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List of works by Patrick M. Schaeffer

A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase.

scientific article

A molecular mousetrap determines polarity of termination of DNA replication in E. coli

scientific article

A new bivalent fluorescent fusion protein for differential Cu(II) and Zn(II) ion detection in aqueous solution

scientific article published on 10 December 2019

A polyplex qPCR-based binding assay for protein-DNA interactions.

scientific article published on 2 August 2012

A universal immuno-PCR platform for comparative and ultrasensitive quantification of dual affinity-tagged proteins in complex matrices.

scientific article published on 21 September 2012

Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein.

scientific article

Crystal and solution structures of the helicase-binding domain of Escherichia coli primase

scientific article

Defining specific allergens for improved component-resolved diagnosis of shrimp allergy in adults

scientific article published on 24 June 2019

Development of a protease activity assay using heat-sensitive Tus-GFP fusion protein substrates.

scientific article published on 27 April 2011

Differential Tus–Ter binding and lock formation: implications for DNA replication termination in Escherichia coli ⬇️

scientific article published on October 1, 2012

Dissecting the salt dependence of the Tus-Ter protein-DNA complexes by high-throughput differential scanning fluorimetry of a GFP-tagged Tus.

scientific article

ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis.

scientific article published on 21 November 2012

Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA)

scientific article published on 01 January 2020

Expression, purification, crystallization, and NMR studies of the helicase interaction domain of Escherichia coli DnaG primase

scientific article published in February 2004

Functional characterisation of Burkholderia pseudomallei biotin protein ligase: A toolkit for anti-melioidosis drug development.

scientific article

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis

scientific article

High-Throughput Differential Scanning Fluorimetry of GFP-Tagged Proteins

scientific article published on 01 January 2020

IgE reactivity to shrimp allergens in infants and their cross-reactivity to house dust mite

scientific article published on 7 August 2017

IgG-detection devices for the Tus-Ter-lock immuno-PCR diagnostic platform.

scientific article

Improved diagnosis of melioidosis using a 2-dimensional immunoarray

scientific article

Integron-associated mobile gene cassettes code for folded proteins: the structure of Bal32a, a new member of the adaptable alpha+beta barrel family.

scientific article

Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: insights into substrate recognition and the mechanism of catalysis

scientific article

Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase.

scientific article

Multiple oligomeric forms of Escherichia coli DnaB helicase revealed by electrospray ionisation mass spectrometry.

scientific article

Negative regulators of cell death pathways in cancer: perspective on biomarkers and targeted therapies.

scientific article published on 10 January 2018

Optimization of an Escherichia coli system for cell-free synthesis of selectively N-labelled proteins for rapid analysis by NMR spectroscopy

scientific article published in October 2004

Protein--protein interactions in the eubacterial replisome

scientific article

Proteomic dissection of DNA polymerization

scientific article

Quantitative determination of protein stability and ligand binding using a green fluorescent protein reporter system

scientific article

Selective protein unfolding: a universal mechanism of action for the development of irreversible inhibitors

scientific article

Single-molecule studies of fork dynamics in Escherichia coli DNA replication

scientific article published on 27 January 2008

Single-molecule studies of fork dynamics in Escherichia coli DNA replication

scientific article published on 01 September 2008

Site-specific covalent attachment of DNA to proteins using a photoactivatable Tus-Ter complex

scientific article

Tus-Ter-lock immuno-PCR assays for the sensitive detection of tropomyosin-specific IgE antibodies

scientific article published on 01 February 2014

Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system

scientific article published in July 2010

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