List of works - Emily Flashman

Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure.

scientific article

Biochemical characterization of New Delhi metallo-β-lactamase variants reveals differences in protein stability.

scientific article

Cardiac myosin binding protein C: its role in physiology and disease

scientific article

Catalytic strategies of the non-heme iron dependent oxygenases and their roles in plant biology

scientific article

Cellular oxygen sensing: Crystal structure of hypoxia-inducible factor prolyl hydroxylase (PHD2)

scientific article

Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants

scientific article published on 01 July 2019

Critical role for IL-4 in the development of transplant arteriosclerosis in the absence of CD40-CD154 costimulation

scientific article published on 01 July 2001

Crystal structure of the C1 domain of cardiac myosin binding protein-C: implications for hypertrophic cardiomyopathy

scientific article

Direct analysis of enzyme-catalyzed DNA demethylation

scientific article

Dynamic states of the DNA repair enzyme AlkB regulate product release

scientific article published on 11 July 2008

ESI-MS studies on prolyl hydroxylase domain 2 reveal a new metal binding site

scientific article published in April 2008

Epigenetic regulation by histone demethylases in hypoxia

scientific article

Evidence for a stereoelectronic effect in human oxygen sensing.

scientific article

Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen

scientific article

Hypoxia and hypoxia mimetics differentially modulate histone post-translational modifications

scientific article published on 01 July 2020

Hypoxia-inducible factor prolyl hydroxylase 2 has a high affinity for ferrous iron and 2-oxoglutarate

scientific article

Identification of novel interactions between domains of Myosin binding protein-C that are modulated by hypertrophic cardiomyopathy missense mutations

scientific article

Investigating the contribution of the active site environment to the slow reaction of hypoxia-inducible factor prolyl hydroxylase domain 2 with oxygen

scientific article

Investigating the dependence of the hypoxia-inducible factor hydroxylases (factor inhibiting HIF and prolyl hydroxylase domain 2) on ascorbate and other reducing agents

scientific article

Investigations on the oxygen dependence of a 2-oxoglutarate histone demethylase.

scientific article published in January 2013

Kinetic Investigations of the Role of Factor Inhibiting Hypoxia-inducible Factor (FIH) as an Oxygen Sensor.

scientific article published on 25 June 2015

Kinetic rationale for selectivity toward N- and C-terminal oxygen-dependent degradation domain substrates mediated by a loop region of hypoxia-inducible factor prolyl hydroxylases

scientific article

Localization of the binding site of the C-terminal domain of cardiac myosin-binding protein-C on the myosin rod

scientific article

Lysine-241 has a role in coupling 2OG turnover with substrate oxidation during KDM4-catalysed histone demethylation

scientific article published on 14 February 2018

Molecular and cellular mechanisms of HIF prolyl hydroxylase inhibitors in clinical trials

scientific article published on 11 September 2017

Non-enzymatic chemistry enables 2-hydroxyglutarate-mediated activation of 2-oxoglutarate oxygenases.

scientific article

OGFOD1 catalyzes prolyl hydroxylation of RPS23 and is involved in translation control and stress granule formation

scientific article

OS-9: another piece in the HIF complex story

scientific article

Oxygen-sensing mechanisms across eukaryotic kingdoms and their roles in complex multicellularity

scientific article published on 01 October 2020

Plant cysteine oxidases are dioxygenases that directly enable arginyl transferase-catalysed arginylation of N-end rule targets

scientific article

Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication

scientific article

Secondary binding sites for triplex-forming oligonucleotides containing bulges, loops, and mismatches in the third strand.

scientific article published on June 2000

Small-molecules that covalently react with a human prolyl hydroxylase – towards activity modulation and substrate capture

scientific article published on 01 January 2019

Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases

scientific article

Structure and Mechanism of a Viral Collagen Prolyl Hydroxylase

scientific article

Structures of Arabidopsis thaliana oxygen-sensing plant cysteine oxidases 4 and 5 enable targeted manipulation of their activity

scientific article published on 31 August 2020

Studies on deacetoxycephalosporin C synthase support a consensus mechanism for 2-oxoglutarate dependent oxygenases.

scientific article

Studies on the Interaction of the Histone Demethylase KDM5B with Tricarboxylic Acid Cycle Intermediates.

scientific article published on 18 August 2017

Studies on the activity of the hypoxia-inducible-factor hydroxylases using an oxygen consumption assay

scientific article

Studies on the reaction of nitric oxide with the hypoxia-inducible factor prolyl hydroxylase domain 2 (EGLN1)

scientific article

Studying the active-site loop movement of the São Paolo metallo-β-lactamase-1†Electronic supplementary information (ESI) available: Procedures for protein expression and purification, 19F-labelling, crystallisation, data collection, and structure de

scientific article published on 4 November 2014

Support for a trimeric collar of myosin binding protein C in cardiac and fast skeletal muscle, but not in slow skeletal muscle

scientific article

The Activity of JmjC Histone Lysine Demethylase KDM4A is Highly Sensitive to Oxygen Concentrations

scientific article published on 4 January 2017

The most versatile of all reactive intermediates?

The plant cysteine oxidases from Arabidopsis thaliana are kinetically tailored to act as oxygen sensors

scientific article published on 30 May 2018

Use of ferrous iron by metallo-β-lactamases

scientific article

Use of mass spectrometry to probe the nucleophilicity of cysteinyl residues of prolyl hydroxylase domain 2.

scientific article

Using NMR solvent water relaxation to investigate metalloenzyme-ligand binding interactions

scientific article

YcfDRM is a thermophilic oxygen-dependent ribosomal protein uL16 oxygenase

scientific article published on 9 March 2018

List of works by Emily Flashman

Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure.

Biochemical characterization of New Delhi metallo-β-lactamase variants reveals differences in protein stability.

Cardiac myosin binding protein C: its role in physiology and disease

Catalytic strategies of the non-heme iron dependent oxygenases and their roles in plant biology

Cellular oxygen sensing: Crystal structure of hypoxia-inducible factor prolyl hydroxylase (PHD2)

Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants

Critical role for IL-4 in the development of transplant arteriosclerosis in the absence of CD40-CD154 costimulation

Crystal structure of the C1 domain of cardiac myosin binding protein-C: implications for hypertrophic cardiomyopathy

Direct analysis of enzyme-catalyzed DNA demethylation

Dynamic states of the DNA repair enzyme AlkB regulate product release

ESI-MS studies on prolyl hydroxylase domain 2 reveal a new metal binding site

Epigenetic regulation by histone demethylases in hypoxia

Evidence for a stereoelectronic effect in human oxygen sensing.

Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen

Hypoxia and hypoxia mimetics differentially modulate histone post-translational modifications

Hypoxia-inducible factor prolyl hydroxylase 2 has a high affinity for ferrous iron and 2-oxoglutarate

Identification of novel interactions between domains of Myosin binding protein-C that are modulated by hypertrophic cardiomyopathy missense mutations

Investigating the contribution of the active site environment to the slow reaction of hypoxia-inducible factor prolyl hydroxylase domain 2 with oxygen

Investigating the dependence of the hypoxia-inducible factor hydroxylases (factor inhibiting HIF and prolyl hydroxylase domain 2) on ascorbate and other reducing agents

Investigations on the oxygen dependence of a 2-oxoglutarate histone demethylase.

Kinetic Investigations of the Role of Factor Inhibiting Hypoxia-inducible Factor (FIH) as an Oxygen Sensor.

Kinetic rationale for selectivity toward N- and C-terminal oxygen-dependent degradation domain substrates mediated by a loop region of hypoxia-inducible factor prolyl hydroxylases

Localization of the binding site of the C-terminal domain of cardiac myosin-binding protein-C on the myosin rod

Lysine-241 has a role in coupling 2OG turnover with substrate oxidation during KDM4-catalysed histone demethylation

Molecular and cellular mechanisms of HIF prolyl hydroxylase inhibitors in clinical trials

Non-enzymatic chemistry enables 2-hydroxyglutarate-mediated activation of 2-oxoglutarate oxygenases.

OGFOD1 catalyzes prolyl hydroxylation of RPS23 and is involved in translation control and stress granule formation

OS-9: another piece in the HIF complex story

Oxygen-sensing mechanisms across eukaryotic kingdoms and their roles in complex multicellularity

Plant cysteine oxidases are dioxygenases that directly enable arginyl transferase-catalysed arginylation of N-end rule targets

Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication

Secondary binding sites for triplex-forming oligonucleotides containing bulges, loops, and mismatches in the third strand.

Small-molecules that covalently react with a human prolyl hydroxylase – towards activity modulation and substrate capture

Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases

Structure and Mechanism of a Viral Collagen Prolyl Hydroxylase

Structures of Arabidopsis thaliana oxygen-sensing plant cysteine oxidases 4 and 5 enable targeted manipulation of their activity

Studies on deacetoxycephalosporin C synthase support a consensus mechanism for 2-oxoglutarate dependent oxygenases.

Studies on the Interaction of the Histone Demethylase KDM5B with Tricarboxylic Acid Cycle Intermediates.

Studies on the activity of the hypoxia-inducible-factor hydroxylases using an oxygen consumption assay

Studies on the reaction of nitric oxide with the hypoxia-inducible factor prolyl hydroxylase domain 2 (EGLN1)

Studying the active-site loop movement of the São Paolo metallo-β-lactamase-1†Electronic supplementary information (ESI) available: Procedures for protein expression and purification, 19F-labelling, crystallisation, data collection, and structure de

Support for a trimeric collar of myosin binding protein C in cardiac and fast skeletal muscle, but not in slow skeletal muscle

The Activity of JmjC Histone Lysine Demethylase KDM4A is Highly Sensitive to Oxygen Concentrations

The most versatile of all reactive intermediates?

The plant cysteine oxidases from Arabidopsis thaliana are kinetically tailored to act as oxygen sensors

Use of ferrous iron by metallo-β-lactamases

Use of mass spectrometry to probe the nucleophilicity of cysteinyl residues of prolyl hydroxylase domain 2.

Using NMR solvent water relaxation to investigate metalloenzyme-ligand binding interactions

YcfDRM is a thermophilic oxygen-dependent ribosomal protein uL16 oxygenase