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List of works by Alexander F. Yakunin

A Bifunctional Polyphosphate Kinase Driving the Regeneration of Nucleoside Triphosphate and Reconstituted Cell-Free Protein Synthesis

scientific article published on 19 December 2019

A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair

scientific article

A family of metal-dependent phosphatases implicated in metabolite damage-control

scientific article

A luminol/iodophenol chemiluminescent detection system for western immunoblots

scientific article published on 01 April 1998

A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats

scientific article

ADP-dependent 6-Phosphofructokinase from Pyrococcus horikoshii OT3: STRUCTURE DETERMINATION AND BIOCHEMICAL CHARACTERIZATION OF PH1645

scientific article

An Inserted α/β Subdomain Shapes the Catalytic Pocket of Lactobacillus johnsonii Cinnamoyl Esterase

scientific article

An updated evolutionary classification of CRISPR-Cas systems

scientific article

Analysis of nuclease activity of Cas1 proteins against complex DNA substrates

scientific article published in January 2015

Application of Time-of-Flight-Secondary Ion Mass Spectrometry for the Detection of Enzyme Activity on Solid Wood Substrates

scientific article published on 26 April 2012

Arabidopsis TH2 Encodes the Orphan Enzyme Thiamin Monophosphate Phosphatase

scientific article published on 27 September 2016

Aspartate dehydrogenase, a novel enzyme identified from structural and functional studies of TM1643

scientific article

Biochemical and Structural Characterization of a Novel Family of Cystathionine β-Synthase Domain Proteins Fused to a Zn Ribbon-Like Domain

scientific article

Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases.

scientific article

Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides

scientific article

Biochemical and Structural Studies of Uncharacterized Protein PA0743 from Pseudomonas aeruginosa Revealed NAD+-dependent L-Serine Dehydrogenase

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Biochemical diversity of carboxyl esterases and lipases from Lake Arreo (Spain): a metagenomic approach

scientific article (publication date: June 2013)

CRISPR RNA binding and DNA target recognition by purified Cascade complexes from Escherichia coli

scientific article

Conditional Epistatic Interaction Maps Reveal Global Functional Rewiring of Genome Integrity Pathways in Escherichia coli

scientific article

Crystal structure of human retinoblastoma binding protein 9

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Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate

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Determinants and Prediction of Esterase Substrate Promiscuity Patterns.

scientific article published on 28 November 2017

Discovery and Functional Characterization of a Yeast Sugar Alcohol Phosphatase

scientific article published on 04 October 2018

Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase

scientific article

Enzyme genomics: Application of general enzymatic screens to discover new enzymes.

scientific article

Evolution and classification of the CRISPR-Cas systems

scientific article (publication date: June 2011)

Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants

scientific article published on 19 December 2019

Experimental validation of in silico model-predicted isocitrate dehydrogenase and phosphomannose isomerase from Dehalococcoides mccartyi

scientific article published on 16 September 2015

Extrachromosomal circular elements targeted by CRISPR-Cas in Dehalococcoides mccartyi are linked to mobilization of reductive dehalogenase genes

scientific article published on 13 August 2018

Functional Diversity of Haloacid Dehalogenase Superfamily Phosphatases from Saccharomyces cerevisiae: BIOCHEMICAL, STRUCTURAL, AND EVOLUTIONARY INSIGHTS.

scientific article published on 12 June 2015

Functional and Structural Characterization of DR_0079 from Deinococcus radiodurans , a Novel Nudix Hydrolase with a Preference for Cytosine (Deoxy)ribonucleoside 5′-Di- and Triphosphates †

scientific article

Functional and Structural Characterization of Four Glutaminases from Escherichia coli and Bacillus subtilis †

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General enzymatic screens identify three new nucleotidases in Escherichia coli. Biochemical characterization of SurE, YfbR, and YjjG.

scientific article published on 15 October 2004

Genetic and biochemical properties of an alkaline phosphatase PhoX family protein found in many bacteria

scientific article published on 24 February 2009

Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica

scientific article

Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family

scientific article published on 21 September 2006

Identification and characterization of carboxyl esterases of gill chamber-associated microbiota in the deep-sea shrimp Rimicaris exoculata by using functional metagenomics

scientific article

In situ proteolysis for protein crystallization and structure determination

scientific article

Integrating Structure, Bioinformatics, and Enzymology to Discover Function: BioH, A NEW CARBOXYLESTERASE FROM ESCHERICHIA COLI

scientific article (publication date: 11 July 2003)

Mapping the Reaction Coordinates of Enzymatic Defluorination

scientific article

Mass spectrometry following mild enzymatic digestion reveals phosphorylation of recombinant proteins in Escherichia coli through mechanisms involving direct nucleotide binding

scientific article

Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT.

scientific article

Metagenomics as a Tool for Enzyme Discovery: Hydrolytic Enzymes from Marine-Related Metagenomes

scientific article published in January 2015

Mining bacterial genomes for novel arylesterase activity

scientific article

Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG

scientific article published on 27 March 2006

Molecular basis of the antimutagenic activity of the house-cleaning inosine triphosphate pyrophosphatase RdgB from Escherichia coli

scientific article

Nuclease activity of the human SAMHD1 protein implicated in the Aicardi-Goutieres syndrome and HIV-1 restriction.

scientific article

Nucleotide degradation and ribose salvage in yeast

scientific article

One-Pot Biocatalytic Transformation of Adipic Acid to 6-Aminocaproic Acid and 1,6-Hexamethylenediamine Using Carboxylic Acid Reductases and Transaminases

scientific article published on 07 January 2020

Polyphosphate-dependent synthesis of ATP and ADP by the family-2 polyphosphate kinases in bacteria

scientific article

Pressure adaptation is linked to thermal adaptation in salt-saturated marine habitats

scientific article

Purification and characterization of pyruvate oxidoreductase from the photosynthetic bacterium Rhodobacter capsulatus

scientific article published on 01 November 1998

Purification and properties of a bacterial-type ferredoxin from the nitrogen-fixing cyanobacterium Anabaena variabilis ATCC29413.

scientific article

Purification and properties of a flavodoxin from the heterocystous cyanobacterium Anabaena sphaerica

scientific article published on 01 August 1993

Rational engineering of 2-deoxyribose-5-phosphate aldolases for the biosynthesis of (R)-1,3-butanediol

scientific article published on 05 December 2019

Refined experimental annotation reveals conserved corrinoid autotrophy in chloroform-respiring Dehalobacter isolates.

scientific article published on 29 November 2016

Regulation of nitrogenase activity in Rhodobacter capsulatus under dark microoxic conditions.

scientific article

Riboneogenesis in Yeast

scientific article

Role of the Streptococcus mutans CRISPR-Cas systems in immunity and cell physiology.

scientific article

SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia.

scientific article

Screening and characterization of novel polyesterases from environmental metagenomes with high hydrolytic activity against synthetic polyesters

article

Sequence- and activity-based screening of microbial genomes for novel dehalogenases

scientific article

Site-directed mutagenesis and stability of the carboxylic acid reductase MAB4714 from Mycobacterium abscessus

scientific article published on 02 August 2019

Structural Insight into the Mechanism of c-di-GMP Hydrolysis by EAL Domain Phosphodiesterases

scientific article

Structural and Biochemical Characterization of the Type II Fructose-1,6-bisphosphatase GlpX from Escherichia coli

scientific article

Structural and biochemical characterization of a novel Mn2+-dependent phosphodiesterase encoded by the yfcE gene.

scientific article

Structural and enzymatic characterization of NanS (YjhS), a 9‐O‐Acetyl N‐acetylneuraminic acid esterase from Escherichia coli O157:H7 ⬇️

scientific article published on May 31, 2011

Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus

scientific article published on 25 April 2015

Structural insight into the mechanism of substrate specificity and catalytic activity of an HD-domain phosphohydrolase: the 5'-deoxyribonucleotidase YfbR from Escherichia coli

scientific article

Structural proteomics: a tool for genome annotation

scientific article published on February 2004

Structure and Activity of the Metal-independent Fructose-1,6-bisphosphatase YK23 from Saccharomyces cerevisiae

scientific article

Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference ⬇️

scientific article published on October 18, 2011

Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium

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Structure and activity of the Pseudomonas aeruginosa hotdog-fold thioesterases PA5202 and PA2801

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Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme

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Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica

scientific article

Structure of PhnP, a phosphodiesterase of the carbon-phosphorus lyase pathway for phosphonate degradation.

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Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum

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Structure-Based Mutational Studies of Substrate Inhibition of Betaine Aldehyde Dehydrogenase BetB from Staphylococcus aureus

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Systematic Genetic Screens Reveal the Dynamic Global Functional Organization of the Bacterial Translation Machinery.

scientific article

The COMBREX project: design, methodology, and initial results

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The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity

scientific article

The Cas6e ribonuclease is not required for interference and adaptation by the E. coli type I-E CRISPR-Cas system.

scientific article published on 26 May 2015

The Chromosomal mazEF Locus of Streptococcus mutans Encodes a Functional Type II Toxin-Antitoxin Addiction System ⬇️

scientific article published on December 23, 2010

The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and phosphatase activities

scientific article

The Legionella pneumophila effector Ceg4 is a phosphotyrosine phosphatase that attenuates activation of eukaryotic MAPK pathways.

scientific article published on 4 January 2018

The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes.

scientific article

The structural basis for methylmalonic aciduria. The crystal structure of archaeal ATP:cobalamin adenosyltransferase

scientific article published on 25 March 2004

Toroidal Structure and DNA Cleavage by the CRISPR-Associated [4Fe-4S] Cluster Containing Cas4 Nuclease SSO0001 from Sulfolobus solfataricus

scientific article

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