List of works - Robert Kourist

A Multi-Enzymatic Cascade Reaction for the Stereoselective Production of γ-Oxyfunctionalyzed Amino Acids.

scientific article published on 7 April 2016

A One-Pot Cascade Reaction Combining an Encapsulated Decarboxylase with a Metathesis Catalyst for the Synthesis of Bio-Based Antioxidants

scientific article

A Reconstructed Common Ancestor of the Fatty Acid Photo-decarboxylase Clade Shows Photo-decarboxylation Activity and Increased Thermostability

scientific article published on 31 March 2021

A Structural View on the Stereospecificity of Plant Borneol-Type Dehydrogenases

scientific article published on 10 March 2021

A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts

scientific article published on 13 October 2015

A combined experimental and modelling approach for the Weimberg pathway optimisation

scientific article published on 27 February 2020

A new class of enzymes discovered: a non-heme oxidase produces medium-chain 1-alkenes

scientific article published on 16 February 2015

A versatile esterase from Bacillus subtilis: cloning, expression, characterization, and its application in biocatalysis.

scientific article

Accelerated Reaction Engineering of Photo(bio)catalytic Reactions through Parallelization with an Open‐Source Photoreactor

scientific article published in 2021

Altering the scissile fatty acid binding site ofCandida antarcticalipase A by protein engineering for the selective hydrolysis of medium chain fatty acids

article

An Enzymatic Toolbox for the Kinetic Resolution of 2-(Pyridin-x-yl)but-3-yn-2-ols and Tertiary Cyanohydrins

article

Arylmalonate decarboxylase-a highly selective bacterial biocatalyst with unknown function.

scientific article

Biocatalytic synthesis of optically active tertiary alcohols

scientific article published on 21 June 2011

Characterization of a novel esterase isolated from intertidal flat metagenome and its tertiary alcohols synthesis

article

Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants

scientific article published on 12 March 2019

Cloning and characterization of a new delta-specific l-leucine dioxygenase from Anabaena variabilis

scientific article published on 04 August 2018

Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries

scientific article

Complete Inversion of Enantioselectivity towards Acetylated Tertiary Alcohols by a Double Mutant of aBacillus Subtilis Esterase

article

Creation of a Lipase Highly Selective for trans Fatty Acids by Protein Engineering

article

CryoEM analysis of small plant biocatalysts at sub-2 Å resolution

scientific article published on 01 January 2022

Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase

scientific article published on 27 March 2019

Draft Genome Sequence of Bordetella bronchiseptica KU1201, the First Isolation Source of Arylmalonate Decarboxylase.

scientific article

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

article

Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases

scientific article

Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance

article

Engineering the promiscuous racemase activity of an arylmalonate decarboxylase

scientific article published on 09 November 2010

Enzymatic synthesis of optically active tertiary alcohols: expanding the biocatalysis toolbox.

scientific article published on March 2008

Erratum to: The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity

scholarly article published in Applied Microbiology and Biotechnology

Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

scientific article

Gerichtete Evolution und rationales Design. Maßgeschneiderte Enzyme

Ground-State Destabilization by Active-Site Hydrophobicity Controls the Selectivity of a Cofactor-Free Decarboxylase

scientific article published on 12 November 2020

Highly Enantioselective Synthesis of Arylaliphatic Tertiary Alcohols using Mutants of an Esterase fromBacillus subtilis

article

Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis

scientific article published on 19 February 2007

Highly stable protein immobilization via maleimido-thiol chemistry to monitor enzymatic activity

scientific article published on 01 May 2018

Hydrogen-Driven Cofactor Regeneration for Stereoselective Whole-Cell C=C Bond Reduction in Cupriavidus necator

scientific article published on 29 April 2019

Hydrolase-catalyzed stereoselective preparation of protected α,α-dialkyl-α-hydroxycarboxylic acids

Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols

scientific article

Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases

scientific article published on 3 February 2016

Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining

Improvement of the Process Stability of Arylmalonate Decarboxylase by Immobilization for Biocatalytic Profen Synthesis.

scientific article published on 16 March 2017

Internal Illumination to Overcome the Cell Density Limitation in the Scale-up of Whole-Cell Photobiocatalysis

scientific article published on 06 July 2021

Light-driven Enzymatic Decarboxylation

scientific article published on 22 May 2016

One-step enzyme extraction and immobilization for biocatalysis applications

article by Karim Engelmark Cassimjee et al published April 2011 in Biotechnology Journal

Overcoming the Incompatibility Challenge in Chemoenzymatic and Multi-Catalytic Cascade Reactions.

scientific article published on 6 September 2017

Photobiocatalytic decarboxylation for olefin synthesis.

scientific article

Photobiocatalytic synthesis of chiral secondary fatty alcohols from renewable unsaturated fatty acids

scientific article published on 07 May 2020

Photosynthetic production of enantioselective biocatalysts

scientific article

Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis

scientific article published on 05 February 2020

Practical Considerations Regarding the Choice of the Best High-Throughput Assay.

scientific article published in January 2018

Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols

article

Protein engineering and discovery of lipases

article

Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols

scientific article published on 16 July 2011

Raman Micro-Spectroscopic Evidence for the Metabolism of a Tyrosine Kinase Inhibitor, Neratinib, in Cancer Cells.

scientific article published on 12 April 2018

Rational Protein Design of Paenibacillus barcinonensis Esterase EstA for Kinetic Resolution of Tertiary Alcohols

article

Recombinant Cyanobacteria for the Asymmetric Reduction of C=C Bonds Fueled by the Biocatalytic Oxidation of Water

scientific article published on 29 March 2016

STD-NMR-Based Protein Engineering of the Unique Arylpropionate-Racemase AMDase G74C.

scientific article

Sequence-Based Screening for Rare Enzymes: New Insights into the World of AMDases Reveal a Conserved Motif and 58 Novel Enzymes Clustering in Eight Distinct Families.

scientific article published on 25 August 2016

The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering

scientific article published on August 2010

The role of the GGGX motif in determining the activity and enantioselectivity of pig liver esterase towards tertiary alcohols

article

The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity

article

Understanding Promiscuous Amidase Activity of an Esterase from Bacillus subtilis

article

List of works by Robert Kourist

A Multi-Enzymatic Cascade Reaction for the Stereoselective Production of γ-Oxyfunctionalyzed Amino Acids.

A One-Pot Cascade Reaction Combining an Encapsulated Decarboxylase with a Metathesis Catalyst for the Synthesis of Bio-Based Antioxidants

A Reconstructed Common Ancestor of the Fatty Acid Photo-decarboxylase Clade Shows Photo-decarboxylation Activity and Increased Thermostability

A Structural View on the Stereospecificity of Plant Borneol-Type Dehydrogenases

A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts

A combined experimental and modelling approach for the Weimberg pathway optimisation

A new class of enzymes discovered: a non-heme oxidase produces medium-chain 1-alkenes

A versatile esterase from Bacillus subtilis: cloning, expression, characterization, and its application in biocatalysis.

Accelerated Reaction Engineering of Photo(bio)catalytic Reactions through Parallelization with an Open‐Source Photoreactor

Altering the scissile fatty acid binding site ofCandida antarcticalipase A by protein engineering for the selective hydrolysis of medium chain fatty acids

An Enzymatic Toolbox for the Kinetic Resolution of 2-(Pyridin-x-yl)but-3-yn-2-ols and Tertiary Cyanohydrins

Arylmalonate decarboxylase-a highly selective bacterial biocatalyst with unknown function.

Biocatalytic synthesis of optically active tertiary alcohols

Characterization of a novel esterase isolated from intertidal flat metagenome and its tertiary alcohols synthesis

Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants

Cloning and characterization of a new delta-specific l-leucine dioxygenase from Anabaena variabilis

Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries

Complete Inversion of Enantioselectivity towards Acetylated Tertiary Alcohols by a Double Mutant of aBacillus Subtilis Esterase

Creation of a Lipase Highly Selective for trans Fatty Acids by Protein Engineering

CryoEM analysis of small plant biocatalysts at sub-2 Å resolution

Discovery of three novel sesquiterpene synthases from Streptomyces chartreusis NRRL 3882 and crystal structure of an α-eudesmol synthase

Draft Genome Sequence of Bordetella bronchiseptica KU1201, the First Isolation Source of Arylmalonate Decarboxylase.

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases

Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance

Engineering the promiscuous racemase activity of an arylmalonate decarboxylase

Enzymatic synthesis of optically active tertiary alcohols: expanding the biocatalysis toolbox.

Erratum to: The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity

Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Gerichtete Evolution und rationales Design. Maßgeschneiderte Enzyme

Ground-State Destabilization by Active-Site Hydrophobicity Controls the Selectivity of a Cofactor-Free Decarboxylase

Highly Enantioselective Synthesis of Arylaliphatic Tertiary Alcohols using Mutants of an Esterase fromBacillus subtilis

Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis

Highly stable protein immobilization via maleimido-thiol chemistry to monitor enzymatic activity

Hydrogen-Driven Cofactor Regeneration for Stereoselective Whole-Cell C=C Bond Reduction in Cupriavidus necator

Hydrolase-catalyzed stereoselective preparation of protected α,α-dialkyl-α-hydroxycarboxylic acids

Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols

Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases

Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining

Improvement of the Process Stability of Arylmalonate Decarboxylase by Immobilization for Biocatalytic Profen Synthesis.

Internal Illumination to Overcome the Cell Density Limitation in the Scale-up of Whole-Cell Photobiocatalysis

Light-driven Enzymatic Decarboxylation

One-step enzyme extraction and immobilization for biocatalysis applications

Overcoming the Incompatibility Challenge in Chemoenzymatic and Multi-Catalytic Cascade Reactions.

Photobiocatalytic decarboxylation for olefin synthesis.

Photobiocatalytic synthesis of chiral secondary fatty alcohols from renewable unsaturated fatty acids

Photosynthetic production of enantioselective biocatalysts

Plasma-Driven in Situ Production of Hydrogen Peroxide for Biocatalysis

Practical Considerations Regarding the Choice of the Best High-Throughput Assay.

Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols

Protein engineering and discovery of lipases

Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols

Raman Micro-Spectroscopic Evidence for the Metabolism of a Tyrosine Kinase Inhibitor, Neratinib, in Cancer Cells.

Rational Protein Design of Paenibacillus barcinonensis Esterase EstA for Kinetic Resolution of Tertiary Alcohols

Recombinant Cyanobacteria for the Asymmetric Reduction of C=C Bonds Fueled by the Biocatalytic Oxidation of Water

STD-NMR-Based Protein Engineering of the Unique Arylpropionate-Racemase AMDase G74C.

Sequence-Based Screening for Rare Enzymes: New Insights into the World of AMDases Reveal a Conserved Motif and 58 Novel Enzymes Clustering in Eight Distinct Families.

The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering

The role of the GGGX motif in determining the activity and enantioselectivity of pig liver esterase towards tertiary alcohols

The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity

Understanding Promiscuous Amidase Activity of an Esterase from Bacillus subtilis