List of works - Akeo Shinkai

A non-cold-inducible cold shock protein homolog mainly contributes to translational control under optimal growth conditions.

scientific article

An alkyltransferase-like protein from Thermus thermophilus HB8 affects the regulation of gene expression in alkylation response

article

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus

scientific article published on 17 January 2007

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of molybdopterin synthase from Thermus thermophilus HB8.

scientific article published on 30 March 2007

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of the putative SAICAR synthetase (PH0239) from Pyrococcus horikoshii OT3.

scientific article

Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA

scientific article

Crystal structure of ST2348, a CBS domain protein, from hyperthermophilic archaeon Sulfolobus tokodaii

scientific article

Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine and AMPPNP

scientific article

Crystal structure of TTHA0807, a CcpA regulator, from Thermus thermophilus HB8

scientific article

Crystal structure of an archaeal specific DNA-binding protein (Ape10b2) from Aeropyrum pernix K1.

scientific article published in May 2008

Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding

scientific article

Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli

scientific article

Crystal structure of hypothetical protein TTHB210, controlled by the σ(E) /anti-σ(E) regulatory system in Thermus thermophilus HB8, reveals a novel homodecamer

scientific article

Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7

scientific article

Crystal structure of the manganese transport regulatory protein from Escherichia coli

scientific article

Crystal structure of uncharacterized protein TTHA1756 from Thermus thermophilus HB8: Structural variety in UPF0150 family proteins

scientific article

Crystal structures, dynamics and functional implications of molybdenum-cofactor biosynthesis protein MogA from two thermophilic organisms

scientific article

Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus

scientific article published on 16 December 2006

Crystallization and preliminary crystallographic studies of L30e, a ribosomal protein from Methanocaldococcus jannaschii (MJ1044)

scientific article published on 31 January 2008

Crystallization screening test for the whole-cell project on Thermus thermophilus HB8.

scientific article published on 30 May 2008

Expression, purification and X-ray analysis of 1,3-propanediol dehydrogenase (Aq_1145) from Aquifex aeolicus VF5.

scientific article

Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii

scientific article

Free and ATP-bound structures of Ap4A hydrolase from Aquifex aeolicus V5

scientific article

Functional identification of an anti-sigmaE factor from Thermus thermophilus HB8.

scientific article published on 18 July 2008

Genome-wide comprehensive analysis of transcriptional regulation by ArgR in Thermus thermophilus

scientific article published on 29 July 2014

Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator

scientific article

High-level expression and purification of a recombinant human alpha-1, 3-fucosyltransferase in baculovirus-infected insect cells.

scientific article published on August 1997

Hyper-thermostability of CutA1 protein, with a denaturation temperature of nearly 150 degrees C.

scientific article

Identification of novel genes regulated by the oxidative stress-responsive transcriptional activator SdrP in Thermus thermophilus HB8

scientific journal article

Identification of promoters recognized by RNA polymerase-sigmaE holoenzyme from Thermus thermophilus HB8.

scientific article

In vivo mutagenesis by Escherichia coli DNA polymerase I. Ile(709) in motif A functions in base selection.

scientific article

Integrated database of information from structural genomics experiments

scientific article published on 19 April 2013

LdrP, a cAMP receptor protein/FNR family transcriptional regulator, serves as a positive regulator for the light-inducible gene cluster in the megaplasmid of Thermus thermophilus

scientific article published on 7 October 2014

Mapping of the Sites Involved in Ligand Association and Dissociation at the Extracellular Domain of the Kinase Insert Domain-containing Receptor for Vascular Endothelial Growth Factor

scientific article published in Journal of Biological Chemistry

New structural insights and molecular-modelling studies of 4-methyl-5-beta-hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK)

scientific article published on 18 September 2009

Phenylacetyl coenzyme A is an effector molecule of the TetR family transcriptional repressor PaaR from Thermus thermophilus HB8.

scientific article

Preliminary X-ray crystallographic study of glucose dehydrogenase from Thermus thermophilus HB8

scientific article published on 28 April 2007

Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators isolated from Sulfolobus tokodaii strain 7

scientific article published on 24 October 2007

Purification, crystallization and preliminary X-ray diffraction analysis of the putative ABC transporter ATP-binding protein from Thermotoga maritima

scientific article published on 17 May 2008

Quantitative renaturation from a guanidine-denatured state of the SecA dimer, a 200 KDa protein involved in protein secretion in Escherichia coli

scientific article published on November 1990

RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus

scientific article

SecA, an essential component of the secretory machinery of Escherichia coli, exists as homodimer.

scientific article published in January 1991

Structural and functional characterization of the transcriptional repressor CsoR from Thermus thermophilus HB8

scientific article

Structural biology. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning

scientific article

Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus

scientific article published in October 2013

Structure and function of a TetR family transcriptional regulator, SbtR, from thermus thermophilus HB8

scientific article

Structure of D-alanine-D-alanine ligase from Thermus thermophilus HB8: cumulative conformational change and enzyme-ligand interactions.

scientific article published on 16 September 2009

Structure of SurE protein fromAquifex aeolicusVF5 at 1.5 Å resolution

scientific article

Structure of a UPF0150-family protein fromThermus thermophilusHB8

scientific article

Structure of a putative β-phosphoglucomutase (TM1254) fromThermotoga maritima

scientific article

Structure of glyceraldehyde-3-phosphate dehydrogenase from the archaeal hyperthermophileMethanocaldococcus jannaschii

scientific article

Structure of hypothetical Mo-cofactor biosynthesis protein B (ST2315) fromSulfolobus tokodaii

scientific article

Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8.

scientific article

Structures of apo and GTP-bound molybdenum cofactor biosynthesis protein MoaC from Thermus thermophilus HB8

scientific article

Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) fromThermus thermophilusHB8

scientific article

Substrate specificity determinants of the methanogen homoaconitase enzyme: structure and function of the small subunit

scientific article

TetR-family transcriptional repressor Thermus thermophilus FadR controls fatty acid degradation

scientific article

The putative DNA-binding protein Sto12a from the thermoacidophilic archaeon Sulfolobus tokodaii contains intrachain and interchain disulfide bonds

scientific article

The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8.

scientific article

Transcription activation mediated by a cyclic AMP receptor protein from Thermus thermophilus HB8

scientific journal article

Transcription profile of Thermus thermophilus CRISPR systems after phage infection.

scientific article published on 3 November 2009

Transcriptional Repression Mediated by a TetR Family Protein, PfmR, from Thermus thermophilus HB8

scientific article

Very rapid induction of a cold shock protein by temperature downshift in Thermus thermophilus.

scientific article

X-ray crystal structure of TTHB099, a CRP/FNR superfamily transcriptional regulator from Thermus thermophilus HB8, reveals a DNA-binding protein with no required allosteric effector molecule

scientific article

X-ray crystal structure of a CRISPR-associated RAMP module [corrected] Cmr5 protein [corrected] from Thermus thermophilus HB8

scientific article

X-ray crystal structure of a CRISPR-associated protein, Cse2, from Thermus thermophilus HB8

scientific article

X-ray crystal structure of a hypothetical Sua5 protein from Sulfolobus tokodaii strain 7

scientific article

List of works by Akeo Shinkai

A non-cold-inducible cold shock protein homolog mainly contributes to translational control under optimal growth conditions.

An alkyltransferase-like protein from Thermus thermophilus HB8 affects the regulation of gene expression in alkylation response

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of molybdopterin synthase from Thermus thermophilus HB8.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of the putative SAICAR synthetase (PH0239) from Pyrococcus horikoshii OT3.

Crystal structure and RNA-binding analysis of the archaeal transcription factor NusA

Crystal structure of ST2348, a CBS domain protein, from hyperthermophilic archaeon Sulfolobus tokodaii

Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine and AMPPNP

Crystal structure of TTHA0807, a CcpA regulator, from Thermus thermophilus HB8

Crystal structure of an archaeal specific DNA-binding protein (Ape10b2) from Aeropyrum pernix K1.

Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding

Crystal structure of hydrogenase maturating endopeptidase HycI from Escherichia coli

Crystal structure of hypothetical protein TTHB210, controlled by the σ(E) /anti-σ(E) regulatory system in Thermus thermophilus HB8, reveals a novel homodecamer

Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7

Crystal structure of the manganese transport regulatory protein from Escherichia coli

Crystal structure of uncharacterized protein TTHA1756 from Thermus thermophilus HB8: Structural variety in UPF0150 family proteins

Crystal structures, dynamics and functional implications of molybdenum-cofactor biosynthesis protein MogA from two thermophilic organisms

Crystallization and preliminary crystallographic analysis of molybdenum-cofactor biosynthesis protein C from Thermus thermophilus

Crystallization and preliminary crystallographic studies of L30e, a ribosomal protein from Methanocaldococcus jannaschii (MJ1044)

Crystallization screening test for the whole-cell project on Thermus thermophilus HB8.

Expression, purification and X-ray analysis of 1,3-propanediol dehydrogenase (Aq_1145) from Aquifex aeolicus VF5.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii

Free and ATP-bound structures of Ap4A hydrolase from Aquifex aeolicus V5

Functional identification of an anti-sigmaE factor from Thermus thermophilus HB8.

Genome-wide comprehensive analysis of transcriptional regulation by ArgR in Thermus thermophilus

Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator

High-level expression and purification of a recombinant human alpha-1, 3-fucosyltransferase in baculovirus-infected insect cells.

Hyper-thermostability of CutA1 protein, with a denaturation temperature of nearly 150 degrees C.

Identification of novel genes regulated by the oxidative stress-responsive transcriptional activator SdrP in Thermus thermophilus HB8

Identification of promoters recognized by RNA polymerase-sigmaE holoenzyme from Thermus thermophilus HB8.

In vivo mutagenesis by Escherichia coli DNA polymerase I. Ile(709) in motif A functions in base selection.

Integrated database of information from structural genomics experiments

LdrP, a cAMP receptor protein/FNR family transcriptional regulator, serves as a positive regulator for the light-inducible gene cluster in the megaplasmid of Thermus thermophilus

Mapping of the Sites Involved in Ligand Association and Dissociation at the Extracellular Domain of the Kinase Insert Domain-containing Receptor for Vascular Endothelial Growth Factor

New structural insights and molecular-modelling studies of 4-methyl-5-beta-hydroxyethylthiazole kinase from Pyrococcus horikoshii OT3 (PhThiK)

Phenylacetyl coenzyme A is an effector molecule of the TetR family transcriptional repressor PaaR from Thermus thermophilus HB8.

Preliminary X-ray crystallographic study of glucose dehydrogenase from Thermus thermophilus HB8

Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators isolated from Sulfolobus tokodaii strain 7

Purification, crystallization and preliminary X-ray diffraction analysis of the putative ABC transporter ATP-binding protein from Thermotoga maritima

Quantitative renaturation from a guanidine-denatured state of the SecA dimer, a 200 KDa protein involved in protein secretion in Escherichia coli

RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus

SecA, an essential component of the secretory machinery of Escherichia coli, exists as homodimer.

Structural and functional characterization of the transcriptional repressor CsoR from Thermus thermophilus HB8

Structural biology. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning

Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus

Structure and function of a TetR family transcriptional regulator, SbtR, from thermus thermophilus HB8

Structure of D-alanine-D-alanine ligase from Thermus thermophilus HB8: cumulative conformational change and enzyme-ligand interactions.

Structure of SurE protein fromAquifex aeolicusVF5 at 1.5 Å resolution

Structure of a UPF0150-family protein fromThermus thermophilusHB8

Structure of a putative β-phosphoglucomutase (TM1254) fromThermotoga maritima

Structure of glyceraldehyde-3-phosphate dehydrogenase from the archaeal hyperthermophileMethanocaldococcus jannaschii

Structure of hypothetical Mo-cofactor biosynthesis protein B (ST2315) fromSulfolobus tokodaii

Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8.

Structures of apo and GTP-bound molybdenum cofactor biosynthesis protein MoaC from Thermus thermophilus HB8

Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) fromThermus thermophilusHB8

Substrate specificity determinants of the methanogen homoaconitase enzyme: structure and function of the small subunit

TetR-family transcriptional repressor Thermus thermophilus FadR controls fatty acid degradation

The putative DNA-binding protein Sto12a from the thermoacidophilic archaeon Sulfolobus tokodaii contains intrachain and interchain disulfide bonds

The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8.

Transcription activation mediated by a cyclic AMP receptor protein from Thermus thermophilus HB8

Transcription profile of Thermus thermophilus CRISPR systems after phage infection.

Transcriptional Repression Mediated by a TetR Family Protein, PfmR, from Thermus thermophilus HB8

Very rapid induction of a cold shock protein by temperature downshift in Thermus thermophilus.

X-ray crystal structure of TTHB099, a CRP/FNR superfamily transcriptional regulator from Thermus thermophilus HB8, reveals a DNA-binding protein with no required allosteric effector molecule

X-ray crystal structure of a CRISPR-associated RAMP module [corrected] Cmr5 protein [corrected] from Thermus thermophilus HB8

X-ray crystal structure of a CRISPR-associated protein, Cse2, from Thermus thermophilus HB8

X-ray crystal structure of a hypothetical Sua5 protein from Sulfolobus tokodaii strain 7