List of works - Ralf Kühn

An RNAi-based approach to down-regulate a gene family in vivo

scientific article

CD19-independent instruction of murine marginal zone B-cell development by constitutive Notch2 signaling.

scientific article

Caspase-mediated apoptosis induction in zebrafish cerebellar Purkinje neurons.

scientific article

Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity

scientific article published on 09 September 2019

Characterization of the melanocortin-4-receptor nonsense mutation W16X in vitro and in vivo

scientific article published on 4 October 2011

Chronic CD30 signaling in B cells results in lymphomagenesis by driving the expansion of plasmablasts and B1 cells

scientific article published on 08 April 2019

Conditional RNAi in mice.

scientific article published on 10 August 2010

Constitutive and conditional RNAi transgenesis in mice

scientific article published on 22 December 2010

Control of gene editing by manipulation of DNA repair mechanisms

scientific article published on 3 April 2017

Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos.

scientific article

Csk controls antigen receptor-mediated development and selection of T-lineage cells ⬇️

scientific article published on August 27, 1998

Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome

scientific article published on 26 March 2021

Development of an intein-mediated split-Cas9 system for gene therapy

scientific article

Differential mRNA distribution of components of the ERK/MAPK signalling cascade in the adult mouse brain

scientific article published in January 2007

Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides

scientific article published on 20 February 2013

Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line

scientific article published on 11 October 2016

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

scientific article published on 16 January 2016

Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector

scientific article

Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination

scientific article published on 23 October 2020

FGF/FGFR2 signaling regulates the generation and correct positioning of Bergmann glia cells in the developing mouse cerebellum

scientific article

Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.

scientific article published on March 2017

Gene Editing in One-Cell Embryos by Zinc-Finger and TAL Nucleases.

scientific article published in December 2012

Gene editing and clonal isolation of human induced pluripotent stem cells using CRISPR/Cas9.

scientific article published on 15 May 2017

Gene editing in mouse zygotes using the CRISPR/Cas9 system

scientific article published on 2 March 2017

Gene knockdown in the mouse through RNAi.

scientific article published on January 2010

Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

scientific article

Generating conditional knockout mice

scientific article published on 01 January 2011

Generation of shRNA transgenic mice

scientific article published in January 2009

Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA.

scientific article published on 31 October 2013

Generation of targeted mouse mutants by embryo microinjection of TALENs

scientific article published on 11 January 2014

Genetic mouse models for behavioral analysis through transgenic RNAi technology

scientific article published on 2 June 2008

Genome Editing in Mice Using TALE Nucleases

scientific article published in January 2016

Highly efficient targeted mutagenesis in mice using TALENs

scientific article published on 26 August 2013

Humanized c-Myc mouse

scientific article

In vivo functional requirement of the mouse Ifitm1 gene for germ cell development, interferon mediated immune response and somitogenesis

scientific article

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.

scientific article

Local knockdown of ERK2 in the adult mouse brain via adeno-associated virus-mediated RNA interference.

scientific article published on 04 December 2008

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function

scientific article published on 10 August 2017

MAPK signaling determines anxiety in the juvenile mouse brain but depression-like behavior in adults

scientific article

Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth

scientific article published on 01 June 2022

Modeling disease mutations by gene targeting in one-cell mouse embryos

scientific article

Overview on mouse mutagenesis.

scientific article published on January 2009

Phenotypic annotation of the mouse X chromosome

scientific article

Pink1-deficiency in mice impairs gait, olfaction and serotonergic innervation of the olfactory bulb

scientific article published on 11 January 2012

Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9.

scientific article published on 10 November 2015

Precise CRISPR-Cas-mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells

Reversible and tissue-specific activation of MAP kinase signaling by tamoxifen in Braf(V637)ER(T2) mice.

scientific article

Sall4 isoforms act during proximal-distal and anterior-posterior axis formation in the mouse embryo

scientific article published on September 2008

Target validation in mice by constitutive and conditional RNAi.

scientific article

The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4

scientific article published on 14 March 2019

The functional annotation of mammalian genomes: the challenge of phenotyping

scientific article published on January 2009

List of works by Ralf Kühn

An RNAi-based approach to down-regulate a gene family in vivo

CD19-independent instruction of murine marginal zone B-cell development by constitutive Notch2 signaling.

Caspase-mediated apoptosis induction in zebrafish cerebellar Purkinje neurons.

Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity

Characterization of the melanocortin-4-receptor nonsense mutation W16X in vitro and in vivo

Chronic CD30 signaling in B cells results in lymphomagenesis by driving the expansion of plasmablasts and B1 cells

Conditional RNAi in mice.

Constitutive and conditional RNAi transgenesis in mice

Control of gene editing by manipulation of DNA repair mechanisms

Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos.

Csk controls antigen receptor-mediated development and selection of T-lineage cells ⬇️

Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome

Development of an intein-mediated split-Cas9 system for gene therapy

Differential mRNA distribution of components of the ERK/MAPK signalling cascade in the adult mouse brain

Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides

Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector

Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination

FGF/FGFR2 signaling regulates the generation and correct positioning of Bergmann glia cells in the developing mouse cerebellum

Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.

Gene Editing in One-Cell Embryos by Zinc-Finger and TAL Nucleases.

Gene editing and clonal isolation of human induced pluripotent stem cells using CRISPR/Cas9.

Gene editing in mouse zygotes using the CRISPR/Cas9 system

Gene knockdown in the mouse through RNAi.

Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases

Generating conditional knockout mice

Generation of shRNA transgenic mice

Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA.

Generation of targeted mouse mutants by embryo microinjection of TALENs

Genetic mouse models for behavioral analysis through transgenic RNAi technology

Genome Editing in Mice Using TALE Nucleases

Highly efficient targeted mutagenesis in mice using TALENs

Humanized c-Myc mouse

In vivo functional requirement of the mouse Ifitm1 gene for germ cell development, interferon mediated immune response and somitogenesis

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.

Local knockdown of ERK2 in the adult mouse brain via adeno-associated virus-mediated RNA interference.

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function

MAPK signaling determines anxiety in the juvenile mouse brain but depression-like behavior in adults

Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth

Modeling disease mutations by gene targeting in one-cell mouse embryos

Overview on mouse mutagenesis.

Phenotypic annotation of the mouse X chromosome

Pink1-deficiency in mice impairs gait, olfaction and serotonergic innervation of the olfactory bulb

Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9.

Precise CRISPR-Cas-mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells

Reversible and tissue-specific activation of MAP kinase signaling by tamoxifen in Braf(V637)ER(T2) mice.

Sall4 isoforms act during proximal-distal and anterior-posterior axis formation in the mouse embryo

Target validation in mice by constitutive and conditional RNAi.

The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4

The functional annotation of mammalian genomes: the challenge of phenotyping