List of works - Dmitry Dolgikh

'Molten-globule' state accumulates in carbonic anhydrase folding

scientific article published on 01 January 1984

A cytochrome c mutant with high electron transfer and antioxidant activities but devoid of apoptogenic effect

scientific article published on March 2002

A new approach to artificial and modified proteins: theory-based design, synthesis in a cell-free system and fast testing of structural properties by radiolabels.

scientific article published in August 1994

A new hybrid protein for production of recombinant bacteriorhodopsin in Escherichia coli

scientific article published on 02 April 2010

Alpha-Lactalbumin: compact state with fluctuating tertiary structure?

scientific article published on 01 December 1981

Alternative scaffold proteins

scientific article

Amyloidogenic properties of the artificial protein albebetin and its biologically active derivatives. The role of electrostatic interactions in fibril formation

scientific article published on 01 March 2006

An efficient method for expression in Escherichia coli and purification of the extracellular ligand binding domain of the human TGFbeta type II receptor.

scientific article published on 06 May 2010

Anion-selective channelrhodopsin expressed in neuronal cell culture and in vivo in murine brain: Light-induced inhibition of generation of action potentials

scientific article published in November 2015

Aspartate-histidine interaction in the retinal schiff base counterion of the light-driven proton pump of Exiguobacterium sibiricum.

scientific article

Bacterial production and refolding from inclusion bodies of a "weak" toxin, a disulfide rich protein.

scientific article

Biochemical characterization of human enteropeptidase light chain.

scientific article published in February 2006

Can grafting of an octapeptide improve the structure of a de novo protein?

scientific article published on 01 March 1998

Cell surface display of cold-active esterase EstPc with the use of a new autotransporter from Psychrobacter cryohalolentis K5(T).

scientific article published on 25 September 2014

Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors.

scientific article published on 22 June 2016

Characterization of a cold-active lipase from Psychrobacter cryohalolentis K5(T) and its deletion mutants.

scientific article published in April 2013

Combination of TRAIL with bortezomib shifted apoptotic signaling from DR4 to DR5 death receptor by selective internalization and degradation of DR4.

scientific article

Compact state of a protein molecule with pronounced small-scale mobility: bovine alpha-lactalbumin

scientific article published on 01 January 1985

Comparative analysis of proapoptotic activity of cytochrome c mutants in living cells.

scientific article published on August 2005

Complex kinetics of the electron transfer between the photoactive redox label TUPS and the heme of cytochrome c.

scientific article published in November 2005

Construction of TNF-binding proteins by grafting hypervariable regions of F10 antibody on human fibronectin domain scaffold.

scientific article

Cytotoxicity of albebetin oligomers depends on cross-beta-sheet formation

scientific article published on 7 April 2006

De novo design, synthesis and study of albebetin, a polypeptide with a predetermined three-dimensional structure. Probing the structure at the nanogram level.

scientific article

Dissecting structural basis of the unique substrate selectivity of human enteropeptidase catalytic subunit

scientific article published on 01 January 2012

ESR - a retinal protein with unusual properties from Exiguobacterium sibiricum

scientific article

Early events during folding of wild-type staphylococcal nuclease and a single-tryptophan variant studied by ultrarapid mixing.

scientific article

Electrogenic steps of light-driven proton transport in ESR, a retinal protein from Exiguobacterium sibiricum.

scientific article published on 12 August 2016

Expression and chaperone-assisted refolding of a new cold-active lipase from Psychrobacter cryohalolentis K5(T).

scientific article published on 26 July 2013

Expression of the envelope antigen F1 of Yersinia pestis is mediated by the product of caf1M gene having homology with the chaperone protein PapD of Escherichia coli

scientific article published on July 1991

Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli.

scientific article published in September 2003

Femtosecond spectroscopic study of photochromic reactions of bacteriorhodopsin and visual rhodopsin

scientific article published on 2 October 2016

Fibrillation of carrier protein albebetin and its biologically active constructs. Multiple oligomeric intermediates and pathways.

scientific article published in August 2004

Folding of circular permutants with decreased contact order: general trend balanced by protein stability 1 1Edited by A. R. Fersht

GFP–Margatoxin, a Genetically Encoded Fluorescent Ligand to Probe Affinity of Kv1.3 Channel Blockers

scientific article published in 2022

Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1) Is a Selective Allosteric Antagonist of α7 Nicotinic Acetylcholine Receptor

scientific article

Human neuromodulator SLURP-1: bacterial expression, binding to muscle-type nicotinic acetylcholine receptor, secondary structure, and conformational heterogeneity in solution.

scientific article published in February 2013

Interaction of three-finger proteins from snake venoms and from mammalian brain with the cys-loop receptors and their models.

scientific article published on May 2016

Lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: comparison with detergent micelles, bicelles and liposomes

scientific article published on 26 October 2011

Lipolytic enzymes of microorganisms from permafrost cryopegs.

scientific article

Lynx1 and Aβ1-42 bind competitively to multiple nicotinic acetylcholine receptor subtypes

scientific article published on 17 June 2016

Multiple Mutations in the Non-Ordered Red Ω-Loop Enhance the Membrane-Permeabilizing and Peroxidase-like Activity of Cytochrome <i>c</i>

scientific article published on 04 May 2022

Mutations Enhancing Selectivity of Antitumor Cytokine TRAIL to DR5 Receptor Increase Its Cytotoxicity against Tumor Cells

scientific article published on August 2015

Natively unfolded human prothymosin alpha adopts partially folded collapsed conformation at acidic pH.

scientific article

Neurotoxins from snake venoms and α-conotoxin ImI inhibit functionally active ionotropic γ-aminobutyric acid (GABA) receptors

scientific article published on 28 July 2015

New monoclonal antibodies to the Ebola virus glycoprotein: Identification and analysis of the amino acid sequence of the variable domains

scientific article

New strategy for high-level expression and purification of biologically active monomeric TGF-β1/C77S in Escherichia coli.

scientific article published in February 2015

Noncooperative temperature melting of a globular protein without specific tertiary structure: acid form of bovine carbonic anhydrase B

scientific article published on 01 October 1985

Novel mutants of human tumor necrosis factor with dominant-negative properties.

scientific article published on December 2010

Overexpression in Escherichia coli and purification of human fibroblast growth factor (FGF-2).

scientific article published in February 2009

Photocycle of Exiguobacterium sibiricum rhodopsin characterized by low-temperature trapping in the IR and time-resolved studies in the visible

scientific article published on 10 June 2013

Proapoptotic activity of cytochrome c in living cells: effect of K72 substitutions and species differences.

scientific article published on 19 April 2008

Production of anti TNF-α antibodies in eukaryotic cells using different combinations of vectors carrying heavy and light chains

scientific article published on 18 June 2014

Production of biologically active human myelocytokines in plants

scientific article

Role of individual lysine residues of horse cytochrome c in the formation of reactive complexes with components of the respiratory chain

scientific article published in January 2010

S6 permutein shows that the unusual target topology is not responsible for the absence of rigid tertiary structure in de novo protein albebetin.

scientific article

Secreted Isoform of Human Lynx1 (SLURP-2): Spatial Structure and Pharmacology of Interactions with Different Types of Acetylcholine Receptors

scientific article

Site-directed mutagenesis of cytochrome c: reactions with respiratory chain components and superoxide radical.

scientific article published in June 2009

Solid-state electron transport via cytochrome c depends on electronic coupling to electrodes and across the protein.

scientific article published on 31 March 2014

Spatial structure and dimer--monomer equilibrium of the ErbB3 transmembrane domain in DPC micelles

scientific article

Strategy for improvement of enteropeptidase efficiency in tag removal processes.

scientific article published on 15 April 2011

Structural Insight into Specificity of Interactions between Nonconventional Three-finger Weak Toxin from Naja kaouthia (WTX) and Muscarinic Acetylcholine Receptors

scientific article published on 4 August 2015

Structural insights into the proton pumping by unusual proteorhodopsin from nonmarine bacteria

scientific article

Structure and stability of recombinant protein depend on the extra N-terminal methionine residue: S6 permutein from direct and fusion expression systems

scientific article published on 01 July 1999

Synthetic proteins with antiviral properties of alpha-interferons

scientific article published in May 2003

The development and optimization of coupled cell-free expression system for production of the transmembrane domain of the receptor tyrosine kinase ErbB3

scientific article

The in vitro production of three-finger neurotoxins from snake venoms with a high abundance of disulfide bonds. Problems and their solutions

scientific article published in March 2010

The structure of ribonuclease in solution does not differ from its crystalline structure

scientific article published on 01 April 1978

Towards universal approach for bacterial production of three-finger Ly6/uPAR proteins: Case study of cytotoxin I from cobra N. oxiana.

scientific article published on October 2016

Water-soluble LYNX1 residues important for interaction with muscle-type and/or neuronal nicotinic receptors.

scientific article

[An artificial protein, possessing biological activity of HL-60 human promyelocytic leukemia differentiation factor]

scientific article published on 01 January 2003

[Bacterial expression of water-soluble domain of Lynx1, endogenic neuromodulator of human nicotinic acetylcholine receptors].

scientific article

[Neutralizing Monoclonal and Chimeric Antibodies to Human IFN-γ].

scientific article

[Production of antibodies to aflatoxins]

scientific article published on 01 January 2010

pH-induced equilibrium unfolding of apomyoglobin: substitutions at conserved Trp14 and Met131 and non-conserved Val17 positions

scientific article published in June 2008

List of works by Dmitry Dolgikh

'Molten-globule' state accumulates in carbonic anhydrase folding

A cytochrome c mutant with high electron transfer and antioxidant activities but devoid of apoptogenic effect

A new approach to artificial and modified proteins: theory-based design, synthesis in a cell-free system and fast testing of structural properties by radiolabels.

A new hybrid protein for production of recombinant bacteriorhodopsin in Escherichia coli

Alpha-Lactalbumin: compact state with fluctuating tertiary structure?

Alternative scaffold proteins

Amyloidogenic properties of the artificial protein albebetin and its biologically active derivatives. The role of electrostatic interactions in fibril formation

An efficient method for expression in Escherichia coli and purification of the extracellular ligand binding domain of the human TGFbeta type II receptor.

Anion-selective channelrhodopsin expressed in neuronal cell culture and in vivo in murine brain: Light-induced inhibition of generation of action potentials

Aspartate-histidine interaction in the retinal schiff base counterion of the light-driven proton pump of Exiguobacterium sibiricum.

Bacterial production and refolding from inclusion bodies of a "weak" toxin, a disulfide rich protein.

Biochemical characterization of human enteropeptidase light chain.

Can grafting of an octapeptide improve the structure of a de novo protein?

Cell surface display of cold-active esterase EstPc with the use of a new autotransporter from Psychrobacter cryohalolentis K5(T).

Central loop of non-conventional toxin WTX from Naja kaouthia is important for interaction with nicotinic acetylcholine receptors.

Characterization of a cold-active lipase from Psychrobacter cryohalolentis K5(T) and its deletion mutants.

Combination of TRAIL with bortezomib shifted apoptotic signaling from DR4 to DR5 death receptor by selective internalization and degradation of DR4.

Compact state of a protein molecule with pronounced small-scale mobility: bovine alpha-lactalbumin

Comparative analysis of proapoptotic activity of cytochrome c mutants in living cells.

Complex kinetics of the electron transfer between the photoactive redox label TUPS and the heme of cytochrome c.

Construction of TNF-binding proteins by grafting hypervariable regions of F10 antibody on human fibronectin domain scaffold.

Cytotoxicity of albebetin oligomers depends on cross-beta-sheet formation

De novo design, synthesis and study of albebetin, a polypeptide with a predetermined three-dimensional structure. Probing the structure at the nanogram level.

Dissecting structural basis of the unique substrate selectivity of human enteropeptidase catalytic subunit

ESR - a retinal protein with unusual properties from Exiguobacterium sibiricum

Early events during folding of wild-type staphylococcal nuclease and a single-tryptophan variant studied by ultrarapid mixing.

Electrogenic steps of light-driven proton transport in ESR, a retinal protein from Exiguobacterium sibiricum.

Expression and chaperone-assisted refolding of a new cold-active lipase from Psychrobacter cryohalolentis K5(T).

Expression of the envelope antigen F1 of Yersinia pestis is mediated by the product of caf1M gene having homology with the chaperone protein PapD of Escherichia coli

Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli.

Femtosecond spectroscopic study of photochromic reactions of bacteriorhodopsin and visual rhodopsin

Fibrillation of carrier protein albebetin and its biologically active constructs. Multiple oligomeric intermediates and pathways.

Folding of circular permutants with decreased contact order: general trend balanced by protein stability 1 1Edited by A. R. Fersht

GFP–Margatoxin, a Genetically Encoded Fluorescent Ligand to Probe Affinity of Kv1.3 Channel Blockers

Human Secreted Ly-6/uPAR Related Protein-1 (SLURP-1) Is a Selective Allosteric Antagonist of α7 Nicotinic Acetylcholine Receptor

Human neuromodulator SLURP-1: bacterial expression, binding to muscle-type nicotinic acetylcholine receptor, secondary structure, and conformational heterogeneity in solution.

Interaction of three-finger proteins from snake venoms and from mammalian brain with the cys-loop receptors and their models.

Lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: comparison with detergent micelles, bicelles and liposomes

Lipolytic enzymes of microorganisms from permafrost cryopegs.

Lynx1 and Aβ1-42 bind competitively to multiple nicotinic acetylcholine receptor subtypes

Multiple Mutations in the Non-Ordered Red Ω-Loop Enhance the Membrane-Permeabilizing and Peroxidase-like Activity of Cytochrome <i>c</i>

Mutations Enhancing Selectivity of Antitumor Cytokine TRAIL to DR5 Receptor Increase Its Cytotoxicity against Tumor Cells

Natively unfolded human prothymosin alpha adopts partially folded collapsed conformation at acidic pH.

Neurotoxins from snake venoms and α-conotoxin ImI inhibit functionally active ionotropic γ-aminobutyric acid (GABA) receptors

New monoclonal antibodies to the Ebola virus glycoprotein: Identification and analysis of the amino acid sequence of the variable domains

New strategy for high-level expression and purification of biologically active monomeric TGF-β1/C77S in Escherichia coli.

Noncooperative temperature melting of a globular protein without specific tertiary structure: acid form of bovine carbonic anhydrase B

Novel mutants of human tumor necrosis factor with dominant-negative properties.

Overexpression in Escherichia coli and purification of human fibroblast growth factor (FGF-2).

Photocycle of Exiguobacterium sibiricum rhodopsin characterized by low-temperature trapping in the IR and time-resolved studies in the visible

Proapoptotic activity of cytochrome c in living cells: effect of K72 substitutions and species differences.

Production of anti TNF-α antibodies in eukaryotic cells using different combinations of vectors carrying heavy and light chains

Production of biologically active human myelocytokines in plants

Role of individual lysine residues of horse cytochrome c in the formation of reactive complexes with components of the respiratory chain

S6 permutein shows that the unusual target topology is not responsible for the absence of rigid tertiary structure in de novo protein albebetin.

Secreted Isoform of Human Lynx1 (SLURP-2): Spatial Structure and Pharmacology of Interactions with Different Types of Acetylcholine Receptors

Site-directed mutagenesis of cytochrome c: reactions with respiratory chain components and superoxide radical.

Solid-state electron transport via cytochrome c depends on electronic coupling to electrodes and across the protein.

Spatial structure and dimer--monomer equilibrium of the ErbB3 transmembrane domain in DPC micelles

Strategy for improvement of enteropeptidase efficiency in tag removal processes.

Structural Insight into Specificity of Interactions between Nonconventional Three-finger Weak Toxin from Naja kaouthia (WTX) and Muscarinic Acetylcholine Receptors

Structural insights into the proton pumping by unusual proteorhodopsin from nonmarine bacteria

Structure and stability of recombinant protein depend on the extra N-terminal methionine residue: S6 permutein from direct and fusion expression systems

Synthetic proteins with antiviral properties of alpha-interferons

The development and optimization of coupled cell-free expression system for production of the transmembrane domain of the receptor tyrosine kinase ErbB3

The in vitro production of three-finger neurotoxins from snake venoms with a high abundance of disulfide bonds. Problems and their solutions

The structure of ribonuclease in solution does not differ from its crystalline structure

Towards universal approach for bacterial production of three-finger Ly6/uPAR proteins: Case study of cytotoxin I from cobra N. oxiana.

Water-soluble LYNX1 residues important for interaction with muscle-type and/or neuronal nicotinic receptors.

[An artificial protein, possessing biological activity of HL-60 human promyelocytic leukemia differentiation factor]

[Bacterial expression of water-soluble domain of Lynx1, endogenic neuromodulator of human nicotinic acetylcholine receptors].

[Neutralizing Monoclonal and Chimeric Antibodies to Human IFN-γ].

[Production of antibodies to aflatoxins]

pH-induced equilibrium unfolding of apomyoglobin: substitutions at conserved Trp14 and Met131 and non-conserved Val17 positions