List of works - Masayasu Mie

A DNA-scaffold platform enhances a multi-enzymatic cycling reaction

scientific article published on 23 January 2018

Activity-based in vitro selection of T4 DNA ligase

scientific article published in October 2005

Application of elastin-based nanoparticles displaying antibody binding domains for a homogeneous immunoassay.

scientific article

Aptamer-based protein detection using a bioluminescent fusion protein.

scientific article

Assembly of zinc finger motif-fused enzymes on a dsDNA scaffold for catalyzing consecutive reactions with a proximity effect.

scientific article published on 13 September 2014

Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe

scientific article published on 06 February 2007

Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity.

scientific article

Cell-surface-localized ATP detection with immobilized firefly luciferase.

scientific article

Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

scientific article published in February 2003

Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides.

scientific article published on 25 July 2014

Combined effect of electrical stimulation and cisplatin in HeLa cell death

scientific article published on 01 June 2004

Construction and use of an electrochemical NO sensor in a cell-based assessing system

scientific article published in April 2004

Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein

scientific article published on 03 January 2019

Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells

scientific article published on 7 June 2016

Construction of a bFGF-tethered extracellular matrix using a coiled-coil helical interaction.

scientific article

Construction of a bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction.

scientific article

Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains.

scientific article published on 20 October 2010

Construction of a multi-functional extracellular matrix protein that increases number of N1E-115 neuroblast cells having neurites.

scientific article

Construction of a thermostable cell adhesion protein for reverse transfection ⬇️

scientific article published on October 8, 2010

Construction of affinity changeable antibody in response to Ca2+.

scientific article

Construction of intramolecular luciferase complementation probe for detecting specific RNA

scientific article published on 17 March 2007

Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells

scientific article published on 03 November 2011

Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells.

scientific article

Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain.

scientific article

Construction of semisynthetic DNA-protein conjugates with Phi X174 Gene-A* protein.

scientific article published in June 2012

Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form.

scientific article

Delivery of antibody-captured proteins into living cells using PTD-fused protein A

scientific article published on 28 June 2006

Delivery of bFGF for Tissue Engineering by Tethering to the ECM.

scientific article published on 11 October 2015

Design and construction of glutamine binding proteins with a self-adhering capability to unmodified hydrophobic surfaces as reagentless fluorescence sensing devices.

scientific article published in December 2003

Design of a thermocontrollable protein complex

scientific article published on 28 August 2007

Design of bFGF-tethered self-assembling extracellular matrix proteins via coiled-coil triple-helix formation.

scientific article published on 9 August 2017

Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes.

scientific article published on 14 November 2016

Designed Short Peptides that Form Amyloid-Like Fibrils in Coassembly with Amyloid β-Peptide (Aβ) Decrease the Toxicity of Aβ to Neuronal PC12 Cells

article

Detection of antigens using a protein-DNA chimera developed by enzymatic covalent bonding with phiX gene A*

scientific article published on 16 May 2012

Detection of bioactive small molecules by fluorescent resonance energy transfer (FRET) in RNA-protein conjugates

scientific article published in December 2009

Development of a Split SNAP-CLIP Double Labeling System for Tracking Proteins Following Dissociation from Protein-Protein Complexes in Living Cells

scientific article published on 22 July 2016

Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase.

scientific article

Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein.

scientific article published on 2 August 2013

Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells.

scientific article

Development of immune cellular biosensing system for assessing chemicals on inducible nitric oxide synthase signaling activator.

scientific article published in September 2003

Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase.

scientific article published on 3 May 2011

Evaluation of small ligand-protein interaction by ligation reaction with DNA-modified ligand.

scientific article published on 25 August 2009

Fabrication of an antibody microwell array with self-adhering antibody binding protein

scientific article published on 17 January 2006

Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation.

scientific article published on 4 April 2017

Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening.

scientific article published on 15 September 2005

Growth Factor Tethering to Protein Nanoparticles via Coiled-Coil Formation for Targeted Drug Delivery

scientific article

Immuno-rolling circle amplification using a multibinding fusion protein.

scientific article

In vitro selection of zinc finger DNA-binding proteins through ribosome display

scientific article

Induction of neural differentiation by electrically stimulated gene expression of NeuroD2.

scientific article published on February 2003

Intracellular delivery of antibodies using TAT fusion protein A ⬇️

scientific article published on October 24, 2003

Method for detection of specific nucleic acids by recombinant protein with fluorescent resonance energy transfer

scientific article

Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein.

scientific article published on July 2008

Novel recombination system using Cre recombinase alpha complementation

scientific article published on 25 May 2007

Osteogenesis coordinated in C3H10T1/2 cells by adipogenesis-dependent BMP-2 expression system.

scientific article published on February 2000

Promotion of angiogenesis by an artificial extracellular matrix protein containing the laminin-1-derived IKVAV sequence.

scientific article published in September 2009

RNA detection using peptide-inserted Renilla luciferase

scientific article published on 01 November 2008

Recombination system based on cre alpha complementation and leucine zipper fusions.

scientific article

Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads.

scientific article published in March 2002

Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX

scientific article published on 14 February 2011

Selection of mRNA 5'-untranslated region sequence with high translation efficiency through ribosome display

scientific article

Systematic screening of the cellular uptake of designed alpha-helix peptides.

scientific article

Targeted delivery using immunoliposomes with a lipid-modified antibody-binding protein.

scientific article

Targeted gene delivery via PEI complexed with an antibody

scientific article published on 11 October 2012

Targeting of EGF-displayed protein nanoparticles with anticancer drugs.

scientific article published on 3 April 2014

The construction of endothelial cellular biosensing system for the control of blood pressure drugs

scientific article published in April 2004

The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein

scientific article published on 01 April 2003

Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system.

scientific article published on 25 February 2015

Transduction of MyoD protein into myoblasts induces myogenic differentiation without addition of protein transduction domain.

scientific article published on 14 March 2009

Transduction of NeuroD2 protein induced neural cell differentiation

scientific journal article

List of works by Masayasu Mie

A DNA-scaffold platform enhances a multi-enzymatic cycling reaction

Activity-based in vitro selection of T4 DNA ligase

Application of elastin-based nanoparticles displaying antibody binding domains for a homogeneous immunoassay.

Aptamer-based protein detection using a bioluminescent fusion protein.

Assembly of zinc finger motif-fused enzymes on a dsDNA scaffold for catalyzing consecutive reactions with a proximity effect.

Assessment of small ligand-protein interactions by electrophoretic mobility shift assay using DNA-modified ligand as a sensing probe

Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity.

Cell-surface-localized ATP detection with immobilized firefly luciferase.

Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides.

Combined effect of electrical stimulation and cisplatin in HeLa cell death

Construction and use of an electrochemical NO sensor in a cell-based assessing system

Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein

Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells

Construction of a bFGF-tethered extracellular matrix using a coiled-coil helical interaction.

Construction of a bFGF-tethered multi-functional extracellular matrix protein through coiled-coil structures for neurite outgrowth induction.

Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains.

Construction of a multi-functional extracellular matrix protein that increases number of N1E-115 neuroblast cells having neurites.

Construction of a thermostable cell adhesion protein for reverse transfection ⬇️

Construction of affinity changeable antibody in response to Ca2+.

Construction of intramolecular luciferase complementation probe for detecting specific RNA

Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells

Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells.

Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain.

Construction of semisynthetic DNA-protein conjugates with Phi X174 Gene-A* protein.

Construction of streptavidin-luciferase fusion protein for ATP sensing with fixed form.

Delivery of antibody-captured proteins into living cells using PTD-fused protein A

Delivery of bFGF for Tissue Engineering by Tethering to the ECM.

Design and construction of glutamine binding proteins with a self-adhering capability to unmodified hydrophobic surfaces as reagentless fluorescence sensing devices.

Design of a thermocontrollable protein complex

Design of bFGF-tethered self-assembling extracellular matrix proteins via coiled-coil triple-helix formation.

Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes.

Designed Short Peptides that Form Amyloid-Like Fibrils in Coassembly with Amyloid β-Peptide (Aβ) Decrease the Toxicity of Aβ to Neuronal PC12 Cells

Detection of antigens using a protein-DNA chimera developed by enzymatic covalent bonding with phiX gene A*

Detection of bioactive small molecules by fluorescent resonance energy transfer (FRET) in RNA-protein conjugates

Development of a Split SNAP-CLIP Double Labeling System for Tracking Proteins Following Dissociation from Protein-Protein Complexes in Living Cells

Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase.

Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein.

Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells.

Development of immune cellular biosensing system for assessing chemicals on inducible nitric oxide synthase signaling activator.

Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase.

Evaluation of small ligand-protein interaction by ligation reaction with DNA-modified ligand.

Fabrication of an antibody microwell array with self-adhering antibody binding protein

Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation.

Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening.

Growth Factor Tethering to Protein Nanoparticles via Coiled-Coil Formation for Targeted Drug Delivery

Immuno-rolling circle amplification using a multibinding fusion protein.

In vitro selection of zinc finger DNA-binding proteins through ribosome display

Induction of neural differentiation by electrically stimulated gene expression of NeuroD2.

Intracellular delivery of antibodies using TAT fusion protein A ⬇️

Method for detection of specific nucleic acids by recombinant protein with fluorescent resonance energy transfer

Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein.

Novel recombination system using Cre recombinase alpha complementation

Osteogenesis coordinated in C3H10T1/2 cells by adipogenesis-dependent BMP-2 expression system.

Promotion of angiogenesis by an artificial extracellular matrix protein containing the laminin-1-derived IKVAV sequence.

RNA detection using peptide-inserted Renilla luciferase

Recombination system based on cre alpha complementation and leucine zipper fusions.

Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads.

Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX

Selection of mRNA 5'-untranslated region sequence with high translation efficiency through ribosome display

Systematic screening of the cellular uptake of designed alpha-helix peptides.

Targeted delivery using immunoliposomes with a lipid-modified antibody-binding protein.

Targeted gene delivery via PEI complexed with an antibody

Targeting of EGF-displayed protein nanoparticles with anticancer drugs.

The construction of endothelial cellular biosensing system for the control of blood pressure drugs

The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein

Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system.

Transduction of MyoD protein into myoblasts induces myogenic differentiation without addition of protein transduction domain.

Transduction of NeuroD2 protein induced neural cell differentiation