List of works - David Piedrafita

A grand scale challenge: vaccine discovery for helminth control in definitive hosts ⬇️

scientific article published on 01 May 2012

A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites

scientific article

A novel ex vivo immunoproteomic approach characterising Fasciola hepatica tegumental antigens identified using immune antibody from resistant sheep.

scientific article published on 25 April 2017

Antibody-dependent cell-mediated cytotoxicity to newly excysted juvenile Fasciola hepatica in vitro is mediated by reactive nitrogen intermediates

scientific article published on 01 September 2001

Cloning and expression of the major secreted cathepsin B-like protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection.

scientific article

Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

scientific article published on 28 July 2015

Comparative experimental Haemonchus contortus infection of two sheep breeds native to the Canary Islands.

scientific article published on 4 March 2008

Current status for gastrointestinal nematode diagnosis in small ruminants: where are we and where are we going?

scientific article

DNA vaccines in sheep: CTLA-4 mediated targeting and CpG motifs enhance immunogenicity in a DNA prime/protein boost strategy

scientific article published on 06 September 2005

Development and application of a fecal antigen diagnostic sandwich ELISA for estimating prevalence of Fasciola gigantica in cattle in central Java, Indonesia

scientific article

Discovery of novel Schistosoma japonicum antigens using a targeted protein microarray approach.

scientific article

Dual oxidase 2 and glutathione peroxidase gene expression are elevated in hyperimmunized sheep challenged with Haemonchus contortus.

scientific article published on 2 March 2011

Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge

scientific article published on 11 September 2015

Effective pulmonary delivery of an aerosolized plasmid DNA vaccine via surface acoustic wave nebulization

scientific article

Effects of nitric oxide on the induction and differentiation of Th1 cells

scientific article published on 01 August 1999

Evaluation of a dry powder delivery system for laninamivir in a ferret model of influenza infection

scientific article published on 26 May 2015

Evaluation of the immune responses induced by four targeted DNA vaccines encoding the juvenile liver fluke antigen, cathepsin B in a mouse model

scientific article

Exploiting natural immunity to helminth parasites for the development of veterinary vaccines

scientific article

Fasciola hepatica and Fasciola gigantica: cloning and characterisation of 70 kDa heat-shock proteins reveals variation in HSP70 gene expression between parasite species recovered from sheep.

scientific article

Fecundity in adult Haemonchus contortus parasites is correlated with abomasal tissue eosinophils and γδ T cells in resistant Canaria Hair Breed sheep ⬇️

scientific article published on January 18, 2011

Field vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus, confers significant protection against an experimental challenge infection

scientific article published on 27 October 2012

Galectin secretion and binding to adult Fasciola hepatica during chronic liver fluke infection of sheep

scientific article published on 21 December 2011

Galectin-11: A novel host mediator targeting specific stages of the gastrointestinal nematode parasite, Haemonchus contortus.

scientific article published on 24 July 2015

Generation of a Novel Bacteriophage Library Displaying scFv Antibody Fragments from the Natural Buffalo Host to Identify Antigens from Adult Schistosoma japonicum for Diagnostic Development

scientific article

Host responses during experimental infection with Fasciola gigantica or Fasciola hepatica in Merino sheep I. Comparative immunological and plasma biochemical changes during early infection

scientific article published on 27 October 2006

Identification of two allelic forms of ovine CD4 exhibiting a Ser183/Pro183 polymorphism in the coding sequence of domain 3.

scientific article

Immune cell kinetics in the ovine abomasal mucosa following hyperimmunization and challenge with Haemonchus contortus

scientific article

Immune mechanisms of resistance to gastrointestinal nematode infections in sheep.

scientific article published on August 2010

Immunity to Haemonchus contortus and Vaccine Development.

scientific article published on 20 April 2016

Increased production through parasite control: can ancient breeds of sheep teach us new lessons?

scientific article

Innate and adaptive resistance of Indonesian Thin Tail sheep to liver fluke: a comparative analysis of Fasciola gigantica and Fasciola hepatica infection.

scientific article published on 26 January 2011

Juvenile Fasciola hepatica are resistant to killing in vitro by free radicals compared with larvae of Schistosoma mansoni.

scientific article

Liver fluke vaccines in ruminants: strategies, progress and future opportunities.

scientific article published on 06 September 2014

Local immune responses of the Chinese water buffalo, Bubalus bubalis, against Schistosoma japonicum larvae: crucial insights for vaccine design

scientific article

Low cost whole-organism screening of compounds for anthelmintic activity.

scientific article published on 5 March 2015

Modulation of Haemonchus contortus infection by depletion of γδ+ T cells in parasite resistant Canaria Hair Breed sheep

scientific article published on 21 February 2017

Molecular cloning and characterisation of ovine dual oxidase 2.

scientific article

Peritoneal lavage cells of Indonesian thin-tail sheep mediate antibody-dependent superoxide radical cytotoxicity in vitro against newly excysted juvenile Fasciola gigantica but not juvenile Fasciola hepatica

scientific article published on 8 January 2007

Potential role for mucosal IgA in modulating Haemonchus contortus adult worm infection in sheep.

scientific article

Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus.

scientific article published on 19 March 2018

Resistance to liver fluke infection in the natural sheep host is correlated with a type-1 cytokine response.

scientific article

Selection of multi-drug resistant influenza A and B viruses under zanamivir pressure and their replication fitness in ferrets.

scientific article published on 14 February 2017

Selective Expression of a Stable Cell Surface Molecule on Type 2 but Not Type 1 Helper T Cells ⬇️

scientific article published on March 2, 1998

Specific humoral response of hosts with variable schistosomiasis susceptibility.

scientific article

Teladorsagia circumcincta ⬇️

scientific article published in 2019 in WikiJournal of Science

The developing schistosome worms elicit distinct immune responses in different tissue regions.

scientific article

The effect of different adjuvants on immune parameters and protection following vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus

scientific article

The effect of plant tissue and vaccine formulation on the oral immunogenicity of a model plant-made antigen in sheep

scientific article

The in vitro anthelmintic effects of plumbagin on newly excysted and 4-weeks-old juvenile parasites of Fasciola gigantica.

scientific article

The kinetics of local cytokine and galectin expression after challenge infection with the gastrointestinal nematode, Haemonchus contortus.

scientific article published on 21 December 2010

The relationship between the rapid rejection of Haemonchus contortus larvae with cells and mediators in abomasal tissues in immune sheep.

scientific article published on 18 June 2009

Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum

scientific article published on 24 June 2015

Vaccination against fasciolosis by a multivalent vaccine of stage-specific antigens

scientific article

List of works by David Piedrafita

A grand scale challenge: vaccine discovery for helminth control in definitive hosts ⬇️

A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites

A novel ex vivo immunoproteomic approach characterising Fasciola hepatica tegumental antigens identified using immune antibody from resistant sheep.

Antibody-dependent cell-mediated cytotoxicity to newly excysted juvenile Fasciola hepatica in vitro is mediated by reactive nitrogen intermediates

Cloning and expression of the major secreted cathepsin B-like protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection.

Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

Comparative experimental Haemonchus contortus infection of two sheep breeds native to the Canary Islands.

Current status for gastrointestinal nematode diagnosis in small ruminants: where are we and where are we going?

DNA vaccines in sheep: CTLA-4 mediated targeting and CpG motifs enhance immunogenicity in a DNA prime/protein boost strategy

Development and application of a fecal antigen diagnostic sandwich ELISA for estimating prevalence of Fasciola gigantica in cattle in central Java, Indonesia

Discovery of novel Schistosoma japonicum antigens using a targeted protein microarray approach.

Dual oxidase 2 and glutathione peroxidase gene expression are elevated in hyperimmunized sheep challenged with Haemonchus contortus.

Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge

Effective pulmonary delivery of an aerosolized plasmid DNA vaccine via surface acoustic wave nebulization

Effects of nitric oxide on the induction and differentiation of Th1 cells

Evaluation of a dry powder delivery system for laninamivir in a ferret model of influenza infection

Evaluation of the immune responses induced by four targeted DNA vaccines encoding the juvenile liver fluke antigen, cathepsin B in a mouse model

Exploiting natural immunity to helminth parasites for the development of veterinary vaccines

Fasciola hepatica and Fasciola gigantica: cloning and characterisation of 70 kDa heat-shock proteins reveals variation in HSP70 gene expression between parasite species recovered from sheep.

Fecundity in adult Haemonchus contortus parasites is correlated with abomasal tissue eosinophils and γδ T cells in resistant Canaria Hair Breed sheep ⬇️

Field vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus, confers significant protection against an experimental challenge infection

Galectin secretion and binding to adult Fasciola hepatica during chronic liver fluke infection of sheep

Galectin-11: A novel host mediator targeting specific stages of the gastrointestinal nematode parasite, Haemonchus contortus.

Generation of a Novel Bacteriophage Library Displaying scFv Antibody Fragments from the Natural Buffalo Host to Identify Antigens from Adult Schistosoma japonicum for Diagnostic Development

Host responses during experimental infection with Fasciola gigantica or Fasciola hepatica in Merino sheep I. Comparative immunological and plasma biochemical changes during early infection

Identification of two allelic forms of ovine CD4 exhibiting a Ser183/Pro183 polymorphism in the coding sequence of domain 3.

Immune cell kinetics in the ovine abomasal mucosa following hyperimmunization and challenge with Haemonchus contortus

Immune mechanisms of resistance to gastrointestinal nematode infections in sheep.

Immunity to Haemonchus contortus and Vaccine Development.

Increased production through parasite control: can ancient breeds of sheep teach us new lessons?

Innate and adaptive resistance of Indonesian Thin Tail sheep to liver fluke: a comparative analysis of Fasciola gigantica and Fasciola hepatica infection.

Juvenile Fasciola hepatica are resistant to killing in vitro by free radicals compared with larvae of Schistosoma mansoni.

Liver fluke vaccines in ruminants: strategies, progress and future opportunities.

Local immune responses of the Chinese water buffalo, Bubalus bubalis, against Schistosoma japonicum larvae: crucial insights for vaccine design

Low cost whole-organism screening of compounds for anthelmintic activity.

Modulation of Haemonchus contortus infection by depletion of γδ+ T cells in parasite resistant Canaria Hair Breed sheep

Molecular cloning and characterisation of ovine dual oxidase 2.

Peritoneal lavage cells of Indonesian thin-tail sheep mediate antibody-dependent superoxide radical cytotoxicity in vitro against newly excysted juvenile Fasciola gigantica but not juvenile Fasciola hepatica

Potential role for mucosal IgA in modulating Haemonchus contortus adult worm infection in sheep.

Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus.

Resistance to liver fluke infection in the natural sheep host is correlated with a type-1 cytokine response.

Selection of multi-drug resistant influenza A and B viruses under zanamivir pressure and their replication fitness in ferrets.

Selective Expression of a Stable Cell Surface Molecule on Type 2 but Not Type 1 Helper T Cells ⬇️

Specific humoral response of hosts with variable schistosomiasis susceptibility.

Teladorsagia circumcincta ⬇️

The developing schistosome worms elicit distinct immune responses in different tissue regions.

The effect of different adjuvants on immune parameters and protection following vaccination of sheep with a larval-specific antigen of the gastrointestinal nematode, Haemonchus contortus

The effect of plant tissue and vaccine formulation on the oral immunogenicity of a model plant-made antigen in sheep

The in vitro anthelmintic effects of plumbagin on newly excysted and 4-weeks-old juvenile parasites of Fasciola gigantica.

The kinetics of local cytokine and galectin expression after challenge infection with the gastrointestinal nematode, Haemonchus contortus.

The relationship between the rapid rejection of Haemonchus contortus larvae with cells and mediators in abomasal tissues in immune sheep.

Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum

Vaccination against fasciolosis by a multivalent vaccine of stage-specific antigens