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List of works by Michael W Pfaffl

A method to assess 59Fe in residual tissue blood content in mice and its use to correct 59Fe-distribution kinetics accordingly

scientific article published on 07 August 2007

A new mathematical model for relative quantification in real-time RT-PCR

scientific article (publication date: May 2001)

Analysis of key molecules of the innate immune system in mammary epithelial cells isolated from marker-assisted and conventionally selected cattle.

scientific article

Angiogenesis in The Ovary - The Most Important Regulatory Event for Follicle and Corpus Luteum Development and Function in Cow - An Overview.

scientific article published on 6 May 2015

Blastocysts exhibit sex-specific signalling of IFNT transcription, translation and activity

scientific article published on 01 March 2019

Can milk cell or skim milk miRNAs be used as biomarkers for early pregnancy detection in cattle?

scientific article

Cellular and extracellular miRNAs are blood-compartment-specific diagnostic targets in sepsis.

scientific article published on 6 April 2017

Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy

scientific article published on 24 July 2018

Changes in the miRNA profile under the influence of anabolic steroids in bovine liver.

scientific article

Changes in the microRNA expression profile during blood storage

scientific article published on 05 July 2018

Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds.

scientific article published in February 2003

Class I odorant receptors, TAS1R and TAS2R taste receptors, are markers for subpopulations of circulating leukocytes

scientific article

Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research.

scientific article

Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR.

scientific article published on 10 August 2006

Comparison of reverse transcriptases in gene expression analysis.

scientific article

Comparison of the immune competence of Turopolje, German Landrace × Turopolje, and German Landrace × Pietrain pigs after PRRSV vaccination

scientific article

Comparison of the miRNome and piRNome of bovine blood and plasma by small RNA sequencing.

scientific article published on 21 February 2015

Detection and quantification of mRNA expression of alpha- and beta-adrenergic receptor subtypes in the mammary gland of dairy cows

scientific article published on 01 March 2003

Detection of Atherosclerosis by Small RNA-Sequencing Analysis of Extracellular Vesicle Enriched Serum Samples

scientific article published on 12 October 2021

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

scientific article (publication date: March 2004)

Development of clenbuterol reference materials: lyophilized bovine eye samples free of clenbuterol (CRM 673) and containing clenbuterol (CRM 674). Part 2: certification.

scientific article published in December 2001

Differential expression of molecular motors in the motor cortex of sporadic ALS

scientific article

Distribution-insensitive cluster analysis in SAS on real-time PCR gene expression data of steadily expressed genes.

scientific article

EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research.

scientific article published on February 2017

Editorial

scientific article published on 22 March 2019

Effect of Perilla frutescens Extracts on Porcine Jejunal Epithelial Cells.

scientific article published on 13 December 2016

Effect of insoluble fibre on intestinal morphology and mRNA expression pattern of inflammatory, cell cycle and growth marker genes in a piglet model.

scientific article published on December 2008

Effect of lactoferrin on selected immune system parameters and the gastrointestinal morphology in growing calves

scientific article published on 01 April 2007

Effect of lactulose on growth performance and intestinal morphology of pre-ruminant calves using a milk replacer containing Enterococcus faecium

scientific article published on March 2007

Effect of magnetic stimulation on the gene expression profile of in vitro cultured neural cells ⬇️

scientific article published on August 19, 2012

Effect of non-starch-polysaccharide-degrading enzymes as feed additive on the rumen bacterial population in non-lactating cows quantified by real-time PCR.

scientific article

Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells.

scientific article

Effect of trenbolone acetate plus estradiol on transcriptional regulation of metabolism pathways in bovine liver

scientific article published on 01 August 2010

Effects of inulin and lactulose on the intestinal morphology of calves

scientific article published on May 2010

Effects of lactoferrin feeding on growth, feed intake and health of calves

scientific article published on 01 February 2007

Effects of muscle type, castration, age, and compensatory growth rate on androgen receptor mRNA expression in bovine skeletal muscle.

scientific article published in March 2000

Effects of mycophenolic acid on inosine monophosphate dehydrogenase I and II mRNA expression in white blood cells and various tissues in sheep

scientific article published on 01 May 2006

Effects of synthetic progestagens on the mRNA expression of androgen receptor, progesterone receptor, oestrogen receptor alpha and beta, insulin-like growth factor-1 (IGF-1) and IGF-1 receptor in heifer tissues.

scientific article published in March 2002

Effects of the prebiotics inulin and lactulose on intestinal immunology and hematology of preruminant calves.

scientific article published on May 2011

Electric cell-substrate impedance sensing (ECIS) based real-time measurement of titer dependent cytotoxicity induced by adenoviral vectors in an IPI-2I cell culture model ⬇️

scientific article published on September 9, 2010

Elevated caspase-3 and Fas mRNA expression in jejunum of adult rats during subclinical zinc deficiency

scientific article published on 01 January 2004

Erratum: Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by Next-Generation Sequencing

scientific article published on 08 March 2019

Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk.

scientific article published on 22 June 2017

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications

scientific article

Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing.

scientific article

Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland during development, function and involution ⬇️

scientific article (publication date: May 2003)

Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.

scientific article published on 11 July 2016

Expression of estrogen and progesterone receptors in the bovine ovary during estrous cycle and pregnancy.

scientific article published in April 2002

Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary.

scientific article

Gene expression profiling in pbMEC - in search of molecular biomarkers to predict immunoglobulin production in bovine milk

scientific article published on 29 November 2017

Glucocorticoid receptor overexpression slightly shifts microRNA expression patterns in triple-negative breast cancer.

scientific article published on 27 March 2018

Grass pollen production and group V allergen content of agriculturally relevant species and cultivars

scientific article published on 12 March 2018

Growth Hormone Secretion Patterns in German Landrace (DL) Fetuses and Piglets Compared to DL Piglets with Inherited 1,25-Dihydroxyvitamin D3 Deficiency.

scientific article published on 15 May 2018

Guest editor's introduction for BDQ special issue: 'Advanced Molecular Diagnostics for Biomarker Discovery'.

scientific article

Highlights of the São Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication.

scientific article published on 26 November 2017

Highlights of the mini-symposium on extracellular vesicles in inter-organismal communication, held in Munich, Germany, August 2018

article

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.

scientific article

Human airway epithelial extracellular vesicle miRNA signature is altered upon asthma development

scientific article published on 02 October 2019

Identification of a piscine reovirus-related pathogen in proliferative darkening syndrome (PDS) infected brown trout (Salmo trutta fario) using a next-generation technology detection pipeline

scientific article published in PLoS ONE

Identification of a potential gene expression biomarker signature in bovine liver to detect the abuse of growth promoters.

scientific article

Identification of potential gene expression biomarkers for the surveillance of anabolic agents in bovine blood cells.

scientific article

Impact of elevated air temperature and drought on pollen characteristics of major agricultural grass species

scientific article published on 26 March 2021

Impact of preimplantational oral low-dose estradiol-17β exposure on the endometrium: the role of miRNA.

scientific article published on 15 March 2018

Influence of anabolic combinations of an androgen plus an estrogen on biochemical pathways in bovine uterine endometrium and ovary.

scientific article published on 25 January 2011

Influence of oral application of mycophenolic acid on the clinical health status of sheep

scientific article published on 01 March 2007

Influence of testosterone and a novel SARM on gene expression in whole blood of Macaca fascicularis.

scientific article

Influence of zinc deficiency on the mRNA expression of zinc transporters in adult rats.

scientific article published in January 2003

Inhibition of fat cell differentiation in 3T3-L1 pre-adipocytes by all-trans retinoic acid: Integrative analysis of transcriptomic and phenotypic data

scientific article

Inhibition of real-time RT-PCR quantification due to tissue-specific contaminants.

scientific article published in February 2004

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

scientific article published on 01 January 2003

Integrative Analysis of MicroRNA and mRNA Data Reveals an Orchestrated Function of MicroRNAs in Skeletal Myocyte Differentiation in Response to TNF-α or IGF1.

scientific article

Inulin and probiotics in newly weaned piglets: effects on intestinal morphology, mRNA expression levels of inflammatory marker genes and haematology

scientific article published on August 2010

Involvement of Pro-Inflammatory Cytokines, Mediators of Inflammation, and Basic Fibroblast Growth Factor in Prostaglandin F2α-Induced Luteolysis in Bovine Corpus Luteum1 ⬇️

scientific article published on October 15, 2003

Letter to The Editor: MicroRNA Profile and Adaptive Response to Exercise Training: A Review

scientific article published on 06 September 2019

Long-term effects of mycophenolic acid on the immunoglobulin and inflammatory marker-gene expression in sheep white blood cells

scientific article published on 07 July 2010

MicroRNA of whole milk samples are not suitable for pregnancy detection in cattle

scientific article published on 11 January 2019

Milking characteristics and their relation to adrenergic receptor mRNA expression and ligand binding in the mammary gland of dairy cows.

scientific article published in October 2003

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

scientific article published on 23 November 2018

Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers

scientific article published on 07 November 2006

Monitoring gene expression in muscle tissue of macaca fascicularis under the influence of testosterone and SARM.

scientific article published on January 2010

New surveillance concepts in food safety in meat producing animals: the advantage of high throughput 'omics' technologies - A review

scientific article published on 31 May 2018

Nicotinic acetylcholine receptor subunits α4 and α5 associated with smoking behaviour and lung cancer are regulated by upstream open reading frames.

scientific article

Non-invasive screening for treatment of heifers with the anabolic steroid melengestrol acetate (MGA) by feces analysis.

scientific article published in January 2003

Normalization strategies for microRNA profiling experiments: a 'normal' way to a hidden layer of complexity?

scientific article published on 12 August 2010

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper

scientific article

Optimization of extraction of circulating RNAs from plasma--enabling small RNA sequencing

scientific article

Pleading for adherence to the MIQE-Guidelines when reporting quantitative PCR data in forensic genetic research

scientific article published on 26 June 2019

Postprandial transfer of colostral extracellular vesicles and their protein and miRNA cargo in neonatal calves

scientific article published on 28 February 2020

Posttranscriptional regulatory networks: from expression profiling to integrative analysis of mRNA and microRNA data.

scientific article

Primer sequence disclosure: a clarification of the MIQE guidelines.

scientific article

Production of CTC-containing porcine reference materials

scientific article published on 01 December 1998

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study

scientific article (publication date: 2012)

Progranulin signaling in sepsis, community-acquired bacterial pneumonia and COVID-19: a comparative, observational study

scientific article published on 03 September 2021

Prostaglandins in Superovulation Induced Bovine Follicles During the Preovulatory Period and Early Corpus Luteum

scientific article published on 10 July 2019

Quality control for quantitative PCR based on amplification compatibility test.

scientific article published on 28 January 2010

Quantification noise in single cell experiments ⬇️

scientific article published on July 11, 2011

Quantification noise in single cell experiments.

scientific article

Quantification of androgen receptor mRNA in tissues by competitive co-amplification of a template in reverse transcription-polymerase chain reaction

scientific article published on 01 August 1996

Quantification of insulin-like growth factor-1 (IGF-1) mRNA: development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction

scientific article published on 01 January 1998

Quantification of insulin-like growth factor-1 (IGF-1) mRNA: modulation of growth intensity by feeding results in inter- and intra-tissue-specific differences of IGF-1 mRNA expression in steers.

scientific article published in January 1998

Quantitative mRNA analysis of eight bovine 5-HT receptor subtypes in brain, abomasum, and intestine by real-time RT-PCR.

scientific article published in January 2003

Quantitative real-time RT-PCR--a perspective.

scientific article

RDML-Ninja and RDMLdb for standardized exchange of qPCR data

scientific article

RNA integrity and the effect on the real-time qRT-PCR performance

scientific article

RNA-Sequencing as Useful Screening Tool in the Combat against the Misuse of Anabolic Agents

scientific article published in 2012

Real-time RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding proteins 1, 2 and 3 in the bovine species

scientific article published on 01 April 2002

RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization

scientific article

Reference histology of veal calf genital and endocrine tissues - an update for screening on hormonal growth promoters.

scientific article published in June 2007

Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR

scientific article (publication date: May 2002)

Reply

scientific article published on 12 July 2016

Shisha microbiota: the good, the bad and the not so ugly.

scientific article published on 6 July 2018

Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis.

scientific article published in March 2004

Short-term effects on pro-inflammatory cytokine, lactoferrin and CD14 mRNA expression levels in bovine immunoseparated milk and blood cells treated by LPS.

scientific article

Standardized determination of real-time PCR efficiency from a single reaction set-up

scientific article

Stimulated enrichment of Clostridium difficile specific IgA in mature cow's milk.

scientific article published on 25 April 2018

Synergetic downregulation of 67 kDa laminin receptor by the green tea (Camellia sinensis) secondary plant compound epigallocatechin gallate: a new gateway in metastasis prevention?

scientific article

TNF-α and IGF1 modify the microRNA signature in skeletal muscle cell differentiation

scientific article

Tailoring the resolution of single-cell RNA sequencing for primary cytotoxic T cells

scientific article published on 25 January 2021

The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression

scientific article published on 15 December 2017

The Emerging Role of miRNAs for the Radiation Treatment of Pancreatic Cancer

scientific article published on 09 December 2020

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

scientific article (publication date: April 2009)

The analysis of the transcriptome as a new approach for biomarker development to trace the abuse of anabolic steroid hormones

scientific article published on 06 September 2011

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

scientific article

The effects of branched-chain amino acid interactions on growth performance, blood metabolites, enzyme kinetics and transcriptomics in weaned pigs.

scientific article published on 3 March 2010

The gastrointestinal tract as target of steroid hormone action: quantification of steroid receptor mRNA expression (AR, ERalpha, ERbeta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR.

scientific article published in February 2003

The immunomodulatory effect of lactulose on Enterococcus faecium fed preruminant calves.

scientific article

The influence of polyphenol rich apple pomace or red-wine pomace diet on the gut morphology in weaning piglets.

scientific article published in August 2007

The mRNA expression of insulin receptor isoforms (IR-A and IR-B) and IGFR-2 in the bovine corpus luteum during the estrous cycle, pregnancy, and induced luteolysis

scientific article published on 01 November 2003

The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced luteolysis.

scientific article

The need for transparency and good practices in the qPCR literature.

scientific article published in November 2013

The ongoing evolution of qPCR.

scientific article published in April 2010

The physiological way: monitoring RNA expression changes as new approach to combat illegal growth promoter application

scientific article published on 01 August 2012

The potential of bovine vaginal smear for biomarker development to trace the misuse of anabolic agents.

scientific article published on 28 October 2010

The potential of circulating extracellular small RNAs (smexRNA) in veterinary diagnostics-Identifying biomarker signatures by multivariate data analysis

scientific article

The use of omic technologies for biomarker development to trace functions of anabolic agents

scientific article published on 05 February 2009

Tissue-specific expression pattern of bovine prion gene: quantification using real-time RT-PCR.

scientific article published in February 2003

Tissue-specific expression pattern of estrogen receptors (ER): quantification of ER alpha and ER beta mRNA with real-time RT-PCR.

scientific article published in May 2001

Toward reliable biomarker signatures in the age of liquid biopsies - how to standardize the small RNA-Seq workflow

scientific article

Transcriptional biomarkers--high throughput screening, quantitative verification, and bioinformatical validation methods

scientific article published on 04 September 2012

Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera

scientific article published on 27 September 2019

Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model

scientific article published on 06 February 2019

Tumor Necrosis Factor Alpha and Insulin-Like Growth Factor 1 Induced Modifications of the Gene Expression Kinetics of Differentiating Skeletal Muscle Cells

scientific article published on 8 October 2015

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

scientific article

Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses

scientific article published on 30 September 2009

[Insulin-like growth factor 1 (IGF-1) mRNA and IGF-1 protein. Expression in cells of the trabecular meshwork of the bovine eye]

scientific article

mRNA and microRNA purity and integrity: the key to success in expression profiling.

scientific article

miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity

scientific article

microRNA in native and processed cow's milk and its implication for the farm milk effect on asthma

scientific article published on 18 December 2015

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