List of works - Nobutaka Nakashima

A new carbon catabolite repression mutation of Escherichia coli, mlc∗, and its use for producing isobutanol

scientific article

A novel system for expressing recombinant proteins over a wide temperature range from 4 to 35 degrees C.

scientific article published in April 2004

A vector library for silencing central carbon metabolism genes with antisense RNAs in Escherichia coli.

scientific article

Actinomycetes as host cells for production of recombinant proteins

scientific article

Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae.

scientific article

Bacterial cellular engineering by genome editing and gene silencing

scientific article published on 18 February 2014

Bacterial production of isobutanol without expensive reagents.

scientific article published on 31 October 2014

Cell-free protein synthesis using cell extract of Pseudomonas fluorescens and CspA promoter.

scientific article published in June 2004

Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase.

scientific article

Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli

scientific article

Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli.

scientific article published on 10 June 2009

Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli.

scientific article published on 23 April 2015

Draft Genome Sequence of Burkholderia sp. Strain CCA53, Isolated from Leaf Soil

scientific article published on 07 July 2016

Establishment of a multi-species biofilm model and metatranscriptomic analysis of biofilm and planktonic cell communities.

scientific article

Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin.

scientific article published on 7 August 2014

Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline-inducible vectors.

scientific article published on 27 March 2013

Genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from Rhodococcus opacus strain SAO101.

scientific article

Identification and characterization of Burkholderia multivorans CCA53.

scientific article

Identification of a methanol-inducible promoter from Rhodococcus erythropolis PR4 and its use as an expression vector

scientific article published on 26 January 2012

Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

scientific article

Isolation and characterization of a rolling-circle-type plasmid from Rhodococcus erythropolis and application of the plasmid to multiple-recombinant-protein expression

scientific article (publication date: September 2004)

MazF activation causes ACA sequence-independent and selective alterations in RNA levels in Escherichia coli

scientific article published on 04 September 2019

Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1.

scientific article published on 27 July 2015

Multiple-gene silencing using antisense RNAs in Escherichia coli.

scientific article published in January 2012

Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B

scientific article

Production of acetoin from hydrothermally pretreated oil mesocarp fiber using metabolically engineered Escherichia coli in a bioreactor system

scientific article published on 31 August 2017

Production of d-lactate using a pyruvate-producing Escherichia coli strain

scientific article

Pyruvate production using engineered Escherichia coli

scientific article (publication date: December 2016)

Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p.

scientific article

List of works by Nobutaka Nakashima

A new carbon catabolite repression mutation of Escherichia coli, mlc∗, and its use for producing isobutanol

A novel system for expressing recombinant proteins over a wide temperature range from 4 to 35 degrees C.

A vector library for silencing central carbon metabolism genes with antisense RNAs in Escherichia coli.

Actinomycetes as host cells for production of recombinant proteins

Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae.

Bacterial cellular engineering by genome editing and gene silencing

Bacterial production of isobutanol without expensive reagents.

Cell-free protein synthesis using cell extract of Pseudomonas fluorescens and CspA promoter.

Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase.

Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli

Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli.

Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli.

Draft Genome Sequence of Burkholderia sp. Strain CCA53, Isolated from Leaf Soil

Establishment of a multi-species biofilm model and metatranscriptomic analysis of biofilm and planktonic cell communities.

Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin.

Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline-inducible vectors.

Genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from Rhodococcus opacus strain SAO101.

Identification and characterization of Burkholderia multivorans CCA53.

Identification of a methanol-inducible promoter from Rhodococcus erythropolis PR4 and its use as an expression vector

Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

Isolation and characterization of a rolling-circle-type plasmid from Rhodococcus erythropolis and application of the plasmid to multiple-recombinant-protein expression

MazF activation causes ACA sequence-independent and selective alterations in RNA levels in Escherichia coli

Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1.

Multiple-gene silencing using antisense RNAs in Escherichia coli.

Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B

Production of acetoin from hydrothermally pretreated oil mesocarp fiber using metabolically engineered Escherichia coli in a bioreactor system

Production of d-lactate using a pyruvate-producing Escherichia coli strain

Pyruvate production using engineered Escherichia coli

Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p.