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List of works by John R. Engen

A conformational investigation of propeptide binding to the integral membrane protein γ-glutamyl carboxylase using nanodisc hydrogen exchange mass spectrometry

scientific article published on 26 February 2014

A conserved isoleucine maintains the inactive state of Bruton's tyrosine kinase

scientific article

A redox switch regulates the structure and function of anti-apoptotic BFL-1

scientific article published on 13 July 2020

Abl N-terminal cap stabilization of SH3 domain dynamics

scientific article

Accessing the reproducibility and specificity of pepsin and other aspartic proteases

scientific article published on 10 October 2012

Achieving a Graded Immune Response: BTK Adopts a Range of Active/Inactive Conformations Dictated by Multiple Interdomain Contacts

scientific article published on 10 August 2017

Activation loop dynamics determine the different catalytic efficiencies of B cell- and T cell-specific tec kinases.

scientific article

Activation of the Src family kinase Hck without SH3-linker release

scientific article

Active-site inhibitors modulate the dynamic properties of human monoacylglycerol lipase: a hydrogen exchange mass spectrometry study

scientific article published on 08 July 2013

Allosteric control of Ubp6 and the proteasome via a bidirectional switch

scientific article published on 11 February 2022

Allosteric inhibition of antiapoptotic MCL-1.

scientific article

Allosteric interactions between the myristate- and ATP-site of the Abl kinase

scientific article

Allosteric loss-of-function mutations in HIV-1 Nef from a long-term non-progressor

scientific article

Altered dynamics in Lck SH3 upon binding to the LBD1 domain of Herpesvirus saimiri Tip

scientific article

An examination of dynamics crosstalk between SH2 and SH3 domains by hydrogen/deuterium exchange and mass spectrometry

scientific article

Analysis of overlapped and noisy hydrogen/deuterium exchange mass spectra

scientific article published on 10 September 2013

Analysis of protein complexes with hydrogen exchange and mass spectrometry.

scientific article

Analysis of protein conformation and dynamics by hydrogen/deuterium exchange MS.

scientific article published on October 2009

Analytical Aspects of Hydrogen Exchange Mass Spectrometry

scientific article

Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars

scientific article published on 29 June 2012

Antibody mechanics on a membrane-bound HIV segment essential for GP41-targeted viral neutralization

scientific article published on 16 October 2011

Applications of hydrogen/deuterium exchange MS from 2012 to 2014.

scientific article

Aspartic proteinases in Antarctic fish

scientific article published on March 2009

Catalytic site remodelling of the DOT1L methyltransferase by selective inhibitors

scientific article

Chapter 4 Protein Analysis with Hydrogen–Deuterium Exchange Mass Spectrometry

Characterization of Aggregation Propensity of a Human Fc-Fusion Protein Therapeutic by Hydrogen/Deuterium Exchange Mass Spectrometry

scientific article

Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry

article

Characterization of IgG1 conformation and conformational dynamics by hydrogen/deuterium exchange mass spectrometry

scientific article

Chemically Induced Degradation of the Oncogenic Transcription Factor BCL6.

scientific article

Conformational Features of the Full-Length HIV and SIV Nef Proteins Determined by Mass Spectrometry†

article

Conformational Locking upon Cooperative Assembly of Notch Transcription Complexes

scientific article

Conformational analysis of membrane proteins in phospholipid bilayer nanodiscs by hydrogen exchange mass spectrometry

scientific article

Conformational analysis of processivity clamps in solution demonstrates that tertiary structure does not correlate with protein dynamics

scientific article published on 6 March 2014

Conformational analysis of recombinant monoclonal antibodies with hydrogen/deuterium exchange mass spectrometry

scientific article published on January 2013

Conformational differences between arrestin2 and pre-activated mutants as revealed by hydrogen exchange mass spectrometry

scientific article published in August 2005

Conformational disturbance in Abl kinase upon mutation and deregulation

scientific article

Conformational dynamics of the Escherichia coli DNA polymerase manager proteins UmuD and UmuD'.

scientific article

Conformational transition of membrane-associated terminally acylated HIV-1 Nef.

scientific article

Conformational transitions in the membrane scaffold protein of phospholipid bilayer nanodiscs

scientific article

Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data

scientific article published on 01 January 2020

Cumulative deamidations of the major lens protein γS-crystallin increase its aggregation during unfolding and oxidation

scientific article published on 22 July 2020

Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice

scientific article published on 19 August 2019

Determining the site of spin trapping of the equine myoglobin radical by combined use of EPR, electrophoretic purification, and mass spectrometry

scientific article

Developments in Hydrogen/Deuterium Exchange Mass Spectrometry

scientific article published on 28 October 2020

Differential impact of BTK active site inhibitors on the conformational state of full-length BTK

scientific article published on 23 November 2020

Differential sensitivity of Src-family kinases to activation by SH3 domain displacement

scientific article published on 21 August 2014

Dynamic Allostery Mediated by a Conserved Tryptophan in the Tec Family Kinases

scientific article

Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD

scientific article published on 01 March 2018

Dynamic and structural differences between heme oxygenase-1 and -2 are due to differences in their C-terminal regions

scientific article published on 03 April 2019

Dynamics of the Tec-family tyrosine kinase SH3 domains

scientific article published on 25 January 2016

ETD in a traveling wave ion guide at tuned Z-spray ion source conditions allows for site-specific hydrogen/deuterium exchange measurements

scientific article

Effects of Disease-Causing Mutations on the Conformation of Human Apolipoprotein A-I in Model Lipoproteins

scientific article published on 13 July 2018

Effects of HIV-1 Nef on human N-myristoyltransferase 1.

scientific article

Electron transfer control in soluble methane monooxygenase

scientific article published on 24 June 2014

Endocannabinoid enzyme engineering: soluble human thio-monoacylglycerol lipase (sol-S-hMGL).

scientific article

Enhanced SH3/linker interaction overcomes Abl kinase activation by gatekeeper and myristic acid binding pocket mutations and increases sensitivity to small molecule inhibitors

scientific article published on 9 January 2013

Erratum: Corrigendum: Catalytic site remodelling of the DOT1L methyltransferase by selective inhibitors

scientific article published in Nature Communications

Escherichia coli processivity clamp β from DNA polymerase III is dynamic in solution

scientific article published on 10 June 2011

Evidence for increased exposure of the Notch1 metalloprotease cleavage site upon conversion to an activated conformation

scientific article

Extensive Deuterium Back-Exchange in Certain Immobilized Pepsin Columns Used for H/D Exchange Mass Spectrometry

article

False EX1 signatures caused by sample carryover during HX MS analyses

scientific article published on April 2011

Fetal alcohol exposure alters neurosteroid levels in the developing rat brain.

scientific article published in September 2004

Foreword

Functional characterization and conformational analysis of the Herpesvirus saimiri Tip-C484 protein

scientific article published on 16 December 2006

Gas-phase hydrogen/deuterium exchange in a traveling wave ion guide for the examination of protein conformations

scientific article published on December 2009

General structural features that regulate integrin affinity revealed by atypical αVβ8

scientific article published on 02 December 2019

GroEL/ES Chaperonin Modulates the Mechanism and Accelerates the Rate of TIM-Barrel Domain Folding

scientific article

HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck

scientific article published on 15 March 2012

High-speed and high-resolution UPLC separation at zero degrees Celsius

scientific article

Homogeneous Oligomers of Pro-apoptotic BAX Reveal Structural Determinants of Mitochondrial Membrane Permeabilization

scientific article published on 09 June 2020

Hydrocarbon-stitched Peptide Agonists of Glucagon-Like Peptide-1 Receptor

scientific article published on 29 April 2020

Hydrogen Exchange Mass Spectrometry of Proteins at Langmuir Monolayers

scientific article published on 2 July 2015

Hydrogen Exchange Mass Spectrometry of Related Proteins with Divergent Sequences: A Comparative Study of HIV-1 Nef Allelic Variants

scientific article published on 31 March 2016

Hydrogen Exchange Shows Peptide Binding Stabilizes Motions in Hck SH2†

article

Hydrogen deuterium exchange mass spectrometry applied to chaperones and chaperone-assisted protein folding

scientific article published on 24 June 2019

Hydrogen exchange and covalent modification: Focus on biomolecular structure, dynamics, and function. 18th Sanibel Conference on Mass Spectrometry

article

Hydrogen exchange mass spectrometry for the analysis of protein dynamics

scientific article

Hydrogen exchange mass spectrometry: are we out of the quicksand?

scientific article

Hydrogen exchange mass spectrometry: what is it and what can it tell us?

scientific article

Hydrogen exchange-mass spectrometry measures stapled peptide conformational dynamics and predicts pharmacokinetic properties

scientific article

Hydrogen-Deuterium Exchange Mass Spectrometry to Study Protein Complexes

scientific article published on 01 January 2018

Hydrogen/deuterium exchange mass spectrometry applied to IL-23 interaction characteristics: potential impact for therapeutics

scientific article

Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications

scientific article

Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis

scientific article

Identification and localization of slow, natural, cooperative unfolding in the hematopoietic cell kinase SH3 domain by amide hydrogen exchange and mass spectrometry.

scientific article published on November 1997

Identification of a Covalent Molecular Inhibitor of Anti-apoptotic BFL-1 by Disulfide Tethering

scientific article published on 21 April 2020

Identification of a Structural Determinant for Selective Targeting of HDMX

scientific article published on 29 April 2020

Inhibition of Flaviviruses by Targeting a Conserved Pocket on the Viral Envelope Protein

scientific article published on 21 June 2018

Inhibition of Pro-apoptotic BAX by a noncanonical interaction mechanism.

scientific article published on 12 February 2015

Insights into Notch3 activation and inhibition mediated by antibodies directed against its negative regulatory region

scientific article published on 6 June 2013

Investigating monoclonal antibody aggregation using a combination of H/DX-MS and other biophysical measurements

scientific article

Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry.

scientific article

Ion mobility adds an additional dimension to mass spectrometric analysis of solution-phase hydrogen/deuterium exchange

scientific article

Isotope Exchange and Covalent Modification Strategies for Studying Protein Structure and Function

KRAS G12C Drug Development: Discrimination between Switch II Pocket Configurations Using Hydrogen/Deuterium-Exchange Mass Spectrometry.

scientific article published on 25 July 2017

L-canavanine is a time-controlled mechanism-based inhibitor of Pseudomonas aeruginosa arginine deiminase

scientific article published in November 2005

Membrane phospholipid bilayer as a determinant of monoacylglycerol lipase kinetic profile and conformational repertoire

scientific article published on 29 April 2013

Membrane-Associated Conformation of HIV-1 Nef Investigated with Hydrogen Exchange Mass Spectrometry at a Langmuir Monolayer

scientific article published on 2 July 2015

Mitochondrial ClpX activates an essential biosynthetic enzyme through partial unfolding

scientific article published on 24 February 2020

Molecular insight into the conformational dynamics of the Elongin BC complex and its interaction with HIV-1 Vif.

scientific article

Neutron reflectometry study of the conformation of HIV Nef bound to lipid membranes

scientific article

Noncanonical role of the PDZ4 domain of the adaptor protein PDZK1 in the regulation of the hepatic high density lipoprotein receptor scavenger receptor class B, type I (SR-BI).

scientific article published on 17 May 2013

Noncognate DNA damage prevents the formation of the active conformation of the Y-family DNA polymerases DinB and DNA polymerase κ

scientific article published on 11 May 2015

Novel mutant-selective EGFR kinase inhibitors against EGFR T790M

scientific article

On the solution conformation and dynamics of the HIV-1 viral infectivity factor

scientific article

Partial cooperative unfolding in proteins as observed by hydrogen exchange mass spectrometry

scientific article

Partial unfolding of diverse SH3 domains on a wide timescale

scientific article

Pepsin immobilized on high-strength hybrid particles for continuous flow online digestion at 10,000 psi.

scientific article

Phosphorylation and structure-based functional studies reveal a positive and a negative role for the activation loop of the c-Abl tyrosine kinase.

scientific article published on December 2001

Post-translational modifications differentially affect IgG1 conformation and receptor binding.

scientific article

Probing Protein Interactions Using Hydrogen-Deuterium Exchange Mass Spectrometry

Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii

scientific article published on November 2007

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

scientific article published on 27 June 2019

Regulation of c-Fes tyrosine kinase activity by coiled-coil and SH2 domains: analysis with Saccharomyces cerevisiae

scientific article published in April 2003

Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding.

scientific article published on 16 December 2017

Remodeling of the Binding Site of Nucleoside Diphosphate Kinase Revealed by X-ray Structure and H/D Exchange

scientific article published on 04 March 2019

Replication in bioanalytical studies with HDX MS: aim as high as possible

scientific article published on January 2015

Resolving chaperone-assisted protein folding on the ribosome at the peptide level

scientific article published on 10 July 2024

Selective USP7 inhibition elicits cancer cell killing through a p53-dependent mechanism

scientific article published on 24 March 2020

Semi-automated data processing of hydrogen exchange mass spectra using HX-Express

scientific article

Site-Dependent Cysteine Lipidation Potentiates the Activation of Proapoptotic BAX

scientific article published on 01 March 2020

Specialized Methods

book chapter published in 2007

Src family kinases phosphorylate the Bcr-Abl SH3-SH2 region and modulate Bcr-Abl transforming activity

scientific article

Steric gate residues of Y-family DNA polymerases DinB and pol kappa are crucial for dNTP-induced conformational change

scientific article

Structural Basis for Lipid Binding and Function by an Evolutionarily Conserved Protein, Serum Amyloid A

scientific article published on 06 February 2020

Structural Dynamics in Ras and Related Proteins upon Nucleotide Switching

scientific article published on 14 October 2016

Structural Stability and Local Dynamics in Disease-Causing Mutants of Human Apolipoprotein A-I: What Makes the Protein Amyloidogenic?

scientific article published on 9 November 2015

Structural analysis of lecithin:cholesterol acyltransferase bound to high density lipoprotein particles

scientific article published on 15 January 2020

Structural basis of the atypical activation mechanism of KRASV14I

scientific article published on 24 July 2019

Structural stability and local dynamics in disease-causing mutants of human apolipoprotein a-I: what makes the protein amyloidogenic?

scientific article published on 31 December 2016

Structure and dynamic regulation of Abl kinases

scientific article published on 11 January 2013

Structure of the Cdc48 ATPase with its ubiquitin-binding cofactor Ufd1-Npl4

scientific article published on 02 July 2018

Structure of the helicase core of Werner helicase, a key target in microsatellite instability cancers

scientific article published on 16 November 2020

Structure, function, and inhibitor targeting of HIV-1 Nef-effector kinase complexes

scientific article published on 29 August 2020

Structure-Guided Development of a Potent and Selective Non-covalent Active-Site Inhibitor of USP7.

scientific article

Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding

scientific article

Subtle Dynamic Changes Accompany Hck Activation by HIV-1 Nef and are Reversed by an Antiretroviral Kinase Inhibitor

scientific article published on 6 October 2015

Sulfonyl fluoride inhibitors of fatty acid amide hydrolase

scientific article

Tag and Capture Flow Hydrogen Exchange Mass Spectrometry with a Fluorous-Immobilized Probe

scientific article published on 29 May 2015

Targeting Bcr–Abl by combining allosteric with ATP-binding-site inhibitors

scientific article

Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer

scientific article published on 17 August 2020

The Abl SH2-kinase linker naturally adopts a conformation competent for SH3 domain binding

scientific article

The Src family kinase Fgr is a transforming oncoprotein that functions independently of SH3-SH2 domain regulation

scientific article published on 23 October 2018

The heme regulatory motifs of heme oxygenase-2 contribute to the transfer of heme to the catalytic site for degradation

scientific article published on 09 March 2020

The influence of adnectin binding on the extracellular domain of epidermal growth factor receptor

scientific article

The natural product cucurbitacin E inhibits depolymerization of actin filaments

scientific article published on 9 July 2012

The utility of hydrogen/deuterium exchange mass spectrometry in biopharmaceutical comparability studies

scientific article

Therapeutic targeting of oncogenic K-Ras by a covalent catalytic site inhibitor

scientific article published on 20 November 2013

Tissue-Specific Oncogenic Activity of KRASA146T

scientific article published on 05 April 2019

Tuning a High Transmission Ion Guide to Prevent Gas-Phase Proton Exchange During H/D Exchange MS Analysis

scientific article published on 26 January 2016

Tyrosine phosphorylation in the SH3 domain disrupts negative regulatory interactions within the c-Abl kinase core

scientific article

Ultra performance liquid chromatography (UPLC) further improves hydrogen/deuterium exchange mass spectrometry

article

Using hydrogen/deuterium exchange mass spectrometry to study conformational changes in granulocyte colony stimulating factor upon PEGylation

scientific article published on 7 January 2012

Using stable-isotope-labeled proteins for hydrogen exchange studies in complex mixtures

scientific article published in April 2002

Utilizing Microchip Capillary Electrophoresis Electrospray Ionization for Hydrogen Exchange Mass Spectrometry

scientific article published on 2 June 2015

What mass spectrometry can reveal about protein function

scientific article published on 20 February 2004

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