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List of works by Renate Kunert

A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples

scientific article

A novel strategy for quantitative isoform detection directly performed from culture supernatant.

scientific article published on 9 June 2006

A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits

scientific article published on 18 January 2008

A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1.

scientific article published on December 2001

A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells

scientific article

A study on the temperature dependency and time course of the cold capture antibody secretion assay

scientific article

Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.

scientific article published on 27 August 2014

Advances in recombinant antibody manufacturing

scientific article

Analysis of changes during subclone development and ageing of human antibody-producing heterohybridoma cells by northern blot and flow cytometry.

scientific article published on January 1999

Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides

scientific article

Anti-human immunodeficiency virus type 1 (HIV-1) antibodies 2F5 and 4E10 require surprisingly few crucial residues in the membrane-proximal external region of glycoprotein gp41 to neutralize HIV-1.

scientific article published on January 2005

Anti-idiotypic antibody Ab2/3H6 mimics the epitope of the neutralizing anti-HIV-1 monoclonal antibody 2F5

article

Anti-neuroblastoma effect of ch14.18 antibody produced in CHO cells is mediated by NK-cells in mice.

scientific article published on 7 April 2005

Antibody domain exchange is an immunological solution to carbohydrate cluster recognition

scientific article

Antibody humanization by molecular dynamics simulations-in-silico guided selection of critical backmutations

scientific article published on 8 January 2016

Applicability of different fluorescent dyes for isoform quantification on linear IPG gels

scientific article published on 01 June 2007

Benchmarking of commercially available CHO cell culture media for antibody production.

scientific article

Binding of the 2F5 monoclonal antibody to native and fusion-intermediate forms of human immunodeficiency virus type 1 gp41: implications for fusion-inducing conformational changes.

scientific article published on March 2004

Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells.

scientific article published on 15 June 2006

Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts Favor Either mAb Production or Biomass Synthesis

scientific article published on 11 June 2018

Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression.

scientific article published on 02 April 2016

CHO-recombinant human growth hormone as a protease sensitive reporter protein.

scientific article published on 25 April 2009

Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies.

scientific article

Ch14.18 antibody produced in CHO cells in relapsed or refractory Stage 4 neuroblastoma patients: a SIOPEN Phase 1 study.

scientific article published on 31 May 2013

Changes during subclone development and ageing of human antibody-producing recombinant CHO cells

scientific article published on April 1999

Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters.

scientific article published on 30 December 2006

Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12.

scientific article

Characterization of molecular features, antigen-binding, and in vitro properties of IgG and IgM variants of 4E10, an anti-HIV type 1 neutralizing monoclonal antibody.

scientific article published in July 2004

Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression

scientific article published in January 2017

Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodies

scientific article

Cross-Neutralizing Human Monoclonal Anti-HIV-1 Antibody 2F5: Preparation and Crystallographic Analysis of the Free and Epitope-Complexed Forms of its F ab Fragment

article

Crystal structure of the complex between the F(ab)' fragment of the cross-neutralizing anti-HIV-1 antibody 2F5 and the F(ab) fragment of its anti-idiotypic antibody 3H6

scientific article

Differential gene expression of a feed-spiked super-producing CHO cell line

article

Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line

scientific article

Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12.

scientific article

Engineering of Pichia pastoris for improved production of antibody fragments.

scientific article published in June 2006

Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting

scientific article

Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

scientific article published on 11 March 2014

Evaluation of the potency of the anti-idiotypic antibody Ab2/3H6 mimicking gp41 as an HIV-1 vaccine in a rabbit prime/boost study

scientific article

Exploration of BAC versus plasmid expression vectors in recombinant CHO cells.

scientific article published on 19 October 2012

Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis

article by Andreas Loos et al published April 2011 in Plant Physiology

Fc-glycosylation influences Fcγ receptor binding and cell-mediated anti-HIV activity of monoclonal antibody 2G12.

scientific article published on November 2010

Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension cultures.

scientific article published on 27 February 2007

Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients.

scientific article

Glycan profile of CHO derived IgM purified by highly efficient single step affinity chromatography.

scientific article

Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth

scientific article published on 9 December 2011

Heterologous protein production using euchromatin-containing expression vectors in mammalian cells

scientific article published on 14 May 2015

High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris

article

Humanization strategies for an anti-idiotypic antibody mimicking HIV-1 gp41 ⬇️

scientific article published on October 30, 2010

Identification of bottlenecks in antibody expression using targeted gene integration.

scientific article

Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation

scientific article

Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH.

scientific article published on July 2006

IgM characterization directly performed in crude culture supernatants by a new simple electrophoretic method.

scientific article published on 20 May 2010

Impact of temperature and pH on recombinant human IgM quality attributes and productivity

scientific article published on 07 January 2019

Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis.

scientific article

Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile

scientific article published on 28 May 2009

Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

scientific article published on 14 February 2007

In planta protein sialylation through overexpression of the respective mammalian pathway

scientific article published on 20 March 2010

In search of expression bottlenecks in recombinant CHO cell lines--a case study.

scientific article published on 21 February 2014

Inefficient CAR-proximal signaling blunts antigen sensitivity

scientific article published on 06 July 2020

Influence of cell culture media and feed supplements on cell metabolism and quality of IgG produced in CHO-K1, CHO-S, and CHO-DG44.

scientific article published on 14 December 2015

Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis.

scientific article published on 23 December 2010

Inhibition of human immunodeficiency virus type 1 entry in cells expressing gp41-derived peptides

scientific article published on January 2004

Inhibition of in vivo HIV infection in humanized mice by gene therapy of human hematopoietic stem cells with a lentiviral vector encoding a broadly neutralizing anti-HIV antibody

scientific article

Interaction of anti-HIV type 1 antibody 2F5 with phospholipid bilayers and its relevance for the mechanism of virus neutralization

scientific article published on 15 January 2011

Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM

scientific article published on 5 March 2015

Lessons learned from merging wet lab experiments with molecular simulation to improve mAb humanization

scientific article published on 01 July 2018

Membrane association and epitope recognition by HIV-1 neutralizing anti-gp41 2F5 and 4E10 antibodies.

scientific article

Molecular Characterization of Five Neutralizing Anti-HIV Type 1 Antibodies: Identification of Nonconventional D Segments in the Human Monoclonal Antibodies 2G12 and 2F5

article

Molecular Simulations to Rationalize Humanized Ab2/3H6 Activity

Multiple reaction monitoring targeted LC-MS analysis of potential cell death marker proteins for increased bioprocess control

scientific article published on 02 April 2018

Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for cellular engineering

scientific article

Nomenclature of humanized mAbs: Early concepts, current challenges and future perspectives

scholarly article by Patrick Mayrhofer & Renate Kunert published 23 July 2018 in Human Antibodies

Novel bifunctional single-chain variable antibody fragments to enhance virolysis by complement: generation and proof-of-concept

scientific article published on 12 January 2014

Novel human renal proximal tubular cell line for the production of complex proteins.

scientific article published on 16 February 2014

One-Step Triplex High-Resolution Melting Analysis for Rapid Identification and Simultaneous Subtyping of Frequently Isolated Salmonella Serovars ⬇️

scientific article published on February 17, 2012

Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins

scientific article published on 27 January 2014

Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors

scientific article published on August 2006

Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells

scientific article published in August 2006

Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern

scientific article published in September 2007

Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana

article

Proteomic differences in recombinant CHO cells producing two similar antibody fragments.

scientific article published on 23 February 2016

Rapid development of clone-specific, high-performing perfusion media from established feed supplements

scientific article published on 11 November 2019

Recent advances in recombinant protein production: BAC-based expression vectors, the bigger the better.

scientific article published on 2 July 2013

Recognition and blocking of HIV-1 gp41 pre-transmembrane sequence by monoclonal 4E10 antibody in a Raft-like membrane environment

scientific article published on 18 October 2006

Recognition of membrane-bound fusion-peptide/MPER complexes by the HIV-1 neutralizing 2F5 antibody: implications for anti-2F5 immunogenicity

scientific article

Recombinant IgA production: single step affinity purification using camelid ligands and product characterization.

scientific article published on April 2012

Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans.

scientific article published on 3 November 2007

Screening for improved cell performance: selection of subclones with altered production kinetics or improved stability by cell sorting

scientific article

Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection.

scientific article published on July 2009

Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles

scientific article published on 13 July 2007

Stable overexpression of miR-17 enhances recombinant protein production of CHO cells

scientific article published on 8 February 2014

Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA ⬇️

scientific article published on January 1, 2012

Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5.

scientific article published on 4 September 2007

The broadly neutralizing anti-human immunodeficiency virus type 1 4E10 monoclonal antibody is better adapted to membrane-bound epitope recognition and blocking than 2F5.

scientific article published on 2 July 2008

The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5.

scientific article published on March 2004

Topological transformation of liposomes by a membrane-affecting domain of recombinant human erythropoietin

scientific article published on March 2010

Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysis.

scientific article published on July 2008

Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties

scientific article published on 12 March 2020

Upstream and downstream processing of recombinant IgA.

scientific article published on 26 September 2014

Validated method for quantification of genetically modified organisms in samples of maize flour.

scientific article published in February 2006

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