List of works - Romas J. Kazlauskas

Amplification of screening sensitivity through selective destruction: theory and screening of a library of carbonic anhydrase inhibitors.

scientific article

An inverse substrate orientation for the regioselective acylation of 3',5'-diaminonucleosides catalyzed by Candida antarctica lipase B?

scientific article published in August 2005

Analogs of reaction intermediates identify a unique substrate binding site in Candida rugosa lipase

scientific article

Biocatalysis in ionic liquids - advantages beyond green technology

scientific article

Biocatalysis--becoming more predictable and selective

scientific article published on 01 November 1993

Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system.

scientific article published on 19 October 2012

Biological chemistry: enzymes in focus

scientific article published in August 2005

Biology evolves to fight chemistry

scientific article published on 01 April 2012

Biosynthesis of (-)-5-Hydroxy-equol and 5-Hydroxy-dehydroequol from Soy Isoflavone, Genistein Using Microbial Whole Cell Bioconversion

scientific article

Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases

scientific article published on 6 January 2016

Catalytic promiscuity in biocatalysis: using old enzymes to form new bonds and follow new pathways

scientific article published in November 2004

Chemical biotechnology in progress

scientific article published on 20 August 2011

Cloning, expression and characterization of CYP102D1, a self-sufficient P450 monooxygenase from Streptomyces avermitilis

scientific article

Comparison of Five Protein Engineering Strategies for Stabilizing an α/β-Hydrolase

scientific article published on 31 October 2017

Converting an esterase into an epoxide hydrolase

scientific article published in January 2009

Development of colorimetric HTS assay of cytochrome p450 for ortho-specific hydroxylation, and engineering of CYP102D1 with enhanced catalytic activity and regioselectivity

scientific article published on 18 June 2013

Developmental evolution facilitates rapid adaptation

scientific article published on 21 November 2017

Different active-site loop orientation in serine hydrolases versus acyltransferases

scientific article

Enantiocomplementary enzymes: classification, molecular basis for their enantiopreference, and prospects for mirror-image biotransformations.

scientific article published on January 2008

Engineering a multipurpose catalyst

scientific article published in October 2006

Engineering enzymes by 'intelligent' design.

scientific article published in February 2009

Engineering more stable proteins

scientific article published on 11 October 2018

Engineering the third wave of biocatalysis

scientific article

Enzymatic synthesis of poly(hydroxyalkanoates) in ionic liquids

scientific article published on 24 April 2007

Evolution of a Catalytic Mechanism.

scientific article published on 16 December 2015

Evolutionary innovation using EDGE, a system for localized elevated mutagenesis

scientific article published on 30 April 2020

Experimental Evolution of Trichoderma citrinoviride for Faster Deconstruction of Cellulose

scientific article published on 28 January 2016

Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity.

scientific article

Finding better protein engineering strategies

From nature to industry: Harnessing enzymes for biocatalysis

scientific article published on 23 November 2023

High-Level Production of Lysine in the Yeast Saccharomyces cerevisiae by Rational Design of Homocitrate Synthase

scientific article published on 13 July 2021

How substrate solvation contributes to the enantioselectivity of subtilisin toward secondary alcohols.

scientific article published in September 2005

How the Same Core Catalytic Machinery Catalyzes 17 Different Reactions: the Serine-Histidine-Aspartate Catalytic Triad of α/β-Hydrolase Fold Enzymes.

scientific article

Hydrolase-catalyzed biotransformations in deep eutectic solvents

scientific article

Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism

scientific article published on 15 May 2017

Improved preparation and use of room-temperature ionic liquids in lipase-catalyzed enantio- and regioselective acylations.

scientific article published in December 2001

Improved pretreatment of lignocellulosic biomass using enzymatically-generated peracetic acid

scientific article published on 02 February 2011

Improving enzyme properties: when are closer mutations better?

scientific article

Improving hydrolases for organic synthesis

article

Increased saccharification yields from aspen biomass upon treatment with enzymatically generated peracetic acid.

scientific article published in June 2009

Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis toward mandelonitrile by copying active site residues from an esterase that accepts aromatic esters.

scientific article

Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity

scientific article published on 30 June 2020

Manganese-substituted carbonic anhydrase as a new peroxidase

scientific article published in February 2006

Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes

Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol.

scientific article published in April 2005

Molecular Basis for Enantioselectivity of Lipase from Pseudomonas cepacia toward Primary Alcohols. Modeling, Kinetics, and Chemical Modification of Tyr29 to Increase or Decrease Enantioselectivity.

scientific article published in April 1999

Molecular basis for the stereoselective ammoniolysis of N-alkyl aziridine-2-carboxylates catalyzed by Candida antarctica lipase B.

scientific article published in September 2009

Molecular basis of perhydrolase activity in serine hydrolases.

scientific article

Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate

scientific article published in May 2003

Natural product biosynthesis: the road to L

scientific article published on 01 January 2015

New structural motif for carboxylic acid perhydrolases.

scientific article published on 16 January 2013

One-step pretreatment of yellow poplar biomass using peracetic acid to enhance enzymatic digestibility.

scientific article published on 22 September 2017

Production of 7-O-methyl aromadendrin, a medicinally valuable flavonoid, in Escherichia coli.

scientific article published on 18 November 2011

Production of p-hydroxybenzoic acid from p-coumaric acid by Burkholderia glumae BGR1.

scientific article published on 22 December 2015

Protein engineering of α/β-hydrolase fold enzymes

scientific article published on 19 April 2011

Pseudodynamic combinatorial libraries: a receptor-assisted approach for drug discovery

scientific article

Receptor-assisted combinatorial chemistry: thermodynamics and kinetics in drug discovery

scientific article

Remote interactions explain the unusual regioselectivity of lipase from Pseudomonas cepacia toward the secondary hydroxyl of 2'-deoxynucleosides.

scientific article published in April 2006

Revised molecular basis of the promiscuous carboxylic acid perhydrolase activity in serine hydrolases

scientific article published on 22 May 2012

Stabilization of an α/β-Hydrolase by Introducing Proline Residues: Salicylic Acid Binding Protein 2 from Tobacco

scientific article

Structure of an aryl esterase from Pseudomonas fluorescens.

scientific article

Subtilisin-catalyzed resolution of N-acyl arylsulfinamides.

scientific article published in February 2005

Survey of protein engineering strategies

Switching Catalysis from Hydrolysis to Perhydrolysis in Pseudomonas fluorescens Esterase ,

scientific article

Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions

scientific article

Tech.Sight. Modeling--a tool for experimentalists

scientific article

The Fungus Trichoderma Regulates Submerged Conidiation Using the Steroid Pregnenolone

scientific article published on 14 July 2016

Uncovering divergent evolution of α/β-hydrolases: a surprising residue substitution needed to convert Hevea brasiliensis hydroxynitrile lyase into an esterase

scientific article

Unexpected subtilisin-catalyzed hydrolysis of a sulfinamide bond in preference to a carboxamide bond in N-acyl sulfinamides.

scientific article published in May 2005

List of works by Romas J. Kazlauskas

Amplification of screening sensitivity through selective destruction: theory and screening of a library of carbonic anhydrase inhibitors.

An inverse substrate orientation for the regioselective acylation of 3',5'-diaminonucleosides catalyzed by Candida antarctica lipase B?

Analogs of reaction intermediates identify a unique substrate binding site in Candida rugosa lipase

Biocatalysis in ionic liquids - advantages beyond green technology

Biocatalysis--becoming more predictable and selective

Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system.

Biological chemistry: enzymes in focus

Biology evolves to fight chemistry

Biosynthesis of (-)-5-Hydroxy-equol and 5-Hydroxy-dehydroequol from Soy Isoflavone, Genistein Using Microbial Whole Cell Bioconversion

Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases

Catalytic promiscuity in biocatalysis: using old enzymes to form new bonds and follow new pathways

Chemical biotechnology in progress

Cloning, expression and characterization of CYP102D1, a self-sufficient P450 monooxygenase from Streptomyces avermitilis

Comparison of Five Protein Engineering Strategies for Stabilizing an α/β-Hydrolase

Converting an esterase into an epoxide hydrolase

Development of colorimetric HTS assay of cytochrome p450 for ortho-specific hydroxylation, and engineering of CYP102D1 with enhanced catalytic activity and regioselectivity

Developmental evolution facilitates rapid adaptation

Different active-site loop orientation in serine hydrolases versus acyltransferases

Enantiocomplementary enzymes: classification, molecular basis for their enantiopreference, and prospects for mirror-image biotransformations.

Engineering a multipurpose catalyst

Engineering enzymes by 'intelligent' design.

Engineering more stable proteins

Engineering the third wave of biocatalysis

Enzymatic synthesis of poly(hydroxyalkanoates) in ionic liquids

Evolution of a Catalytic Mechanism.

Evolutionary innovation using EDGE, a system for localized elevated mutagenesis

Experimental Evolution of Trichoderma citrinoviride for Faster Deconstruction of Cellulose

Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity.

Finding better protein engineering strategies

From nature to industry: Harnessing enzymes for biocatalysis

High-Level Production of Lysine in the Yeast Saccharomyces cerevisiae by Rational Design of Homocitrate Synthase

How substrate solvation contributes to the enantioselectivity of subtilisin toward secondary alcohols.

How the Same Core Catalytic Machinery Catalyzes 17 Different Reactions: the Serine-Histidine-Aspartate Catalytic Triad of α/β-Hydrolase Fold Enzymes.

Hydrolase-catalyzed biotransformations in deep eutectic solvents

Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism

Improved preparation and use of room-temperature ionic liquids in lipase-catalyzed enantio- and regioselective acylations.

Improved pretreatment of lignocellulosic biomass using enzymatically-generated peracetic acid

Improving enzyme properties: when are closer mutations better?

Improving hydrolases for organic synthesis

Increased saccharification yields from aspen biomass upon treatment with enzymatically generated peracetic acid.

Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis toward mandelonitrile by copying active site residues from an esterase that accepts aromatic esters.

Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity

Manganese-substituted carbonic anhydrase as a new peroxidase

Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes

Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol.

Molecular Basis for Enantioselectivity of Lipase from Pseudomonas cepacia toward Primary Alcohols. Modeling, Kinetics, and Chemical Modification of Tyr29 to Increase or Decrease Enantioselectivity.

Molecular basis for the stereoselective ammoniolysis of N-alkyl aziridine-2-carboxylates catalyzed by Candida antarctica lipase B.

Molecular basis of perhydrolase activity in serine hydrolases.

Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate

Natural product biosynthesis: the road to L

New structural motif for carboxylic acid perhydrolases.

One-step pretreatment of yellow poplar biomass using peracetic acid to enhance enzymatic digestibility.

Production of 7-O-methyl aromadendrin, a medicinally valuable flavonoid, in Escherichia coli.

Production of p-hydroxybenzoic acid from p-coumaric acid by Burkholderia glumae BGR1.

Protein engineering of α/β-hydrolase fold enzymes

Pseudodynamic combinatorial libraries: a receptor-assisted approach for drug discovery

Receptor-assisted combinatorial chemistry: thermodynamics and kinetics in drug discovery

Remote interactions explain the unusual regioselectivity of lipase from Pseudomonas cepacia toward the secondary hydroxyl of 2'-deoxynucleosides.

Revised molecular basis of the promiscuous carboxylic acid perhydrolase activity in serine hydrolases

Stabilization of an α/β-Hydrolase by Introducing Proline Residues: Salicylic Acid Binding Protein 2 from Tobacco

Structure of an aryl esterase from Pseudomonas fluorescens.

Subtilisin-catalyzed resolution of N-acyl arylsulfinamides.

Survey of protein engineering strategies

Switching Catalysis from Hydrolysis to Perhydrolysis in Pseudomonas fluorescens Esterase ,

Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions

Tech.Sight. Modeling--a tool for experimentalists

The Fungus Trichoderma Regulates Submerged Conidiation Using the Steroid Pregnenolone

Uncovering divergent evolution of α/β-hydrolases: a surprising residue substitution needed to convert Hevea brasiliensis hydroxynitrile lyase into an esterase

Unexpected subtilisin-catalyzed hydrolysis of a sulfinamide bond in preference to a carboxamide bond in N-acyl sulfinamides.