List of works - Michael J. McLeish

A bulky hydrophobic residue is not required to maintain the V-conformation of enzyme-bound thiamin diphosphate

scientific article

A peptide corresponding to the N-terminal 13 residues of T4 lysozyme forms an alpha-helix

scientific article published on 01 January 1993

Aspartate aminotransferase catalyzed oxygen exchange with solvent from oxygen-18-enriched alpha-ketoglutarate: evidence for slow exchange of enzyme-bound water

scientific article published in May 1989

CD and NMR determination of the solution structure of a peptide corresponding to T4 lysozyme residues 38-51

scientific article published on 01 July 1995

Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae.

scientific article

Characterization of benzaldehyde lyase from Pseudomonas fluorescens: A versatile enzyme for asymmetric C-C bond formation

scientific article published on 31 October 2006

Chemical modification of PABA synthase.

scientific article published in August 1991

Computational characterization of enzyme-bound thiamin diphosphate reveals a surprisingly stable tricyclic state: implications for catalysis

scientific article published on 16 January 2019

Conformation of a peptide corresponding to T4 lysozyme residues 59-81 by NMR and CD spectroscopy

scientific article published on 01 September 1994

Conformational analysis of LYS(11-36), a peptide derived from the beta-sheet region of T4 lysozyme, in TFE and SDS

scientific article published on 01 September 1997

Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase

scientific article published on 9 October 2006

Determination of (R)-(+)- and (S)-(-)-isomers of thiopentone in plasma by chiral high-performance liquid chromatography

scientific article published in January 1996

Elucidation of the chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: defining internal equilibria among tautomeric and ionization states

scientific article published on 23 August 2007

Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida.

scientific article published in June 2005

Exploring the role of the active site cysteine in human muscle creatine kinase

scientific article

Expression of Torpedo californica creatine kinase in Escherichia coli and purification from inclusion bodies

scientific article published in October 2002

Extended reaction scope of thiamine diphosphate dependent cyclohexane-1,2-dione hydrolase: from C-C bond cleavage to C-C bond ligation

scientific article

Fragment-based screening by X-ray crystallography, MS and isothermal titration calorimetry to identify PNMT (phenylethanolamine N-methyltransferase) inhibitors

scientific article

Gas chromatographic-mass spectroscopic characterisation of the psychotomimetic indolealkylamines and their in vivo metabolites

scientific article published on 01 November 1987

Getting the adrenaline going: crystal structure of the adrenaline-synthesizing enzyme PNMT

scientific article

Heterogeneity of Escherichia coli-expressed human muscle creatine kinase.

scientific article published in July 2006

Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633.

scientific article

Identification of charge transfer transitions related to thiamin-bound intermediates on enzymes provides a plethora of signatures useful in mechanistic studies

scientific article published on 26 March 2014

Identification of initiation sites for T4 lysozyme folding using CD and NMR spectroscopy of peptide fragments.

scientific article

Inhibitors of phenylethanolamine N-methyltransferase that are predicted to penetrate the blood-brain barrier: design, synthesis, and evaluation of 3-fluoromethyl-7-(N-substituted aminosulfonyl)-1,2,3,4-tetrahydroisoquinolines that possess low [...]

scientific article published in August 2004

Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201.

scientific article

Isoleucine 69 and valine 325 form a specificity pocket in human muscle creatine kinase

scientific article published in November 2004

Kinetic and pH studies on human phenylethanolamine N-methyltransferase

scientific article published on 07 September 2013

Loop movement and catalysis in creatine kinase.

scientific article published in April 2005

Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature family.

scientific article

Mechanism-based inactivation of thioredoxin reductase from Plasmodium falciparum by Mannich bases. Implication for cytotoxicity

scientific article published on November 2003

Molecular recognition of physiological substrate noradrenaline by the adrenaline-synthesizing enzyme PNMT and factors influencing its methyltransferase activity

scientific article

Molecular recognition of sub-micromolar inhibitors by the epinephrine-synthesizing enzyme phenylethanolamine N-methyltransferase

scientific article

Perturbation of the Monomer–Monomer Interfaces of the Benzoylformate Decarboxylase Tetramer

scientific article

Pharmacokinetics of thiopental and pentobarbital enantiomers after intravenous administration of racemic thiopental

scientific article published in September 1996

Phenylethanolamine N-methyltransferase inhibition: re-evaluation of kinetic data.

scientific article

Phenylethanolamine N-methyltransferase kinetics: bovine versus recombinant human enzyme.

scientific article published in June 2001

Phosphonodifluoropyruvate is a mechanism-based inhibitor of phosphonopyruvate decarboxylase from Bacteroides fragilis.

scientific article published on 13 June 2017

Physical, kinetic and spectrophotometric studies of a NAD(P)-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633.

scientific article published on 2 May 2008

Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism

scholarly article

Specific inhibitors of Plasmodium falciparum thioredoxin reductase as potential antimalarial agents.

scientific article published on 3 February 2006

Specificity and mechanism of mandelamide hydrolase catalysis

scientific article published on 24 January 2017

Spectroscopic detection of transient thiamin diphosphate-bound intermediates on benzoylformate decarboxylase

scientific article published on 01 November 2000

Structural Relationship between the Active Sites of β-Lactam-Recognizing and Amidase Signature Enzymes: Convergent Evolution? ⬇️

scientific article published on October 26, 2010

Structure-Based Drug Design of Bisubstrate Inhibitors of Phenylethanolamine N-Methyltransferase Possessing Low Nanomolar Affinity at Both Substrate Binding Domains1

scientific article published on 04 November 2020

Studies on the conformational properties of CP-10(42-55), the hinge region of CP-10, using circular dichroism and RP-HPLC

scientific article published on 01 June 2000

Substrate specificity in thiamin diphosphate-dependent decarboxylases.

scientific article published on 30 December 2011

The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complex

scientific article

The Crystal Structure of Benzoylformate Decarboxylase at 1.6 Å Resolution: Diversity of Catalytic Residues in Thiamin Diphosphate-Dependent Enzymes, ⬇️

scientific article published on July 14, 1998

The cloning and over-expression of PABA synthase in E. coli.

scientific article

The kinetic characterization and X-ray structure of a putative benzoylformate decarboxylase from M. smegmatis highlights the difficulties in the functional annotation of ThDP-dependent enzymes

scientific article

The reaction mechanism of phenylethanolamine N-methyltransferase: a density functional theory study.

scientific article published on 03 September 2009

Time-dependent inactivation of human phenylethanolamine N-methyltransferase by 7-isothiocyanatotetrahydroisoquinoline

scientific article published on 10 January 2009

Using directed evolution to probe the substrate specificity of mandelamide hydrolase

scientific article

Using site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes.

scientific article published on 23 August 2013

List of works by Michael J. McLeish

A bulky hydrophobic residue is not required to maintain the V-conformation of enzyme-bound thiamin diphosphate

A peptide corresponding to the N-terminal 13 residues of T4 lysozyme forms an alpha-helix

Aspartate aminotransferase catalyzed oxygen exchange with solvent from oxygen-18-enriched alpha-ketoglutarate: evidence for slow exchange of enzyme-bound water

CD and NMR determination of the solution structure of a peptide corresponding to T4 lysozyme residues 38-51

Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae.

Characterization of benzaldehyde lyase from Pseudomonas fluorescens: A versatile enzyme for asymmetric C-C bond formation

Chemical modification of PABA synthase.

Computational characterization of enzyme-bound thiamin diphosphate reveals a surprisingly stable tricyclic state: implications for catalysis

Conformation of a peptide corresponding to T4 lysozyme residues 59-81 by NMR and CD spectroscopy

Conformational analysis of LYS(11-36), a peptide derived from the beta-sheet region of T4 lysozyme, in TFE and SDS

Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase

Determination of (R)-(+)- and (S)-(-)-isomers of thiopentone in plasma by chiral high-performance liquid chromatography

Elucidation of the chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: defining internal equilibria among tautomeric and ionization states

Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida.

Exploring the role of the active site cysteine in human muscle creatine kinase

Expression of Torpedo californica creatine kinase in Escherichia coli and purification from inclusion bodies

Extended reaction scope of thiamine diphosphate dependent cyclohexane-1,2-dione hydrolase: from C-C bond cleavage to C-C bond ligation

Fragment-based screening by X-ray crystallography, MS and isothermal titration calorimetry to identify PNMT (phenylethanolamine N-methyltransferase) inhibitors

Gas chromatographic-mass spectroscopic characterisation of the psychotomimetic indolealkylamines and their in vivo metabolites

Getting the adrenaline going: crystal structure of the adrenaline-synthesizing enzyme PNMT

Heterogeneity of Escherichia coli-expressed human muscle creatine kinase.

Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633.

Identification of charge transfer transitions related to thiamin-bound intermediates on enzymes provides a plethora of signatures useful in mechanistic studies

Identification of initiation sites for T4 lysozyme folding using CD and NMR spectroscopy of peptide fragments.

Inhibitors of phenylethanolamine N-methyltransferase that are predicted to penetrate the blood-brain barrier: design, synthesis, and evaluation of 3-fluoromethyl-7-(N-substituted aminosulfonyl)-1,2,3,4-tetrahydroisoquinolines that possess low [...]

Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201.

Isoleucine 69 and valine 325 form a specificity pocket in human muscle creatine kinase

Kinetic and pH studies on human phenylethanolamine N-methyltransferase

Loop movement and catalysis in creatine kinase.

Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature family.

Mechanism-based inactivation of thioredoxin reductase from Plasmodium falciparum by Mannich bases. Implication for cytotoxicity

Molecular recognition of physiological substrate noradrenaline by the adrenaline-synthesizing enzyme PNMT and factors influencing its methyltransferase activity

Molecular recognition of sub-micromolar inhibitors by the epinephrine-synthesizing enzyme phenylethanolamine N-methyltransferase

Perturbation of the Monomer–Monomer Interfaces of the Benzoylformate Decarboxylase Tetramer

Pharmacokinetics of thiopental and pentobarbital enantiomers after intravenous administration of racemic thiopental

Phenylethanolamine N-methyltransferase inhibition: re-evaluation of kinetic data.

Phenylethanolamine N-methyltransferase kinetics: bovine versus recombinant human enzyme.

Phosphonodifluoropyruvate is a mechanism-based inhibitor of phosphonopyruvate decarboxylase from Bacteroides fragilis.

Physical, kinetic and spectrophotometric studies of a NAD(P)-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633.

Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism

Specific inhibitors of Plasmodium falciparum thioredoxin reductase as potential antimalarial agents.

Specificity and mechanism of mandelamide hydrolase catalysis

Spectroscopic detection of transient thiamin diphosphate-bound intermediates on benzoylformate decarboxylase

Structural Relationship between the Active Sites of β-Lactam-Recognizing and Amidase Signature Enzymes: Convergent Evolution? ⬇️

Structure-Based Drug Design of Bisubstrate Inhibitors of Phenylethanolamine N-Methyltransferase Possessing Low Nanomolar Affinity at Both Substrate Binding Domains1

Studies on the conformational properties of CP-10(42-55), the hinge region of CP-10, using circular dichroism and RP-HPLC

Substrate specificity in thiamin diphosphate-dependent decarboxylases.

The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complex

The Crystal Structure of Benzoylformate Decarboxylase at 1.6 Å Resolution: Diversity of Catalytic Residues in Thiamin Diphosphate-Dependent Enzymes, ⬇️

The cloning and over-expression of PABA synthase in E. coli.

The kinetic characterization and X-ray structure of a putative benzoylformate decarboxylase from M. smegmatis highlights the difficulties in the functional annotation of ThDP-dependent enzymes

The reaction mechanism of phenylethanolamine N-methyltransferase: a density functional theory study.

Time-dependent inactivation of human phenylethanolamine N-methyltransferase by 7-isothiocyanatotetrahydroisoquinoline

Using directed evolution to probe the substrate specificity of mandelamide hydrolase

Using site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes.