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List of works by Zbyněk Prokop

A Haloalkane Dehalogenase from a Marine Microbial Consortium Possessing Exceptionally Broad Substrate Specificity.

scientific article published on 3 November 2017

An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation

scientific article published on 31 January 2020

Bioavailability and mobility of organic contaminants in soil: new three-step ecotoxicological evaluation.

scientific article

Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58

scientific article

Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.

scientific article published on 05 June 2009

CalFitter: a web server for analysis of protein thermal denaturation data.

scientific article published in July 2018

Catalytic Cycle of Haloalkane Dehalogenases Toward Unnatural Substrates Explored by Computational Modeling

scientific article published on 11 July 2017

Catalytic mechanism of the maloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26.

scientific article published in September 2003

Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases

scientific article

Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2.

scientific article published on 26 December 2017

Computer-assisted engineering of the synthetic pathway for biodegradation of a toxic persistent pollutant.

scientific article published on 17 December 2013

Conformational changes allow processing of bulky substrates by a haloalkane dehalogenase with a small and buried active site

scientific article published on 20 July 2018

Controlled Oil/Water Partitioning of Hydrophobic Substrates Extending the Bioanalytical Applications of Droplet-Based Microfluidics

scientific article published on 10 July 2019

Degradation of beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26

scientific article

Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips ⬇️

scientific article published on 23 September 2018

Determination of capsidiol in tobacco cells culture by HPLC.

scientific article published on July 2010

Development of Fluorescent Assay for Monitoring of Dehalogenase Activity

scientific article published on 28 August 2018

Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear β-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites.

scientific article published on 23 March 2017

Discovery of Novel Haloalkane Dehalogenase Inhibitors.

scientific article published on 15 January 2016

Dynamics and hydration explain failed functional transformation in dehalogenase design

scientific article

Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

scientific article

Enzyme Tunnels and Gates As Relevant Targets in Drug Design.

scientific article

Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard

scientific article

Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway

scientific article published on 21 December 2015

Expansion of access tunnels and active-site cavities influence activity of haloalkane dehalogenases in organic cosolvents

scientific article

Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating.

scientific article published on 24 November 2017

Exploring the structure and activity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26: evidence for product- and water-mediated inhibition

scientific article

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

scientific article published on 3 November 2015

FireProt: web server for automated design of thermostable proteins

scientific article published on 26 April 2017

Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application

scientific article published on 8 December 2015

Halide-stabilizing residues of haloalkane dehalogenases studied by quantum mechanic calculations and site-directed mutagenesis

scientific article

Haloalkane Dehalogenases From Marine Organisms

scientific article published in 2018

Haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26: X-ray crystallographic studies of dehalogenation of brominated substrates

scientific article

Haloalkane dehalogenases: biotechnological applications.

scientific article published on 11 September 2012

Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane

scientific article published on 13 May 2014

Impact of the access tunnel engineering on catalysis is strictly ligand-specific

scientific article published on 25 February 2018

Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets.

scientific article published on 12 December 2014

Kinetics of binding of fluorescent ligands to enzymes with engineered access tunnels.

scientific article

Maximizing the efficiency of multienzyme process by stoichiometry optimization.

scientific article published on 5 August 2014

Mechanism-based discovery of novel substrates of haloalkane dehalogenases using in silico screening

scientific article published on 30 December 2014

Metagenome-derived haloalkane dehalogenases with novel catalytic properties

scientific article

Microscopic monitoring provides information on structure and properties during biocatalyst immobilization

scientific article published on 16 April 2014

Mobility and toxicity of metals in sandy sediments deposited on land

scientific article published in January 2003

Mobility, bioavailability, and toxic effects of cadmium in soil samples.

scientific article published in February 2003

Modification of activity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26 by engineering of its entrance tunnel.

scientific article published on 2 October 2003

Molecular Gating of an Engineered Enzyme Captured in Real Time

scientific article published on 3 December 2018

Novel LinA type 3 δ-hexachlorocyclohexane dehydrochlorinase.

scientific article published on 21 August 2015

Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil.

scientific article

Pathways and mechanisms for product release in the engineered haloalkane dehalogenases explored using classical and random acceleration molecular dynamics simulations.

scientific article

Perspectives: biotechnology in Czech Republic, the past and the future.

scientific article published on May 2006

Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer.

scientific article published on 5 June 2017

Quantitative structure–activity relationships for toxicity and genotoxicity of halogenated aliphatic compounds: Wing spot test of Drosophila melanogaster

scientific article published in February 2007

Reconstruction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB

scientific journal article

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

scientific article

Regio- and Enantioselective Sequential Dehalogenation of rac-1,3-Dibromobutane by Haloalkane Dehalogenase LinB.

scientific article published on 22 June 2016

Release of halide ions from the buried active site of the haloalkane dehalogenase LinB revealed by stopped-flow fluorescence analysis and free energy calculations

scientific article

Sensitive operation of enzyme-based biodevices by advanced signal processing.

scientific article published on 18 June 2018

Sphingobium baderi sp. nov., isolated from a hexachlorocyclohexane dump site.

scientific article

Sphingobium czechense sp. nov., isolated from a hexachlorocyclohexane dump site.

scientific article

Stepwise enhancement of catalytic performance of haloalkane dehalogenase LinB towards β-hexachlorocyclohexane.

scientific article

Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature ⬇️

scientific article published on June 27, 2011

Structural and functional analysis of a novel haloalkane dehalogenase with two halide-binding sites

scientific article

Substrate inhibition by the blockage of product release and its control by tunnel engineering

scientific article published on 11 January 2021

The effect of a unique halide-stabilizing residue on the catalytic properties of haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58.

scientific article

The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: reaction mechanism and molecular evolution

scientific article published on 28 September 2006

Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-Ray Crystallography and Microcalorimetry

scientific article

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