List of works - Hanne Mørck Nielsen

19F-substituted amino acids as an alternative to fluorophore labels: monitoring of degradation and cellular uptake of analogues of penetratin by 19F NMR

scientific article published on 18 March 2019

Animal models for evaluation of oral delivery of biopharmaceuticals

scientific article

Antimicrobial activity of GN peptides and their mode of action

scientific article

Biodegradable nanocomposite microparticles as drug delivering injectable cell scaffolds

scientific article published on 20 July 2011

Biopolymer nanogels improve antibacterial activity and safety profile of a novel lysine-based α-peptide/β-peptoid peptidomimetic

scientific article published on 29 March 2018

Buccal delivery of metformin: TR146 cell culture model evaluating the use of bioadhesive chitosan discs for drug permeability enhancement

scientific article published on 20 October 2013

CAF01 liposomes as a mucosal vaccine adjuvant: In vitro and in vivo investigations

scientific article published on 29 October 2009

Cell-Penetrating Peptides as Carriers for Oral Delivery of Biopharmaceuticals

scientific article

Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections

scientific article

Cellular Effects and Delivery Propensity of Penetratin Is Influenced by Conjugation to Parathyroid Hormone Fragment 1-34 in Synergy with pH.

scientific article published on 20 November 2017

Cellular uptake and membrane-destabilising properties of alpha-peptide/beta-peptoid chimeras: lessons for the design of new cell-penetrating peptides

scientific article

Characterization of particulate drug delivery systems for oral delivery of Peptide and protein drugs

scientific article published on January 2015

Characterization of spray dried bioadhesive metformin microparticles for oromucosal administration

scientific article published on 15 June 2013

Ciprofloxacin-loaded sodium alginate/poly (lactic-co-glycolic acid) electrospun fibrous mats for wound healing

scientific article published on 9 November 2017

Combining diagnostic methods for antimicrobial susceptibility testing - A comparative approach

scientific article published on 14 November 2017

Comparison of polymeric siRNA nanocarriers in a murine LPS-activated macrophage cell line: gene silencing, toxicity and off-target gene expression

scientific article published on 5 October 2011

Conjugation of cell-penetrating peptides to parathyroid hormone affects its structure, potency, and transepithelial permeation

scientific article published on 4 March 2015

Corticosteroid solubility and lipid polarity control release from solid lipid nanoparticles

scientific article published on 25 October 2009

Cyclodextrins: Efficient biocompatible solubilizing excipients for bromhexine liquid and semi-solid drug delivery systems

scientific article published on 17 October 2011

Delivery of siRNA Complexed with Palmitoylated α-Peptide/β-Peptoid Cell-Penetrating Peptidomimetics: Membrane Interaction and Structural Characterization of a Lipid-Based Nanocarrier System

scientific article published on 10 December 2015

Design and characterization of core-shell mPEG-PLGA composite microparticles for development of cell-scaffold constructs

scientific article published in September 2013

Design of an inhalable dry powder formulation of DOTAP-modified PLGA nanoparticles loaded with siRNA.

scientific article

Designing CAF-adjuvanted dry powder vaccines: spray drying preserves the adjuvant activity of CAF01.

scientific article published on 8 February 2013

Elucidating the molecular mechanism of PAMAM-siRNA dendriplex self-assembly: effect of dendrimer charge density

scientific article

End group modification: Efficient tool for improving activity of antimicrobial peptide analogues towards Gram-positive bacteria

scientific article published on 23 January 2015

Engineering of an inhalable DDA/TDB liposomal adjuvant: a quality-by-design approach towards optimization of the spray drying process

scientific article

Engineering of small interfering RNA-loaded lipidoid-poly(DL-Lactic-Co-Glycolic Acid) hybrid nanoparticles for highly efficient and safe gene silencing: A quality by design-based approach

scientific article

Evaluation of carrier-mediated siRNA delivery: lessons for the design of a stem-loop qPCR-based approach for quantification of intracellular full-length siRNA.

scientific article published on 11 January 2013

Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

scientific article published on October 2004

Exploring the mucoadhesive behavior of sucrose acetate isobutyrate: a novel excipient for oral delivery of biopharmaceuticals.

scientific article published in December 2019

Fluorophore labeling of a cell-penetrating peptide induces differential effects on its cellular distribution and affects cell viability

scientific article published on 14 September 2017

Fluorophore labeling of a cell-penetrating peptide significantly alters the mode and degree of biomembrane interaction.

scientific article

High loading efficiency and sustained release of siRNA encapsulated in PLGA nanoparticles: quality by design optimization and characterization

scientific article published on 18 November 2010

Hyaluronic Acid-Based Nanogels Produced by Microfluidics-Facilitated Self-Assembly Improves the Safety Profile of the Cationic Host Defense Peptide Novicidin

scientific article published on 27 March 2015

Immunity by formulation design: induction of high CD8+ T-cell responses by poly(I:C) incorporated into the CAF01 adjuvant via a double emulsion method

scientific article

Immunogenicity Testing of Lipidoids In Vitro and In Silico: Modulating Lipidoid-Mediated TLR4 Activation by Nanoparticle Design.

scientific article

Impact of Lipid-Based Drug Delivery Systems on the Transport and Uptake of Insulin Across Caco-2 Cell Monolayers

Impact of capacity-limited binding on recombinant factor VIII and von Willebrand factor pharmacokinetics in hemophilia A rats

scientific article published on 09 May 2019

In Vitro ADME Properties of Two Novel Antimicrobial Peptoid-Based Compounds as Potential Agents against Canine Pyoderma

scientific article published on 10 March 2018

In vitro cutaneous application of ISCOMs on human skin enhances delivery of hydrophobic model compounds through the stratum corneum

scientific article

In vitro placental model optimization for nanoparticle transport studies

scientific article published on 31 January 2012

Incorporation of a synthetic mycobacterial monomycoloyl glycerol analogue stabilizes dimethyldioctadecylammonium liposomes and potentiates their adjuvant effect in vivo

scientific article

Increased Carrier Peptide Stability through pH Adjustment Improves Insulin and PTH(1-34) Delivery In Vitro and In Vivo Rather than by Enforced Carrier Peptide-Cargo Complexation

scientific article published on 20 October 2020

Inhalable Antimicrobials for Treatment of Bacterial Biofilm-Associated Sinusitis in Cystic Fibrosis Patients: Challenges and Drug Delivery Approaches

scientific article

Interaction of Laponite with Membrane Components-Consequences for Bacterial Aggregation and Infection Confinement

scientific article published on 17 April 2019

Interactions between Surfactants in Solution and Electrospun Protein Fibers: Effects on Release Behavior and Fiber Properties

scientific article published on 21 September 2015

Investigation of protein distribution in solid lipid particles and its impact on protein release using coherent anti-Stokes Raman scattering microscopy

scientific article published on 3 November 2014

Investigation of skin permeation, ex vivo inhibition of venom-induced tissue destruction, and wound healing of African plants used against snakebites

scientific article

Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems

scientific article

Large-Scale Biophysical Evaluation of Protein PEGylation Effects: In Vitro Properties of 61 Protein Entities

scientific article published on 4 April 2016

Lipid Shell-Enveloped Polymeric Nanoparticles with High Integrity of Lipid Shells Improve Mucus Penetration and Interaction with Cystic Fibrosis-Related Bacterial Biofilms.

scientific article published on 23 February 2018

Liposomes for phospholipase A2 triggered siRNA release: preparation and in vitro test

scientific article published on 9 November 2006

Lysine-Based α-Peptide/β-Peptoid Peptidomimetics: Influence of Hydrophobicity, Fluorination, and Distribution of Cationic Charge on Antimicrobial Activity and Cytotoxicity

scientific article published on 4 January 2017

MRI-assessed therapeutic effects of locally administered PLGA nanoparticles loaded with anti-inflammatory siRNA in a murine arthritis model

scientific article published on 11 May 2012

Mechanistic profiling of the siRNA delivery dynamics of lipid-polymer hybrid nanoparticles

scientific article published on 23 December 2014

Metabolic cleavage and translocation efficiency of selected cell penetrating peptides: a comparative study with epithelial cell cultures

scientific article

Metabolic cleavage of cell-penetrating peptides in contact with epithelial models: human calcitonin (hCT)-derived peptides, Tat(47-57) and penetratin(43-58)

scientific article

Mucoadhesive Electrospun Patch Delivery of Lidocaine to the Oral Mucosa and Investigation of Spatial Distribution in a Tissue Using MALDI-Mass Spectrometry Imaging

scientific article published on 15 August 2019

NIR transmission spectroscopy for rapid determination of lipid and lyoprotector content in liposomal vaccine adjuvant system CAF01.

scientific article published on 23 July 2008

Nanoparticle-mediated delivery of the antimicrobial peptide plectasin against Staphylococcus aureus in infected epithelial cells

scientific article

On the temperature dependence of complex formation between chitosan and proteins

scientific article published on 7 June 2011

Penetratin-Mediated Transepithelial Insulin Permeation: Importance of Cationic Residues and pH for Complexation and Permeation

scientific article

Poly(ethylene carbonate)-containing polylactic acid microparticles with rifampicin improve drug delivery to macrophages

scientific article published on 30 May 2018

Preparation and characterization of insulin-surfactant complexes for loading into lipid-based drug delivery systems

scientific article published on 9 July 2013

Preparation and characterization of poly(DL-lactide-co-glycolide) nanoparticles for siRNA delivery

scientific article published on 15 October 2009

Property profiling of biosimilar mucus in a novel mucus-containing in vitro model for assessment of intestinal drug absorption

scientific article

Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection

Self-nanoemulsifying drug delivery systems for oral insulin delivery: in vitro and in vivo evaluations of enteric coating and drug loading

scientific article published on 17 October 2014

Soft hydrogels interpenetrating silicone--A polymer network for drug-releasing medical devices

scientific article published on 17 April 2015

Solid Lipid Particles for Oral Delivery of Peptide and Protein Drugs II – The Digestion of Trilaurin Protects Desmopressin from Proteolytic Degradation

Solid lipid particles for oral delivery of peptide and protein drugs III - the effect of fed state conditions on the in vitro release and degradation of desmopressin

scientific article published on 30 May 2014

Stereochemistry as a determining factor for the effect of a cell-penetrating peptide on cellular viability and epithelial integrity

scientific article published on 24 May 2018

Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation

scientific article published on 23 January 2015

Structure of immune stimulating complex matrices and immune stimulating complexes in suspension determined by small-angle x-ray scattering

scientific article

Studies towards improved cell-penetrating peptide-promoted macromolecular drug delivery

scholarly article by Jacob A.D. Clausen et al published December 2010 in Drug Discovery Today

Surface coating of siRNA-peptidomimetic nano-self-assemblies with anionic lipid bilayers: enhanced gene silencing and reduced adverse effects in vitro

scientific article published on 10 November 2015

Sustained prevention of biofilm formation on a novel silicone matrix suitable for medical devices

scientific article published on 28 May 2015

TR146 cells grown on filters as a model of human buccal epithelium: V. Enzyme activity of the TR146 cell culture model, human buccal epithelium and porcine buccal epithelium, and permeability of leu-enkephalin.

scientific article published on May 2000

Tailoring cytotoxicity of antimicrobial peptidomimetics with high activity against multidrug-resistant Escherichia coli.

scientific article

The impact of particle preparation methods and polymorphic stability of lipid excipients on protein distribution in microparticles

scientific article published on 29 July 2017

The role of mucus as an invisible cloak to transepithelial drug delivery by nanoparticles

scientific article published on 5 November 2017

The surface charge of liposomal adjuvants is decisive for their interactions with the Calu-3 and A549 airway epithelial cell culture models

scientific article published on 13 April 2014

Three-Dimensional Printing of Drug-Eluting Implants: Preparation of an Antimicrobial Polylactide Feedstock Material

Trehalose preserves DDA/TDB liposomes and their adjuvant effect during freeze-drying

scientific article published on 13 May 2007

Ultrasmall TPGS-PLGA Hybrid Nanoparticles for Site-Specific Delivery of Antibiotics into Pseudomonas aeruginosa Biofilms in Lungs

scientific article published on 17 December 2019

alpha,alpha'-trehalose 6,6'-dibehenate in non-phospholipid-based liposomes enables direct interaction with trehalose, offering stability during freeze-drying

scientific article published on 26 January 2008

List of works by Hanne Mørck Nielsen

19F-substituted amino acids as an alternative to fluorophore labels: monitoring of degradation and cellular uptake of analogues of penetratin by 19F NMR

Animal models for evaluation of oral delivery of biopharmaceuticals

Antimicrobial activity of GN peptides and their mode of action

Biodegradable nanocomposite microparticles as drug delivering injectable cell scaffolds

Biopolymer nanogels improve antibacterial activity and safety profile of a novel lysine-based α-peptide/β-peptoid peptidomimetic

Buccal delivery of metformin: TR146 cell culture model evaluating the use of bioadhesive chitosan discs for drug permeability enhancement

CAF01 liposomes as a mucosal vaccine adjuvant: In vitro and in vivo investigations

Cell-Penetrating Peptides as Carriers for Oral Delivery of Biopharmaceuticals

Cell-penetrating antimicrobial peptides - prospectives for targeting intracellular infections

Cellular Effects and Delivery Propensity of Penetratin Is Influenced by Conjugation to Parathyroid Hormone Fragment 1-34 in Synergy with pH.

Cellular uptake and membrane-destabilising properties of alpha-peptide/beta-peptoid chimeras: lessons for the design of new cell-penetrating peptides

Characterization of particulate drug delivery systems for oral delivery of Peptide and protein drugs

Characterization of spray dried bioadhesive metformin microparticles for oromucosal administration

Ciprofloxacin-loaded sodium alginate/poly (lactic-co-glycolic acid) electrospun fibrous mats for wound healing

Combining diagnostic methods for antimicrobial susceptibility testing - A comparative approach

Comparison of polymeric siRNA nanocarriers in a murine LPS-activated macrophage cell line: gene silencing, toxicity and off-target gene expression

Conjugation of cell-penetrating peptides to parathyroid hormone affects its structure, potency, and transepithelial permeation

Corticosteroid solubility and lipid polarity control release from solid lipid nanoparticles

Cyclodextrins: Efficient biocompatible solubilizing excipients for bromhexine liquid and semi-solid drug delivery systems

Delivery of siRNA Complexed with Palmitoylated α-Peptide/β-Peptoid Cell-Penetrating Peptidomimetics: Membrane Interaction and Structural Characterization of a Lipid-Based Nanocarrier System

Design and characterization of core-shell mPEG-PLGA composite microparticles for development of cell-scaffold constructs

Design of an inhalable dry powder formulation of DOTAP-modified PLGA nanoparticles loaded with siRNA.

Designing CAF-adjuvanted dry powder vaccines: spray drying preserves the adjuvant activity of CAF01.

Elucidating the molecular mechanism of PAMAM-siRNA dendriplex self-assembly: effect of dendrimer charge density

End group modification: Efficient tool for improving activity of antimicrobial peptide analogues towards Gram-positive bacteria

Engineering of an inhalable DDA/TDB liposomal adjuvant: a quality-by-design approach towards optimization of the spray drying process

Engineering of small interfering RNA-loaded lipidoid-poly(DL-Lactic-Co-Glycolic Acid) hybrid nanoparticles for highly efficient and safe gene silencing: A quality by design-based approach

Evaluation of carrier-mediated siRNA delivery: lessons for the design of a stem-loop qPCR-based approach for quantification of intracellular full-length siRNA.

Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

Exploring the mucoadhesive behavior of sucrose acetate isobutyrate: a novel excipient for oral delivery of biopharmaceuticals.

Fluorophore labeling of a cell-penetrating peptide induces differential effects on its cellular distribution and affects cell viability

Fluorophore labeling of a cell-penetrating peptide significantly alters the mode and degree of biomembrane interaction.

High loading efficiency and sustained release of siRNA encapsulated in PLGA nanoparticles: quality by design optimization and characterization

Hyaluronic Acid-Based Nanogels Produced by Microfluidics-Facilitated Self-Assembly Improves the Safety Profile of the Cationic Host Defense Peptide Novicidin

Immunity by formulation design: induction of high CD8+ T-cell responses by poly(I:C) incorporated into the CAF01 adjuvant via a double emulsion method

Immunogenicity Testing of Lipidoids In Vitro and In Silico: Modulating Lipidoid-Mediated TLR4 Activation by Nanoparticle Design.

Impact of Lipid-Based Drug Delivery Systems on the Transport and Uptake of Insulin Across Caco-2 Cell Monolayers

Impact of capacity-limited binding on recombinant factor VIII and von Willebrand factor pharmacokinetics in hemophilia A rats

In Vitro ADME Properties of Two Novel Antimicrobial Peptoid-Based Compounds as Potential Agents against Canine Pyoderma

In vitro cutaneous application of ISCOMs on human skin enhances delivery of hydrophobic model compounds through the stratum corneum

In vitro placental model optimization for nanoparticle transport studies

Incorporation of a synthetic mycobacterial monomycoloyl glycerol analogue stabilizes dimethyldioctadecylammonium liposomes and potentiates their adjuvant effect in vivo

Increased Carrier Peptide Stability through pH Adjustment Improves Insulin and PTH(1-34) Delivery In Vitro and In Vivo Rather than by Enforced Carrier Peptide-Cargo Complexation

Inhalable Antimicrobials for Treatment of Bacterial Biofilm-Associated Sinusitis in Cystic Fibrosis Patients: Challenges and Drug Delivery Approaches

Interaction of Laponite with Membrane Components-Consequences for Bacterial Aggregation and Infection Confinement

Interactions between Surfactants in Solution and Electrospun Protein Fibers: Effects on Release Behavior and Fiber Properties

Investigation of protein distribution in solid lipid particles and its impact on protein release using coherent anti-Stokes Raman scattering microscopy

Investigation of skin permeation, ex vivo inhibition of venom-induced tissue destruction, and wound healing of African plants used against snakebites

Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems

Large-Scale Biophysical Evaluation of Protein PEGylation Effects: In Vitro Properties of 61 Protein Entities

Lipid Shell-Enveloped Polymeric Nanoparticles with High Integrity of Lipid Shells Improve Mucus Penetration and Interaction with Cystic Fibrosis-Related Bacterial Biofilms.

Liposomes for phospholipase A2 triggered siRNA release: preparation and in vitro test

Lysine-Based α-Peptide/β-Peptoid Peptidomimetics: Influence of Hydrophobicity, Fluorination, and Distribution of Cationic Charge on Antimicrobial Activity and Cytotoxicity

MRI-assessed therapeutic effects of locally administered PLGA nanoparticles loaded with anti-inflammatory siRNA in a murine arthritis model

Mechanistic profiling of the siRNA delivery dynamics of lipid-polymer hybrid nanoparticles

Metabolic cleavage and translocation efficiency of selected cell penetrating peptides: a comparative study with epithelial cell cultures

Metabolic cleavage of cell-penetrating peptides in contact with epithelial models: human calcitonin (hCT)-derived peptides, Tat(47-57) and penetratin(43-58)

Mucoadhesive Electrospun Patch Delivery of Lidocaine to the Oral Mucosa and Investigation of Spatial Distribution in a Tissue Using MALDI-Mass Spectrometry Imaging

NIR transmission spectroscopy for rapid determination of lipid and lyoprotector content in liposomal vaccine adjuvant system CAF01.

Nanoparticle-mediated delivery of the antimicrobial peptide plectasin against Staphylococcus aureus in infected epithelial cells

On the temperature dependence of complex formation between chitosan and proteins

Penetratin-Mediated Transepithelial Insulin Permeation: Importance of Cationic Residues and pH for Complexation and Permeation

Poly(ethylene carbonate)-containing polylactic acid microparticles with rifampicin improve drug delivery to macrophages

Preparation and characterization of insulin-surfactant complexes for loading into lipid-based drug delivery systems

Preparation and characterization of poly(DL-lactide-co-glycolide) nanoparticles for siRNA delivery

Property profiling of biosimilar mucus in a novel mucus-containing in vitro model for assessment of intestinal drug absorption

Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection

Self-nanoemulsifying drug delivery systems for oral insulin delivery: in vitro and in vivo evaluations of enteric coating and drug loading

Soft hydrogels interpenetrating silicone--A polymer network for drug-releasing medical devices

Solid Lipid Particles for Oral Delivery of Peptide and Protein Drugs II – The Digestion of Trilaurin Protects Desmopressin from Proteolytic Degradation

Solid lipid particles for oral delivery of peptide and protein drugs III - the effect of fed state conditions on the in vitro release and degradation of desmopressin

Stereochemistry as a determining factor for the effect of a cell-penetrating peptide on cellular viability and epithelial integrity

Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation

Structure of immune stimulating complex matrices and immune stimulating complexes in suspension determined by small-angle x-ray scattering

Studies towards improved cell-penetrating peptide-promoted macromolecular drug delivery

Surface coating of siRNA-peptidomimetic nano-self-assemblies with anionic lipid bilayers: enhanced gene silencing and reduced adverse effects in vitro

Sustained prevention of biofilm formation on a novel silicone matrix suitable for medical devices

TR146 cells grown on filters as a model of human buccal epithelium: V. Enzyme activity of the TR146 cell culture model, human buccal epithelium and porcine buccal epithelium, and permeability of leu-enkephalin.

Tailoring cytotoxicity of antimicrobial peptidomimetics with high activity against multidrug-resistant Escherichia coli.