List of works - Juan A Hermoso

A complement to the modern crystallographer's toolbox: caged gadolinium complexes with versatile binding modes

scientific article

A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution

scientific article

Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa

scientific article

Allostery, Recognition of Nascent Peptidoglycan, and Cross-linking of the Cell Wall by the Essential Penicillin-Binding Protein 2x of Streptococcus pneumoniae

scientific article published on 22 January 2018

C-terminal tyrosine of ferredoxin-NADP+ reductase in hydride transfer processes with NAD(P)+/H.

scientific article

Carbohydrate recognition and lysis by bacterial peptidoglycan hydrolases.

scientific article published on 19 January 2017

Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa and Its Implications for Biofilm Formation

scientific article published on 07 January 2020

Catalytic Cycle of the N-Acetylglucosaminidase NagZ from Pseudomonas aeruginosa.

scientific article

Cell-Wall Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas aeruginosa

scientific article

Characterization of gadolinium complexes for SAD phasing in macromolecular crystallography: application to CbpF.

scientific article published on 17 July 2009

Chemical modification of protein surfaces to improve their reversible enzyme immobilization on ionic exchangers

scientific article published on 01 November 2006

Class A PBPs have a distinct and unique role in the construction of the pneumococcal cell wall

scientific article published on 02 March 2020

Cold-induced aldimine bond cleavage by Tris in Bacillus subtilis alanine racemase

scientific article published on 01 April 2019

Common conformational changes in flavodoxins induced by FMN and anion binding: the structure of Helicobacter pylori apoflavodoxin

scientific article

Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain

scientific article

Conformational Dynamics in Penicillin-Binding Protein 2a of Methicillin-Resistant Staphylococcus aureus, Allosteric Communication Network and Enablement of Catalysis.

scientific article published on 18 January 2017

Critical role of micelles in pancreatic lipase activation revealed by small angle neutron scattering

scientific article published on 01 February 2000

Crystal Structures of Bacterial Peptidoglycan Amidase AmpD and an Unprecedented Activation Mechanism

scientific article

Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation

scientific article

Crystal structure of CbpF, a bifunctional choline-binding protein and autolysis regulator from Streptococcus pneumoniae

scientific article

Crystal structure of CbpF, a bifunctional choline-binding protein and autolysis regulator from Streptococcus pneumoniae

scientific article published on 13 March 2009

Crystal structure of β-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases ⬇️

scientific article published on January 23, 1998

Crystal structures of CbpF complexed with atropine and ipratropium reveal clues for the design of novel antimicrobials against Streptococcus pneumoniae

scientific article

Crystal structures of Ophiostoma piceae sterol esterase: structural insights into activation mechanism and product release

scientific article

Crystal structures of methionine adenosyltransferase complexed with substrates and products reveal the methionine-ATP recognition and give insights into the catalytic mechanism

scientific article

Crystallization and preliminary X-ray diffraction analysis of ferredoxin-NADP(H) reductase from Rhodobacter capsulatus.

scientific article published on 26 November 2004

Crystallization and preliminary X-ray diffraction analysis of phosphoglycerate kinase from Streptococcus pneumoniae.

scientific article published on 30 September 2011

Crystallization and preliminary X-ray diffraction analysis of the lytic transglycosylase MltE from Escherichia coli ⬇️

scientific article published on December 24, 2010

Crystallization and preliminary X-ray diffraction studies of FAD synthetase from Corynebacterium ammoniagenes

scientific article published on 27 November 2009

Crystallization and preliminary X-ray diffraction studies of choline-binding protein F from Streptococcus pneumoniae

scientific article

Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus.

scientific article published on 31 October 2008

Crystallization and preliminary X-ray diffraction studies of the complete modular endolysin from Cp-1, a phage infecting Streptococcus pneumoniae

scientific article published on 23 August 2002

Crystallization and preliminary X-ray diffraction studies of the pneumococcal teichoic acid phosphorylcholine esterase Pce.

scientific article published in February 2005

Crystallization and preliminary X-ray diffraction studies of the transcriptional repressor PaaX, the main regulator of the phenylacetic acid degradation pathway in Escherichia coli W.

scientific article published on 30 September 2011

Crystallization and preliminary X-ray diffraction studies of the water-soluble state of the pore-forming toxin sticholysin II from the sea anemone Stichodactyla helianthus

scientific article published on 20 June 2002

Crystallization and preliminary X-ray diffraction studies of two different crystal forms of the lipase 2 isoform from the yeast Candida rugosa

scientific article published on 21 February 2003

Crystallization and preliminary crystallographic analysis of a novel haemolytic lectin from the mushroom Laetiporus sulphureus.

scientific article

Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus.

scientific article published on 05 April 2008

Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan

scientific article published on 28 November 2017

Discovery of antibiotic (E)-3-(3-carboxyphenyl)-2-(4-cyanostyryl)quinazolin-4(3H)-one

scientific article

Discovery of specific flavodoxin inhibitors as potential therapeutic agents against Helicobacter pylori infection

scientific article

Disruption of Allosteric Response as an Unprecedented Mechanism of Resistance to Antibiotics

scientific article

Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1

scientific article

Enhancement of the stability of a prolipase from Rhizopus oryzae toward aldehydes by saturation mutagenesis

scientific article

Flavodoxin: a compromise between efficiency and versatility in the electron transfer from Photosystem I to Ferredoxin-NADP(+) reductase

scientific article

Genetic modification of the penicillin G acylase surface to improve its reversible immobilization on ionic exchangers.

scientific article published on 10 November 2006

Getting CAD in shape: the atomic structure of human dihydroorotase domain

scientific article published on 01 February 2014

Heteroresistance to Fosfomycin Is Predominant in Streptococcus pneumoniae and Depends on the murA1 Gene

scientific article

High-Resolution Crystal Structure of MltE, an Outer Membrane-Anchored Endolytic Peptidoglycan Lytic Transglycosylase from Escherichia coli

scientific article

How allosteric control of Staphylococcus aureus penicillin binding protein 2a enables methicillin resistance and physiological function

scientific article

Influence of the conformational flexibility on the kinetics and dimerisation process of two Candida rugosa lipase isoenzymes.

scientific article published in July 2001

Insights into Molecular Plasticity of Choline Binding Proteins (Pneumococcal Surface Proteins) by SAXS

article by Rubén M. Buey et al published January 2007 in Journal of Molecular Biology

Insights into pneumococcal fratricide from the crystal structures of the modular killing factor LytC

scientific article

Insights into pneumococcal pathogenesis from the crystal structure of the modular teichoic acid phosphorylcholine esterase Pce.

scientific article published on 15 May 2005

Involvement of the pyrophosphate and the 2'-phosphate binding regions of ferredoxin-NADP+ reductase in coenzyme specificity

scientific article

Mechanism of the Escherichia coli MltE lytic transglycosylase, the cell-wall-penetrating enzyme for Type VI secretion system assembly.

scientific article published on 7 March 2018

Mixed ion exchange supports as useful ion exchangers for protein purification: purification of penicillin G acylase from Escherichia coli.

scientific article published in February 2007

Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis

scientific article

Neutron crystallographic evidence of lipase-colipase complex activation by a micelle.

scientific article published on September 1997

New Alkaloid Antibiotics That Target the DNA Topoisomerase I of Streptococcus pneumoniae ⬇️

scientific article published on December 17, 2010

Oligomeric state in the crystal structure of modular FAD synthetase provides insights into its sequential catalysis in prokaryotes

scientific article

Orthologous and Paralogous AmpD Peptidoglycan Amidases from Gram-Negative Bacteria.

scientific article published on 21 June 2016

Pancreatic Lipase-Related Protein Type 1: A Double Mutation Restores a Significant Lipase Activity ⬇️

scientific article published on May 19, 1998

Pancreatic lipase-related protein type I: a specialized lipase or an inactive enzyme.

scientific article

Penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus.

scientific article

Peptidoglycan remodeling by the coordinated action of multispecific enzymes

scientific article

Pneumococcal phosphoglycerate kinase interacts with plasminogen and its tissue activator

scientific article

Pneumococcal phosphorylcholine esterase, Pce, contains a metal binuclear center that is essential for substrate binding and catalysis

scientific article published on 31 October 2005

Pneumococcal surface proteins: when the whole is greater than the sum of its parts.

scientific article published on 15 June 2012

Probing the determinants of substrate specificity of a feruloyl esterase, AnFaeA, from Aspergillus niger

scientific article published in September 2005

Probing the role of glutamic acid 139 of Anabaena ferredoxin-NADP+ reductase in the interaction with substrates

scientific article

Promiscuous enantioselective (−)-γ-lactamase activity in the Pseudomonas fluorescens esterase I

scientific article published on 22 February 2012

Pyrrolopyrimidine vs Imidazole-Phenyl-Thiazole Scaffolds in Nonpeptidic Dimerization Inhibitors of Leishmania infantum Trypanothione Reductase

scientific article published on 23 April 2019

Reaction Products and the X-ray Structure of AmpDh2, a Virulence Determinant of Pseudomonas aeruginosa

scientific article

Renew or die: The molecular mechanisms of peptidoglycan recycling and antibiotic resistance in Gram-negative pathogens

scientific article published on 29 July 2016

Role of Arg100 and Arg264 from Anabaena PCC 7119 ferredoxin-NADP+ reductase for optimal NADP+ binding and electron transfer

scientific article

Semisynthetic peptide-lipase conjugates for improved biotransformations

scientific article published on 02 August 2012

Stabilization of penicillin G acylase from Escherichia coli: site-directed mutagenesis of the protein surface to increase multipoint covalent attachment.

scientific article published on February 2004

Structural Characterization of the Essential Cell Division Protein FtsE and Its Interaction with FtsX in Streptococcus pneumoniae

scientific article published on 01 September 2020

Structural analysis of interactions for complex formation between Ferredoxin-NADP+ reductase and its protein partners

scientific article published on 01 May 2005

Structural analysis of the Laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars

scientific article

Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins

scientific article

Structural basis for membrane anchorage of viral phi29 DNA during replication

scientific article published on 07 November 2005

Structural basis for selective recognition of endogenous and microbial polysaccharides by macrophage receptor SIGN-R1

scientific article

Structural basis for selective recognition of pneumococcal cell wall by modular endolysin from phage Cp-1

scientific article

Structural basis for the broad specificity of a new family of amino-acid racemases

scientific article

Structural basis of PcsB-mediated cell separation in Streptococcus pneumoniae

scientific article

Structural basis of increased resistance to thermal denaturation induced by single amino acid substitution in the sequence of beta-glucosidase A from Bacillus polymyxa

scientific article

Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40.

scientific article

Structural insights into the coenzyme mediated monomer-dimer transition of the pro-apoptotic apoptosis inducing factor

scientific article

Structural insights into the lipase/esterase behavior in the Candida rugosa lipases family: crystal structure of the lipase 2 isoenzyme at 1.97A resolution

scientific article

Structure of full-length human phenylalanine hydroxylase in complex with tetrahydrobiopterin

scientific article published on 22 May 2019

Structure of the Functional Domain of φ29 Replication Organizer

scientific article published in Journal of Biological Chemistry

Structure of the pneumococcal l,d-carboxypeptidase DacB and pathophysiological effects of disabled cell wall hydrolases DacA and DacB

scientific article published in September 2014

Structure solution with ARCIMBOLDO using fragments derived from distant homology models.

scientific article published on 6 September 2014

Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity.

scientific article published in June 2015

The 1.49 A resolution crystal structure of PsbQ from photosystem II of Spinacia oleracea reveals a PPII structure in the N-terminal region

scientific article published in July 2005

The C-terminal extension of bacterial flavodoxin-reductases: involvement in the hydride transfer mechanism from the coenzyme

scientific article

The crystal structure of feruloyl esterase A from Aspergillus niger suggests evolutive functional convergence in feruloyl esterase family

scientific article published in April 2004

The crystal structure of tetrameric methionine adenosyltransferase from rat liver reveals the methionine-binding site

scientific article published in July 2000

The ferredoxin-NADP(H) reductase from Rhodobacter capsulatus: molecular structure and catalytic mechanism

scientific article published in September 2005

The lipase/colipase complex is activated by a micelle: neutron crystallographic evidence

scientific article published on 01 June 1998

Three-dimensional structures of Lipoproteins from Streptococcus pneumoniae and Staphylococcus aureus.

scientific article published on 27 October 2017

Tuning of the FMN binding and oxido-reduction properties by neighboring side chains in Anabaena flavodoxin

scientific article

Turnover of Bacterial Cell Wall by SltB3, a Multidomain Lytic Transglycosylase of Pseudomonas aeruginosa

scientific article

X-ray Structure of Catenated Lytic Transglycosylase SltB1.

scientific article published on 13 November 2017

List of works by Juan A Hermoso

A complement to the modern crystallographer's toolbox: caged gadolinium complexes with versatile binding modes

A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution

Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa

Allostery, Recognition of Nascent Peptidoglycan, and Cross-linking of the Cell Wall by the Essential Penicillin-Binding Protein 2x of Streptococcus pneumoniae

C-terminal tyrosine of ferredoxin-NADP+ reductase in hydride transfer processes with NAD(P)+/H.

Carbohydrate recognition and lysis by bacterial peptidoglycan hydrolases.

Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa and Its Implications for Biofilm Formation

Catalytic Cycle of the N-Acetylglucosaminidase NagZ from Pseudomonas aeruginosa.

Cell-Wall Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas aeruginosa

Characterization of gadolinium complexes for SAD phasing in macromolecular crystallography: application to CbpF.

Chemical modification of protein surfaces to improve their reversible enzyme immobilization on ionic exchangers

Class A PBPs have a distinct and unique role in the construction of the pneumococcal cell wall

Cold-induced aldimine bond cleavage by Tris in Bacillus subtilis alanine racemase

Common conformational changes in flavodoxins induced by FMN and anion binding: the structure of Helicobacter pylori apoflavodoxin

Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain

Conformational Dynamics in Penicillin-Binding Protein 2a of Methicillin-Resistant Staphylococcus aureus, Allosteric Communication Network and Enablement of Catalysis.

Critical role of micelles in pancreatic lipase activation revealed by small angle neutron scattering

Crystal Structures of Bacterial Peptidoglycan Amidase AmpD and an Unprecedented Activation Mechanism

Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation

Crystal structure of CbpF, a bifunctional choline-binding protein and autolysis regulator from Streptococcus pneumoniae

Crystal structure of CbpF, a bifunctional choline-binding protein and autolysis regulator from Streptococcus pneumoniae

Crystal structure of β-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases ⬇️

Crystal structures of CbpF complexed with atropine and ipratropium reveal clues for the design of novel antimicrobials against Streptococcus pneumoniae

Crystal structures of Ophiostoma piceae sterol esterase: structural insights into activation mechanism and product release

Crystal structures of methionine adenosyltransferase complexed with substrates and products reveal the methionine-ATP recognition and give insights into the catalytic mechanism

Crystallization and preliminary X-ray diffraction analysis of ferredoxin-NADP(H) reductase from Rhodobacter capsulatus.

Crystallization and preliminary X-ray diffraction analysis of phosphoglycerate kinase from Streptococcus pneumoniae.

Crystallization and preliminary X-ray diffraction analysis of the lytic transglycosylase MltE from Escherichia coli ⬇️

Crystallization and preliminary X-ray diffraction studies of FAD synthetase from Corynebacterium ammoniagenes

Crystallization and preliminary X-ray diffraction studies of choline-binding protein F from Streptococcus pneumoniae

Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus.

Crystallization and preliminary X-ray diffraction studies of the complete modular endolysin from Cp-1, a phage infecting Streptococcus pneumoniae

Crystallization and preliminary X-ray diffraction studies of the pneumococcal teichoic acid phosphorylcholine esterase Pce.

Crystallization and preliminary X-ray diffraction studies of the transcriptional repressor PaaX, the main regulator of the phenylacetic acid degradation pathway in Escherichia coli W.

Crystallization and preliminary X-ray diffraction studies of the water-soluble state of the pore-forming toxin sticholysin II from the sea anemone Stichodactyla helianthus

Crystallization and preliminary X-ray diffraction studies of two different crystal forms of the lipase 2 isoform from the yeast Candida rugosa

Crystallization and preliminary crystallographic analysis of a novel haemolytic lectin from the mushroom Laetiporus sulphureus.

Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus.

Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan

Discovery of antibiotic (E)-3-(3-carboxyphenyl)-2-(4-cyanostyryl)quinazolin-4(3H)-one

Discovery of specific flavodoxin inhibitors as potential therapeutic agents against Helicobacter pylori infection

Disruption of Allosteric Response as an Unprecedented Mechanism of Resistance to Antibiotics

Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1

Enhancement of the stability of a prolipase from Rhizopus oryzae toward aldehydes by saturation mutagenesis

Flavodoxin: a compromise between efficiency and versatility in the electron transfer from Photosystem I to Ferredoxin-NADP(+) reductase

Genetic modification of the penicillin G acylase surface to improve its reversible immobilization on ionic exchangers.

Getting CAD in shape: the atomic structure of human dihydroorotase domain

Heteroresistance to Fosfomycin Is Predominant in Streptococcus pneumoniae and Depends on the murA1 Gene

High-Resolution Crystal Structure of MltE, an Outer Membrane-Anchored Endolytic Peptidoglycan Lytic Transglycosylase from Escherichia coli

How allosteric control of Staphylococcus aureus penicillin binding protein 2a enables methicillin resistance and physiological function

Influence of the conformational flexibility on the kinetics and dimerisation process of two Candida rugosa lipase isoenzymes.

Insights into Molecular Plasticity of Choline Binding Proteins (Pneumococcal Surface Proteins) by SAXS

Insights into pneumococcal fratricide from the crystal structures of the modular killing factor LytC

Insights into pneumococcal pathogenesis from the crystal structure of the modular teichoic acid phosphorylcholine esterase Pce.

Involvement of the pyrophosphate and the 2'-phosphate binding regions of ferredoxin-NADP+ reductase in coenzyme specificity

Mechanism of the Escherichia coli MltE lytic transglycosylase, the cell-wall-penetrating enzyme for Type VI secretion system assembly.

Mixed ion exchange supports as useful ion exchangers for protein purification: purification of penicillin G acylase from Escherichia coli.

Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis

Neutron crystallographic evidence of lipase-colipase complex activation by a micelle.

New Alkaloid Antibiotics That Target the DNA Topoisomerase I of Streptococcus pneumoniae ⬇️

Oligomeric state in the crystal structure of modular FAD synthetase provides insights into its sequential catalysis in prokaryotes

Orthologous and Paralogous AmpD Peptidoglycan Amidases from Gram-Negative Bacteria.

Pancreatic Lipase-Related Protein Type 1: A Double Mutation Restores a Significant Lipase Activity ⬇️

Pancreatic lipase-related protein type I: a specialized lipase or an inactive enzyme.

Penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus.

Peptidoglycan remodeling by the coordinated action of multispecific enzymes

Pneumococcal phosphoglycerate kinase interacts with plasminogen and its tissue activator

Pneumococcal phosphorylcholine esterase, Pce, contains a metal binuclear center that is essential for substrate binding and catalysis

Pneumococcal surface proteins: when the whole is greater than the sum of its parts.

Probing the determinants of substrate specificity of a feruloyl esterase, AnFaeA, from Aspergillus niger

Probing the role of glutamic acid 139 of Anabaena ferredoxin-NADP+ reductase in the interaction with substrates

Promiscuous enantioselective (−)-γ-lactamase activity in the Pseudomonas fluorescens esterase I

Pyrrolopyrimidine vs Imidazole-Phenyl-Thiazole Scaffolds in Nonpeptidic Dimerization Inhibitors of Leishmania infantum Trypanothione Reductase

Reaction Products and the X-ray Structure of AmpDh2, a Virulence Determinant of Pseudomonas aeruginosa

Renew or die: The molecular mechanisms of peptidoglycan recycling and antibiotic resistance in Gram-negative pathogens

Role of Arg100 and Arg264 from Anabaena PCC 7119 ferredoxin-NADP+ reductase for optimal NADP+ binding and electron transfer

Semisynthetic peptide-lipase conjugates for improved biotransformations

Stabilization of penicillin G acylase from Escherichia coli: site-directed mutagenesis of the protein surface to increase multipoint covalent attachment.

Structural Characterization of the Essential Cell Division Protein FtsE and Its Interaction with FtsX in Streptococcus pneumoniae