List of works - Gary J. Lye

A Multidisciplinary Approach Toward the Rapid and Preparative-Scale Biocatalytic Synthesis of Chiral Amino Alcohols: A Concise Transketolase-/ω-Transaminase-Mediated Synthesis of (2S,3S)-2-Aminopentane-1,3-diol

A novel microscale crossflow device for the rapid evaluation of microfiltration processes

A systematic approach to the large-scale production of protein crystals

scientific article published on 01 May 2000

Accelerated design of bioconversion processes using automated microscale processing techniques

scientific article

An automated microscale platform for evaluation and optimization of oxidative bioconversion processes

scientific article published on 5 January 2012

An ultra scale-down method to investigate monoclonal antibody processing during tangential flow filtration (TFF) using ultrafiltration membranes

scientific article published on 04 January 2019

Antibiotic purification from fermentation broths by counter-current chromatography: analysis of product purity and yield trade-offs.

scientific article published on 9 October 2004

Application of bipolar electrodialysis to E. coli fermentation for simultaneous acetate removal and pH control

scientific article published on 11 April 2010

Application of in situ product-removal techniques to biocatalytic processes.

scientific article

Better Biocatalytic Processes Faster: New Tools for the Implementation of Biocatalysis in Organic Synthesis

Centrifugal partition chromatography in a biorefinery context: Separation of monosaccharides from hydrolysed sugar beet pulp.

scientific article published on 6 August 2015

Characterization of lentiviral vector production using microwell suspension cultures of HEK293T-derived producer cells

scientific article published on April 2013

Creation of an ultra scale-down bioreactor mimic for rapid development of lignocellulosic enzymatic hydrolysis processes

scientific article

Design and characterization of a microfluidic packed bed system for protein breakthrough and dynamic binding capacity determination

scientific article published in January 2009

Design and characterization of a prototype enzyme microreactor: quantification of immobilized transketolase kinetics.

scientific article

Design and parallelisation of a miniature photobioreactor platform for microalgal culture evaluation and optimisation

article

Directed evolution of biocatalytic processes

scientific article

Engineering characterisation of a shaken, single-use photobioreactor for early stage microalgae cultivation using Chlorella sorokiniana

scientific article published on 28 September 2014

Engineering characterisation of a single well from 24-well and 96-well microtitre plates

Enhanced recombinant protein synthesis in batch and fed-batchEscherichia colifermentation based on removal of inhibitory acetate by electrodialysis

Enzymatic ketone reductions with co-factor recycling: Improved reactions with ionic liquid co-solvents

Evaluation of anthrax vaccine production by Bacillus anthracis Sterne 34F2 in stirred suspension culture using a miniature bioreactor: A useful scale-down tool for studies on fermentations at high containment

article

Extraction of lysozyme and ribonuclease-a using reverse micelles: Limits to protein solubilization.

scientific article published in September 1995

Fed-batch operation of an industrial cell culture process in shaken microwells

scientific article published on 17 September 2009

Functionalised tetrahydrofuran fragments from carbohydrates or sugar beet pulp biomass

article by Laure Benhamou et al published 2019 in Green Chemistry

High-throughput measurement of protein stability in microtiter plates

scientific article published in March 2005

High-throughput screening and process optimisation

Immobilised enzyme microreactor for screening of multi-step bioconversions: Characterisation of a de novo transketolase-ω-transaminase pathway to synthesise chiral amino alcohols

scientific article published on 22 July 2011

In-situ product removal to enhance the yield of biocatalytic reactions with competing equilibria:?-glucosidase catalysed synthesis of disaccharides

Lipase catalysed resolution of the Lotrafiban intermediate 2,3,4,5-tetrahydro-4-methyl-3-oxo-1H-1,4-benzodiazepine-2-acetic acid methyl ester in ionic liquids: comparison to the industrial t-butanol process

Lowering oxygen tension enhances the differentiation of mouse embryonic stem cells into neuronal cells

scientific article

Mathematical model of a three phase partitioning bioreactor for conversion of ketones using whole cells

Mechanical stability of immobilized biocatalysts (CLECs) in dilute agitated suspensions

scientific article published in January 2002

Microfluidic Chromatography for Early Stage Evaluation of Biopharmaceutical Binding and Separation Conditions

Microfluidic multi-input reactor for biocatalytic synthesis using transketolase

scientific article

Microscale bioprocess optimisation

scientific article published on 3 November 2006

Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines

scientific article published on 01 May 2014

Microwell engineering characterization for mammalian cell culture process development

scientific article

Modeling the performance of pilot-scale countercurrent chromatography: Scale-up predictions and experimental verification of erythromycin separation

scientific article published on 01 March 2003

Modelling surface aeration rates in shaken microtitre plates using dimensionless groups

Non-α-hydroxylated aldehydes with evolved transketolase enzymes

scientific article published on 05 February 2010

On the fluid dynamics of a laboratory scale single-use stirred bioreactor

scientific article

One-pot synthesis of amino-alcohols using a de-novo transketolase and beta-alanine: pyruvate transaminase pathway in Escherichia coli.

scientific article published in February 2007

Optimisation and evaluation of a generic microplate-based HPLC screen for transketolase activity

scientific article

Oxygen-controlled automated neural differentiation of mouse embryonic stem cells

scientific article published on March 2013

Potential of sugar beet vinasse as a feedstock for biocatalyst production within an integrated biorefinery context

article by Nurashikin Suhaili et al published 28 November 2018 in Journal of Chemical Technology & Biotechnology

Quantification and prediction of jet macro-mixing times in static microwell plates

Quantification of kinetics for enzyme-catalysed reactions: implications for diffusional limitations at the 10 ml scale.

scientific article published on 26 January 2008

Quantification of power consumption and oxygen transfer characteristics of a stirred miniature bioreactor for predictive fermentation scale-up.

scientific article published in August 2008

Reactor operation and scale-up of whole cell Baeyer-Villiger catalyzed lactone synthesis

scientific article published on September 2002

Reproducible culture and differentiation of mouse embryonic stem cells using an automated microwell platform

scientific article published on August 2013

Scale-down prediction of industrial scale pleated membrane cartridge performance

scientific article published on 22 December 2010

Scale-up of Escherichia coli growth and recombinant protein expression conditions from microwell to laboratory and pilot scale based on matched k(L)a.

scientific article published in April 2008

The bioreduction of a β-tetralone to its corresponding alcohol by the yeastTrichosporon capitatumMY1890 and bacteriumRhodococcus erythropolisMA7213 in a range of ionic liquids

The full spectrum of physiological oxygen tensions and step-changes in oxygen tension affects the neural differentiation of mouse embryonic stem cells

scientific article published on 07 September 2011

The impact of manual processing on the expansion and directed differentiation of embryonic stem cells

scientific article

The protein fraction from wheat-based dried distiller's grain with solubles (DDGS): extraction and valorization

scientific article

The use of microscale processing technologies for quantification of biocatalytic Baeyer-Villiger oxidation kinetics

scientific article published on October 2002

Thermal profiling for parallel on-line monitoring of biomass growth in miniature stirred bioreactors.

scientific article published on 16 April 2008

UCL biochemical engineering.

scientific article

Use of microwells to investigate the effect of quorum sensing on growth and antigen production in Bacillus anthracis Sterne 34F2

scientific article published on 29 September 2011

Use of operating windows in the assessment of integrated robotic systems for the measurement of bioprocess kinetics

scientific article published in January 2005

List of works by Gary J. Lye

A Multidisciplinary Approach Toward the Rapid and Preparative-Scale Biocatalytic Synthesis of Chiral Amino Alcohols: A Concise Transketolase-/ω-Transaminase-Mediated Synthesis of (2S,3S)-2-Aminopentane-1,3-diol

A novel microscale crossflow device for the rapid evaluation of microfiltration processes

A systematic approach to the large-scale production of protein crystals

Accelerated design of bioconversion processes using automated microscale processing techniques

An automated microscale platform for evaluation and optimization of oxidative bioconversion processes

An ultra scale-down method to investigate monoclonal antibody processing during tangential flow filtration (TFF) using ultrafiltration membranes

Antibiotic purification from fermentation broths by counter-current chromatography: analysis of product purity and yield trade-offs.

Application of bipolar electrodialysis to E. coli fermentation for simultaneous acetate removal and pH control

Application of in situ product-removal techniques to biocatalytic processes.

Better Biocatalytic Processes Faster: New Tools for the Implementation of Biocatalysis in Organic Synthesis

Centrifugal partition chromatography in a biorefinery context: Separation of monosaccharides from hydrolysed sugar beet pulp.

Characterization of lentiviral vector production using microwell suspension cultures of HEK293T-derived producer cells

Creation of an ultra scale-down bioreactor mimic for rapid development of lignocellulosic enzymatic hydrolysis processes

Design and characterization of a microfluidic packed bed system for protein breakthrough and dynamic binding capacity determination

Design and characterization of a prototype enzyme microreactor: quantification of immobilized transketolase kinetics.

Design and parallelisation of a miniature photobioreactor platform for microalgal culture evaluation and optimisation

Directed evolution of biocatalytic processes

Engineering characterisation of a shaken, single-use photobioreactor for early stage microalgae cultivation using Chlorella sorokiniana

Engineering characterisation of a single well from 24-well and 96-well microtitre plates

Enhanced recombinant protein synthesis in batch and fed-batchEscherichia colifermentation based on removal of inhibitory acetate by electrodialysis

Enzymatic ketone reductions with co-factor recycling: Improved reactions with ionic liquid co-solvents

Evaluation of anthrax vaccine production by Bacillus anthracis Sterne 34F2 in stirred suspension culture using a miniature bioreactor: A useful scale-down tool for studies on fermentations at high containment

Extraction of lysozyme and ribonuclease-a using reverse micelles: Limits to protein solubilization.

Fed-batch operation of an industrial cell culture process in shaken microwells

Functionalised tetrahydrofuran fragments from carbohydrates or sugar beet pulp biomass

High-throughput measurement of protein stability in microtiter plates

High-throughput screening and process optimisation

Immobilised enzyme microreactor for screening of multi-step bioconversions: Characterisation of a de novo transketolase-ω-transaminase pathway to synthesise chiral amino alcohols

In-situ product removal to enhance the yield of biocatalytic reactions with competing equilibria:?-glucosidase catalysed synthesis of disaccharides

Lipase catalysed resolution of the Lotrafiban intermediate 2,3,4,5-tetrahydro-4-methyl-3-oxo-1H-1,4-benzodiazepine-2-acetic acid methyl ester in ionic liquids: comparison to the industrial t-butanol process

Lowering oxygen tension enhances the differentiation of mouse embryonic stem cells into neuronal cells

Mathematical model of a three phase partitioning bioreactor for conversion of ketones using whole cells

Mechanical stability of immobilized biocatalysts (CLECs) in dilute agitated suspensions

Microfluidic Chromatography for Early Stage Evaluation of Biopharmaceutical Binding and Separation Conditions

Microfluidic multi-input reactor for biocatalytic synthesis using transketolase

Microscale bioprocess optimisation

Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines

Microwell engineering characterization for mammalian cell culture process development

Modeling the performance of pilot-scale countercurrent chromatography: Scale-up predictions and experimental verification of erythromycin separation

Modelling surface aeration rates in shaken microtitre plates using dimensionless groups

Non-α-hydroxylated aldehydes with evolved transketolase enzymes

On the fluid dynamics of a laboratory scale single-use stirred bioreactor

One-pot synthesis of amino-alcohols using a de-novo transketolase and beta-alanine: pyruvate transaminase pathway in Escherichia coli.

Optimisation and evaluation of a generic microplate-based HPLC screen for transketolase activity

Oxygen-controlled automated neural differentiation of mouse embryonic stem cells

Potential of sugar beet vinasse as a feedstock for biocatalyst production within an integrated biorefinery context

Quantification and prediction of jet macro-mixing times in static microwell plates

Quantification of kinetics for enzyme-catalysed reactions: implications for diffusional limitations at the 10 ml scale.

Quantification of power consumption and oxygen transfer characteristics of a stirred miniature bioreactor for predictive fermentation scale-up.

Reactor operation and scale-up of whole cell Baeyer-Villiger catalyzed lactone synthesis

Reproducible culture and differentiation of mouse embryonic stem cells using an automated microwell platform

Scale-down prediction of industrial scale pleated membrane cartridge performance

Scale-up of Escherichia coli growth and recombinant protein expression conditions from microwell to laboratory and pilot scale based on matched k(L)a.

The bioreduction of a β-tetralone to its corresponding alcohol by the yeastTrichosporon capitatumMY1890 and bacteriumRhodococcus erythropolisMA7213 in a range of ionic liquids

The full spectrum of physiological oxygen tensions and step-changes in oxygen tension affects the neural differentiation of mouse embryonic stem cells

The impact of manual processing on the expansion and directed differentiation of embryonic stem cells

The protein fraction from wheat-based dried distiller's grain with solubles (DDGS): extraction and valorization

The use of microscale processing technologies for quantification of biocatalytic Baeyer-Villiger oxidation kinetics

Thermal profiling for parallel on-line monitoring of biomass growth in miniature stirred bioreactors.

UCL biochemical engineering.

Use of microwells to investigate the effect of quorum sensing on growth and antigen production in Bacillus anthracis Sterne 34F2

Use of operating windows in the assessment of integrated robotic systems for the measurement of bioprocess kinetics