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List of works by Duarte Miguel F Prazeres

A biomolecular recognition approach for the functionalization of cellulose with gold nanoparticles

scientific article published on 17 April 2017

A multiphasic hollow fiber reactor for the whole-cell bioconversion of 2-methyl-1,3-propanediol to (r)-beta-hydroxyisobutyric acid.

scientific article published in May 2001

Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.

scientific article published on 31 July 2008

Alternatives for the intermediate recovery of plasmid DNA: performance, economic viability and environmental impact.

scientific article published in February 2009

Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events

scientific article published on 23 May 2010

Anion exchange purification of plasmid DNA using expanded bed adsorption.

scientific article published in January 2000

Application of central composite design for DNA hybridization onto magnetic microparticles

scientific article published on 10 May 2009

Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography.

scientific article

Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification.

scientific article

Biotransformation in organic media by enzymes and whole cells

article

Biotransformations in two-liquid-phase systems

Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains.

scientific article published on 21 April 2014

Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography.

scientific article published on November 2010

Characterization of the Topography and Wettability of English Weed Leaves and Biomimetic Replicas

Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles

scientific article published on 26 September 2006

Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples.

scientific article published on 15 May 2008

Chromatography of plasmid DNA.

scientific article

Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography

scientific article published on 6 September 2007

Colorimetric Detection of DNA Strands on Cellulose Microparticles Using ZZ-CBM Fusions and Gold Nanoparticles

scientific article published on 02 July 2019

Colorimetric detection of D-dimer in a paper-based immunodetection device.

scientific article

Comparative analysis of antigen-targeting sequences used in DNA vaccines.

scientific article published in March 2010

Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products.

scientific article

DNA vaccines: a rational design against parasitic diseases.

scientific article published on February 2010

De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production.

scientific article published on 12 August 2012

Density Gradient Selection of Colloidal Silver Nanotriangles for Assembling Dye-Particle Plasmophores

scientific article published on 18 June 2019

Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors

scientific article

Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes

article by Pedro Novo et al published January 2013 in Sensors and Actuators B

Development of a candidate DNA vaccine against Maedi-Visna virus.

scientific article

Development of a nicking endonuclease-assisted method for the purification of minicircles.

scientific article

Development of a phenyl membrane chromatography-based process yielding pharmaceutical grade plasmid deoxyribonucleic acid for mammalian cells transfection.

scientific article published on 18 February 2014

Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery

scientific article published on 20 January 2012

Dipeptide synthesis and separation in a reversed micellar membrane reactor

scientific article published on 01 December 1994

Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography.

scientific article

Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.

scientific article published on 14 May 2009

Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips

scientific article published on 09 August 2005

Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: simplex optimization of the biotransformation

Engineering of Escherichia coli strains for plasmid biopharmaceutical production: scale-up challenges

scientific article published on 2 March 2014

Engineering of Human Mesenchymal Stem/Stromal Cells with Vascular Endothelial Growth Factor-Encoding Minicircles for Angiogenic Ex Vivo Gene Therapy

scientific article published on 13 November 2018

Enhancement of DNA vaccine efficacy by intracellular targeting strategies

scientific article published on January 2014

Evaluation of the effect of non-B DNA structures on plasmid integrity via accelerated stability studies.

scientific article published on April 2009

Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin.

scientific article

Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters

scientific article published on 27 April 2014

Extreme Enhancement of Single-Molecule Fluorescence from Porphyrins Induced by Gold Nanodimer Antennas

scientific article published on 20 March 2019

Fluorescence correlation spectroscopy study of the complexation of DNA hybrids, IgG antibody, and a chimeric protein of IgG-binding ZZ domains fused with a carbohydrate binding module.

scientific article published on 15 June 2017

Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement

scientific article published on 01 March 2020

G protein-coupled receptors: an overview of signaling mechanisms and screening assays

scientific article published on January 2015

Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes ⬇️

scientific article published on February 15, 2011

High frequency plasmid recombination mediated by 28 bp direct repeats

scientific article published on 8 July 2008

Horseradish peroxidase: a valuable tool in biotechnology

scientific article published on January 2003

Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B

scientific article published on 01 July 2003

Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B. Application of the solvophobic theory.

scientific article published in January 2002

Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization.

scientific article published in May 2010

Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays

scientific article published on 01 July 2004

Impact of plasmid quality on lipoplex-mediated transfection

scientific article

Impact of plasmid size on the purification of model plasmid DNA vaccines by phenyl membrane adsorbers

scientific article

Improvement of DNA minicircle production by optimization of the secondary structure of the 5'-UTR of ParA resolvase

scientific article

Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors

scientific article published on 26 April 2012

Ionic Liquid-Polymer Nanoparticle Hybrid Systems as New Tools to Deliver Poorly Soluble Drugs

scientific article published on 10 August 2019

Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics

scientific article published on 24 January 2013

Microbial Stereoselective Oxidation of 2-methyl-1,3-propanediol to (R)-β-hydroxyisobutyric Acid in Aqueous/organic Biphasic Systems

article

Microspot-based ELISA in microfluidics: chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes.

scientific article published on 20 October 2011

Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters

scientific article published on 5 September 2013

Mutation detection in plasmid-based biopharmaceuticals.

scientific article published on 16 February 2011

On the dual effect of glucose during production of pBAD/AraC-based minicircles.

scientific article

On the stability of plasmid DNA vectors during cell culture and purification.

scientific article published in June 2007

Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA.

scientific article

Optimization of the primary recovery of human interferon alpha2b from Escherichia coli inclusion bodies.

scientific article

Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site

scientific article published on 20 May 2019

Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: fermentation strategies and impact on downstream processing

scientific article published in July 2014

Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase

Prediction of diffusion coefficients of plasmids

scientific article published in March 2008

Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process

scientific article published on 03 July 2007

Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography

scientific article

Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography

scientific article published on 10 August 2018

Production, purification and analysis of an experimental DNA vaccine against rabies.

scientific article published in November 2001

Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition.

scientific article published on 26 January 2012

Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic chromatography.

scientific article

Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography.

scientific article

Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography

scientific article published in August 2005

Purification of plasmids for gene therapy and DNA vaccination.

scientific article

Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma

scientific article published on 07 June 2018

Recombination frequency in plasmid DNA containing direct repeats--predictive correlation with repeat and intervening sequence length

scientific article

Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine-agarose chromatography step

scientific article published in November 2006

Separation and Analysis of Plasmid Denatured Forms Using Hydrophobic Interaction Chromatography

scientific article published on 01 November 1999

Separation of plasmid DNA topoisomers by multimodal chromatography.

scientific article

Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support

scientific article published on 25 March 2005

Single base mismatch detection by microsecond voltage pulses

scientific article published on 01 December 2005

Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography

scientific article

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C.

scientific article

Stability of free and immobilised peroxidase in aqueous–organic solvents mixtures

Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices

scientific article published on 22 June 2009

Structural instability of plasmid biopharmaceuticals: challenges and implications.

scientific article published on 03 August 2009

Studies on the Batch Adsorption of Plasmid DNA onto Anion-Exchange Chromatographic Supports

scientific article published on 01 May 2000

Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads.

scientific article

The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA

scientific article published on 05 December 2008

The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases

scientific article published in May 2004

The role of probe–probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles

scientific article published on 22 May 2009

Time-course determination of plasmid content in eukaryotic and prokaryotic cells using real-time PCR.

scientific article published in October 2007

Towards effective non-viral gene delivery vector.

scientific article published on April 2015

Towards the miniaturization of GPCR-based live-cell screening assays.

scientific article published on 23 August 2012

Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis

scientific article published on 7 July 2009

Translational features of human alpha 2b interferon production in Escherichia coli.

scientific article

Trends in dengue diagnosis.

scientific article

Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis.

scientific article published on 18 June 2010

Use of ImageJ to recover information from individual cells in a G protein-coupled receptor assay

scientific article

Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography.

scientific article published in November 2012

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