an improved transglucosylase for production of the rare sugar nigerose

Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1.

Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

Structural Comparison of a Promiscuous and a Highly Specific Sucrose 6F-Phosphate Phosphorylase

Structural Features on the Substrate-Binding Surface of Fungal Lytic Polysaccharide Monooxygenases Determine Their Oxidative Regioselectivity

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

Sucrose phosphorylase as cross-linked enzyme aggregate: improved thermal stability for industrial applications

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

Synthesis and Antimicrobial/Cytotoxic Assessment of Ferrocenyl Oxazinanes, Oxazinan-2-ones, and Tetrahydropyrimidin-2-ones

Synthesis and Applications of 3-Methylene-4-(trifluoromethyl)azetidin-2-ones as Building Blocks for the Preparation of Mono- and Spirocyclic 4-CF3 -β-Lactams

Synthesis and Evaluation of 2-Deoxy-2-amino-β-cellobiosides as Cellulase Inhibitors

Synthesis and SAR assessment of novel Tubathian analogs in the pursuit of potent and selective HDAC6 inhibitors

Synthesis of 2-aminomethyl-4-phenyl-1-azabicyclo[2.2.1]heptanes via LiAlH₄-induced reductive cyclization of 2-(4-chloro-2-cyano-2-phenylbutyl)aziridines and evaluation of their antimalarial activity

Synthesis of 2-aryl-3-(2-cyanoethyl)aziridines and their chemical and enzymatic hydrolysis towards γ-lactams and γ-lactones

Synthesis of Non-Symmetrical Nitrogen-Containing Curcuminoids in the Pursuit of New Anticancer Candidates

Synthesis of Novel Aza-aromatic Curcuminoids with Improved Biological Activities towards Various Cancer Cell Lines.

Synthesis of Potent and Selective HDAC6 Inhibitors Bearing a Cyclohexane- or Cycloheptane-Annulated 1,5-Benzothiazepine Scaffold

Synthesis of benzothiophene-based hydroxamic acids as potent and selective HDAC6 inhibitors