List of works - Ray Owens

A RANKL mutant used as an inter-species vaccine for efficient immunotherapy of osteoporosis.

scientific article

A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells

scientific article

A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization

scientific article (publication date: June 2005)

A streamlined implementation of the glutamine synthetase-based protein expression system.

scientific article

Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications ⬇️

scientific article

Affimer proteins are versatile and renewable affinity reagents ⬇️

scientific article

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

scientific article

Application of high-throughput technologies to a structural proteomics-type analysis of Bacillus anthracis.

scientific article published on 19 September 2006

Benefits of automated crystallization plate tracking, imaging, and analysis

scientific article

Chromophore-linked substrate (CLS405): probing metallo-β-lactamase activity and inhibition.

scientific article

Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase.

scientific article

Conformational changes in IgE contribute to its uniquely slow dissociation rate from receptor FcɛRI.

scientific article

Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells.

scientific article published in January 2012

Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication

scientific article

Crystal Structures of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport

scientific article

Crystal structure of a 3-oxoacyl-(acylcarrier protein) reductase (BA3989) from Bacillus anthracis at 2.4-A resolution

scientific article

Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis.

scientific article

Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues

scientific article

Crystal structures of penicillin-binding protein 3 in complexes with azlocillin and cefoperazone in both acylated and deacylated forms

scientific article published on 23 January 2016

Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL ⬇️

scientific article published on 22 May 2009

Development of a recombinant cell-based system for the characterisation of phosphodiesterase 4 isoforms and evaluation of inhibitors

article

Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity

scientific article published on 19 November 2012

Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19

scientific article published on 27 July 2020

Exon structure of the collagen-binding domain of human fibronectin

scientific article published on 01 August 1986

Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters.

scientific article published in January 2016

Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.

scientific article published on 25 April 2013

Expression, purification and crystallization of a lyssavirus matrix (M) protein

scientific article

GFP-based expression screening of membrane proteins in insect cells using the baculovirus system

scientific article published on January 2015

Genetic modification of a baculovirus vector for increased expression in insect cells.

scientific article

Green fluorescent protein-based expression screening of membrane proteins in Escherichia coli.

scientific article published on 6 January 2015

High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multidomain flavoenzyme-signaling molecule

scientific article

High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate

scientific article published on 23 September 2015

Highly stable single-strand-specific 3'-nuclease/nucleotidase from Legionella pneumophila.

scientific article published on 23 March 2018

Identification of Contact Residues in the IgE Binding Site of Human FcεRIα†

scientific article published on 01 December 1997

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

scientific article published on 19 September 2006

Improved expression of secreted and membrane-targeted proteins in insect cells.

scientific article published on 21 June 2010

In situ macromolecular crystallography using microbeams

scientific article published on 17 April 2012

In vitro and in vivo characterization of the multiple isoforms of Schistosoma mansoni hypoxanthine-guanine phosphoribosyltransferases

scientific article published on 14 February 2019

Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli.

scientific article

Methods in integrated structural biology.

scientific article published on 24 December 2015

Methods in structural proteomics.

scientific article

Molecular cloning and expression of a human phosphodiesterase 4C.

scientific article published in December 1997

Novel nucleotide triphosphates as potent P2Y2 agonists

scientific article published on 29 September 2006

Novel nucleotide triphosphates as potent P2Y2 agonists with enhanced stability over UTP.

scientific article published on 19 October 2006

PDE 4 inhibitors: the use of molecular cloning in the design and development of novel drugs

article

Preparation and preclinical evaluation of humanised A33 immunoconjugates for radioimmunotherapy.

scientific article published on December 1995

S-Adenosyl-S-carboxymethyl-L-homocysteine: a novel cofactor found in the putative tRNA-modifying enzyme CmoA

scientific article

Sample preparation and mass-spectrometric characterization of crystal-derived protein samples

scientific article published on 20 April 2005

Secretion of recombinant human IgE-Fc by mammalian cells and biological activity of glycosylation site mutants

article

Some lessons from the systematic production and structural analysis of soluble (alpha)(beta) T-cell receptors

scientific article published on 26 August 2009

Streamlining the production of proteins for structural biology

scientific article published on 27 June 2019

Structural Plasticity of Eph-Receptor A4 Facilitates Cross-Class Ephrin Signaling.

scientific article published on 8 December 2009

Structural Proteomics

scientific article published on 01 January 2015

Structural and Functional Characterization of the Kindlin-1 Pleckstrin Homology Domain

scientific article

Structural and kinetic analysis of Schistosoma mansoni Adenylosuccinate Lyase (SmADSL).

scientific article published on 24 March 2017

Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient

scientific article published on 31 July 2020

Structure and Assembly of a Trans-Periplasmic Channel for Type IV Pili in Neisseria meningitidis

scientific article

Structure of 5-formyltetrahydrofolate cyclo-ligase fromBacillus anthracis(BA4489)

scientific article

Structure of ribose 5-phosphate isomerase from the probiotic bacteriumLactobacillus salivariusUCC118

scientific article

Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 A resolution.

scientific article published on 31 May 2006

Structure of the cold-shock domain protein fromNeisseria meningitidisreveals a strand-exchanged dimer

scientific article

Structure of the regulatory domain of the LysR family regulator NMB2055 (MetR-like protein) fromNeisseria meningitidis

scientific article

Substituted 2-(2-aminopyrimidin-4-yl)pyridine-4-carboxylates as potent inhibitors of JumonjiC domain-containing histone demethylases

scientific article

Synthesis and characterisation of 5-acyl-6,7-dihydrothieno[3,2-c]pyridine inhibitors of Hedgehog acyltransferase

scientific article published on 10 February 2016

The crystal structure of IgE Fc reveals an asymmetrically bent conformation

scientific article

The genetic engineering of monoclonal antibodies.

scientific article published on February 1994

The impact of protein characterization in structural proteomics.

scientific article

The in vivo and in vitro characterisation of an engineered human antibody to E-selectin

scientific article published on 01 June 1997

The nsp9 replicase protein of SARS-coronavirus, structure and functional insights

scientific article

The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning.

scientific article

The production of glycoproteins by transient expression in Mammalian cells.

scientific article

The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators

scientific article

The structure of NMB1585, a MarR-family regulator fromNeisseria meningitidis

scientific article

The structure of nontypeable Haemophilus influenzae SapA in a closed conformation reveals a constricted ligand-binding cavity and a novel RNA binding motif

scientific article published on 15 October 2021

The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension

scientific article

Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins.

scientific article

Vaccinia Virus Proteins A52 and B14 Share a Bcl-2–Like Fold but Have Evolved to Inhibit NF-κB rather than Apoptosis

scientific article

xtalPiMS: a PiMS-based web application for the management and monitoring of crystallization trials

scientific article

List of works by Ray Owens

A RANKL mutant used as an inter-species vaccine for efficient immunotherapy of osteoporosis.

A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells

A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization

A streamlined implementation of the glutamine synthetase-based protein expression system.

Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications ⬇️

Affimer proteins are versatile and renewable affinity reagents ⬇️

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

Application of high-throughput technologies to a structural proteomics-type analysis of Bacillus anthracis.

Benefits of automated crystallization plate tracking, imaging, and analysis

Chromophore-linked substrate (CLS405): probing metallo-β-lactamase activity and inhibition.

Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase.

Conformational changes in IgE contribute to its uniquely slow dissociation rate from receptor FcɛRI.

Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells.

Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication

Crystal Structures of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport

Crystal structure of a 3-oxoacyl-(acylcarrier protein) reductase (BA3989) from Bacillus anthracis at 2.4-A resolution

Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis.

Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues

Crystal structures of penicillin-binding protein 3 in complexes with azlocillin and cefoperazone in both acylated and deacylated forms

Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL ⬇️

Development of a recombinant cell-based system for the characterisation of phosphodiesterase 4 isoforms and evaluation of inhibitors

Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity

Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19

Exon structure of the collagen-binding domain of human fibronectin

Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters.

Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.

Expression, purification and crystallization of a lyssavirus matrix (M) protein

GFP-based expression screening of membrane proteins in insect cells using the baculovirus system

Genetic modification of a baculovirus vector for increased expression in insect cells.

Green fluorescent protein-based expression screening of membrane proteins in Escherichia coli.

High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multidomain flavoenzyme-signaling molecule

High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate

Highly stable single-strand-specific 3'-nuclease/nucleotidase from Legionella pneumophila.

Identification of Contact Residues in the IgE Binding Site of Human FcεRIα†

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

Improved expression of secreted and membrane-targeted proteins in insect cells.

In situ macromolecular crystallography using microbeams

In vitro and in vivo characterization of the multiple isoforms of Schistosoma mansoni hypoxanthine-guanine phosphoribosyltransferases

Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli.

Methods in integrated structural biology.

Methods in structural proteomics.

Molecular cloning and expression of a human phosphodiesterase 4C.

Novel nucleotide triphosphates as potent P2Y2 agonists

Novel nucleotide triphosphates as potent P2Y2 agonists with enhanced stability over UTP.

PDE 4 inhibitors: the use of molecular cloning in the design and development of novel drugs

Preparation and preclinical evaluation of humanised A33 immunoconjugates for radioimmunotherapy.

S-Adenosyl-S-carboxymethyl-L-homocysteine: a novel cofactor found in the putative tRNA-modifying enzyme CmoA

Sample preparation and mass-spectrometric characterization of crystal-derived protein samples

Secretion of recombinant human IgE-Fc by mammalian cells and biological activity of glycosylation site mutants

Some lessons from the systematic production and structural analysis of soluble (alpha)(beta) T-cell receptors

Streamlining the production of proteins for structural biology

Structural Plasticity of Eph-Receptor A4 Facilitates Cross-Class Ephrin Signaling.

Structural Proteomics

Structural and Functional Characterization of the Kindlin-1 Pleckstrin Homology Domain

Structural and kinetic analysis of Schistosoma mansoni Adenylosuccinate Lyase (SmADSL).

Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient

Structure and Assembly of a Trans-Periplasmic Channel for Type IV Pili in Neisseria meningitidis

Structure of 5-formyltetrahydrofolate cyclo-ligase fromBacillus anthracis(BA4489)

Structure of ribose 5-phosphate isomerase from the probiotic bacteriumLactobacillus salivariusUCC118

Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 A resolution.

Structure of the cold-shock domain protein fromNeisseria meningitidisreveals a strand-exchanged dimer

Structure of the regulatory domain of the LysR family regulator NMB2055 (MetR-like protein) fromNeisseria meningitidis

Substituted 2-(2-aminopyrimidin-4-yl)pyridine-4-carboxylates as potent inhibitors of JumonjiC domain-containing histone demethylases

Synthesis and characterisation of 5-acyl-6,7-dihydrothieno[3,2-c]pyridine inhibitors of Hedgehog acyltransferase

The crystal structure of IgE Fc reveals an asymmetrically bent conformation

The genetic engineering of monoclonal antibodies.

The impact of protein characterization in structural proteomics.

The in vivo and in vitro characterisation of an engineered human antibody to E-selectin

The nsp9 replicase protein of SARS-coronavirus, structure and functional insights

The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning.

The production of glycoproteins by transient expression in Mammalian cells.

The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators

The structure of NMB1585, a MarR-family regulator fromNeisseria meningitidis

The structure of nontypeable Haemophilus influenzae SapA in a closed conformation reveals a constricted ligand-binding cavity and a novel RNA binding motif

The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension

Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins.

Vaccinia Virus Proteins A52 and B14 Share a Bcl-2–Like Fold but Have Evolved to Inhibit NF-κB rather than Apoptosis

xtalPiMS: a PiMS-based web application for the management and monitoring of crystallization trials