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List of works by Warren Wakarchuk

A Bacterial Expression Platform for Production of Therapeutic Proteins Containing Human-like O-Linked Glycans

scientific article published on 29 November 2018

A Campylobacter jejuni gene associated with immune-mediated neuropathy

article

A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis.

scientific article

A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis

scientific article published in October 2013

A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase

scientific article published on May 2009

A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis

scientific article published on 10 October 2013

A sensitive fluorescence-based assay for monitoring GM2 ganglioside hydrolysis in live patient cells and their lysates

scientific article

A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni

scientific article

Allylmalonamide as a bivalent linker: synthesis of biantennary GM3-saccharide--keyhole limpet hemocyanin glycoconjugate and the immune response in mice

scientific article published on 01 September 1999

Alternative donor substrates for inverting and retaining glycosyltransferases

scientific article published on 07 November 2006

Amino acid sequence and thermostability of xylanase A from Schizophyllum commune

scientific article published on 01 November 1993

Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses

scientific article published on January 2005

Analysis of bacterial glycolipids by capillary electrophoresis-electrospray mass spectrometry: Haemophilus influenzae and Neisseria meningitidis lipopolysaccharides

scientific article published on 01 January 2003

Biochemical characterization of a polysialyltransferase from Mannheimia haemolytica A2 and comparison to other bacterial polysialyltransferases.

scientific article

Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-mhz (1)h and (13)c NMR analysis.

scientific article published on February 2000

Biosynthesis of the polymannose lipopolysaccharide O-antigens from Escherichia coli serotypes O8 and O9a requires a unique combination of single- and multiple-active site mannosyltransferases

scientific article

Characterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.

scientific article

Characterization of a recombinant Neisseria meningitidis alpha-2,3-sialyltransferase and its acceptor specificity

scientific article published on 01 October 1997

Characterization of five β-glycoside hydrolases from Cellulomonas fimi ATCC 484.

scientific article

Characterization of the alpha-1,2-N-acetylglucosaminyltransferase of Neisseria gonorrhoeae, a key control point in lipooligosaccharide biosynthesis.

scientific article published on 24 March 2004

Characterization of the alpha-2,8-polysialyltransferase from Neisseria meningitidis with synthetic acceptors, and the development of a self-priming polysialyltransferase fusion enzyme

scientific article published on 13 November 2007

Characterization of α2,3- and α2,6-sialyltransferases from Helicobacter acinonychis ⬇️

scientific article published on April 14, 2012

Chemical and chemoenzymatic synthesis of S-linked ganglioside analogues and their protein conjugates for use as immunogens

scientific article

Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces

scientific article published on 29 August 2008

Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.

scientific article published in September 2005

Cloning of the lipooligosaccharide alpha-2,3-sialyltransferase from the bacterial pathogens Neisseria meningitidis and Neisseria gonorrhoeae.

scientific article published in November 1996

Comparison of α2,6-sialyltransferases for sialylation of therapeutic proteins

scientific article published on 01 September 2019

Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida.

scientific article

Construction of a hybrid β-hexosaminidase subunit capable of forming stable homodimers that hydrolyze GM2 ganglioside in vivo

scientific article published on 2 March 2016

Development of an on-line preconcentration method for the analysis of pathogenic lipopolysaccharides using capillary electrophoresis-electrospray mass spectrometry. Application to small colony isolates

scientific article published on 01 August 1998

Directed evolution of bacterial polysialyltransferases

scientific article published on 01 July 2019

Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens

scientific article

Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.

scientific article

Enzymatic Engineering of Polysialic Acid on Cells in Vitro and in Vivo Using a Purified Bacterial Polysialyltransferase ⬇️

scientific article published on July 31, 2012

Enzymatic synthesis and properties of glycoconjugates with legionaminic acid as a replacement for neuraminic acid

scientific article published on 25 October 2010

Evidence for acquisition of the lipooligosaccharide biosynthesis locus in Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barré syndrome, by horizontal exchange

scientific article published on February 2004

Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6

scientific article published on 01 June 2000

FlaA1, a new bifunctional UDP-GlcNAc C6 Dehydratase/ C4 reductase from Helicobacter pylori

scientific article published on 01 November 2000

Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases.

scientific article

Functional characterization of dehydratase/aminotransferase pairs from Helicobacter and Campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways.

scientific article

Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis

scientific article published on 01 August 1996

Genetics and molecular specificity of sialylation of Histophilus somni lipooligosaccharide (LOS) and the effect of LOS sialylation on Toll-like receptor-4 signaling ⬇️

scientific article published on March 5, 2011

Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli

scientific article published on 26 March 2019

Glycosyl fluorides can function as substrates for nucleotide phosphosugar-dependent glycosyltransferases

scientific article published on 01 December 1999

High-throughput screening methodology for the directed evolution of glycosyltransferases

scientific article

Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase.

scientific article

Identification and characterization of a lipopolysaccharide α,2,3-sialyltransferase from the human pathogen Helicobacter bizzozeronii

scientific article

Identification of a bifunctional lipopolysaccharide sialyltransferase in Haemophilus influenzae: incorporation of disialic acid

scientific article published on 27 October 2006

Identification of a lipopolysaccharide alpha-2,3-sialyltransferase from Haemophilus influenzae.

scientific article

In cellulo examination of a beta-alpha hybrid construct of beta-hexosaminidase A subunits, reported to interact with the GM2 activator protein and hydrolyze GM2 ganglioside

scientific article

Incorporation of N-Acetylneuraminic Acid into Haemophilus somnus Lipooligosaccharide (LOS): Enhancement of Resistance to Serum and Reduction of LOS Antibody Binding.

scientific article published in November 2002

Incorporation of N-acetylneuraminic acid into Haemophilus somnus lipooligosaccharide (LOS): enhancement of resistance to serum and reduction of LOS antibody binding.

scientific article

Inhibition of galactosyltransferases by a novel class of donor analogues

scientific article published on 22 February 2012

Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue

scientific article

Large-scale in vivo synthesis of the carbohydrate moieties of gangliosides GM1 and GM2 by metabolically engineered Escherichia coli

scientific article published in May 2003

Mammalian sialyltransferases allow efficient <i>Escherichia coli</i>-based production of mucin-type O-glycoproteins but can also transfer Kdo

scientific article published in 2021

Mechanism of stabilization of Bacillus circulans xylanase upon the introduction of disulfide bonds

scientific article published on 25 October 2006

Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6.

scientific article

Mechanistic studies of a retaining alpha-galactosyltransferase from Neisseria meningitidis.

scientific article published in April 2002

NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base

scientific article

Novel biosynthetic functions of lipopolysaccharide rfaJ homologs from Helicobacter pylori

scientific article

One step closer to a sweet conclusion.

scientific article

Overexpression of the Bacillus subtilis and circulans xylanases in Escherichia coli.

scientific article

Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison

scientific article published on 01 August 2000

Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase

scientific article published on 01 February 1997

Preparative-scale chemoenzymatic synthesis of large carbohydrate assemblies using alpha (1-->4)-galactosyltransferase/UDP-4'-Gal-epimerase fusion protein

scientific article published on 01 January 2003

Proteomic Analysis of the Secretome of Cellulomonas fimi ATCC 484 and Cellulomonas flavigena ATCC 482.

scientific article

Recognition of protein-linked glycans as a determinant of peptidase activity

scientific article published on 17 January 2017

Recognition of sialylated poly-N-acetyllactosamine chains on N- and O-linked glycans by human and avian influenza A virus hemagglutinins.

scientific article

Review of phosphocholine substituents on bacterial pathogen glycans: synthesis, structures and interactions with host proteins.

scientific article published on August 2013

Sialyltransferases with enhanced legionaminic acid transferase activity for the preparation of analogs of sialoglycoconjugates

scientific article published on 03 April 2015

Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes

scientific article

Small molecules containing hetero-bicyclic ring systems compete with UDP-Glc for binding to WaaG glycosyltransferase

scientific article published on 19 June 2012

Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms

scientific article

Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog

scientific article

Structural and Kinetic Analysis of Substrate Binding to the Sialyltransferase Cst-II from Campylobacter jejuni

scientific article

Structural and Kinetic Characterizations of the Polysialic Acid O-Acetyltransferase OatWY from Neisseria meningitidis ⬇️

scientific article

Structural and functional characterization of PseC, an aminotransferase involved in the biosynthesis of pseudaminic acid, an essential flagellar modification in Helicobacter pylori

scientific article

Structure and Mechanism of the Lipooligosaccharide Sialyltransferase from Neisseria meningitidis

scientific article

Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation

scientific article

Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I.

scientific article published on 8 January 2013

Structures of Merkel Cell Polyomavirus VP1 Complexes Define a Sialic Acid Binding Site Required for Infection

scientific article

The genetic bases for the variation in the lipo-oligosaccharide of the mucosal pathogen, Campylobacter jejuni. Biosynthesis of sialylated ganglioside mimics in the core oligosaccharide

scientific article published on 31 October 2001

The living factory: in vivo production of N-acetyllactosamine containing carbohydrates in E. coli

scientific article published on 01 March 1999

The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion

scientific article published in August 1998

Thiogalactopyranosides are Resistant to Hydrolysis by α‐Galactosidases ⬇️

scientific article published on June 27, 2012

Transition state analysis of Vibrio cholerae sialidase-catalyzed hydrolyses of natural substrate analogues

scientific article published on 17 February 2012

Two GC-rich DNA elements ofChlamydomonas reinhardtii with complex arrangements of directly repeated sequence motifs ⬇️

scientific article published on January 1, 1992

Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis

scientific article published on 22 October 2006

Variants of the beta 1,3-galactosyltransferase CgtB from the bacterium Campylobacter jejuni have distinct acceptor specificities

scientific article

X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid

scientific article

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