List of works - Harri Savilahti

A direct transposon insertion tool for modification and functional analysis of viral genomes.

scientific article

A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach

scientific article

A method to sequence and quantify DNA integration for monitoring outcome in gene therapy ⬇️

scientific article

A novel class of winged helix-turn-helix protein: the DNA-binding domain of Mu transposase

scientific article

A set of mini-Mu transposons for versatile cloning of circular DNA and novel dual-transposon strategy for increased efficiency

scientific article published on 4 July 2016

Actin-organising properties of the muscular dystrophy protein myotilin

scientific article

An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications.

scientific article published on July 1999

Application of Mu in vitro transposition for high-precision mapping of protein-protein interfaces on a yeast two-hybrid platform

scientific article

Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes

scientific article published in January 2018

Bacteriophage Mu integration in yeast and mammalian genomes

scientific article published on 25 October 2008

Bacteriophage PRD1 terminal protein: expression of gene VIII in Escherichia coli and purification of the functional P8 product

scientific article published in December 1989

Behavioural phenotypes of hypomorphic KCC2-deficient mice

scientific journal article

Characteristics of MuA transposase-catalyzed processing of model transposon end DNA hairpin substrates.

scientific article

Comparison of the amino acid sequence of the lytic enzyme from broad-host-range bacteriophage PRD1 with sequences of other cell-wall-peptidoglycan lytic enzymes.

scientific article published on March 1989

Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.

scientific article published in January 2001

Critical evaluation of random mutagenesis by error-prone polymerase chain reaction protocols, Escherichia coli mutator strain, and hydroxylamine treatment.

scientific article

DNA transposition of bacteriophage Mu. A quantitative analysis of target site selection in vitro

scientific article published on 07 November 2001

Division of labor among monomers within the Mu transposase tetramer

scientific article published on 01 August 1993

Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage mu

scientific article

Flexibility in MuA transposase family protein structures: functional mapping with scanning mutagenesis and sequence alignment of protein homologues

scientific article

Functional characterization of the human immunodeficiency virus type 1 genome by genetic footprinting

scientific article

Functional comparison of the transposition core machineries of phage Mu and Haemophilus influenzae Mu-like prophage Hin-Mu reveals interchangeable components.

scientific article

Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation.

scientific article

Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery.

scientific article

Generation of single-copy transposon insertions in Clostridium perfringens by electroporation of phage mu DNA transposition complexes

scientific article

Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes.

scientific article published in April 2005

Genetic recombination pathways and their application for genome modification of human embryonic stem cells

scientific article

Genome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis.

scientific article published on September 2006

High-precision mapping of protein protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform

scientific article

In vitro replication of bacteriophage PRD1 DNA. Metal activation of protein-primed initiation and DNA elongation ⬇️

scientific article published on August 11, 1992

Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus

scientific article

Linear DNA replication: inverted terminal repeats of five closely related Escherichia coli bacteriophages

scientific article published in January 1986

Meox1Cre: a mouse line expressing Cre recombinase in somitic mesoderm

scientific article published in November 2005

Missing-in-metastasis MIM/MTSS1 promotes actin assembly at intercellular junctions and is required for integrity of kidney epithelia

scientific journal article

Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1.

scientific article

Mu transposition complex mutagenesis in Lactococcus lactis--identification of genes affecting nisin production.

scientific article

Mu transpositional recombination: donor DNA cleavage and strand transfer in trans by the Mu transposase

scientific article published on 01 April 1996

Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.

scientific article published on 27 December 2017

MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.

scientific article published on 4 February 2016

Nonessential genes of phage phiYeO3-12 include genes involved in adaptation to growth on Yersinia enterocolitica serotype O:3.

scientific article

Phylogenetic analysis of Maverick/Polinton giant transposons across organisms

scientific article

Probing the alpha-complementing domain of E. coli beta-galactosidase with use of an insertional pentapeptide mutagenesis strategy based on Mu in vitro DNA transposition.

scientific article

Retrotransposon BARE-1: expression of encoded proteins and formation of virus-like particles in barley cells

scientific article published in November 1999

SNP discovery by mismatch-targeting of Mu transposition.

scientific article published on 20 February 2007

Structure-function analysis of PrsA reveals roles for the parvulin-like and flanking N- and C-terminal domains in protein folding and secretion in Bacillus subtilis

scientific article published on 19 February 2004

The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase

scientific article published in January 1987

The organization of the right-end early region of bacteriophage PRD1 genome.

scientific article

Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice

scientific article published on 05 February 2009

Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli

scientific article published on 2 December 2010

Universal platform for quantitative analysis of DNA transposition.

scientific article

List of works by Harri Savilahti

A direct transposon insertion tool for modification and functional analysis of viral genomes.

A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach

A method to sequence and quantify DNA integration for monitoring outcome in gene therapy ⬇️

A novel class of winged helix-turn-helix protein: the DNA-binding domain of Mu transposase

A set of mini-Mu transposons for versatile cloning of circular DNA and novel dual-transposon strategy for increased efficiency

Actin-organising properties of the muscular dystrophy protein myotilin

An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications.

Application of Mu in vitro transposition for high-precision mapping of protein-protein interfaces on a yeast two-hybrid platform

Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes

Bacteriophage Mu integration in yeast and mammalian genomes

Bacteriophage PRD1 terminal protein: expression of gene VIII in Escherichia coli and purification of the functional P8 product

Behavioural phenotypes of hypomorphic KCC2-deficient mice

Characteristics of MuA transposase-catalyzed processing of model transposon end DNA hairpin substrates.

Comparison of the amino acid sequence of the lytic enzyme from broad-host-range bacteriophage PRD1 with sequences of other cell-wall-peptidoglycan lytic enzymes.

Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.

Critical evaluation of random mutagenesis by error-prone polymerase chain reaction protocols, Escherichia coli mutator strain, and hydroxylamine treatment.

DNA transposition of bacteriophage Mu. A quantitative analysis of target site selection in vitro

Division of labor among monomers within the Mu transposase tetramer

Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage mu

Flexibility in MuA transposase family protein structures: functional mapping with scanning mutagenesis and sequence alignment of protein homologues

Functional characterization of the human immunodeficiency virus type 1 genome by genetic footprinting

Functional comparison of the transposition core machineries of phage Mu and Haemophilus influenzae Mu-like prophage Hin-Mu reveals interchangeable components.

Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation.

Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery.

Generation of single-copy transposon insertions in Clostridium perfringens by electroporation of phage mu DNA transposition complexes

Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes.

Genetic recombination pathways and their application for genome modification of human embryonic stem cells

Genome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis.

High-precision mapping of protein protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform

In vitro replication of bacteriophage PRD1 DNA. Metal activation of protein-primed initiation and DNA elongation ⬇️

Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus

Linear DNA replication: inverted terminal repeats of five closely related Escherichia coli bacteriophages

Meox1Cre: a mouse line expressing Cre recombinase in somitic mesoderm

Missing-in-metastasis MIM/MTSS1 promotes actin assembly at intercellular junctions and is required for integrity of kidney epithelia

Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1.

Mu transposition complex mutagenesis in Lactococcus lactis--identification of genes affecting nisin production.

Mu transpositional recombination: donor DNA cleavage and strand transfer in trans by the Mu transposase

Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.

MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.

Nonessential genes of phage phiYeO3-12 include genes involved in adaptation to growth on Yersinia enterocolitica serotype O:3.

Phylogenetic analysis of Maverick/Polinton giant transposons across organisms

Probing the alpha-complementing domain of E. coli beta-galactosidase with use of an insertional pentapeptide mutagenesis strategy based on Mu in vitro DNA transposition.

Retrotransposon BARE-1: expression of encoded proteins and formation of virus-like particles in barley cells

SNP discovery by mismatch-targeting of Mu transposition.

Structure-function analysis of PrsA reveals roles for the parvulin-like and flanking N- and C-terminal domains in protein folding and secretion in Bacillus subtilis

The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase

The organization of the right-end early region of bacteriophage PRD1 genome.

Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice

Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli

Universal platform for quantitative analysis of DNA transposition.