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List of works by Christoph Herwig

A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations.

scientific article

A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization

scientific article

A control strategy to investigate the relationship between specific productivity and high-mannose glycoforms in CHO cells ⬇️

scientific article published on 24 February 2016

A dynamic fed batch strategy for a Pichia pastoris mixed feed system to increase process understanding

scientific article published on 21 May 2012

A dynamic method for the investigation of induced state metabolic capacities as a function of temperature

scientific article

A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains.

scientific article published on 27 October 2011

A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis.

scientific article published on 23 December 2014

A novel method for fast and statistically verified morphological characterization of filamentous fungi.

scientific article

A novel method to recover inclusion body protein from recombinant E. coli fed-batch processes based on phage ΦX174-derived lysis protein E.

scientific article published on 20 April 2017

A novel toolbox for E. coli lysis monitoring

scientific article published on 2 September 2016

A rapid method for the differentiation of yeast cells grown under carbon and nitrogen-limited conditions by means of partial least squares discriminant analysis employing infrared micro-spectroscopic data of entire yeast cells

scientific article

A robust feeding strategy to maintain set-point glucose in mammalian fed-batch cultures when input parameters have a large error

scientific article published on 27 January 2017

Advanced Development Strategies for Biopharmaceutical Cell Culture Processes.

scientific article published on 23 July 2015

An Integrated Downstream Process Development Strategy along QbD Principles

scientific article

An automated data-driven DSP development approach for glycoproteins from yeast

scientific article published on 04 August 2017

At-line determination of spore inoculum quality in Penicillium chrysogenum bioprocesses

scientific article published on 28 January 2016

At-line determining spore germination of Penicillium chrysogenum bioprocesses in complex media

scientific article published on 24 August 2016

Automatic feed phase identification in multivariate bioprocess profiles by sequential binary classification.

scientific article

Bioprocess Engineering Aspects of Sustainable Polyhydroxyalkanoate Production in Cyanobacteria

article

Bioprocess development workflow: Transferable physiological knowledge instead of technological correlations

scientific article

Bioprocess monitoring: minimizing sample matrix effects for total protein quantification with bicinchoninic acid assay

scientific article published on 17 June 2016

Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris.

scientific article published on 24 September 2015

Combining light microscopy, dielectric spectroscopy, MALDI intact cell mass spectrometry, FTIR spectromicroscopy and multivariate data mining for morphological and physiological bioprocess characterization of filamentous organisms

scientific article

Combining mechanistic and data-driven approaches to gain process knowledge on the control of the metabolic shift to lactate uptake in a fed-batch CHO process.

scientific article

Comparison of Fiber Optic and Conduit Attenuated Total Reflection (ATR) Fourier Transform Infrared (FT-IR) Setup for In-Line Fermentation Monitoring.

scientific article published on 18 November 2016

Determination of a dynamic feeding strategy for recombinant Pichia pastoris strains

scientific article

Determination of carbohydrates present in Saccharomyces cerevisiae using mid-infrared spectroscopy and partial least squares regression.

scientific article published on 21 August 2013

Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

scientific article published on 8 January 2015

Dynamics in bioprocess development for Pichia pastoris.

scientific article published on 30 October 2014

Effect of post-induction substrate oscillation on recombinant alkaline phosphatase production expressed in Escherichia coli.

scientific article

Effects of temperature shifts and oscillations on recombinant protein production expressed in Escherichia coli

scientific article published on 20 February 2013

Essential prerequisites for successful bioprocess development of biological CH4 production from CO2 and H2.

scientific article published on 11 September 2013

Examining the freezing process of an intermediate bulk containing an industrially relevant protein.

scientific article published on 21 January 2015

Fed-Batch Production of Bacterial Ghosts Using Dielectric Spectroscopy for Dynamic Process Control.

scientific article published on 24 March 2016

Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms

scientific article

Glyco-variant library of the versatile enzyme horseradish peroxidase

scientific article

High throughput inclusion body sizing: Nano particle tracking analysis

scientific article published on 16 March 2017

How to Determine Interdependencies of Glucose and Lactose Uptake Rates for Heterologous Protein Production with E. coli

scientific article published on January 2017

Identification of lipophilic bioproduct portfolio from bioreactor samples of extreme halophilic archaea with HPLC-MS/MS.

scientific article

Impact of Glycerol as Carbon Source onto Specific Sugar and Inducer Uptake Rates and Inclusion Body Productivity in E. coli BL21(DE3).

scientific article published on 21 December 2017

Inclusion Body Bead Size in Controlled by Physiological Feeding

Information Processing: Rate-Based Investigation of Cell Physiological Changes along Design Space Development.

scientific article published in November 2012

Intact cell mass spectrometry as a progress tracking tool for batch and fed-batch fermentation processes

scientific article published on 22 October 2014

Integrated Process Modeling-A Process Validation Life Cycle Companion

scientific article published on 17 October 2017

Investigation of the interactions of critical scale-up parameters (pH, pO2 and pCO2) on CHO batch performance and critical quality attributes

scientific article

Investigation of the physiological response to oxygen limited process conditions of Pichia pastoris Mut(+) strain using a two-compartment scale-down system

scientific article published on 03 May 2013

Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris.

scientific article published on 20 November 2013

Know-how and know-why in biochemical engineering ⬇️

scientific article published on August 1, 2003

Knowledge management in the QbD paradigm: manufacturing of biotech therapeutics.

scientific article published on 13 May 2015

Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains

scientific article published on 23 March 2017

Metabolic Control in Mammalian Fed-Batch Cell Cultures for Reduced Lactic Acid Accumulation and Improved Process Robustness

scientific article published on 11 January 2016

Method for assessing the impact of emission gasses on physiology and productivity in biological methanogenesis

scientific article published on 25 March 2013

Microbials for the production of monoclonal antibodies and antibody fragments.

scientific article published on 31 October 2013

Model-Based Methods in the Biopharmaceutical Process Lifecycle

scientific article published on 22 November 2017

Model-based analysis on the relationship of signal quality to real-time extraction of information in bioprocesses.

scientific article

Morphological analysis of the filamentous fungus Penicillium chrysogenum using flow cytometry-the fast alternative to microscopic image analysis

scientific article published on 14 September 2017

Multi-analyte quantification in bioprocesses by Fourier-transform-infrared spectroscopy by partial least squares regression and multivariate curve resolution

scientific article published on 11 November 2013

Multi-parameter flow cytometry as a process analytical technology (PAT) approach for the assessment of bacterial ghost production.

scientific article published on 31 October 2015

Multivariate Monitoring Workflow for Formulation, Fill and Finish Processes

scientific article published on 03 June 2020

Observability analysis of biochemical process models as a valuable tool for the development of mechanistic soft sensors.

scientific article published on 24 September 2015

Optimal process design space to ensure maximum viability and productivity in Penicillium chrysogenum pellets during fed-batch cultivations through morphological and physiological control

scientific article published on 13 February 2020

Optimization of sample preparation for intact cell mass spectrometry (matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry) of endophytic Xylaria

scientific article published on 01 May 2018

Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

scientific article published on 13 January 2015

Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714.

scientific article

Physiological capacities decline during induced bioprocesses leading to substrate accumulation.

scientific article published on 25 January 2017

Physiological description of multivariate interdependencies between process parameters, morphology and physiology during fed-batch penicillin production

scientific article published on 20 March 2014

Prediction of filamentous process performance attributes by CSL quality assessment using mid-infrared spectroscopy and chemometrics.

scientific article published on 23 November 2017

Predictive Monitoring of Shake Flask Cultures with Online Estimated Growth Models

scientific article published on 08 November 2021

Production and purification of the multifunctional enzyme horseradish peroxidase.

scientific article published on August 2013

Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy

scientific article published on 29 May 2020

Production strategies for active heme-containing peroxidases from E. coli inclusion bodies - a review.

scientific article published on 24 March 2016

Propagation of measurement accuracy to biomass soft-sensor estimation and control quality.

scientific article published on 4 July 2016

Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris.

scientific article published on 14 December 2013

Purification of a recombinant plant peroxidase produced in Pichia pastoris by a simple 2-step strategy

article by Oliver Spadiut et al published 28 September 2012 in Protein Expression and Purification

Quantification of cell lysis during CHO bioprocesses: Impact on cell count, growth kinetics and productivity.

scientific article published on 5 May 2015

Quantitative CPP Evaluation from Risk Assessment Using Integrated Process Modeling

scientific article published on 13 December 2019

Quantitative analysis of the regulation scheme of invertase expression in Saccharomyces cerevisiae.

scientific article published in November 2001

Quantitative comparison of dynamic physiological feeding profiles for recombinant protein production with Pichia pastoris

scientific article published on 10 November 2013

Radial line-scans as representative sampling strategy in dried-droplet laser ablation of liquid samples deposited on pre-cut filter paper disks

scientific article

Reagent-free monitoring of multiple clinically relevant parameters in human blood plasma using a mid-infrared quantum cascade laser based sensor system.

scientific article

Real-time estimation of biomass and specific growth rate in physiologically variable recombinant fed-batch processes.

scientific article

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

scientific article published on 13 February 2012

Risk-based Process Development of Biosimilars as Part of the Quality by Design Paradigm.

scientific article published in November 2013

Role of Knowledge Management in Development and Lifecycle Management of Biopharmaceuticals

scientific article

Science-based bioprocess design for filamentous fungi.

scientific article published on 23 November 2012

Soft Sensor-Based Monitoring and Efficient Control Strategies of Biomass Concentration for Continuous Cultures of Haloferax mediterranei and Their Application to an Industrial Production Chain

scientific article published on 04 December 2019

Soft sensor for monitoring biomass subpopulations in mammalian cell culture processes

scientific article published on 07 August 2017

Stoichiometric and kinetic analysis of extreme halophilic Archaea on various substrates in a corrosion resistant bioreactor.

scientific article published on 29 August 2013

Substrate oscillations boost recombinant protein release from Escherichia coli

scientific article published on 11 October 2013

Switching industrial production processes from complex to defined media: method development and case study using the example of Penicillium chrysogenum.

scientific article published on 22 June 2012

Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli

scientific article published on 20 November 2017

The filamentous fungal pellet-relationship between morphology and productivity.

scientific article published on 22 February 2018

The impact of pH inhomogeneities on CHO cell physiology and fed-batch process performance - two-compartment scale-down modelling and intracellular pH excursion.

scientific article published on 12 January 2017

Toward a Noninvasive, Label-Free Screening Method for Determining Spore Inoculum Quality of Penicillium chrysogenum Using Raman Spectroscopy

scientific article published in January 2017

Tunable recombinant protein expression in E. coli: enabler for continuous processing?

scientific article

Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints

scientific article published on 21 December 2016

Tunable recombinant protein expression with E. coli in a mixed-feed environment.

scientific article published on 14 December 2013

Ultrasound-enhanced attenuated total reflection mid-infrared spectroscopy in-line probe: acquisition of cell spectra in a bioreactor

scientific article

Universal Capacitance Model for Real-Time Biomass in Cell Culture.

scientific article published on 2 September 2015

Workflow for Criticality Assessment Applied in Biopharmaceutical Process Validation Stage 1.

scientific article published on 12 October 2017

Workflow for Target-Oriented Parametrization of an Enhanced Mechanistic Cell Culture Model.

scientific article

Workflow for multi-analyte bioprocess monitoring demonstrated on inline NIR spectroscopy of P. chrysogenum fermentation.

scientific article

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