List of works - Karsten Niefind

A Note of Caution on the Role of Halogen Bonds for Protein Kinase/Inhibitor Recognition Suggested by High- And Low-Salt CK2α Complex Structures

scientific article

A subnanomolar fluorescent probe for protein kinase CK2 interaction studies

scientific article

A π-Halogen Bond of Dibenzofuranones with the Gatekeeper Phe113 in Human Protein Kinase CK2 Leads to Potent Tight Binding Inhibitors

scientific article published on 17 February 2018

An EDS1 heterodimer signalling surface enforces timely reprogramming of immunity genes in Arabidopsis

scientific article published in Nature Communications

Atomic resolution structures of R-specific alcohol dehydrogenase from Lactobacillus brevis provide the structural bases of its substrate and cosubstrate specificity.

scientific article published in June 2005

Biochemical characterization of CK2alpha and alpha' paralogues and their derived holoenzymes: evidence for the existence of a heterotrimeric CK2alpha'-holoenzyme forming trimeric complexes.

scientific article

Biochemical characterization of the recombinant human Drosophila homologues Timekeeper and Andante involved in the Drosophila circadian oscillator.

scientific article

Biological properties and structural study of new aminoalkyl derivatives of benzimidazole and benzotriazole, dual inhibitors of CK2 and PIM1 kinases

scientific article published on 22 June 2018

Characterization of CK2 holoenzyme variants with regard to crystallization.

scientific article published in November 2001

Crystal structure of L-2-hydroxyisocaproate dehydrogenase from Lactobacillus confusus at 2.2 A resolution. An example of strong asymmetry between subunits

scientific article

Crystal structure of a ternary complex of D-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei, NAD+ and 2-oxoisocaproate at 1.9 A resolution

scientific article

Crystal structure of cis‐biphenyl‐2,3‐dihydrodiol‐2,3‐dehydrogenase from a PCB degrader at 2.0 Å resolution ⬇️

scientific article published on June 1, 1998

Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor

scientific article published on 26 July 2018

Crystal structure of human protein kinase CK2: insights into basic properties of the CK2 holoenzyme

scientific article

Crystal structure of the catalytic subunit of protein kinase CK2 from Zea mays at 2.1 A resolution.

scientific article

Crystallization and Preliminary Characterization of Crystals of d-2-hydroxyisocaproate Dehydrogenase from Lactobacillus casei

scientific article published on 01 July 1994

Crystallization and preliminary X-ray analysis of a family 51 glycoside hydrolase, the α-L-arabinofuranosidase fromGeobacillus stearothermophilusT-6

article

Crystallization and preliminary X-ray analysis of a hydantoinase from Artrobacter aurescens DSM 3745

scientific article published on 01 November 1996

Crystallization and preliminary characterization of crystals ofR-alcohol dehydrogenase fromLactobacillus brevis

scientific article published on 01 December 2000

Crystallization and preliminary crystallographic analysis of Arabidopsis thaliana EDS1, a key component of plant immunity, in complex with its signalling partner SAG101.

scientific article published on 22 January 2011

Crystallization and preliminary crystallographic analysis of a flavoprotein NADH oxidase from Lactobacillus brevis.

scientific article published on 28 April 2005

Crystallization, preliminary X-ray analysis of a native and selenomethionineD-hydantoinase fromThermussp

scientific article published on 01 September 2000

Design of CK2β-Mimicking Peptides as Tools To Study the CK2α/CK2β Interaction in Cancer Cells

scientific article published on 12 March 2019

Development of a high-throughput screening-compatible assay to identify inhibitors of the CK2α/CK2β interaction.

scientific article published on 16 September 2014

Diacritic Binding of an Indenoindole Inhibitor by CK2α Paralogs Explored by a Reliable Path to Atomic Resolution CK2α' Structures

scientific article published on 19 March 2019

Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to and dissociated from Phytoalexin Deficient4 (PAD4) in Arabidopsis immunity ⬇️

scientific article published on March 17, 2011

Enzymatic activity with an incomplete catalytic spine: insights from a comparative structural analysis of human CK2α and its paralogous isoform CK2α'

scientific article

Evidence for aggregation of protein kinase CK2 in the cell: a novel strategy for studying CK2 holoenzyme interaction by BRET(2).

scientific article published on 23 August 2014

Exploring the intramolecular phosphorylation sites in human Chk2.

scientific article published on 11 September 2008

Expression, purification and crystallization of the catalytic subunit of protein kinase CK2 from Zea mays

scientific article published on 01 January 1998

Improved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer

scientific article published on 24 April 2018

Inclining the purine base binding plane in protein kinase CK2 by exchanging the flanking side-chains generates a preference for ATP as a cosubstrate.

scientific article published on 18 January 2005

Insights from soft X-rays: the chlorine and sulfur sub-structures of a CK2alpha/DRB complex.

scientific article published on 8 July 2008

Interaction between CK2α and CK2β, the Subunits of Protein Kinase CK2: Thermodynamic Contributions of Key Residues on the CK2α Surface ⬇️

scientific article published on December 31, 2010

Mutation of recombinant catalytic subunit alpha of the protein kinase CK2 that affects catalytic efficiency and specificity

scientific article published on 01 April 2000

Primary and secondary interactions between CK2alpha and CK2beta lead to ring-like structures in the crystals of the CK2 holoenzyme.

scientific article

Protein kinase CK2 in health and disease: Protein kinase CK2: from structures to insights.

scientific article published on June 2009

Protein kinase CK2: a catalyst for biology, medicine and structural biochemistry.

scientific article

Purification and characterization of the CK2alpha'-based holoenzyme, an isozyme of CK2alpha: a comparative analysis.

scientific article published on 27 December 2005

Structural Hypervariability of the Two Human Protein Kinase CK2 Catalytic Subunit Paralogs Revealed by Complex Structures with a Flavonol- and a Thieno[2,3-d]pyrimidine-Based Inhibitor

scientific article published on 11 January 2017

Structural basis for signaling by exclusive EDS1 heteromeric complexes with SAG101 or PAD4 in plant innate immunity

scientific article

Structural basis of the constitutive activity of protein kinase CK2.

scientific article published on January 2010

Structure of the human protein kinase CK2 catalytic subunit CK2α' and interaction thermodynamics with the regulatory subunit CK2β

scientific article

The 'regulatory' beta-subunit of protein kinase CK2 negatively influences p53-mediated allosteric effects on Chk2 activation

scientific article published on 01 September 2005

The Structure of an Inverting GH43 β-Xylosidase from Geobacillus stearothermophilus with its Substrate Reveals the Role of the Three Catalytic Residues

scientific article published on 20 March 2006

Unexpected Binding Mode of a Potent Indeno[1,2-b]indole-Type Inhibitor of Protein Kinase CK2 Revealed by Complex Structures with the Catalytic Subunit CK2α and Its Paralog CK2α'.

scientific article published on 13 December 2017

List of works by Karsten Niefind

A Note of Caution on the Role of Halogen Bonds for Protein Kinase/Inhibitor Recognition Suggested by High- And Low-Salt CK2α Complex Structures

A subnanomolar fluorescent probe for protein kinase CK2 interaction studies

A π-Halogen Bond of Dibenzofuranones with the Gatekeeper Phe113 in Human Protein Kinase CK2 Leads to Potent Tight Binding Inhibitors

An EDS1 heterodimer signalling surface enforces timely reprogramming of immunity genes in Arabidopsis

Atomic resolution structures of R-specific alcohol dehydrogenase from Lactobacillus brevis provide the structural bases of its substrate and cosubstrate specificity.

Biochemical characterization of CK2alpha and alpha' paralogues and their derived holoenzymes: evidence for the existence of a heterotrimeric CK2alpha'-holoenzyme forming trimeric complexes.

Biochemical characterization of the recombinant human Drosophila homologues Timekeeper and Andante involved in the Drosophila circadian oscillator.

Biological properties and structural study of new aminoalkyl derivatives of benzimidazole and benzotriazole, dual inhibitors of CK2 and PIM1 kinases

Characterization of CK2 holoenzyme variants with regard to crystallization.

Crystal structure of L-2-hydroxyisocaproate dehydrogenase from Lactobacillus confusus at 2.2 A resolution. An example of strong asymmetry between subunits

Crystal structure of a ternary complex of D-2-hydroxyisocaproate dehydrogenase from Lactobacillus casei, NAD+ and 2-oxoisocaproate at 1.9 A resolution

Crystal structure of cis‐biphenyl‐2,3‐dihydrodiol‐2,3‐dehydrogenase from a PCB degrader at 2.0 Å resolution ⬇️

Crystal structure of highly glycosylated human leukocyte elastase in complex with an S2' site binding inhibitor

Crystal structure of human protein kinase CK2: insights into basic properties of the CK2 holoenzyme

Crystal structure of the catalytic subunit of protein kinase CK2 from Zea mays at 2.1 A resolution.

Crystallization and Preliminary Characterization of Crystals of d-2-hydroxyisocaproate Dehydrogenase from Lactobacillus casei

Crystallization and preliminary X-ray analysis of a family 51 glycoside hydrolase, the α-L-arabinofuranosidase fromGeobacillus stearothermophilusT-6

Crystallization and preliminary X-ray analysis of a hydantoinase from Artrobacter aurescens DSM 3745

Crystallization and preliminary characterization of crystals ofR-alcohol dehydrogenase fromLactobacillus brevis

Crystallization and preliminary crystallographic analysis of Arabidopsis thaliana EDS1, a key component of plant immunity, in complex with its signalling partner SAG101.

Crystallization and preliminary crystallographic analysis of a flavoprotein NADH oxidase from Lactobacillus brevis.

Crystallization, preliminary X-ray analysis of a native and selenomethionineD-hydantoinase fromThermussp

Design of CK2β-Mimicking Peptides as Tools To Study the CK2α/CK2β Interaction in Cancer Cells

Development of a high-throughput screening-compatible assay to identify inhibitors of the CK2α/CK2β interaction.

Diacritic Binding of an Indenoindole Inhibitor by CK2α Paralogs Explored by a Reliable Path to Atomic Resolution CK2α' Structures

Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to and dissociated from Phytoalexin Deficient4 (PAD4) in Arabidopsis immunity ⬇️

Enzymatic activity with an incomplete catalytic spine: insights from a comparative structural analysis of human CK2α and its paralogous isoform CK2α'

Evidence for aggregation of protein kinase CK2 in the cell: a novel strategy for studying CK2 holoenzyme interaction by BRET(2).

Exploring the intramolecular phosphorylation sites in human Chk2.

Expression, purification and crystallization of the catalytic subunit of protein kinase CK2 from Zea mays

Improved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer

Inclining the purine base binding plane in protein kinase CK2 by exchanging the flanking side-chains generates a preference for ATP as a cosubstrate.

Insights from soft X-rays: the chlorine and sulfur sub-structures of a CK2alpha/DRB complex.

Interaction between CK2α and CK2β, the Subunits of Protein Kinase CK2: Thermodynamic Contributions of Key Residues on the CK2α Surface ⬇️

Mutation of recombinant catalytic subunit alpha of the protein kinase CK2 that affects catalytic efficiency and specificity

Primary and secondary interactions between CK2alpha and CK2beta lead to ring-like structures in the crystals of the CK2 holoenzyme.

Protein kinase CK2 in health and disease: Protein kinase CK2: from structures to insights.

Protein kinase CK2: a catalyst for biology, medicine and structural biochemistry.

Purification and characterization of the CK2alpha'-based holoenzyme, an isozyme of CK2alpha: a comparative analysis.

Structural Hypervariability of the Two Human Protein Kinase CK2 Catalytic Subunit Paralogs Revealed by Complex Structures with a Flavonol- and a Thieno[2,3-d]pyrimidine-Based Inhibitor

Structural basis for signaling by exclusive EDS1 heteromeric complexes with SAG101 or PAD4 in plant innate immunity

Structural basis of the constitutive activity of protein kinase CK2.

Structure of the human protein kinase CK2 catalytic subunit CK2α' and interaction thermodynamics with the regulatory subunit CK2β

The 'regulatory' beta-subunit of protein kinase CK2 negatively influences p53-mediated allosteric effects on Chk2 activation

The Structure of an Inverting GH43 β-Xylosidase from Geobacillus stearothermophilus with its Substrate Reveals the Role of the Three Catalytic Residues

Unexpected Binding Mode of a Potent Indeno[1,2-b]indole-Type Inhibitor of Protein Kinase CK2 Revealed by Complex Structures with the Catalytic Subunit CK2α and Its Paralog CK2α'.