List of works - Sriram Neelamegham

A computational framework for the automated construction of glycosylation reaction networks

scientific article (publication date: 2014)

A systematic analysis of acceptor specificity and reaction kinetics of five human α(2,3)sialyltransferases: Product inhibition studies illustrate reaction mechanism for ST3Gal-I.

scientific article published on 12 December 2015

A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis

scientific article published on 22 July 2021

Affinity and kinetics of sialyl Lewis-X and core-2 based oligosaccharides binding to L- and P-selectin.

scientific article

An analysis tool to quantify the efficiency of cell tethering and firm-adhesion in the parallel-plate flow chamber

scientific article published in July 2003

Analysis of the specificity of sialyltransferases toward mucin core 2, globo, and related structures. identification of the sialylation sequence and the effects of sulfate, fucose, methyl, and fluoro substituents of the carbohydrate chain in the bio

scientific article published on November 2005

Application of fluorescence spectroscopy to quantify shear-induced protein conformation change.

scientific article

Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-association of von Willebrand factor in suspension

scientific article published on 27 November 2002

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model.

scientific article

Characterization of cancer associated mucin type O-glycans using the exchange sialylation properties of mammalian sialyltransferase ST3Gal-II.

scientific article published on 29 February 2012

Competition between core-2 GlcNAc-transferase and ST6GalNAc-transferase regulates the synthesis of the leukocyte selectin ligand on human P-selectin glycoprotein ligand-1

scientific article published on 2 April 2013

Cooperativity between selectins and beta2-integrins define neutrophil capture and stable adhesion in shear flow.

scientific article

Detection of Plasma Protease Activity Using Microsphere-Cytometry Assays with E. coli Derived Substrates: VWF Proteolysis by ADAMTS13

scientific article published on 18 May 2015

Detection of site-specific glycosylation in proteins using flow cytometry

scientific article

Differential regulation of neutrophil CD18 integrin function by di- and tri-valent cations: manganese vs. gadolinium

scientific article published on 4 March 2008

Disulfide trapping of protein complexes on the yeast surface.

scientific article published in May 2010

Escherichia coli-derived von Willebrand factor-A2 domain fluorescence/Förster resonance energy transfer proteins that quantify ADAMTS13 activity

scientific article

Fluid shear induces conformation change in human blood protein von Willebrand factor in solution.

scientific article published on March 2009

Functionalization of cobalt porphyrin-phospholipid bilayers with his-tagged ligands and antigens.

scientific article published on 20 April 2015

Glycosphingolipids on Human Myeloid Cells Stabilize E-Selectin-Dependent Rolling in the Multistep Leukocyte Adhesion Cascade

scientific article published on 11 February 2016

Glycosylation Network Analysis Toolbox: a MATLAB-based environment for systems glycobiology.

scientific article published on 10 December 2012

Identification of Physiologically Relevant Substrates for Cloned Gal: 3-O-Sulfotransferases (Gal3STs)

scientific article published in Journal of Biological Chemistry

Immune complexes formed following the binding of anti-platelet factor 4 (CXCL4) antibodies to CXCL4 stimulate human neutrophil activation and cell adhesion.

scientific article

In silico Biochemical Reaction Network Analysis (IBRENA): a package for simulation and analysis of reaction networks.

scientific article published on 28 February 2008

Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan elaboration

scientific article published on 26 October 2020

Integration of systems glycobiology with bioinformatics toolboxes, glycoinformatics resources, and glycoproteomics data

scientific article

Lipoxin A4 inhibits immune cell binding to salivary epithelium and vascular endothelium.

scientific article

Mammalian sialyltransferase ST3Gal-II: its exchange sialylation catalytic properties allow labeling of sialyl residues in mucin-type sialylated glycoproteins and specific gangliosides

scientific article published on 13 October 2011

Overexpression of α2,3sialyl T-antigen in breast cancer determined by miniaturized glycosyltransferase assays and confirmed using tissue microarray immunohistochemical analysis

scientific article

PPLATE: a computer program for analysis of parallel-plate flow chamber experimental data.

scientific article

Peracetylated 4-fluoro-glucosamine reduces the content and repertoire of N- and O-glycans without direct incorporation.

scientific article

Platelet GpIba binding to von Willebrand Factor under fluid shear:contributions of the D′D3-domain, A1-domain flanking peptide and O-linked glycans

scientific article

Reversible sialylation: synthesis of cytidine 5'-monophospho-N-acetylneuraminic acid from cytidine 5'-monophosphate with alpha2,3-sialyl O-glycan-, glycolipid-, and macromolecule-based donors yields diverse sialylated products.

scientific article published on 8 December 2007

Robustness in glycosylation systems: effect of modified monosaccharides, acceptor decoys and azido sugars on cellular nucleotide-sugar levels and pattern of N-linked glycosylation

scientific article published on 30 April 2020

ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes.

scientific article

Scaling down the size and increasing the throughput of glycosyltransferase assays: activity changes on stem cell differentiation

scientific article

Sensitivity, principal component and flux analysis applied to signal transduction: the case of epidermal growth factor mediated signaling

scientific article

Silencing α1,3-fucosyltransferases in human leukocytes reveals a role for FUT9 enzyme during E-selectin-mediated cell adhesion.

scientific article published on 28 November 2012

Solution structure of human von Willebrand factor studied using small angle neutron scattering.

scientific article published on 19 October 2006

Sulfonated Polyethylenimine for Photosensitizer Conjugation and Targeting

scientific article

Systems glycobiology: biochemical reaction networks regulating glycan structure and function.

scientific article published on 24 March 2011

Systems-level modeling of cellular glycosylation reaction networks: O-linked glycan formation on natural selectin ligands.

scientific article published on 07 October 2008

Systems-level studies of glycosyltransferase gene expression and enzyme activity that are associated with the selectin binding function of human leukocytes.

scientific article published on 20 August 2008

The computing platelet: integrating environmental cues ⬇️

scientific article published on 01 July 2012

The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling

scientific article

Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion.

scientific article published in January 2004

Understanding glycomechanics using mathematical modeling: a review of current approaches to simulate cellular glycosylation reaction networks.

scientific article

von Willebrand factor (VWF) propeptide binding to VWF D'D3 domain attenuates platelet activation and adhesion

scientific article

von Willebrand factor self-association on platelet GpIbalpha under hydrodynamic shear: effect on shear-induced platelet activation

scientific article

List of works by Sriram Neelamegham

A computational framework for the automated construction of glycosylation reaction networks

A systematic analysis of acceptor specificity and reaction kinetics of five human α(2,3)sialyltransferases: Product inhibition studies illustrate reaction mechanism for ST3Gal-I.

A systems-based framework to computationally describe putative transcription factors and signaling pathways regulating glycan biosynthesis

Affinity and kinetics of sialyl Lewis-X and core-2 based oligosaccharides binding to L- and P-selectin.

An analysis tool to quantify the efficiency of cell tethering and firm-adhesion in the parallel-plate flow chamber

Analysis of the specificity of sialyltransferases toward mucin core 2, globo, and related structures. identification of the sialylation sequence and the effects of sulfate, fucose, methyl, and fluoro substituents of the carbohydrate chain in the bio

Application of fluorescence spectroscopy to quantify shear-induced protein conformation change.

Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-association of von Willebrand factor in suspension

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model.

Characterization of cancer associated mucin type O-glycans using the exchange sialylation properties of mammalian sialyltransferase ST3Gal-II.

Competition between core-2 GlcNAc-transferase and ST6GalNAc-transferase regulates the synthesis of the leukocyte selectin ligand on human P-selectin glycoprotein ligand-1

Cooperativity between selectins and beta2-integrins define neutrophil capture and stable adhesion in shear flow.

Detection of Plasma Protease Activity Using Microsphere-Cytometry Assays with E. coli Derived Substrates: VWF Proteolysis by ADAMTS13

Detection of site-specific glycosylation in proteins using flow cytometry

Differential regulation of neutrophil CD18 integrin function by di- and tri-valent cations: manganese vs. gadolinium

Disulfide trapping of protein complexes on the yeast surface.

Escherichia coli-derived von Willebrand factor-A2 domain fluorescence/Förster resonance energy transfer proteins that quantify ADAMTS13 activity

Fluid shear induces conformation change in human blood protein von Willebrand factor in solution.

Functionalization of cobalt porphyrin-phospholipid bilayers with his-tagged ligands and antigens.

Glycosphingolipids on Human Myeloid Cells Stabilize E-Selectin-Dependent Rolling in the Multistep Leukocyte Adhesion Cascade

Glycosylation Network Analysis Toolbox: a MATLAB-based environment for systems glycobiology.

Identification of Physiologically Relevant Substrates for Cloned Gal: 3-O-Sulfotransferases (Gal3STs)

Immune complexes formed following the binding of anti-platelet factor 4 (CXCL4) antibodies to CXCL4 stimulate human neutrophil activation and cell adhesion.

In silico Biochemical Reaction Network Analysis (IBRENA): a package for simulation and analysis of reaction networks.

Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan elaboration

Integration of systems glycobiology with bioinformatics toolboxes, glycoinformatics resources, and glycoproteomics data

Lipoxin A4 inhibits immune cell binding to salivary epithelium and vascular endothelium.

Mammalian sialyltransferase ST3Gal-II: its exchange sialylation catalytic properties allow labeling of sialyl residues in mucin-type sialylated glycoproteins and specific gangliosides

Overexpression of α2,3sialyl T-antigen in breast cancer determined by miniaturized glycosyltransferase assays and confirmed using tissue microarray immunohistochemical analysis

PPLATE: a computer program for analysis of parallel-plate flow chamber experimental data.

Peracetylated 4-fluoro-glucosamine reduces the content and repertoire of N- and O-glycans without direct incorporation.

Platelet GpIba binding to von Willebrand Factor under fluid shear:contributions of the D′D3-domain, A1-domain flanking peptide and O-linked glycans

Reversible sialylation: synthesis of cytidine 5'-monophospho-N-acetylneuraminic acid from cytidine 5'-monophosphate with alpha2,3-sialyl O-glycan-, glycolipid-, and macromolecule-based donors yields diverse sialylated products.

Robustness in glycosylation systems: effect of modified monosaccharides, acceptor decoys and azido sugars on cellular nucleotide-sugar levels and pattern of N-linked glycosylation

ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes.

Scaling down the size and increasing the throughput of glycosyltransferase assays: activity changes on stem cell differentiation

Sensitivity, principal component and flux analysis applied to signal transduction: the case of epidermal growth factor mediated signaling

Silencing α1,3-fucosyltransferases in human leukocytes reveals a role for FUT9 enzyme during E-selectin-mediated cell adhesion.

Solution structure of human von Willebrand factor studied using small angle neutron scattering.

Sulfonated Polyethylenimine for Photosensitizer Conjugation and Targeting

Systems glycobiology: biochemical reaction networks regulating glycan structure and function.

Systems-level modeling of cellular glycosylation reaction networks: O-linked glycan formation on natural selectin ligands.

Systems-level studies of glycosyltransferase gene expression and enzyme activity that are associated with the selectin binding function of human leukocytes.

The computing platelet: integrating environmental cues ⬇️

The use of surface immobilization of P-selectin glycoprotein ligand-1 on mesenchymal stem cells to facilitate selectin mediated cell tethering and rolling

Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion.

Understanding glycomechanics using mathematical modeling: a review of current approaches to simulate cellular glycosylation reaction networks.

von Willebrand factor (VWF) propeptide binding to VWF D'D3 domain attenuates platelet activation and adhesion

von Willebrand factor self-association on platelet GpIbalpha under hydrodynamic shear: effect on shear-induced platelet activation