List of works - Ricardo Flores

A chloroplastic RNA polymerase resistant to tagetitoxin is involved in replication of avocado sunblotch viroid

scientific article published on 01 March 2000

A general strategy for cloning viroids and other small circular RNAs that uses minimal amounts of template and does not require prior knowledge of its sequence

scientific article published on 01 January 1996

A kissing-loop interaction in a hammerhead viroid RNA critical for its in vitro folding and in vivo viability.

scientific article published on 31 May 2005

A naked plant-specific RNA ten-fold smaller than the smallest known viral RNA: the viroid

article

A pospiviroid from symptomless portulaca plants closely related to iresine viroid 1.

scientific article

A proposed scheme for viroid classification and nomenclature

scientific article

A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue

scientific article published on August 1, 1978

A set of novel RNAs transcribed from the chloroplast genome accumulates in date palm leaflets affected by brittle leaf disease

scientific article published in March 2008

A viroid RNA with a specific structural motif inhibits chloroplast development.

scientific article

Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection againstCitrus tristeza virusin transgenic Mexican lime

scientific article published on 01 January 2010

An extra nucleotide in the consensus catalytic core of a viroid hammerhead ribozyme: implications for the design of more efficient ribozymes.

scientific article published on 13 July 2001

Analysis of viroid replication.

scientific article

Callose Deposition in Plasmodesmata and Viroid Invasion of the Shoot Apical Meristem

scientific article

Characterization of RNAs specific to avocado sunblotch viroid synthesized in vitro by a cell-free system from infected avocado leaves

scientific article published on 01 February 1992

Characterization of viroid-like RNAs associated with the citrus exocortis syndrome

scientific article published on 01 April 1986

Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. II. Characterization of a new species in the genus Partitivirus.

scientific article

Chrysanthemum chlorotic mottle viroid: unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes.

scientific article published on October 1997

Citrus tristeza virus p23: a unique protein mediating key virus-host interactions.

scientific article published on 3 May 2013

Citrus tristeza virus p23: determinants for nucleolar localization and their influence on suppression of RNA silencing and pathogenesis.

scientific article

Citrus viroid V: occurrence, host range, diagnosis, and identification of new variants

scientific article

Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization

scientific article published on 01 January 1999

Current status of viroid taxonomy

scientific article

Cytopathic Effects Incited by Viroid RNAs and Putative Underlying Mechanisms.

scientific article published on January 2012

Dahlia latent viroid: a recombinant new species of the family Pospiviroidae posing intriguing questions about its origin and classification.

scientific article

Deep sequencing of the small RNAs derived from two symptomatic variants of a chloroplastic viroid: implications for their genesis and for pathogenesis

scientific article

Detection of avocado sunblotch viroid in chloroplasts of avocado leaves by in situ hybridization

scientific article published on 01 January 1994

Detection of citrus exocortis viroid in crude extracts by dot-blot hybridization: conditions for reducing spurious hybridization results and for enhancing the sensitivity of the technique

scientific article published on 01 May 1986

Diagnosis of "Maladie des feuilles cassantes" or Brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs

scientific article published on 19 May 2006

Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing

scientific article published on 14 September 2017

Direct visualization of the native structure of viroid RNAs at single-molecule resolution by atomic force microscopy

scientific article published on 08 February 2019

Dissecting the secondary structure of the circular RNA of a nuclear viroid in vivo: A "naked" rod-like conformation similar but not identical to that observed in vitro.

scientific article published on 30 August 2016

Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families

scientific article published on 29 October 2007

Ectopic expression of the p23 silencing suppressor of Citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts

scientific article published on 30 May 2011

Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: differences in co- and post-transcriptional self-cleavage may explain the lack of trinucleotide AUC in most natural hammerheads

scientific article published on 5 October 2006

Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids)

scientific article

Engineering resistance against viroids.

scientific article

Existence in vivo of the loop E motif in potato spindle tuber viroid RNA

scientific article published on 19 March 2007

Extremely high mutation rate of a hammerhead viroid

scientific article

Fractionation with ethanol of nucleic acids from viroid-infected plants

scientific article published on 01 October 1983

Hammerhead Ribozymes Against Virus and Viroid RNAs

article

Hammerhead ribozyme structure and function in plant RNA replication.

scientific article published in January 2001

Highly Abundant Small Interfering RNAs Derived from a Satellite RNA Contribute to Symptom Attenuation by Binding Helper Virus-Encoded RNA Silencing Suppressors.

scientific article

How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host

scientific article published on 16 April 2019

Identification in eggplant of a variant of citrus exocortis viroid (CEVd) with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure.

scientific article

In vitro and in vivo self-cleavage of a viroid RNA with a mutation in the hammerhead catalytic pocket.

scientific article published on April 1998

Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection.

scientific article published on 17 February 2017

Involvement of the chloroplastic isoform of tRNA ligase in the replication of viroids belonging to the family Avsunviroidae.

scientific article published on 23 May 2012

Mapping the molecular determinant of pathogenicity in a hammerhead viroid: a tetraloop within the in vivo branched RNA conformation.

scientific article

Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology.

scientific article published in October 2006

Monomeric linear RNA of citrus exocortis viroid resulting from processing in vivo has 5'-phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase involved in replication.

scientific article published on 13 August 2008

Next-Generation Sequencing and Genome Editing in Plant Virology

scientific article

Pathogenesis by subviral agents: viroids and hepatitis delta virus

scientific article

Peach latent mosaic viroid variants inducing peach calico (extreme chlorosis) contain a characteristic insertion that is responsible for this symptomatology.

scientific article published in September 2003

Pepper chat fruit viroid: biological and molecular properties of a proposed new species of the genus Pospiviroid.

scientific article

Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity ⬇️

scientific article published on October 15, 2003

Plus and minus RNAs of peach latent mosaic viroid self-cleave in vitro via hammerhead structures ⬇️

scientific article published on May 1, 1992

Polymorphism of a specific region in gene p23 of Citrus tristeza virus allows discrimination between mild and severe isolates

scientific article published on 16 September 2003

Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime

scientific article

Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts.

scientific article published on 7 February 2007

Processing of nuclear viroids in vivo: an interplay between RNA conformations.

scientific article

RNA-dependent RNA polymerase 6 delays accumulation and precludes meristem invasion of a viroid that replicates in the nucleus

scientific article

Replication of avocado sunblotch viroid: evidence for a symmetric pathway with two rolling circles and hammerhead ribozyme processing.

scientific article

Reverse transcription polymerase chain reaction protocols for cloning small circular RNAs.

scientific article

Revisiting the cysteine-rich proteins encoded in the 3'-proximal open reading frame of the positive-sense single-stranded RNA of some monopartite filamentous plant viruses: functional dissection of p15 from grapevine virus B

scientific article published on 16 July 2020

Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA

scientific article published on 14 December 2010

Rolling-circle replication of viroids, viroid-like satellite RNAs and hepatitis delta virus: variations on a theme.

scientific article published on March 2011

Sequences of Citrus tristeza virus separated in time and space are essentially identical.

scientific article published in August 2000

Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing.

scientific article

Some properties of the viroid inducing peach latent mosaic disease.

scientific article published in January 1990

Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo

scientific article

Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads

scientific article

Symptoms induced by transgenic expression of p23 from Citrus tristeza virus in phloem-associated cells of Mexican lime mimic virus infection without the aberrations accompanying constitutive expression

scientific article published on October 2014

The 23-kDa protein coded by the 3'-terminal gene of citrus tristeza virus is an RNA-binding protein

scientific article published in April 2000

The DNA of a plant retroviroid-like element is fused to different sites in the genome of a plant pararetrovirus and shows multiple forms with sequence deletions.

scientific article published on November 2000

The conserved structures of the 5' nontranslated region of Citrus tristeza virus are involved in replication and virion assembly

scientific article published in December 2003

The predominant circular form of avocado sunblotch viroid accumulates in planta as a free RNA adopting a rod-shaped secondary structure unprotected by tightly bound host proteins.

scientific article published on 12 July 2017

The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways.

scientific article published on 2 September 2016

The transcription initiation sites of eggplant latent viroid strands map within distinct motifs in their in vivo RNA conformations

scientific article

Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA

scientific article published on 21 November 2010

Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus

scientific article

Two chloroplastic viroids induce the accumulation of small RNAs associated with posttranscriptional gene silencing.

scientific article published on December 2002

Variants of Peach latent mosaic viroid inducing peach calico: uneven distribution in infected plants and requirements of the insertion containing the pathogenicity determinant

scientific article

Viral-like symptoms induced by the ectopic expression of the p23 gene of Citrus tristeza virus are citrus specific and do not correlate with the pathogenicity of the virus strain

scientific article published in May 2005

Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase

scientific article

Viroid RNA turnover: characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo.

scientific article published on 6 February 2015

Viroid replication: rolling-circles, enzymes and ribozymes

scientific article

Viroids and viroid-host interactions.

scientific article

Viroids, the simplest RNA replicons: How they manipulate their hosts for being propagated and how their hosts react for containing the infection

scientific article

Viroids: The Noncoding Genomes

article

Viroids: an Ariadne's thread into the RNA labyrinth

scientific article

Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs

scientific article published on 18 June 2012

Viroids: how to infect a host and cause disease without encoding proteins.

scientific article published on 23 February 2012

Viroids: survivors from the RNA world?

scientific article (publication date: 8 September 2014)

Viroids: the minimal non-coding RNAs with autonomous replication

scientific article published on June 2004

e-Book on Closteroviridae

scientific article published in 2013

List of works by Ricardo Flores

A chloroplastic RNA polymerase resistant to tagetitoxin is involved in replication of avocado sunblotch viroid

A general strategy for cloning viroids and other small circular RNAs that uses minimal amounts of template and does not require prior knowledge of its sequence

A kissing-loop interaction in a hammerhead viroid RNA critical for its in vitro folding and in vivo viability.

A naked plant-specific RNA ten-fold smaller than the smallest known viral RNA: the viroid

A pospiviroid from symptomless portulaca plants closely related to iresine viroid 1.

A proposed scheme for viroid classification and nomenclature

A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue

A set of novel RNAs transcribed from the chloroplast genome accumulates in date palm leaflets affected by brittle leaf disease

A viroid RNA with a specific structural motif inhibits chloroplast development.

Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection againstCitrus tristeza virusin transgenic Mexican lime

An extra nucleotide in the consensus catalytic core of a viroid hammerhead ribozyme: implications for the design of more efficient ribozymes.

Analysis of viroid replication.

Callose Deposition in Plasmodesmata and Viroid Invasion of the Shoot Apical Meristem

Characterization of RNAs specific to avocado sunblotch viroid synthesized in vitro by a cell-free system from infected avocado leaves

Characterization of viroid-like RNAs associated with the citrus exocortis syndrome

Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. II. Characterization of a new species in the genus Partitivirus.

Chrysanthemum chlorotic mottle viroid: unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes.

Citrus tristeza virus p23: a unique protein mediating key virus-host interactions.

Citrus tristeza virus p23: determinants for nucleolar localization and their influence on suppression of RNA silencing and pathogenesis.

Citrus viroid V: occurrence, host range, diagnosis, and identification of new variants

Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization

Current status of viroid taxonomy

Cytopathic Effects Incited by Viroid RNAs and Putative Underlying Mechanisms.

Dahlia latent viroid: a recombinant new species of the family Pospiviroidae posing intriguing questions about its origin and classification.

Deep sequencing of the small RNAs derived from two symptomatic variants of a chloroplastic viroid: implications for their genesis and for pathogenesis

Detection of avocado sunblotch viroid in chloroplasts of avocado leaves by in situ hybridization

Detection of citrus exocortis viroid in crude extracts by dot-blot hybridization: conditions for reducing spurious hybridization results and for enhancing the sensitivity of the technique

Diagnosis of "Maladie des feuilles cassantes" or Brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs

Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing

Direct visualization of the native structure of viroid RNAs at single-molecule resolution by atomic force microscopy

Dissecting the secondary structure of the circular RNA of a nuclear viroid in vivo: A "naked" rod-like conformation similar but not identical to that observed in vitro.

Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families

Ectopic expression of the p23 silencing suppressor of Citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts

Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: differences in co- and post-transcriptional self-cleavage may explain the lack of trinucleotide AUC in most natural hammerheads

Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids)

Engineering resistance against viroids.

Existence in vivo of the loop E motif in potato spindle tuber viroid RNA

Extremely high mutation rate of a hammerhead viroid

Fractionation with ethanol of nucleic acids from viroid-infected plants

Hammerhead Ribozymes Against Virus and Viroid RNAs

Hammerhead ribozyme structure and function in plant RNA replication.

Highly Abundant Small Interfering RNAs Derived from a Satellite RNA Contribute to Symptom Attenuation by Binding Helper Virus-Encoded RNA Silencing Suppressors.

How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host

Identification in eggplant of a variant of citrus exocortis viroid (CEVd) with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure.

In vitro and in vivo self-cleavage of a viroid RNA with a mutation in the hammerhead catalytic pocket.

Interference between variants of peach latent mosaic viroid reveals novel features of its fitness landscape: implications for detection.

Involvement of the chloroplastic isoform of tRNA ligase in the replication of viroids belonging to the family Avsunviroidae.

Mapping the molecular determinant of pathogenicity in a hammerhead viroid: a tetraloop within the in vivo branched RNA conformation.

Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology.

Monomeric linear RNA of citrus exocortis viroid resulting from processing in vivo has 5'-phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase involved in replication.

Next-Generation Sequencing and Genome Editing in Plant Virology

Pathogenesis by subviral agents: viroids and hepatitis delta virus

Peach latent mosaic viroid variants inducing peach calico (extreme chlorosis) contain a characteristic insertion that is responsible for this symptomatology.

Pepper chat fruit viroid: biological and molecular properties of a proposed new species of the genus Pospiviroid.

Peripheral regions of natural hammerhead ribozymes greatly increase their self-cleavage activity ⬇️

Plus and minus RNAs of peach latent mosaic viroid self-cleave in vitro via hammerhead structures ⬇️

Polymorphism of a specific region in gene p23 of Citrus tristeza virus allows discrimination between mild and severe isolates

Post-transcriptional gene silencing of the p23 silencing suppressor of Citrus tristeza virus confers resistance to the virus in transgenic Mexican lime

Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts.

Processing of nuclear viroids in vivo: an interplay between RNA conformations.

RNA-dependent RNA polymerase 6 delays accumulation and precludes meristem invasion of a viroid that replicates in the nucleus

Replication of avocado sunblotch viroid: evidence for a symmetric pathway with two rolling circles and hammerhead ribozyme processing.

Reverse transcription polymerase chain reaction protocols for cloning small circular RNAs.

Revisiting the cysteine-rich proteins encoded in the 3'-proximal open reading frame of the positive-sense single-stranded RNA of some monopartite filamentous plant viruses: functional dissection of p15 from grapevine virus B

Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA

Rolling-circle replication of viroids, viroid-like satellite RNAs and hepatitis delta virus: variations on a theme.

Sequences of Citrus tristeza virus separated in time and space are essentially identical.

Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing.

Some properties of the viroid inducing peach latent mosaic disease.

Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo

Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads

Symptoms induced by transgenic expression of p23 from Citrus tristeza virus in phloem-associated cells of Mexican lime mimic virus infection without the aberrations accompanying constitutive expression

The 23-kDa protein coded by the 3'-terminal gene of citrus tristeza virus is an RNA-binding protein

The DNA of a plant retroviroid-like element is fused to different sites in the genome of a plant pararetrovirus and shows multiple forms with sequence deletions.

The conserved structures of the 5' nontranslated region of Citrus tristeza virus are involved in replication and virion assembly

The predominant circular form of avocado sunblotch viroid accumulates in planta as a free RNA adopting a rod-shaped secondary structure unprotected by tightly bound host proteins.

The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways.

The transcription initiation sites of eggplant latent viroid strands map within distinct motifs in their in vivo RNA conformations

Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA