List of works - Frank Bernhard

A Disulfide Bridge Network within the Soluble Periplasmic Domain Determines Structure and Function of the Outer Membrane Protein RCSF

scientific article

A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii.

scientific article published on May 1993

A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.

scientific article

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.

scientific article

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

article

Analysis of engineered multifunctional peptide synthetases. Enzymatic characterization of surfactin synthetase domains in hybrid bimodular systems

scientific article

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology

scientific article (publication date: 9 January 2017)

Artificial environments for the co-translational stabilization of cell-free expressed proteins

scientific article

Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain

scientific article published in September 2004

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing

scientific article published on 22 May 2015

Cell free expression and functional reconstitution of eukaryotic drug transporters

scientific journal article

Cell-Free Expression of G-Protein-Coupled Receptors

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

scientific article

Cell-free expression and assembly of ATP synthase.

scientific article

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination

scientific article

Cell-free expression and stable isotope labelling strategies for membrane proteins

Cell-free expression as an emerging technique for the large scale production of integral membrane protein

scientific article published on 23 August 2006

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets

scientific article published on December 2014

Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening

scientific article

Cell-free expression profiling of E. coli inner membrane proteins

scientific article

Cell-free expression--making a mark.

scientific article published on 27 April 2013

Cell-free production of G protein-coupled receptors for functional and structural studies.

scientific article published on 23 January 2007

Cell-free production of integral membrane proteins on a preparative scale

scientific article published on January 2007

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

scientific article

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.

scientific article published on 18 August 2012

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

scientific article

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments

scientific article published on 25 February 2016

Co-translational stabilization of insoluble proteins in cell-free expression systems

scientific article

Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins

scientific article published on July 2006

Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment

scientific article published on 18 January 2012

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression

scientific article

Conformational Switches Modulate Protein Interactions in Peptide Antibiotic Synthetases

scientific article published in Science

Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.

scientific article

Cryo-EM structure of cell-free synthesized human histamine 2 receptor/Gs complex in nanodisc environment

scientific article published in 2024

Efficient Strategy for the Rapid Backbone Assignment of Membrane Proteins

scientific article published in 2005

Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system

scientific article

From Gene to Function: Cell-free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs

scientific article published on 24 April 2017

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins

scientific article published on 7 September 2016

Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation

article

Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism ⬇️

scientific article published on 18 January 2012

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification

scientific article

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments

scientific article published on 6 June 2013

Genetic transfer of amylovoran and stewartan synthesis between Erwinia amylovora and Erwinia stewartii

scientific article published on 01 May 1996

High level cell-free expression and specific labeling of integral membrane proteins

scientific article

High-level cell-free production of membrane proteins with nanodiscs.

scientific article

Inhibition of microsomal prostaglandin E2 synthase-1 as a molecular basis for the anti-inflammatory actions of boswellic acids from frankincense ⬇️

scientific article published on January 2011

Insights into Cotranslational Membrane Protein Insertion by Combined LILBID-Mass Spectrometry and NMR Spectroscopy

scientific article published on 17 October 2017

Interaction of the regulator proteins RcsA and RcsB with the promoter of the operon for amylovoran biosynthesis in Erwinia amylovora.

scientific article published on September 1997

Labeling of membrane proteins by cell-free expression

scientific article published on 02 July 2015

Large-scale production of functional membrane proteins

scientific article published on June 2008

Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism

scientific article published in 2018

Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway

scientific article published on 30 November 2015

Membrane Protein Production in E. coli Lysates in Presence of Preassembled Nanodiscs.

scientific article published in January 2017

Membrane protein expression in cell-free systems

scientific article published in January 2010

Membrane protein production in Escherichia coli cell-free lysates

scientific article

Modification of biologically active peptides: production of a novel lipohexapeptide after engineering of Bacillus subtilis surfactin synthetase.

scientific article

Modified lipid and protein dynamics in nanodiscs

scientific article published on 28 December 2012

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor

article published in 2010

Modulation of the Rcs-mediated signal transfer by conformational flexibility.

scientific article published in December 2008

Molecular engineering aspects for the production of new and modified biosurfactants.

scientific article published on January 2010

NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain.

scientific article

Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins

scientific article published on 22 June 2018

Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies

scientific article published on April 2007

Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes

scientific article

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.

scientific article published in January 2007

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes

scientific article

Production of membrane proteins using cell-free expression systems

scientific article published in October 2008

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs

scientific article published on 20 March 2015

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

scientific article

Single-Molecule Force Spectroscopy from Nanodiscs: An Assay to Quantify Folding, Stability, and Interactions of Native Membrane Proteins

scientific article published on 30 December 2011

Solution NMR Structure of Proteorhodopsin

scientific article

Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains.

scientific article published in October 2004

Strategies for the Cell-Free Expression of Membrane Proteins

Structural Analysis of the DNA-binding Domain of the Erwinia amylovora RcsB Protein and Its Interaction with the RcsAB Box ⬇️

scientific article (publication date: 16 May 2003)

Structural Basis for Tail-Anchored Membrane Protein Biogenesis by the Get3-Receptor Complex

scientific article

Structural basis for the selectivity of the external thioesterase of the surfactin synthetase

scientific article

Structural investigation of the C-terminal catalytic fragment of presenilin 1

scientific article

Substrate specificity of hybrid modules from peptide synthetases

scientific article

Systematic optimization of cell-free synthesized human endothelin B receptor folding

scientific article published on 26 January 2018

Systems for the cell-free synthesis of proteins.

scientific article published in January 2012

The E. coli S30 lysate proteome: A prototype for cell-free protein production

scientific article published on 21 September 2017

The large extracellular loop of organic cation transporter 1 influences substrate affinity and is pivotal for oligomerization

scientific journal article

The molecular pharmacology and in vivo activity of 2-(4-chloro-6-(2,3-dimethylphenylamino)pyrimidin-2-ylthio)octanoic acid (YS121), a dual inhibitor of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase

scientific article

The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase.

scientific article published on November 1999

The soluble loop BC region guides, but not dictates, the assembly of the transmembrane cytochrome b6.

scientific article published on 14 December 2017

The synaptic vesicle protein SV31 assembles into a dimer and transports Zn2.

scientific article published on 5 November 2016

Transmembrane segment enhanced labeling as a tool for the backbone assignment of alpha-helical membrane proteins

scholarly article

Triple-resonance methods for complete resonance assignment of aromatic protons and directly bound heteronuclei in histidine and tryptophan residues.

scientific article

List of works by Frank Bernhard

A Disulfide Bridge Network within the Soluble Periplasmic Domain Determines Structure and Function of the Outer Membrane Protein RCSF

A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii.

A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

Analysis of engineered multifunctional peptide synthetases. Enzymatic characterization of surfactin synthetase domains in hybrid bimodular systems

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology

Artificial environments for the co-translational stabilization of cell-free expressed proteins

Assignment of (1)H, (13)C and (15)N resonances of the Escherichia coli YojN histidine-phosphotransferase (HPt) domain

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing

Cell free expression and functional reconstitution of eukaryotic drug transporters

Cell-Free Expression of G-Protein-Coupled Receptors

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

Cell-free expression and assembly of ATP synthase.

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination

Cell-free expression and stable isotope labelling strategies for membrane proteins

Cell-free expression as an emerging technique for the large scale production of integral membrane protein

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets

Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening

Cell-free expression profiling of E. coli inner membrane proteins

Cell-free expression--making a mark.

Cell-free production of G protein-coupled receptors for functional and structural studies.

Cell-free production of integral membrane proteins on a preparative scale

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments

Co-translational stabilization of insoluble proteins in cell-free expression systems

Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins

Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression

Conformational Switches Modulate Protein Interactions in Peptide Antibiotic Synthetases

Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.

Cryo-EM structure of cell-free synthesized human histamine 2 receptor/Gs complex in nanodisc environment

Efficient Strategy for the Rapid Backbone Assignment of Membrane Proteins

Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system

From Gene to Function: Cell-free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins

Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation

Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism ⬇️

Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: implications for the role of the naturally occurring mycolic acid modification

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments

Genetic transfer of amylovoran and stewartan synthesis between Erwinia amylovora and Erwinia stewartii

High level cell-free expression and specific labeling of integral membrane proteins

High-level cell-free production of membrane proteins with nanodiscs.

Inhibition of microsomal prostaglandin E2 synthase-1 as a molecular basis for the anti-inflammatory actions of boswellic acids from frankincense ⬇️

Insights into Cotranslational Membrane Protein Insertion by Combined LILBID-Mass Spectrometry and NMR Spectroscopy

Interaction of the regulator proteins RcsA and RcsB with the promoter of the operon for amylovoran biosynthesis in Erwinia amylovora.

Labeling of membrane proteins by cell-free expression

Large-scale production of functional membrane proteins

Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism

Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway

Membrane Protein Production in E. coli Lysates in Presence of Preassembled Nanodiscs.

Membrane protein expression in cell-free systems

Membrane protein production in Escherichia coli cell-free lysates

Modification of biologically active peptides: production of a novel lipohexapeptide after engineering of Bacillus subtilis surfactin synthetase.

Modified lipid and protein dynamics in nanodiscs

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor

Modulation of the Rcs-mediated signal transfer by conformational flexibility.

Molecular engineering aspects for the production of new and modified biosurfactants.

NMR assignment of 1H, 13C and 15N resonances of the truncated Escherichia coli RcsC (700-949), including the phosphoreceiver domain.

Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins

Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies

Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.

Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes

Production of membrane proteins using cell-free expression systems

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

Single-Molecule Force Spectroscopy from Nanodiscs: An Assay to Quantify Folding, Stability, and Interactions of Native Membrane Proteins

Solution NMR Structure of Proteorhodopsin

Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains.

Strategies for the Cell-Free Expression of Membrane Proteins

Structural Analysis of the DNA-binding Domain of the Erwinia amylovora RcsB Protein and Its Interaction with the RcsAB Box ⬇️

Structural Basis for Tail-Anchored Membrane Protein Biogenesis by the Get3-Receptor Complex

Structural basis for the selectivity of the external thioesterase of the surfactin synthetase

Structural investigation of the C-terminal catalytic fragment of presenilin 1

Substrate specificity of hybrid modules from peptide synthetases

Systematic optimization of cell-free synthesized human endothelin B receptor folding