List of works - Arthur L. Horwich

A carboxy-terminal deletion impairs the assembly of GroEL and confers a pleiotropic phenotype in Escherichia coli K-12.

scientific article

A mutant chaperonin with rearranged inter-ring electrostatic contacts and temperature-sensitive dissociation

scientific article

A small molecule inhibitor selective for a variant ATP-binding site of the chaperonin GroEL.

scientific article published on 7 December 2008

A two-domain folding intermediate of RuBisCO in complex with the GroEL chaperonin

scholarly article by Ramanathan Natesh published in October 2018

ATP-Bound States of GroEL Captured by Cryo-Electron Microscopy ⬇️

scientific article published on December 28, 2001

ATP-Triggered Conformational Changes Delineate Substrate-Binding and -Folding Mechanics of the GroEL Chaperonin

scientific article

ATP-triggered ADP release from the asymmetric chaperonin GroEL/GroES/ADP7 is not the rate-limiting step of the GroEL/GroES reaction cycle

scientific article

Absence of lipofuscin in motor neurons of SOD1-linked ALS mice

scientific article

Allosteric signaling of ATP hydrolysis in GroEL-GroES complexes

scientific article published on 22 January 2006

An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons

scientific article

An ALS-linked mutant SOD1 produces a locomotor defect associated with aggregation and synaptic dysfunction when expressed in neurons of Caenorhabditis elegans

scientific article

Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space

scientific article

Arginine in the leader peptide is required for both import and proteolytic cleavage of a mitochondrial precursor ⬇️

scientific article published on August 1985

Chaperoned protein disaggregation--the ClpB ring uses its central channel

scientific article

Chaperonin chamber accelerates protein folding through passive action of preventing aggregation

scientific article

Chaperonin studies: faith, luck, and a little help from our friends

scientific article published in November 2017

Chaperonin-assisted protein folding: a chronologue

scientific article published on 19 February 2020

Chaperonin-mediated protein folding: fate of substrate polypeptide

scientific article

Chaperonin-mediated protein folding: using a central cavity to kinetically assist polypeptide chain folding

scientific article published on May 2009

ClpS, a substrate modulator of the ClpAP machine

scientific article

Direct NMR observation of a substrate protein bound to the chaperonin GroEL.

scientific article

Disulfide formation as a probe of folding in GroEL-GroES reveals correct formation of long-range bonds and editing of incorrect short-range ones

scientific article

Double mutant MBP refolds at same rate in free solution as inside the GroEL/GroES chaperonin chamber when aggregation in free solution is prevented ⬇️

scientific article published on May 20, 2011

Exploring the structural dynamics of the E.coli chaperonin GroEL using translation-libration-screw crystallographic refinement of intermediate states

scientific article

Expression and Stabilization of Microinjected Plasmids Containing the Herpes Simplex Virus Thymidine Kinase Gene and Polyoma Virus DNA in Mouse Cells ⬇️

scientific article published on April 1, 1983

Expression of amplified DNA sequences for ornithine transcarbamylase in HeLa cells: arginine residues may be required for mitochondrial import of enzyme precursor

scientific article

Extended survival of misfolded G85R SOD1-linked ALS mice by transgenic expression of chaperone Hsp110

scientific article published on 25 April 2016

Folding in vivo of a newly translated yeast cytosolic enzyme is mediated by the SSA class of cytosolic yeast Hsp70 proteins ⬇️

scientific article published on October 27, 1998

Folding trajectories of human dihydrofolate reductase inside the GroEL GroES chaperonin cavity and free in solution

scholarly article

Folding with and without encapsulation by cis- and trans-only GroEL-GroES complexes

scientific article published on July 2003

Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL.

scholarly article

GroEL-GroES-mediated protein folding

scientific article

GroEL/ES inhibitors as potential antibiotics

scientific article published on 4 May 2016

GroEL/GroES cycling: ATP binds to an open ring before substrate protein favoring protein binding and production of the native state

scientific article

Hepadnavirus envelope proteins regulate covalently closed circular DNA amplification

scientific article published on June 1990

Hsp110 mitigates α-synuclein pathology in vivo

scientific article published on 04 November 2019

Hydrogen–deuterium exchange in vivo to measure turnover of an ALS‐associated mutant SOD1 protein in spinal cord of mice ⬇️

scientific article published on August 18, 2011

Import and processing of human ornithine transcarbamoylase precursor by mitochondria from Saccharomyces cerevisiae

scientific article

Inherited hepatic enzyme defects as candidates for liver-directed gene therapy

scientific article published on January 1991

Linkage mapping of a mouse gene, iv, that controls left-right asymmetry of the heart and viscera

scientific article

Localization of GroEL determined by in vivo incorporation of a fluorescent amino acid

scientific article

Loops in the central channel of ClpA chaperone mediate protein binding, unfolding, and translocation

scientific article published in July 2005

Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT

scientific article

Meiotic expression of human ornithine transcarbamylase in the testes of transgenic mice

scientific article published on April 1988

Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria

scientific article

Mitochondrial protein import

scientific article published on January 1991

Molecular chaperone Hsp110 rescues a vesicle transport defect produced by an ALS-associated mutant SOD1 protein in squid axoplasm

scientific article

Molecular chaperones in biology and medicine at Obernai

scientific article

Molecular chaperones in cellular protein folding: the birth of a field.

scientific article

Molecular cloning of the cDNA coding for rat ornithine transcarbamoylase

scientific article published on July 1983

Multiple states of a nucleotide-bound group 2 chaperonin

scientific article published on April 2008

NMR analysis of a 900K GroEL GroES complex

scientific article (publication date: 11 July 2002)

Native-like structure of a protein-folding intermediate bound to the chaperonin GroEL.

scientific article

No evidence for a forced-unfolding mechanism during ATP/GroES binding to substrate-bound GroEL: no observable protection of metastable Rubisco intermediate or GroEL-bound Rubisco from tritium exchange

scientific article published in February 2005

Nuclear magnetic resonance spectroscopy with the stringent substrate rhodanese bound to the single-ring variant SR1 of the E. coli chaperonin GroEL.

scientific article

Perturbed ATPase activity and not "close confinement" of substrate in the cis cavity affects rates of folding by tail-multiplied GroEL.

scholarly article

Prevention of protein denaturation under heat stress by the chaperonin Hsp60

scientific article published on November 6, 1992

Probing the sequence of conformationally induced polarity changes in the molecular chaperonin GroEL with fluorescence spectroscopy

scientific article

Production of RNA for transcriptomic analysis from mouse spinal cord motor neuron cell bodies by laser capture microdissection

scientific article

Progressive aggregation despite chaperone associations of a mutant SOD1-YFP in transgenic mice that develop ALS.

scientific article

Protein aggregation in disease: a role for folding intermediates forming specific multimeric interactions

scientific article

Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis

scientific article published in Nature

Protein folding in the cell: an inside story ⬇️

scientific article published on October 11, 2011

Protein folding in the cell: functions of two families of molecular chaperone, hsp 60 and TF55-TCP1

scientific article

Proton-proton Overhauser NMR spectroscopy with polypeptide chains in large structures

scientific article

QnAs with Arthur L. Horwich. Interview by Prashant Nair

scientific article published on 17 January 2012

RNA-Seq profiling of spinal cord motor neurons from a presymptomatic SOD1 ALS mouse

scientific article

Recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 leads to early-onset progressive neurodegeneration

scientific article

Reduced high-frequency motor neuron firing, EMG fractionation, and gait variability in awake walking ALS mice

scientific article

Requirement for binding multiple ATPs to convert a GroEL ring to the folding-active state

scholarly article

Residues in chaperonin GroEL required for polypeptide binding and release

scientific article (publication date: 13 October 1994)

Role of the -phosphate of ATP in triggering protein folding by GroEL-GroES: function, structure and energetics

scientific article

Roles of the N-domains of the ClpA unfoldase in binding substrate proteins and in stable complex formation with the ClpP protease

scientific article

Selective degeneration of a physiological subtype of spinal motor neuron in mice with SOD1-linked ALS

scientific article

Solution NMR techniques for large molecular and supramolecular structures

scientific article

Structure and Expression of a Complementary DNA for the Nuclear Coded Precursor of Human Mitochondrial Ornithine Transcarbamylase ⬇️

scientific article published on June 8, 1984

Structure and allostery of the chaperonin GroEL.

scientific article

Substrate polypeptide presents a load on the apical domains of the chaperonin GroEL.

scholarly article

Synthesis of hepadnavirus particles that contain replication-defective duck hepatitis B virus genomes in cultured HuH7 cells

scientific article published on February 1990

TCP1 complex is a molecular chaperone in tubulin biogenesis

scientific journal article

Targeting of nuclear-encoded proteins to the mitochondrial matrix: implications for human genetic defects

scientific article

Targeting the HSP60/10 chaperonin systems of Trypanosoma brucei as a strategy for treating African sleeping sickness

scientific article

The GroEL/GroES cis cavity as a passive anti-aggregation device

scientific article published on 03 July 2009

The crystal structure of the asymmetric GroEL–GroES–(ADP)7 chaperonin complex ⬇️

scientific article published on August 21, 1997

The crystal structure of the bacterial chaperonin GroEL at 2.8 A

scientific article

The mitochondrial chaperonin hsp60 is required for its own assembly

scientific article

The ornithine transcarbamylase leader peptide directs mitochondrial import through both its midportion structure and net positive charge

scientific article

Topologies of a substrate protein bound to the chaperonin GroEL

scientific article published in May 2007

Transfer of pathogenic and nonpathogenic cytosolic proteins between spinal cord motor neurons in vivo in chimeric mice

scientific article

Translational diffusion of macromolecular assemblies measured using transverse-relaxation-optimized pulsed field gradient NMR

scientific article published on 26 September 2011

Two families of chaperonin: physiology and mechanism

scientific article

Unfolded DapA forms aggregates when diluted into free solution, confounding comparison with folding by the GroEL/GroES chaperonin system

scientific article

Uniform and residue-specific 15N-labeling of proteins on a highly deuterated background

scientific article

List of works by Arthur L. Horwich

A carboxy-terminal deletion impairs the assembly of GroEL and confers a pleiotropic phenotype in Escherichia coli K-12.

A mutant chaperonin with rearranged inter-ring electrostatic contacts and temperature-sensitive dissociation

A small molecule inhibitor selective for a variant ATP-binding site of the chaperonin GroEL.

A two-domain folding intermediate of RuBisCO in complex with the GroEL chaperonin

ATP-Bound States of GroEL Captured by Cryo-Electron Microscopy ⬇️

ATP-Triggered Conformational Changes Delineate Substrate-Binding and -Folding Mechanics of the GroEL Chaperonin

ATP-triggered ADP release from the asymmetric chaperonin GroEL/GroES/ADP7 is not the rate-limiting step of the GroEL/GroES reaction cycle

Absence of lipofuscin in motor neurons of SOD1-linked ALS mice

Allosteric signaling of ATP hydrolysis in GroEL-GroES complexes

An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons

An ALS-linked mutant SOD1 produces a locomotor defect associated with aggregation and synaptic dysfunction when expressed in neurons of Caenorhabditis elegans

Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space

Arginine in the leader peptide is required for both import and proteolytic cleavage of a mitochondrial precursor ⬇️

Chaperoned protein disaggregation--the ClpB ring uses its central channel

Chaperonin chamber accelerates protein folding through passive action of preventing aggregation

Chaperonin studies: faith, luck, and a little help from our friends

Chaperonin-assisted protein folding: a chronologue

Chaperonin-mediated protein folding: fate of substrate polypeptide

Chaperonin-mediated protein folding: using a central cavity to kinetically assist polypeptide chain folding

ClpS, a substrate modulator of the ClpAP machine

Direct NMR observation of a substrate protein bound to the chaperonin GroEL.

Disulfide formation as a probe of folding in GroEL-GroES reveals correct formation of long-range bonds and editing of incorrect short-range ones

Double mutant MBP refolds at same rate in free solution as inside the GroEL/GroES chaperonin chamber when aggregation in free solution is prevented ⬇️

Exploring the structural dynamics of the E.coli chaperonin GroEL using translation-libration-screw crystallographic refinement of intermediate states

Expression and Stabilization of Microinjected Plasmids Containing the Herpes Simplex Virus Thymidine Kinase Gene and Polyoma Virus DNA in Mouse Cells ⬇️

Expression of amplified DNA sequences for ornithine transcarbamylase in HeLa cells: arginine residues may be required for mitochondrial import of enzyme precursor

Extended survival of misfolded G85R SOD1-linked ALS mice by transgenic expression of chaperone Hsp110

Folding in vivo of a newly translated yeast cytosolic enzyme is mediated by the SSA class of cytosolic yeast Hsp70 proteins ⬇️

Folding trajectories of human dihydrofolate reductase inside the GroEL GroES chaperonin cavity and free in solution

Folding with and without encapsulation by cis- and trans-only GroEL-GroES complexes

Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL.

GroEL-GroES-mediated protein folding

GroEL/ES inhibitors as potential antibiotics

GroEL/GroES cycling: ATP binds to an open ring before substrate protein favoring protein binding and production of the native state

Hepadnavirus envelope proteins regulate covalently closed circular DNA amplification

Hsp110 mitigates α-synuclein pathology in vivo

Hydrogen–deuterium exchange in vivo to measure turnover of an ALS‐associated mutant SOD1 protein in spinal cord of mice ⬇️

Import and processing of human ornithine transcarbamoylase precursor by mitochondria from Saccharomyces cerevisiae

Inherited hepatic enzyme defects as candidates for liver-directed gene therapy

Linkage mapping of a mouse gene, iv, that controls left-right asymmetry of the heart and viscera

Localization of GroEL determined by in vivo incorporation of a fluorescent amino acid

Loops in the central channel of ClpA chaperone mediate protein binding, unfolding, and translocation

Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT

Meiotic expression of human ornithine transcarbamylase in the testes of transgenic mice

Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria

Mitochondrial protein import

Molecular chaperone Hsp110 rescues a vesicle transport defect produced by an ALS-associated mutant SOD1 protein in squid axoplasm

Molecular chaperones in biology and medicine at Obernai

Molecular chaperones in cellular protein folding: the birth of a field.

Molecular cloning of the cDNA coding for rat ornithine transcarbamoylase

Multiple states of a nucleotide-bound group 2 chaperonin

NMR analysis of a 900K GroEL GroES complex

Native-like structure of a protein-folding intermediate bound to the chaperonin GroEL.

No evidence for a forced-unfolding mechanism during ATP/GroES binding to substrate-bound GroEL: no observable protection of metastable Rubisco intermediate or GroEL-bound Rubisco from tritium exchange

Nuclear magnetic resonance spectroscopy with the stringent substrate rhodanese bound to the single-ring variant SR1 of the E. coli chaperonin GroEL.

Perturbed ATPase activity and not "close confinement" of substrate in the cis cavity affects rates of folding by tail-multiplied GroEL.

Prevention of protein denaturation under heat stress by the chaperonin Hsp60

Probing the sequence of conformationally induced polarity changes in the molecular chaperonin GroEL with fluorescence spectroscopy

Production of RNA for transcriptomic analysis from mouse spinal cord motor neuron cell bodies by laser capture microdissection

Progressive aggregation despite chaperone associations of a mutant SOD1-YFP in transgenic mice that develop ALS.

Protein aggregation in disease: a role for folding intermediates forming specific multimeric interactions

Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis

Protein folding in the cell: an inside story ⬇️

Protein folding in the cell: functions of two families of molecular chaperone, hsp 60 and TF55-TCP1

Proton-proton Overhauser NMR spectroscopy with polypeptide chains in large structures

QnAs with Arthur L. Horwich. Interview by Prashant Nair

RNA-Seq profiling of spinal cord motor neurons from a presymptomatic SOD1 ALS mouse

Recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 leads to early-onset progressive neurodegeneration

Reduced high-frequency motor neuron firing, EMG fractionation, and gait variability in awake walking ALS mice

Requirement for binding multiple ATPs to convert a GroEL ring to the folding-active state

Residues in chaperonin GroEL required for polypeptide binding and release

Role of the -phosphate of ATP in triggering protein folding by GroEL-GroES: function, structure and energetics

Roles of the N-domains of the ClpA unfoldase in binding substrate proteins and in stable complex formation with the ClpP protease

Selective degeneration of a physiological subtype of spinal motor neuron in mice with SOD1-linked ALS

Solution NMR techniques for large molecular and supramolecular structures

Structure and Expression of a Complementary DNA for the Nuclear Coded Precursor of Human Mitochondrial Ornithine Transcarbamylase ⬇️

Structure and allostery of the chaperonin GroEL.

Substrate polypeptide presents a load on the apical domains of the chaperonin GroEL.

Synthesis of hepadnavirus particles that contain replication-defective duck hepatitis B virus genomes in cultured HuH7 cells