List of works - William P Sheffield

A covalently linked recombinant albumin dimer is more rapidly cleared in vivo than are wild-type and mutant C34A albumin.

scientific article published in February 2004

A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss.

scientific article published on 5 April 2018

A long-lasting, plasmin-activatable thrombin inhibitor aids clot lysis in vitro and does not promote bleeding in vivo.

scientific article published in May 2009

Addition of a sequence from alpha2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis

scientific article

An important role for the activation peptide domain in controlling factor IX levels in the blood of haemophilia B mice

scientific article published on 01 December 2005

Bacterial neuraminidase-mediated erythrocyte desialylation provokes cell surface aminophospholipid exposure

scientific article published on 17 February 2018

Blood group biochemistry: a canadian blood services research and development symposium

scientific article published on 01 October 2005

Blood-Borne Pathogens: A Canadian Blood Services Centre for Innovation Symposium.

scientific article published on 23 February 2016

CD44 Antibody Inhibition of Macrophage Phagocytosis Targets Fcγ Receptor- and Complement Receptor 3-Dependent Mechanisms.

scientific article published on 04 March 2016

Changes in coagulation factor activity and content of di(2-ethylhexyl)phthalate in frozen plasma units during refrigerated storage for up to five days after thawing

scientific article published on 24 August 2011

Characterization of a highly polymorphic trinucleotide short tandem repeat within the human antithrombin gene

scientific article published on 01 May 1994

Characterization of a long-acting recombinant human serum albumin-atrial natriuretic factor (ANF) expressed in Pichia pastoris

scientific article published on 30 January 2012

Comparison of methods for the purification of alpha-1 acid glycoprotein from human plasma

scientific article

Complete nucleotide sequence of the cDNA encoding rabbit coagulation factor VII.

scientific article published on January 1993

Concepts and techniques in molecular biology--an overview.

scientific article published on July 1997

Conversion to the buffy coat method and quality of frozen plasma derived from whole blood donations in Canada

scientific article published on 08 January 2010

Cytokeratin 18 is expressed on the hepatocyte plasma membrane surface and interacts with thrombin-antithrombin complexes

scientific article published on 01 November 1997

Deletion mutagenesis of heparin cofactor II: defining the minimum size of a thrombin inhibiting serpin.

scientific article published in May 1995

Detection of antibodies reacting with the antithetical duffy blood group antigens Fy(a) and Fy(b) using recombinant fusion proteins containing the duffy extracellular domain.

scientific article published on 20 November 2006

Early γ-irradiation and subsequent storage of red cells in SAG-M additive solution potentiate energy imbalance, microvesiculation and susceptibility to stress-induced apoptotic cell death

scientific article published on 5 April 2017

Effects of genetic fusion of factor IX to albumin on in vivo clearance in mice and rabbits.

scientific article published in August 2004

Engineering the serpin α1 -antitrypsin: A diversity of goals and techniques

scientific article published on 09 December 2019

Expression screening of bacterial libraries of recombinant alpha-1 proteinase inhibitor variants for candidates with thrombin inhibitory capacity.

scientific article

Extending the pre-processing holding time of whole blood beyond 48 h reduces coagulation FVIII activity and immunoglobulin G content of recovered plasma

scientific article published on 19 September 2018

Factor VIIa gets even bigger

scientific article published on 01 April 2008

Full or partial substitution of the reactive center loop of alpha-1-proteinase inhibitor by that of heparin cofactor II: P1 Arg is required for maximal thrombin inhibition.

scientific article

Genetic linkage studies in antithrombin-deficient kindreds using a highly polymorphic trinucleotide short tandem repeat (STR) within the human antithrombin gene

scientific article published on 01 June 1994

Impact of mutations at the P4 and P5 positions on the reaction of antithrombin with thrombin and elastase

scientific article published on 01 October 1997

In vivo clearance of alpha-1 acid glycoprotein is influenced by the extent of its N-linked glycosylation and by its interaction with the vessel wall.

scientific article published in April 2012

Incorporation of albumin fusion proteins into fibrin clots in vitro and in vivo: comparison of different fusion motifs recognized by factor XIIIa.

scientific article

Inhibition of thrombin by hirudin genetically fused to wild-type or mutant antithrombin

scientific article published on 01 December 1996

Lipoprotein enrichment in orange insoluble particulate matter reproducibly appearing in cryoprecipitate

scientific article published on 23 November 2010

Modulation of clearance of recombinant serum albumin by either glycosylation or truncation

scientific article published on 01 September 2000

Molecular cloning and functional expression of rabbit alpha2-antiplasmin.

scientific article

Mutation of any site of N-linked glycosylation accelerates the in vivo clearance of recombinant rabbit antithrombin.

scientific article published in August 2000

Phosphatidylserine externalization and procoagulant activation of erythrocytes induced by Pseudomonas aeruginosa virulence factor pyocyanin

scientific article published on 19 January 2016

Plasma and Plasma Protein Product Transfusion: A Canadian Blood Services Centre for Innovation Symposium.

scientific article published on 26 March 2015

Prolonged in vivo anticoagulant activity of a hirudin-albumin fusion protein secreted from Pichia pastoris.

scientific article

Prothrombin complex concentrates reduce blood loss in murine coagulopathy induced by warfarin, but not in that induced by dabigatran etexilate.

scientific article published in September 2012

Prothrombin, alone or in complex concentrates or plasma, reduces bleeding in a mouse model of blood exchange-induced coagulopathy

scientific article published on 10 September 2019

Quality Assessment of Established and Emerging Blood Components for Transfusion

scientific article published on 14 December 2016

Quality and Safety of Blood Products

scientific article published on 29 December 2016

Reduction of thrombus size in murine models of thrombosis following administration of recombinant α1-proteinase inhibitor mutant proteins.

scientific article

Selection and characterization of a DNA aptamer inhibiting coagulation factor XIa.

scientific article

Site-directed mutagenesis of the P2 residue of human antithrombin

scientific article published on 01 February 1994

Stability of Thawed Apheresis Fresh-Frozen Plasma Stored for up to 120 Hours at 1°C to 6°C.

scientific article

Stability of relevant plasma protein activities in cryosupernatant plasma units during refrigerated storage for up to 5 days postthaw

scientific article

The M358R variant of α(1)-proteinase inhibitor inhibits coagulation factor VIIa.

scientific article

The appended tail region of heparin cofactor II and additional reactive centre loop mutations combine to increase the reactivity and specificity of alpha1-proteinase inhibitor M358R for thrombin

scientific article published on 01 November 2007

The complete N-terminal extension of heparin cofactor II is required for maximal effectiveness as a thrombin exosite 1 ligand

scientific article

The transferable tail: fusion of the N-terminal acidic extension of heparin cofactor II to alpha1-proteinase inhibitor M358R specifically increases the rate of thrombin inhibition

scientific article

Thromboelastometry reveals similar hemostatic properties of purified fibrinogen and a mixture of purified cryoprecipitate protein components

scientific article published on 19 February 2018

Thrombolysis by chemically modified coagulation factor Xa

scientific article published on 30 June 2016

γT -S195A thrombin reduces the anticoagulant effects of dabigatran in vitro and in vivo.

scientific article published on 19 June 2014

List of works by William P Sheffield

A covalently linked recombinant albumin dimer is more rapidly cleared in vivo than are wild-type and mutant C34A albumin.

A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss.

A long-lasting, plasmin-activatable thrombin inhibitor aids clot lysis in vitro and does not promote bleeding in vivo.

Addition of a sequence from alpha2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis

An important role for the activation peptide domain in controlling factor IX levels in the blood of haemophilia B mice

Bacterial neuraminidase-mediated erythrocyte desialylation provokes cell surface aminophospholipid exposure

Blood group biochemistry: a canadian blood services research and development symposium

Blood-Borne Pathogens: A Canadian Blood Services Centre for Innovation Symposium.

CD44 Antibody Inhibition of Macrophage Phagocytosis Targets Fcγ Receptor- and Complement Receptor 3-Dependent Mechanisms.

Changes in coagulation factor activity and content of di(2-ethylhexyl)phthalate in frozen plasma units during refrigerated storage for up to five days after thawing

Characterization of a highly polymorphic trinucleotide short tandem repeat within the human antithrombin gene

Characterization of a long-acting recombinant human serum albumin-atrial natriuretic factor (ANF) expressed in Pichia pastoris

Comparison of methods for the purification of alpha-1 acid glycoprotein from human plasma

Complete nucleotide sequence of the cDNA encoding rabbit coagulation factor VII.

Concepts and techniques in molecular biology--an overview.

Conversion to the buffy coat method and quality of frozen plasma derived from whole blood donations in Canada

Cytokeratin 18 is expressed on the hepatocyte plasma membrane surface and interacts with thrombin-antithrombin complexes

Deletion mutagenesis of heparin cofactor II: defining the minimum size of a thrombin inhibiting serpin.

Detection of antibodies reacting with the antithetical duffy blood group antigens Fy(a) and Fy(b) using recombinant fusion proteins containing the duffy extracellular domain.

Early γ-irradiation and subsequent storage of red cells in SAG-M additive solution potentiate energy imbalance, microvesiculation and susceptibility to stress-induced apoptotic cell death

Effects of genetic fusion of factor IX to albumin on in vivo clearance in mice and rabbits.

Engineering the serpin α1 -antitrypsin: A diversity of goals and techniques

Expression screening of bacterial libraries of recombinant alpha-1 proteinase inhibitor variants for candidates with thrombin inhibitory capacity.

Extending the pre-processing holding time of whole blood beyond 48 h reduces coagulation FVIII activity and immunoglobulin G content of recovered plasma

Factor VIIa gets even bigger

Full or partial substitution of the reactive center loop of alpha-1-proteinase inhibitor by that of heparin cofactor II: P1 Arg is required for maximal thrombin inhibition.

Genetic linkage studies in antithrombin-deficient kindreds using a highly polymorphic trinucleotide short tandem repeat (STR) within the human antithrombin gene

Impact of mutations at the P4 and P5 positions on the reaction of antithrombin with thrombin and elastase

In vivo clearance of alpha-1 acid glycoprotein is influenced by the extent of its N-linked glycosylation and by its interaction with the vessel wall.

Incorporation of albumin fusion proteins into fibrin clots in vitro and in vivo: comparison of different fusion motifs recognized by factor XIIIa.

Inhibition of thrombin by hirudin genetically fused to wild-type or mutant antithrombin

Lipoprotein enrichment in orange insoluble particulate matter reproducibly appearing in cryoprecipitate

Modulation of clearance of recombinant serum albumin by either glycosylation or truncation

Molecular cloning and functional expression of rabbit alpha2-antiplasmin.

Mutation of any site of N-linked glycosylation accelerates the in vivo clearance of recombinant rabbit antithrombin.

Phosphatidylserine externalization and procoagulant activation of erythrocytes induced by Pseudomonas aeruginosa virulence factor pyocyanin

Plasma and Plasma Protein Product Transfusion: A Canadian Blood Services Centre for Innovation Symposium.

Prolonged in vivo anticoagulant activity of a hirudin-albumin fusion protein secreted from Pichia pastoris.

Prothrombin complex concentrates reduce blood loss in murine coagulopathy induced by warfarin, but not in that induced by dabigatran etexilate.

Prothrombin, alone or in complex concentrates or plasma, reduces bleeding in a mouse model of blood exchange-induced coagulopathy

Quality Assessment of Established and Emerging Blood Components for Transfusion

Quality and Safety of Blood Products

Reduction of thrombus size in murine models of thrombosis following administration of recombinant α1-proteinase inhibitor mutant proteins.

Selection and characterization of a DNA aptamer inhibiting coagulation factor XIa.

Site-directed mutagenesis of the P2 residue of human antithrombin

Stability of Thawed Apheresis Fresh-Frozen Plasma Stored for up to 120 Hours at 1°C to 6°C.

Stability of relevant plasma protein activities in cryosupernatant plasma units during refrigerated storage for up to 5 days postthaw

The M358R variant of α(1)-proteinase inhibitor inhibits coagulation factor VIIa.

The appended tail region of heparin cofactor II and additional reactive centre loop mutations combine to increase the reactivity and specificity of alpha1-proteinase inhibitor M358R for thrombin

The complete N-terminal extension of heparin cofactor II is required for maximal effectiveness as a thrombin exosite 1 ligand

The transferable tail: fusion of the N-terminal acidic extension of heparin cofactor II to alpha1-proteinase inhibitor M358R specifically increases the rate of thrombin inhibition

Thromboelastometry reveals similar hemostatic properties of purified fibrinogen and a mixture of purified cryoprecipitate protein components

Thrombolysis by chemically modified coagulation factor Xa

γT -S195A thrombin reduces the anticoagulant effects of dabigatran in vitro and in vivo.