List of works - Robert N. Pike

A Molecular Switch Governs the Interaction between the Human Complement Protease C1s and Its Substrate, Complement C4

scientific article

A common fold mediates vertebrate defense and bacterial attack

scientific article

A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites

scientific article

A molecular basis for the association of the HLA-DRB1 locus, citrullination, and rheumatoid arthritis

scientific article

A naturally occurring NAR variable domain binds the Kgp protease from Porphyromonas gingivalis

scientific article

Activation of protease-activated receptor-2 leads to inhibition of osteoclast differentiation

scientific article published on 22 December 2003

Adult and juvenile Fasciola cathepsin L proteases: different enzymes for different roles

scientific article published on 16 December 2010

Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1–P4 specificity reveals an unusual preference

article

Angiotensinogen cleavage by renin: importance of a structurally constrained N-terminus

article

Anti-peptide antibodies to cathepsins B, L and D and type IV collagenase

article

Antithrombin: in control of coagulation

scientific article published on March 2004

Approaches to selective peptidic inhibitors of factor Xa.

scientific article published in July 2006

Assembly of the Type II Secretion System such as Found in Vibrio cholerae Depends on the Novel Pilotin AspS

scientific article

Baboon (Papio ursinus) cathepsin L: purification, characterization and comparison with human and sheep cathepsin L.

scientific article

Bioinformatic approaches for predicting substrates of proteases

scientific article published on February 2011

Cathepsin B proteases of flukes: the key to facilitating parasite control?

scientific article published on 30 June 2010

Characterisation of the activity and stability of single-chain cathepsin L and of proteolytically active cathepsin L/cystatin complexes

scientific article published in July 1992

Characterization of a serine protease homologous to house dust mite group 3 allergens from the scabies mite Sarcoptes scabiei

scientific article published on 7 October 2009

Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymes

scientific article published in October 2003

Cleavage and activation of proteinase-activated receptor-2 on human neutrophils by gingipain-R from Porphyromonas gingivalis

article

Cloning and expression of the major secreted cathepsin B-like protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection.

scientific article

Conformational change in the chromatin remodelling protein MENT

scientific article

Conformational changes in serpins: II. The mechanism of activation of antithrombin by heparin.

scientific article

Control of the coagulation system by serpins. Getting by with a little help from glycosaminoglycans

scientific article

Cooperative effects in the substrate specificity of the complement protease C1s.

scientific article

Corruption of innate immunity by bacterial proteases

scientific article

DNA accelerates the inhibition of human cathepsin V by serpins

scientific article

Determination of the P1', P2' and P3' subsite-specificity of factor Xa.

scientific article

Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate

scientific article

Dissection of Protease-Activated Receptor-1-Dependent and -Independent Responses to Thrombin in Skeletal Myoblasts

article

Effect of O-glycosylation and tyrosinesulfation of leech-derived peptides on binding and inhibitory activity against thrombin

article

Elucidation of the substrate specificity of the C1s protease of the classical complement pathway

scientific article published on 16 September 2005

Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, C1-inhibitor

scientific article

Enzymic, phylogenetic, and structural characterization of the unusual papain-like protease domain of Plasmodium falciparum SERA5.

scientific article published on 17 September 2003

Erratum to “Inhibition of osteoblast apoptosis by thrombin” [Bone 33 (2003) 733–743]

scholarly article published in Bone

Evidence for the activation of PAR-2 by the sperm protease, acrosin: expression of the receptor on oocytes

scientific article published in November 2000

Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases

scientific article

Expression of protease-activated receptor-2 by osteoblasts.

scientific article

Fasciola hepatica and Fasciola gigantica: cloning and characterisation of 70 kDa heat-shock proteins reveals variation in HSP70 gene expression between parasite species recovered from sheep.

scientific article

Fluorinated β²- and β³-Amino Acids: Synthesis and Inhibition of α-Chymotrypsin

Functional responses of bone cells to thrombin

scientific article published on August 2006

GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop

scientific article

High molecular weight gingipains from Porphyromonas gingivalis induce cytokine responses from human macrophage-like cells via a nonproteolytic mechanism

scientific article

Highlight: The Biology of Proteolytic Systems

article

Host andPorphyromonas gingivalis proteinases in periodontitis: A biochemical model of infection and tissue destruction

article

Hurpin is a selective inhibitor of lysosomal cathepsin L and protects keratinocytes from ultraviolet-induced apoptosis

scientific article

Identification of a catalytic exosite for complement component C4 on the serine protease domain of C1s.

scientific article published on August 2012

Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates

scientific article published in December 2003

Inhibition of osteoblast apoptosis by thrombin

scientific article published on October 2003

Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation

scientific article

Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability

scientific article published in September 2003

Investigation of the mechanism of interaction between Mannose-binding lectin-associated serine protease-2 and complement C4.

scientific article

Isolation of cathepsin D using three-phase partitioning in t-butanol/water/ammonium sulfate

scientific article published on 01 July 1989

Mannose-binding lectin serine proteases and associated proteins of the lectin pathway of complement: two genes, five proteins and many functions?

scientific article published on 06 June 2011

Mature cathepsin L is substantially active in the ionic milieu of the extracellular medium.

scientific article published in December 1995

Methods to Measure the Kinetics of Protease Inhibition by Serpins

article

Modulation of the proteolytic activity of the complement protease C1s by polyanions: implications for polyanion-mediated acceleration of interaction between C1s and SERPING1.

scientific article

Molecular characterization of centerin, a germinal centre cell serpin

scientific article

Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide

scientific article published on 19 February 2002

Molecular determinants of the substrate specificity of the complement-initiating protease, C1r.

scientific article

Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4.

scientific article

Novel scabies mite serpins inhibit the three pathways of the human complement system

scientific article

Osteopontin and skeletal muscle myoblasts: association with muscle regeneration and regulation of myoblast function in vitro

scientific article

PROSPER: an integrated feature-based tool for predicting protease substrate cleavage sites

scientific article

Phylogeny of the serpin superfamily: implications of patterns of amino acid conservation for structure and function.

scientific article

Physical characterization of serpin conformations

scientific article

PoPS: a computational tool for modeling and predicting protease specificity

scientific article published in June 2005

Predicting serpin/protease interactions

scientific article

Production and processing of a recombinant Fasciola hepatica cathepsin B-like enzyme (FhcatB1) reveals potential processing mechanisms in the parasite

scientific article

Production of anti-peptide antibodies against trypanopain-Tb from Trypanosoma brucei brucei: effects of antibodies on enzyme activity against Z-Phe-Arg-AMC

article

Protease-activated receptor 2 has pivotal roles in cellular mechanisms involved in experimental periodontitis

scientific article

Protease-activated receptors: a means of converting extracellular proteolysis into intracellular signals.

scientific article

Proteinase-activated receptor-2 (PAR2) and mouse osteoblasts: regulation of cell function and lack of specificity of PAR2-activating peptides

scientific article published on 21 September 2009

Proteinase-activated receptor-2 is required for normal osteoblast and osteoclast differentiation during skeletal growth and repair.

scientific article published on 7 December 2011

Proteolytically active complexes of cathepsin L and a cysteine proteinase inhibitor; purification and demonstration of their formation in vitro

scientific article

Recruitment of Human C1 Esterase Inhibitor Controls Complement Activation on Blood Stage Plasmodium falciparum Merozoites

scientific article published on 8 May 2017

S1 Pocket of a Bacterially Derived Subtilisin-like Protease Underpins Effective Tissue Destruction

scientific article

Scabies mite inactivated serine protease paralogs inhibit the human complement system.

scientific article published in June 2009

Scabies mite inactive serine proteases are potent inhibitors of the human complement lectin pathway

scientific article

Serpins 2005 - fun between the beta-sheets. Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia).

scientific article published in October 2005

Serpins and the Complement System

article

Serpins in the Caenorhabditis elegans genome

scientific article published on 01 July 1999

Serpins: finely balanced conformational traps

scientific article

Structural basis for substrate specificity of Helicobacter pylori M17 aminopeptidase

scientific article

Structural characterization of the mechanism through which human glutamic acid decarboxylase auto-activates

scientific article

Structural mechanisms of inactivation in scabies mite serine protease paralogues

scientific article

Studies on the receptors mediating responses of osteoblasts to thrombin.

scientific article published in January 2005

Subsite cooperativity in protease specificity

scientific article published on May 2009

The C-terminal domains of the gingipain K polyprotein are necessary for assembly of the active enzyme and expression of associated activities

scientific article published in December 2004

The Structural Basis for Complement Inhibition by Gigastasin, a Protease Inhibitor from the Giant Amazon Leech.

scientific article

The Subtilisin-Like Protease AprV2 Is Required for Virulence and Uses a Novel Disulphide-Tethered Exosite to Bind Substrates

scientific article

The X-ray Crystal Structure of Mannose-binding Lectin-associated Serine Proteinase-3 Reveals the Structural Basis for Enzyme Inactivity Associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) Syndrome

scientific article

The X-ray crystal structure of full-length human plasminogen

scientific article

The amino acid sequences, structure comparisons and inhibition kinetics of sheep cathepsin L and sheep stefin B

article

The anticoagulant activation of antithrombin by heparin

scientific article published in December 1997

The effects of exosite occupancy on the substrate specificity of thrombin

scientific article published on 26 July 2009

The elusive role of the potential factor X cation-binding exosite-1 in substrate and inhibitor interactions.

scientific article published on 28 October 2003

The evolution of enzyme specificity in Fasciola spp.

scientific article published in July 2003

The gingipains from Porphyromonas gingivalis do not directly induce osteoclast differentiation in primary mouse bone marrow cultures.

scientific article

The gingipains: scissors and glue of the periodontal pathogen, Porphyromonas gingivalis

scientific article published on May 2009

The initiating proteases of the complement system: controlling the cleavage

scientific article published on 06 August 2007

The lysine-specific gingipain of Porphyromonas gingivalis : importance to pathogenicity and potential strategies for inhibition

scientific article published on January 2011

The molecular switches controlling the interaction between complement proteases of the classical and lectin pathways and their substrates

scientific article published on 07 August 2013

The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins

scientific article

The protease cathepsin L regulates Th17 cell differentiation

scientific article

The role of the lys628 (192) residue of the complement protease, c1s, in interacting with Peptide and protein substrates

scientific article

The x-ray crystal structure of mannose-binding lectin-associated serine proteinase-3 reveals the structural basis for enzyme inactivity associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) syndrome.

scientific article published in September 2013

Thrombin inhibits osteoclast differentiation through a non-proteolytic mechanism.

scientific article published on 23 April 2013

Thrombin-stimulated growth factor and cytokine expression in osteoblasts is mediated by protease-activated receptor-1 and prostanoids

scientific article

Total synthesis of homogeneous variants of hirudin P6: a post-translationally modified anti-thrombotic leech-derived protein.

scientific article

Vector-based RNA interference of cathepsin B1 in Schistosoma mansoni

article

X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation

scientific article

List of works by Robert N. Pike

A Molecular Switch Governs the Interaction between the Human Complement Protease C1s and Its Substrate, Complement C4

A common fold mediates vertebrate defense and bacterial attack

A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites

A molecular basis for the association of the HLA-DRB1 locus, citrullination, and rheumatoid arthritis

A naturally occurring NAR variable domain binds the Kgp protease from Porphyromonas gingivalis

Activation of protease-activated receptor-2 leads to inhibition of osteoclast differentiation

Adult and juvenile Fasciola cathepsin L proteases: different enzymes for different roles

Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1–P4 specificity reveals an unusual preference

Angiotensinogen cleavage by renin: importance of a structurally constrained N-terminus

Anti-peptide antibodies to cathepsins B, L and D and type IV collagenase

Antithrombin: in control of coagulation

Approaches to selective peptidic inhibitors of factor Xa.

Assembly of the Type II Secretion System such as Found in Vibrio cholerae Depends on the Novel Pilotin AspS

Baboon (Papio ursinus) cathepsin L: purification, characterization and comparison with human and sheep cathepsin L.

Bioinformatic approaches for predicting substrates of proteases

Cathepsin B proteases of flukes: the key to facilitating parasite control?

Characterisation of the activity and stability of single-chain cathepsin L and of proteolytically active cathepsin L/cystatin complexes

Characterization of a serine protease homologous to house dust mite group 3 allergens from the scabies mite Sarcoptes scabiei

Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymes

Cleavage and activation of proteinase-activated receptor-2 on human neutrophils by gingipain-R from Porphyromonas gingivalis

Cloning and expression of the major secreted cathepsin B-like protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection.

Conformational change in the chromatin remodelling protein MENT

Conformational changes in serpins: II. The mechanism of activation of antithrombin by heparin.

Control of the coagulation system by serpins. Getting by with a little help from glycosaminoglycans

Cooperative effects in the substrate specificity of the complement protease C1s.

Corruption of innate immunity by bacterial proteases

DNA accelerates the inhibition of human cathepsin V by serpins

Determination of the P1', P2' and P3' subsite-specificity of factor Xa.

Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate

Dissection of Protease-Activated Receptor-1-Dependent and -Independent Responses to Thrombin in Skeletal Myoblasts

Effect of O-glycosylation and tyrosinesulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Elucidation of the substrate specificity of the C1s protease of the classical complement pathway

Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, C1-inhibitor

Enzymic, phylogenetic, and structural characterization of the unusual papain-like protease domain of Plasmodium falciparum SERA5.

Erratum to “Inhibition of osteoblast apoptosis by thrombin” [Bone 33 (2003) 733–743]

Evidence for the activation of PAR-2 by the sperm protease, acrosin: expression of the receptor on oocytes

Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases

Expression of protease-activated receptor-2 by osteoblasts.

Fasciola hepatica and Fasciola gigantica: cloning and characterisation of 70 kDa heat-shock proteins reveals variation in HSP70 gene expression between parasite species recovered from sheep.

Fluorinated β²- and β³-Amino Acids: Synthesis and Inhibition of α-Chymotrypsin

Functional responses of bone cells to thrombin

GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop

High molecular weight gingipains from Porphyromonas gingivalis induce cytokine responses from human macrophage-like cells via a nonproteolytic mechanism

Highlight: The Biology of Proteolytic Systems

Host andPorphyromonas gingivalis proteinases in periodontitis: A biochemical model of infection and tissue destruction

Hurpin is a selective inhibitor of lysosomal cathepsin L and protects keratinocytes from ultraviolet-induced apoptosis

Identification of a catalytic exosite for complement component C4 on the serine protease domain of C1s.

Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates

Inhibition of osteoblast apoptosis by thrombin

Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation

Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability

Investigation of the mechanism of interaction between Mannose-binding lectin-associated serine protease-2 and complement C4.

Isolation of cathepsin D using three-phase partitioning in t-butanol/water/ammonium sulfate

Mannose-binding lectin serine proteases and associated proteins of the lectin pathway of complement: two genes, five proteins and many functions?

Mature cathepsin L is substantially active in the ionic milieu of the extracellular medium.

Methods to Measure the Kinetics of Protease Inhibition by Serpins

Modulation of the proteolytic activity of the complement protease C1s by polyanions: implications for polyanion-mediated acceleration of interaction between C1s and SERPING1.

Molecular characterization of centerin, a germinal centre cell serpin

Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide

Molecular determinants of the substrate specificity of the complement-initiating protease, C1r.

Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4.

Novel scabies mite serpins inhibit the three pathways of the human complement system

Osteopontin and skeletal muscle myoblasts: association with muscle regeneration and regulation of myoblast function in vitro

PROSPER: an integrated feature-based tool for predicting protease substrate cleavage sites

Phylogeny of the serpin superfamily: implications of patterns of amino acid conservation for structure and function.

Physical characterization of serpin conformations

PoPS: a computational tool for modeling and predicting protease specificity

Predicting serpin/protease interactions

Production and processing of a recombinant Fasciola hepatica cathepsin B-like enzyme (FhcatB1) reveals potential processing mechanisms in the parasite

Production of anti-peptide antibodies against trypanopain-Tb from Trypanosoma brucei brucei: effects of antibodies on enzyme activity against Z-Phe-Arg-AMC

Protease-activated receptor 2 has pivotal roles in cellular mechanisms involved in experimental periodontitis

Protease-activated receptors: a means of converting extracellular proteolysis into intracellular signals.

Proteinase-activated receptor-2 (PAR2) and mouse osteoblasts: regulation of cell function and lack of specificity of PAR2-activating peptides

Proteinase-activated receptor-2 is required for normal osteoblast and osteoclast differentiation during skeletal growth and repair.

Proteolytically active complexes of cathepsin L and a cysteine proteinase inhibitor; purification and demonstration of their formation in vitro

Recruitment of Human C1 Esterase Inhibitor Controls Complement Activation on Blood Stage Plasmodium falciparum Merozoites

S1 Pocket of a Bacterially Derived Subtilisin-like Protease Underpins Effective Tissue Destruction

Scabies mite inactivated serine protease paralogs inhibit the human complement system.

Scabies mite inactive serine proteases are potent inhibitors of the human complement lectin pathway