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List of works by Vladimir Besada

A computer program to aid the sequencing of peptides in collision-activated decomposition experiments.

scientific article

A deeper mining on the protein composition of VA-MENGOC-BC®: An OMV-based vaccine against N. meningitidis serogroup B and C.

scientific article published on 30 October 2017

A method for determination of N-glycosylation sites in glycoproteins by collision-induced dissociation analysis in fast atom bombardment mass spectrometry: identification of the positions of carbohydrate-linked asparagine in recombinant alpha-amylas

scientific article published on 01 August 1992

Automated interpretation of low-energy collision-induced dissociation spectra by SeqMS, a software aid for de novo sequencing by tandem mass spectrometry

scientific article published on 01 May 2000

Automated interpretation of mass spectra of complex mixtures by matching of isotope peak distributions

scientific article

Bio-analytical method based on MALDI-MS analysis for the quantification of CIGB-300 anti-tumor peptide in human plasma

scientific article published on 3 December 2014

CIGB-300, a novel proapoptotic peptide that impairs the CK2 phosphorylation and exhibits anticancer properties both in vitro and in vivo

scientific article

CIGB-300, a synthetic peptide-based drug that targets the CK2 phosphoaceptor domain. Translational and clinical research ⬇️

scientific article published on July 7, 2011

Characterization and comparative 3D modeling of CmPI-II, a novel 'non-classical' Kazal-type inhibitor from the marine snail Cenchritis muricatus (Mollusca).

scientific article published in November 2007

Characterization of low-abundance species in the active pharmaceutical ingredient of CIGB-300: A clinical-grade anticancer synthetic peptide.

scientific article

Charge state-selective separation of peptides by reversible modification of amino groups and strong cation-exchange chromatography: Evaluation in proteomic studies using peptide-centric database searches

Comparative proteomic analysis of growth hormone secretagogue A233 treatment of murine macrophage cells J774A.2 indicates it has a role in antiviral innate response

scientific article published on 14 January 2016

Computational proteomics pitfalls and challenges: HavanaBioinfo 2012 workshop report.

scientific article

Concanavalin A- and wheat germ agglutinin-conjugated lectins as a tool for the identification of multiple N-glycosylation sites in heterologous protein expressed in yeast

scientific article published on 01 November 1995

Development and validation of a bioanalytical LC-MS method for the quantification of GHRP-6 in human plasma

scientific article published on 15 November 2011

Development and validation of a bioanalytical method based on LC-MS/MS analysis for the quantitation of CIGB-814 peptide in plasma from Rheumatoid Arthritis patients

scientific article published in June 2017

Differentiating alpha- and beta-aspartic acids by electrospray ionization and low-energy tandem mass spectrometry

scientific article published on 01 January 2000

Double acylation for identification of amino-terminal peptides of proteins isolated by polyacrylamide gel electrophoresis

scientific article published in January 2007

Effect of the position of a basic amino acid on C-terminal rearrangement of protonated peptides upon collision-induced dissociation

scientific article published on 01 February 1996

Effectively addressing complex proteomic search spaces with peptide spectrum matching

scientific article published on 27 February 2013

Evaluation of phenylthiocarbamoyl-derivatized peptides by electrospray ionization mass spectrometry: selective isolation and analysis of modified multiply charged peptides for liquid chromatography-tandem mass spectrometry experiments

scientific article published in October 2010

Expression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence.

scientific article

Growth hormone-releasing peptide 6 prevents cutaneous hypertrophic scarring: early mechanistic data from a proteome study.

scientific article published on 21 February 2018

HI-bone: a scoring system for identifying phenylisothiocyanate-derivatized peptides based on precursor mass and high intensity fragment ions

scientific article published on 20 March 2013

Heberkinasa: recombinant streptokinase

scientific article published on 09 June 2005

Identification of Vimentin as a Potential Therapeutic Target against HIV Infection

scientific article published on 15 June 2016

Improving the expression of mini-proinsulin in Pichia pastoris.

scientific article published in August 2004

In silico analysis of accurate proteomics, complemented by selective isolation of peptides

article

Introducing an Asp-Pro linker in the synthesis of random one-bead-one-compound hexapeptide libraries compatible with ESI-MS analysis

scientific article

Isoelectric point optimization using peptide descriptors and support vector machines.

scientific article

Isotopica: a tool for the calculation and viewing of complex isotopic envelopes

scientific article published on July 2004

Letter: Specific isotope labeling for the identification of free N-terminal peptide of proteins separated by polyacrylamide gel electrophoresis.

scientific article published in January 2007

Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris

scientific article published in March 2005

Peptide fractionation by SDS-free polyacrylamide gel electrophoresis for proteomic analysis via DF-PAGE

scientific article published on 01 January 2012

Peptide fractionation by acid pH SDS-free electrophoresis

article published in 2011

Primary structure of two cytolysin isoforms from Stichodactyla helianthus differing in their hemolytic activity

scientific article published on 01 August 2001

Production and characterization of human gamma interferon from Escherichia coli

scientific article published on July 1, 1990

Protein content of the Hylesia metabus egg nest setae (Cramer [1775]) (Lepidoptera: Saturniidae) and its association with the parental investment for the reproductive success and lepidopterism.

scientific article

Proteomic Study to Survey the CIGB-552 Antitumor Effect

scientific article published on 20 October 2015

Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens

scientific article published on 8 May 2009

Proteomics based on peptide fractionation by SDS-free PAGE.

scientific article

Proteomics of xenografted human breast cancer indicates novel targets related to tamoxifen resistance

scientific article

Purification and characterization of two hemolysins from Stichodactyla helianthus.

scientific article

Receptor-activated human α2-macroglobulin interacts with the envelope protein of dengue virus and protects virions from temperature-induced inactivation through multivalent binding

scientific article published on 6 August 2014

Recombinant novel pituitary adenylate cyclase-activating polypeptide from African catfish (Clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates

scientific article published in June 2008

Recombinant truncated tilapia growth hormone enhances growth and innate immunity in tilapia fry (Oreochromis sp.).

scientific article published on 16 March 2008

SCAPE: a new tool for the Selective CApture of PEptides in protein identification

scientific article published in March 2005

SCX charge state selective separation of tryptic peptides combined with 2D-RP-HPLC allows for detailed proteome mapping

scientific article

Selective isolation of lysine-free tryptic peptides delimited by arginine residues: A new tool for proteome analysis

scientific article

Selective isolation of multiple positively charged peptides for 2-DE-free quantitative proteomics

scientific article published in August 2006

Selective isolation of multiply charged peptides: a confident strategy for protein identification using a linear trap quadrupole mass spectrometer

scientific article published in January 2012

Selective isolation-detection of two different positively charged peptides groups by strong cation exchange chromatography and matrix-assisted laser desorption/ionization mass spectrometry: application to proteomics studies

scientific article published on January 2010

Targeting the hydrophilic regions of recombinant proteins by MS via in-solution buffer-free trypsin digestion

scientific article published on 10 December 2019

The bovine IFN-ω1 is biologically active and secreted at high levels in the yeast Pichia pastoris ⬇️

scientific article published on February 5, 1998

The trypsin inhibitor panulirin regulates the prophenoloxidase-activating system in the spiny lobster Panulirus argus.

scientific article

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