List of works - Nico Callewaert

A Glycomics-Based Test Predicts the Development of Hepatocellular Carcinoma in Cirrhosis.

scientific article published on 16 December 2016

A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes

scientific article

A guide to Mycobacterium mutagenesis

scientific article published on 26 August 2019

A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes

scientific article published on 25 October 2011

A scalable low-cost cGMP process for clinical grade production of the HIV inhibitor 5P12-RANTES in Pichia pastoris

scientific article

A simple colorimetric assay for measuring fructosamine 3 kinase activity

scientific article published on 9 July 2016

Abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to Botrytis cinerea

scientific article published on 13 August 2010

Agalactosyl Immunoglobulins are a Useful Marker for Clinical Progression and Successful Therapy of Collagen-induced Arthritis

Alteration of protein glycosylation in liver diseases

scientific article published on 27 December 2008

Alterations of serum protein N-glycosylation in two mouse models of chronic liver disease are hepatocyte and not B cell driven

scientific article

An endoplasmic reticulum-engineered yeast platform for overproduction of triterpenoids

scientific article published on 16 February 2017

An essential oligomannosidic glycan chain in the catalytic domain of autotaxin, a secreted lysophospholipase-D

scientific journal article

Analysis of gamma-globulin mobility on routine clinical CE equipment: exploring its molecular basis and potential clinical utility

scientific article published in August 2009

Angiogenesis enhances factor IX delivery and persistence from retrievable human bioengineered muscle implants

scientific article published on 5 June 2006

Azoospermia: Virtual Reality or Possible to Quantify?

scientific article published on 05 April 2006

Biologically active, magnICON®-expressed EPO-Fc from stably transformed Nicotiana benthamiana plants presenting tetra-antennary N-glycan structures

scientific article published on 16 March 2012

CE analysis of γ-globulin mobility and potential clinical utility.

scientific article published in January 2013

Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer

scientific article

Characterization of a type D1A EUL-related lectin from rice expressed in Pichia pastoris

scientific article published in April 2014

Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing

scientific article published on 11 May 2015

Clinical laboratory testing in human medicine based on the detection of glycoconjugates

scientific article published on June 2007

Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris.

scientific article published on 30 January 2015

Consequences of Soluble ICAM-1 N-Glycan Alterations on Receptor Binding and Signaling Kinetics in Mouse Astrocytes

Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II.

scholarly article

Definition of the bacterial N-glycosylation site consensus sequence

scientific article

Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered Mycobacterium bovis Bacillus Calmette-Guérin Mutant Resource

scientific article published on 11 August 2020

Diagnostic value of the hemopexin N-glycan profile in hepatocellular carcinoma patients

scientific article

Disruption of the SapM locus in Mycobacterium bovis BCG improves its protective efficacy as a vaccine against M. tuberculosis

scientific article

Efficacy of a trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease.

scientific article published on 25 January 2015

Elucidation of the molecular mechanisms of two nanobodies that inhibit thrombin-activatable fibrinolysis inhibitor activation and activated thrombin-activatable fibrinolysis inhibitor activity

scientific article

Endoglycosidase S enables a highly simplified clinical chemistry assay procedure for direct assessment of serum IgG undergalactosylation in chronic inflammatory disease

Engineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man3GlcNAc2 N-glycan core

scientific article

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

scientific article published in January 2009

Engineering of glycosylation in yeast and other fungi: current state and perspectives

scientific article published on 29 June 2010

Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology

scientific article published in January 2015

Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man₈GlcNAc₂ and Man₅GlcNAc₂.

scientific article published on May 2012

Engineering yeast for producing human glycoproteins: where are we now?

scientific article published on January 2015

Enhanced membrane protein expression by engineering increased intracellular membrane production

scientific article published on 9 December 2013

Enhanced membrane protein expression by engineering increased intracellular membrane production

Expression analysis of the nucleocytoplasmic lectin 'Orysata' from rice in Pichia pastoris

scientific article

Expression and glyco-engineering of alpha-galactosidase A in Pichia pastoris for the treatment of Fabry disease

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

scientific article

Fishing for lectins from diverse sequence libraries by yeast surface display - an exploratory study

scientific article

Genome dynamics of the human embryonic kidney 293 (HEK293) lineage in response to cell biology manipulations

scholarly article by Morgane Boone published in July 2014

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

scientific article

Genome sequence of the recombinant protein production host Pichia pastoris

scientific article

GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins.

scientific article published on 20 April 2014

GlycoDelete technology: shortcutting mammalian cell N-glycosylation

GlycoFibroTest is a highly performant liver fibrosis biomarker derived from DNA sequencer-based serum protein glycomics

scientific article published on 29 January 2009

Glycome Patterns of Perfusate in Livers Before Transplantation Associate With Primary Non-function

scientific article published on 5 January 2018

Glycome mapping on DNA sequencing equipment

scientific article published in January 2006

Glycome profiling using modern glycomics technology: technical aspects and applications

scientific article published on February 2010

Hierarchical and Redundant Roles of Activating FcγRs in Protection against Influenza Disease by M2e-Specific IgG1 and IgG2a Antibodies

scientific article published on 11 January 2017

High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems

scientific article published in January 2013

High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems: toward clinical implementation of GlycoHepatoTest

scientific article published on September 2010

High-throughput quantitative analysis of plant N-glycan using a DNA sequencer

article

Human T cell glycosylation and implications on immune therapy for cancer

scientific article published on 18 March 2020

Hybrid N-glycans on the host protective activation-associated secreted proteins of Ostertagia ostertagi and their importance in immunogenicity

scientific article published on 21 May 2008

Impact of elevation of total bilirubin level and etiology of the liver disease on serum N-glycosylation patterns in mice and humans

scientific article published on 7 January 2010

Improved sample preparation for CE-LIF analysis of plant N-glycans

scientific article published on 21 November 2011

In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris

scientific article

Increased fucosylation and reduced branching of serum glycoprotein N-glycans in all known subtypes of congenital disorder of glycosylation I.

scientific article published on 22 January 2003

Influenza-binding sialylated polymer coated gold nanoparticles prepared via RAFT polymerization and reductive amination

scientific article published on 29 January 2016

Introduction of tri-antennary N-glycans in Arabidopsis thaliana plants

scientific article published on 10 October 2011

Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

scientific article

Long-Lasting Cross-Protection Against Influenza A by Neuraminidase and M2e-based immunization strategies

scientific article

Lyso-Phosphatidylcholine Is a Signal in the Arbuscular Mycorrhizal Symbiosis

scientific article published in Science

Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit

scientific article published on 05 November 2021

Mechanical strain determines the site-specific localization of inflammation and tissue damage in arthritis

scientific article published in Nature Communications

Membrane protein expression and analysis in yeast

scientific article published on 20 March 2015

Minimally invasive classification of paediatric solid tumours using reduced representation bisulphite sequencing of cell-free DNA: a proof-of-principle study

scientific article published on 14 July 2020

Modification of the N -Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids

Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids

scientific article published in January 2007

Modular Integrated Secretory System Engineering in Pichia pastoris To Enhance G-Protein Coupled Receptor Expression

scientific article

Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase

scientific article published on February 2000

Multiple phenotypes in phosphoglucomutase 1 deficiency

scientific article

N-Glycan Engineering in Yeasts and Fungi

article published in 2005

N-glycan based biomarker distinguishing non-alcoholic steatohepatitis from steatosis independently of fibrosis

scientific article published on 25 November 2011

N-glycan structures and N-glycosylation sites of mouse soluble intercellular adhesion molecule-1 revealed by MALDI-TOF and FTICR mass spectrometry

scientific article

N-glycomic changes in hepatocellular carcinoma patients with liver cirrhosis induced by hepatitis B virus

scientific article

N-linked glycosylation of folded proteins by the bacterial oligosaccharyltransferase.

scientific article

Necrostatin-1 analogues: critical issues on the specificity, activity and in vivo use in experimental disease models

scientific article published on 29 November 2012

Necrostatin-1 blocks both RIPK1 and IDO: consequences for the study of cell death in experimental disease models

scientific article published on 30 November 2012

Non-pathogenic trypanosomatid protozoa as a platform for protein research and production

scientific article published on 01 July 2002

Noninvasive diagnosis of liver cirrhosis using DNA sequencer-based total serum protein glycomics

scientific article published in April 2004

O Mannosylation of alpha-dystroglycan is essential for lymphocytic choriomeningitis virus receptor function

scientific article

Open access to sequence: browsing the Pichia pastoris genome

scientific article

P-selectin mediates metastatic progression through binding to sulfatides on tumor cells

scientific article

Pichia Surface Display: A Tool for Screening Single Domain Antibodies ⬇️

scientific article published on January 1, 2012

Pichia pastoris GlycoDelete: the way out when N-glycans are a burden

Pichia surface display: display of proteins on the surface of glycoengineered Pichia pastoris strains

scientific article

Production of complex multiantennary N-glycans in Nicotiana benthamiana plants.

scientific article

Production, purification and crystallization of a trans-sialidase from Trypanosoma vivax

scientific article published on 21 April 2015

Protein Glycosylation as a Diagnostic and Prognostic Marker of Chronic Inflammatory Gastrointestinal and Liver Diseases

scientific article published on 15 October 2019

Reference genome and comparative genome analysis for the WHO reference strain for Mycobacterium bovis BCG Danish, the present tuberculosis vaccine

scientific article published on 08 July 2019

Release of urinary extracellular vesicles in prostate cancer is associated with altered urinary N-glycosylation profile

scientific article

Reversible changes in serum immunoglobulin galactosylation during the immune response and treatment of inflammatory autoimmune arthritis

scientific article published on 4 September 2008

Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity

scientific article published on 01 October 2021

STAT2 signaling restricts viral dissemination but drives severe pneumonia in SARS-CoV-2 infected hamsters

scientific article published on 17 November 2020

Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies

scientific article published on 29 April 2020

Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies

scientific article published on 01 June 2020

Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies

scientific article (preprint)

Structure and Assembly Mechanism of the Signaling Complex Mediated by Human CSF-1

scientific article

Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line

scientific article (publication date: 15 October 2002)

Structure of Ostertagia ostertagi ASP-1: insights into disulfide-mediated cyclization and dimerization

scientific article

Structure of the Gh92 Family Glycosyl Hydrolase Ccman5 in complex with deoxymannojirimycin

Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems

scholarly article

Synthesis of Isofagomine and a New C6 Pyrrolidine Azasugar with Potential Biological Activity

Synthetic biology for production of natural and new-to-nature terpenoids in photosynthetic organisms

scientific article published on 12 February 2016

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

scientific article

Total serum protein N-glycome profiling on a capillary electrophoresis-microfluidics platform

scientific article published in October 2004

Two distinct but interchangeable mechanisms for flipping of lipid-linked oligosaccharides

scientific journal article

Ultrasensitive profiling and sequencing of N-linked oligosaccharides using standard DNA-sequencing equipment.

scientific article

Urinary prostate protein glycosylation profiling as a diagnostic biomarker for prostate cancer

scientific article published on 30 October 2014

Use of a meltable polyacrylamide matrix for sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a procedure for N-glycan analysis on picomole amounts of glycoproteins

scientific article published in April 2002

Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds

scientific article

Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model

scientific article published on 01 April 2019

[N-glycomic changes in hepatocellular carcinoma patients with liver cirrhosis induced by hepatitis B virus]

scientific article published on 01 January 2008

List of works by Nico Callewaert

A Glycomics-Based Test Predicts the Development of Hepatocellular Carcinoma in Cirrhosis.

A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes

A guide to Mycobacterium mutagenesis

A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes

A scalable low-cost cGMP process for clinical grade production of the HIV inhibitor 5P12-RANTES in Pichia pastoris

A simple colorimetric assay for measuring fructosamine 3 kinase activity

Abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to Botrytis cinerea

Agalactosyl Immunoglobulins are a Useful Marker for Clinical Progression and Successful Therapy of Collagen-induced Arthritis

Alteration of protein glycosylation in liver diseases

Alterations of serum protein N-glycosylation in two mouse models of chronic liver disease are hepatocyte and not B cell driven

An endoplasmic reticulum-engineered yeast platform for overproduction of triterpenoids

An essential oligomannosidic glycan chain in the catalytic domain of autotaxin, a secreted lysophospholipase-D

Analysis of gamma-globulin mobility on routine clinical CE equipment: exploring its molecular basis and potential clinical utility

Angiogenesis enhances factor IX delivery and persistence from retrievable human bioengineered muscle implants

Azoospermia: Virtual Reality or Possible to Quantify?

Biologically active, magnICON®-expressed EPO-Fc from stably transformed Nicotiana benthamiana plants presenting tetra-antennary N-glycan structures

CE analysis of γ-globulin mobility and potential clinical utility.

Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer

Characterization of a type D1A EUL-related lectin from rice expressed in Pichia pastoris

Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing

Clinical laboratory testing in human medicine based on the detection of glycoconjugates

Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris.

Consequences of Soluble ICAM-1 N-Glycan Alterations on Receptor Binding and Signaling Kinetics in Mouse Astrocytes

Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II.

Definition of the bacterial N-glycosylation site consensus sequence

Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered Mycobacterium bovis Bacillus Calmette-Guérin Mutant Resource

Diagnostic value of the hemopexin N-glycan profile in hepatocellular carcinoma patients

Disruption of the SapM locus in Mycobacterium bovis BCG improves its protective efficacy as a vaccine against M. tuberculosis

Efficacy of a trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease.

Elucidation of the molecular mechanisms of two nanobodies that inhibit thrombin-activatable fibrinolysis inhibitor activation and activated thrombin-activatable fibrinolysis inhibitor activity

Endoglycosidase S enables a highly simplified clinical chemistry assay procedure for direct assessment of serum IgG undergalactosylation in chronic inflammatory disease

Engineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man3GlcNAc2 N-glycan core

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

Engineering of glycosylation in yeast and other fungi: current state and perspectives

Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology

Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man₈GlcNAc₂ and Man₅GlcNAc₂.

Engineering yeast for producing human glycoproteins: where are we now?

Enhanced membrane protein expression by engineering increased intracellular membrane production

Enhanced membrane protein expression by engineering increased intracellular membrane production

Expression analysis of the nucleocytoplasmic lectin 'Orysata' from rice in Pichia pastoris

Expression and glyco-engineering of alpha-galactosidase A in Pichia pastoris for the treatment of Fabry disease

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Fishing for lectins from diverse sequence libraries by yeast surface display - an exploratory study

Genome dynamics of the human embryonic kidney 293 (HEK293) lineage in response to cell biology manipulations

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

Genome sequence of the recombinant protein production host Pichia pastoris

GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins.

GlycoDelete technology: shortcutting mammalian cell N-glycosylation

GlycoFibroTest is a highly performant liver fibrosis biomarker derived from DNA sequencer-based serum protein glycomics

Glycome Patterns of Perfusate in Livers Before Transplantation Associate With Primary Non-function

Glycome mapping on DNA sequencing equipment

Glycome profiling using modern glycomics technology: technical aspects and applications

Hierarchical and Redundant Roles of Activating FcγRs in Protection against Influenza Disease by M2e-Specific IgG1 and IgG2a Antibodies

High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems

High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems: toward clinical implementation of GlycoHepatoTest

High-throughput quantitative analysis of plant N-glycan using a DNA sequencer

Human T cell glycosylation and implications on immune therapy for cancer

Hybrid N-glycans on the host protective activation-associated secreted proteins of Ostertagia ostertagi and their importance in immunogenicity

Impact of elevation of total bilirubin level and etiology of the liver disease on serum N-glycosylation patterns in mice and humans

Improved sample preparation for CE-LIF analysis of plant N-glycans

In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris

Increased fucosylation and reduced branching of serum glycoprotein N-glycans in all known subtypes of congenital disorder of glycosylation I.

Influenza-binding sialylated polymer coated gold nanoparticles prepared via RAFT polymerization and reductive amination

Introduction of tri-antennary N-glycans in Arabidopsis thaliana plants

Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

Long-Lasting Cross-Protection Against Influenza A by Neuraminidase and M2e-based immunization strategies

Lyso-Phosphatidylcholine Is a Signal in the Arbuscular Mycorrhizal Symbiosis

Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit

Mechanical strain determines the site-specific localization of inflammation and tissue damage in arthritis

Membrane protein expression and analysis in yeast

Minimally invasive classification of paediatric solid tumours using reduced representation bisulphite sequencing of cell-free DNA: a proof-of-principle study

Modification of the N -Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids

Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids

Modular Integrated Secretory System Engineering in Pichia pastoris To Enhance G-Protein Coupled Receptor Expression

Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase

Multiple phenotypes in phosphoglucomutase 1 deficiency

N-Glycan Engineering in Yeasts and Fungi

N-glycan based biomarker distinguishing non-alcoholic steatohepatitis from steatosis independently of fibrosis

N-glycan structures and N-glycosylation sites of mouse soluble intercellular adhesion molecule-1 revealed by MALDI-TOF and FTICR mass spectrometry