List of works - Hideo Iwai

A monomeric TIM-barrel structure fromPyrococcus furiosusis optimized for extreme temperatures ⬇️

scientific article published on October 13, 2012

Assignments of 1H and 15N resonances of the bacteriophage lambda capsid stabilizing protein gpD.

scientific article

Biosynthesis of the Bis-Prenylated Alkaloids Muscoride A and B

scientific article published on 19 November 2019

Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR.

scientific article published on 20 November 2003

Circular β-lactamase: stability enhancement by cyclizing the backbone

scientific article published on 01 October 1999

Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

scientific article

Conformational changes of the BS2 operator DNA upon complex formation with the Antennapedia homeodomain studied by NMR with 13C/15N-labeled DNA.

scientific article

Conformational dynamics as a key factor of signaling mediated by the receiver domain of sensor histidine kinase from Arabidopsis thaliana

scientific article published on 31 August 2017

Crystal structures of CDC21-1 inteins from hyperthermophilic archaea reveal the selection mechanism for the highly conserved homing endonuclease insertion site

scientific article published on 30 July 2019

Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus.

scientific article

Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR.

scientific article published on 28 November 2017

Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli

scientific article

Heterogeneous dynamics in partially disordered proteins

scientific article published on 15 September 2020

Hidden photoinduced reactivity of the blue fluorescent protein mKalama1

scientific article

Hideo Iwai. Interview by Daniela Ruffell.

scientific article

Highly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiforme

scientific article published on 24 February 2006

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies

scientific article

Intermolecular domain swapping induces intein-mediated protein alternative splicing

scientific article published in October 2013

Is coproporphyrin III a copper-acquisition compound in Paracoccus denitrificans? ⬇️

scientific article published on January 7, 2011

Mutations in the U11/U12-65K protein associated with isolated growth hormone deficiency lead to structural destabilization and impaired binding of U12 snRNA.

scientific article published on 18 December 2017

NMR and Crystal Structures of the Pyrococcus horikoshii RadA Intein Guide a Strategy for Engineering a Highly Efficient and Promiscuous Intein

scientific article

NMR resonance assignment of DnaE intein from Nostoc punctiforme.

scientific article published on 13 January 2009

NMR solution structure of the monomeric form of the bacteriophage λ capsid stabilizing protein gpD

scientific article published on 01 April 2005

NMR structure of the C-terminal domain of TonB protein from

scientific article published on 27 August 2018

NMR structures of salt-refolded forms of the 434-repressor DNA-binding domain in 6 M urea

scientific article

NMR with (13)C, (15)N-doubly-labeled DNA: The shape Antennapedia homeodomain complex with a 14-mer DNA duplex

scientific article published in July 1998

Nature's recipe for splitting inteins

scientific article

Off-Pathway-Sensitive Protein-Splicing Screening Based on a Toxin/Antitoxin System

scientific article published on 03 July 2019

Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR.

scientific article published on 15 January 2018

Protein ligation: applications in NMR studies of proteins.

scientific article published on January 2007

Protein trans-splicing and its use in structural biology: opportunities and limitations.

scientific article published on 31 August 2010

Rotational Dynamics of Proteins from Spin Relaxation Times and Molecular Dynamics Simulations

article

Salt-inducible protein splicing in cis and trans by inteins from extremely halophilic archaea as a novel protein-engineering tool

scientific article

Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-Splicing

article

Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation

scientific article published on 13 March 2018

Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing.

scientific article

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein

scientific article

Segmental isotopic labeling of a single-domain globular protein without any refolding step by an asparaginyl endopeptidase

scientific article published on 30 March 2017

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro

scientific article

Solution conformation of an antibacterial peptide, sarcotoxin IA, as determined by 1H-NMR.

scientific article

Structural Basis for Complement Evasion by Lyme Disease Pathogen Borrelia burgdorferi

scientific article

Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins.

scientific article

Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins

scientific article published on 16 March 2022

Structure of cyclized green fluorescent protein

scientific article

Structure-based engineering and comparison of novel split inteins for protein ligation

scientific article

Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis

scientific article published on 17 August 2020

Tandem SUMO fusion vectors for improving soluble protein expression and purification

scientific article

The 2D NMR experiments H(C)CO2 and HCCO2 for assignment and pH titration of carboxylate groups in uniformly 15N/13C-labeled proteins

scientific article published on 01 January 1997

The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms

scientific article published on 07 November 2020

The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins

scientific article published on 30 October 2019

Use of biosynthetic fractional 13C-labeling for backbone NMR assignment of proteins

scientific article published on 13 January 2007

List of works by Hideo Iwai

A monomeric TIM-barrel structure fromPyrococcus furiosusis optimized for extreme temperatures ⬇️

Assignments of 1H and 15N resonances of the bacteriophage lambda capsid stabilizing protein gpD.

Biosynthesis of the Bis-Prenylated Alkaloids Muscoride A and B

Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR.

Circular β-lactamase: stability enhancement by cyclizing the backbone

Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

Conformational changes of the BS2 operator DNA upon complex formation with the Antennapedia homeodomain studied by NMR with 13C/15N-labeled DNA.

Conformational dynamics as a key factor of signaling mediated by the receiver domain of sensor histidine kinase from Arabidopsis thaliana

Crystal structures of CDC21-1 inteins from hyperthermophilic archaea reveal the selection mechanism for the highly conserved homing endonuclease insertion site

Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus.

Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR.

Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli

Heterogeneous dynamics in partially disordered proteins

Hidden photoinduced reactivity of the blue fluorescent protein mKalama1

Hideo Iwai. Interview by Daniela Ruffell.

Highly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiforme

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies

Intermolecular domain swapping induces intein-mediated protein alternative splicing

Is coproporphyrin III a copper-acquisition compound in Paracoccus denitrificans? ⬇️

Mutations in the U11/U12-65K protein associated with isolated growth hormone deficiency lead to structural destabilization and impaired binding of U12 snRNA.

NMR and Crystal Structures of the Pyrococcus horikoshii RadA Intein Guide a Strategy for Engineering a Highly Efficient and Promiscuous Intein

NMR resonance assignment of DnaE intein from Nostoc punctiforme.

NMR solution structure of the monomeric form of the bacteriophage λ capsid stabilizing protein gpD

NMR structure of the C-terminal domain of TonB protein from

NMR structures of salt-refolded forms of the 434-repressor DNA-binding domain in 6 M urea

NMR with (13)C, (15)N-doubly-labeled DNA: The shape Antennapedia homeodomain complex with a 14-mer DNA duplex

Nature's recipe for splitting inteins

Off-Pathway-Sensitive Protein-Splicing Screening Based on a Toxin/Antitoxin System

Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR.

Protein ligation: applications in NMR studies of proteins.

Protein trans-splicing and its use in structural biology: opportunities and limitations.

Rotational Dynamics of Proteins from Spin Relaxation Times and Molecular Dynamics Simulations

Salt-inducible protein splicing in cis and trans by inteins from extremely halophilic archaea as a novel protein-engineering tool

Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-Splicing

Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation

Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing.

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein

Segmental isotopic labeling of a single-domain globular protein without any refolding step by an asparaginyl endopeptidase

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro

Solution conformation of an antibacterial peptide, sarcotoxin IA, as determined by 1H-NMR.

Structural Basis for Complement Evasion by Lyme Disease Pathogen Borrelia burgdorferi

Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins.

Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins

Structure of cyclized green fluorescent protein

Structure-based engineering and comparison of novel split inteins for protein ligation

Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis

Tandem SUMO fusion vectors for improving soluble protein expression and purification

The 2D NMR experiments H(C)CO2 and HCCO2 for assignment and pH titration of carboxylate groups in uniformly 15N/13C-labeled proteins

The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms

The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins

Use of biosynthetic fractional 13C-labeling for backbone NMR assignment of proteins