List of works - Joachim Goedhart

A FRET-based biosensor for measuring Gα13 activation in single cells.

scientific article published on 5 March 2018

A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells

scientific article published on 22 January 2016

A Perspective on Studying G-Protein-Coupled Receptor Signaling with Resonance Energy Transfer Biosensors in Living Organisms

scientific article

A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1.

scientific article

A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range

scientific article

An introduction to fluorescence imaging techniques geared towards biosensor applications.

scientific article

Analysis of oligonucleotide annealing by electrophoresis in agarose gels using sodium borate conductive medium

scientific article published on 01 August 2005

Bright monomeric red fluorescent protein with an extended fluorescence lifetime

scientific article published on 17 June 2007

Bypassing GPCRs with chemical dimerizers

scientific article published on 01 September 2011

Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2.

scientific article published on 20 September 2017

Combination of a spinning disc confocal unit with frequency-domain fluorescence lifetime imaging microscopy.

scientific article

Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius

scientific article published in May 2006

Development of FRET biosensors for mammalian and plant systems.

scientific article published on 12 December 2013

Domain analysis of the Nematostella vectensis SNAIL ortholog reveals unique nucleolar localization that depends on the zinc-finger domains

scientific article published on 20 July 2015

Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC.

scientific article

Effect of fixation procedures on the fluorescence lifetimes of Aequorea victoria derived fluorescent proteins

scientific article published on September 2014

F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling

scientific article

Fluorescence resonance energy transfer imaging of PKC signalling in living cells using genetically encoded fluorescent probes.

scientific article published on 28 October 2008

Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry and with high affinity

scientific article published on 14 April 2015

Identical accumulation and immobilization of sulfated and nonsulfated Nod factors in host and nonhost root hair cell walls

scientific article published on 01 October 2003

Identification of guanine nucleotide exchange factors that increase Cdc42 activity in primary human endothelial cells

scientific article published on 30 August 2019

Imaging phosphatidylinositol 4-phosphate dynamics in living plant cells.

scientific article

Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells

scientific article published on 27 February 2007

In vivo characterisation of fluorescent proteins in budding yeast

scientific article published on 19 February 2019

In vivo fluorescence correlation microscopy (FCM) reveals accumulation and immobilization of Nod factors in root hair cell walls

scientific article published on 01 January 2000

Molecular perturbation strategies to examine spatiotemporal features of Rho GEF and Rho GTPase activity in living cells.

scientific article published on 19 May 2017

Multiparameter imaging for the analysis of intracellular signaling.

scientific article

Nod factors integrate spontaneously in biomembranes and transfer rapidly between membranes and to root hairs, but transbilayer flip-flop does not occur

scientific article published on 01 August 1999

Optimization of fluorescent proteins.

scientific article

PKC gamma mutations in spinocerebellar ataxia type 14 affect C1 domain accessibility and kinase activity leading to aberrant MAPK signaling.

scientific article published on 24 June 2008

PLCβ isoforms differ in their subcellular location and their CT-domain dependent interaction with Gαq.

scientific article

Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells[W] ⬇️

scientific article published on September 24, 2003

Plant G protein heterotrimers require dual lipidation motifs of Galpha and Ggamma and do not dissociate upon activation

scientific article published on 01 December 2006

Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization

scientific article

PlotTwist: A web app for plotting and annotating continuous data

scientific article published on 13 January 2020

PlotXpress, a webtool for normalization and visualization of reporter expression data

scientific article published on 08 November 2021

PlotsOfData-A web app for visualizing data together with their summaries

scientific article published on 27 March 2019

Practical and reliable FRET/FLIM pair of fluorescent proteins

scientific article

Publisher Correction: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II

scientific article published on 06 November 2020

Quantitative Single-Cell Analysis of Signaling Pathways Activated Immediately Downstream of Histamine Receptor Subtypes.

scientific article published on 29 June 2016

Quantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor.

scientific article

Quantitative lifetime unmixing of multiexponentially decaying fluorophores using single-frequency fluorescence lifetime imaging microscopy

scientific article

Rapid colorimetric quantification of lipo-chitooligosaccharides from Mesorhizobium loti and Sinorhizobium meliloti.

scientific article

Real-time visualization of heterotrimeric G protein Gq activation in living cells

scientific article

SCA14 mutation V138E leads to partly unfolded PKCγ associated with an exposed C-terminus, altered kinetics, phosphorylation and enhanced insolubilization

scientific article published on 13 November 2013

Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples

scientific article

Signaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular location

scientific article (publication date: 2013)

Single-cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G-protein-coupled receptors

scientific article published on 11 March 2022

Spatiotemporal analysis of RhoA/B/C activation in primary human endothelial cells

scientific article

Stochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair.

scientific article

Structure of a fluorescent protein fromAequorea victoriabearing the obligate-monomer mutation A206K

scientific article

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.

scientific article

Studentsourcing—Aggregating and reusing data from a practical cell biology course

scientific article published in 2024

Tetraspanin microdomains control localized protein kinase C signaling in B cells

scientific article published on 9 May 2017

The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling.

scientific article published on 7 June 2018

The anti-apoptotic activity associated with phosphatidylinositol transfer protein alpha activates the MAPK and Akt/PKB pathway

scientific article

The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II

scientific article published on 30 April 2020

Transfection of cells with DNA encoding a visible fluorescent protein-tagged lipid-binding domain.

scientific article published on July 2010

Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia.

scientific article published on 31 October 2003

VolcaNoseR is a web app for creating, exploring, labeling and sharing volcano plots

scientific article published on 25 November 2020

siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.

scientific article

List of works by Joachim Goedhart

A FRET-based biosensor for measuring Gα13 activation in single cells.

A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells

A Perspective on Studying G-Protein-Coupled Receptor Signaling with Resonance Energy Transfer Biosensors in Living Organisms

A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1.

A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range

An introduction to fluorescence imaging techniques geared towards biosensor applications.

Analysis of oligonucleotide annealing by electrophoresis in agarose gels using sodium borate conductive medium

Bright monomeric red fluorescent protein with an extended fluorescence lifetime

Bypassing GPCRs with chemical dimerizers

Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2.

Combination of a spinning disc confocal unit with frequency-domain fluorescence lifetime imaging microscopy.

Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius

Development of FRET biosensors for mammalian and plant systems.

Domain analysis of the Nematostella vectensis SNAIL ortholog reveals unique nucleolar localization that depends on the zinc-finger domains

Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC.

Effect of fixation procedures on the fluorescence lifetimes of Aequorea victoria derived fluorescent proteins

F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling

Fluorescence resonance energy transfer imaging of PKC signalling in living cells using genetically encoded fluorescent probes.

Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry and with high affinity

Identical accumulation and immobilization of sulfated and nonsulfated Nod factors in host and nonhost root hair cell walls

Identification of guanine nucleotide exchange factors that increase Cdc42 activity in primary human endothelial cells

Imaging phosphatidylinositol 4-phosphate dynamics in living plant cells.

Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells

In vivo characterisation of fluorescent proteins in budding yeast

In vivo fluorescence correlation microscopy (FCM) reveals accumulation and immobilization of Nod factors in root hair cell walls

Molecular perturbation strategies to examine spatiotemporal features of Rho GEF and Rho GTPase activity in living cells.

Multiparameter imaging for the analysis of intracellular signaling.

Nod factors integrate spontaneously in biomembranes and transfer rapidly between membranes and to root hairs, but transbilayer flip-flop does not occur

Optimization of fluorescent proteins.

PKC gamma mutations in spinocerebellar ataxia type 14 affect C1 domain accessibility and kinase activity leading to aberrant MAPK signaling.

PLCβ isoforms differ in their subcellular location and their CT-domain dependent interaction with Gαq.

Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells[W] ⬇️

Plant G protein heterotrimers require dual lipidation motifs of Galpha and Ggamma and do not dissociate upon activation

Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization

PlotTwist: A web app for plotting and annotating continuous data

PlotXpress, a webtool for normalization and visualization of reporter expression data

PlotsOfData-A web app for visualizing data together with their summaries

Practical and reliable FRET/FLIM pair of fluorescent proteins

Publisher Correction: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II

Quantitative Single-Cell Analysis of Signaling Pathways Activated Immediately Downstream of Histamine Receptor Subtypes.

Quantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor.

Quantitative lifetime unmixing of multiexponentially decaying fluorophores using single-frequency fluorescence lifetime imaging microscopy

Rapid colorimetric quantification of lipo-chitooligosaccharides from Mesorhizobium loti and Sinorhizobium meliloti.

Real-time visualization of heterotrimeric G protein Gq activation in living cells

SCA14 mutation V138E leads to partly unfolded PKCγ associated with an exposed C-terminus, altered kinetics, phosphorylation and enhanced insolubilization

Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples

Signaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular location

Single-cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G-protein-coupled receptors

Spatiotemporal analysis of RhoA/B/C activation in primary human endothelial cells

Stochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair.

Structure of a fluorescent protein fromAequorea victoriabearing the obligate-monomer mutation A206K

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.

Studentsourcing—Aggregating and reusing data from a practical cell biology course

Tetraspanin microdomains control localized protein kinase C signaling in B cells

The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling.

The anti-apoptotic activity associated with phosphatidylinositol transfer protein alpha activates the MAPK and Akt/PKB pathway

The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II

Transfection of cells with DNA encoding a visible fluorescent protein-tagged lipid-binding domain.

Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia.

VolcaNoseR is a web app for creating, exploring, labeling and sharing volcano plots

siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.