List of works - Josef Chmelík

1H, 13C and 15N resonance assignments of human DCL-1 (CD302) extracellular domain.

scientific article published on 2 January 2016

A new approach in proteomics of wheat gluten: combining chymotrypsin cleavage and matrix-assisted laser desorption/ionization quadrupole ion trap reflectron tandem mass spectrometry.

scientific article published in January 2005

A proteomic approach to studying the differentiation of neural stem cells

scientific article

Applications of field-flow fractionation in proteomics: presence and future

scientific article published on August 2007

Bioaffinity magnetic reactor for peptide digestion followed by analysis using bottom-up shotgun proteomics strategy.

scientific article

Chemical Cross-Linking and H/D Exchange for Fast Refinement of Protein Crystal Structure

scientific article published on 23 December 2011

Complex analytical approach to characterization of the influence of carbon dioxide concentration on carbohydrate composition in Norway spruce needles

scientific article

Detection of hydrolysis of lipid post-translational modifications during gel-electrophoresis-based proteomic protocol

scientific article

Determination of carbohydrates in juices by capillary electrophoresis, high-performance liquid chromatography, and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

scientific article published in February 2004

Different behavior of dextrans in positive-ion and negative-ion mass spectrometry

scientific article

Different elution modes and field programming in gravitational field-flow fractionation. Effect of channel angle

scientific article published on 10 September 2008

Different elution modes and field programming in gravitational field-flow fractionation. IV. Field programming achieved with channels of non-constant cross-sections

scientific article published on 01 April 2002

Different elution modes and field programming in gravitational field-flow fractionation: field programming using density and viscosity gradients

scientific article published on 11 May 2006

Electrospray ionization ion-trap multistage mass spectrometric study of sodium cationized aldobiuronic and pseudoaldobiuronic acid derivatives

scientific article published in December 2004

High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli.

scientific article published in February 2011

Human Stress-inducible Hsp70 Has a High Propensity to Form ATP-dependent Antiparallel Dimers That Are Differentially Regulated by Cochaperone Binding

scientific article published on 20 November 2018

Identification of N-glycosylation in prolyl endoprotease from Aspergillus niger and evaluation of the enzyme for its possible application in proteomics.

scientific article published in November 2009

Identification of proteins by combination of size-exclusion chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and comparison of some desalting procedures for both intact proteins and their tryptic diges

scientific article

Impact of Chemical Cross-Linking on Protein Structure and Function.

scientific article published on 12 December 2017

Improvement of quality of peptide mass spectra in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and post-source decay analysis of salty protein digests by using on-target washing

scientific article published on 01 December 2003

Influence of different proteomic protocols on degree of high-coverage identification of nonspecific lipid transfer protein 1 modified during malting

scientific article published on 01 February 2009

Inhibition of plant amine oxidases by a novel series of diamine derivatives

scientific article

Internal consistency of NMR data obtained in partially aligned biomacromolecules

scientific article

Investigation of thermal denaturation of barley nonspecific lipid transfer protein 1 (ns-LTP1b) by nuclear magnetic resonance and differential scanning calorimetry.

scientific article published in September 2009

MS-Based Approaches Enable the Structural Characterization of Transcription Factor/DNA Response Element Complex

scientific article published on 26 September 2019

Mass spectrometric characterization of low-molecular-mass color pI markers and their use for direct determination of pI value of proteins

scientific article

Motif orientation matters: structural characterization of TEAD1 recognition of genomic DNA

scientific article published on 10 December 2020

NMR structure of the N-terminal domain of capsid protein from the mason-pfizer monkey virus

scientific article

Negative charge of the AC-to-Hly linking segment modulates calcium-dependent membrane activities of Bordetella adenylate cyclase toxin

scientific article published on 22 April 2020

Negative electrospray, ion trap multistage mass spectrometry of synthetic fragments of the O-PS of Vibrio cholerae O:1.

scientific article published in January 2006

Negative matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry fragmentation of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O:1 in the presence of anionic dopants

scientific article

Novel staining-free proteomic method for simultaneous identification of proteins and determination of their pI values by using low-molecular-mass pI markers

scientific article published on 01 September 2007

Off-line combination of reversed-phase liquid chromatography and laser desorption/ionization time-of-flight mass spectrometry with seamless post-source decay fragment ion analysis for characterization of square-planar nickel(II) complexes

scientific article

Positive-ion fragmentation in matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O:1.

scientific article

Preparation of soluble isotopically labeled NKp30, a human natural cytotoxicity receptor, for structural studies using NMR

scientific article published on 08 October 2012

Probing the Ca(2+)-assisted π-π interaction during Ca(2+)-dependent protein folding.

scientific article published on 22 October 2015

Proteomic identification of technologically modified proteins in malt by combination of protein fractionation using convective interaction media and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

scientific article

Relative quantitation of proteins fractionated by the ProteomeLab PF 2D system using isobaric tags for relative and absolute quantitation (iTRAQ).

scientific article published on 28 August 2007

Sample deposition device for off-line combination of supercritical fluid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

scientific article

Simple and fast method for recognition of reducing and nonreducing neutral carbohydrates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

scientific article

Size characterization of barley starch granules by gravitational field-flow fractionation: a rapid, low-cost method to assess the brewing capability of different strains

scientific article published in April 2002

Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO(2) concentration

scientific article published in October 2008

Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice

scientific article

Solution structure of the lymphocyte receptor Nkrp1a reveals a distinct conformation of the long loop region as compared to in the crystal structure

scientific article

Spectral density mapping at multiple magnetic fields suitable for (13)C NMR relaxation studies.

scientific article

Structure of Bombyx mori chemosensory protein 1 in solution

scientific article

The C-type lectin-like receptor Nkrp1b: Structural proteomics reveals features affecting protein conformation and interactions

scientific article published on 08 November 2018

The combination of hydrogen/deuterium exchange or chemical cross-linking techniques with mass spectrometry: mapping of human 14-3-3ζ homodimer interface

scientific article published on 2 May 2012

The influence of nonspecific cleavage sites on identification of low molecular mass proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with seamless post-source decay fragment ion analysis

scientific article published on 01 January 2005

List of works by Josef Chmelík

1H, 13C and 15N resonance assignments of human DCL-1 (CD302) extracellular domain.

A new approach in proteomics of wheat gluten: combining chymotrypsin cleavage and matrix-assisted laser desorption/ionization quadrupole ion trap reflectron tandem mass spectrometry.

A proteomic approach to studying the differentiation of neural stem cells

Applications of field-flow fractionation in proteomics: presence and future

Bioaffinity magnetic reactor for peptide digestion followed by analysis using bottom-up shotgun proteomics strategy.

Chemical Cross-Linking and H/D Exchange for Fast Refinement of Protein Crystal Structure

Complex analytical approach to characterization of the influence of carbon dioxide concentration on carbohydrate composition in Norway spruce needles

Detection of hydrolysis of lipid post-translational modifications during gel-electrophoresis-based proteomic protocol

Determination of carbohydrates in juices by capillary electrophoresis, high-performance liquid chromatography, and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

Different behavior of dextrans in positive-ion and negative-ion mass spectrometry

Different elution modes and field programming in gravitational field-flow fractionation. Effect of channel angle

Different elution modes and field programming in gravitational field-flow fractionation. IV. Field programming achieved with channels of non-constant cross-sections

Different elution modes and field programming in gravitational field-flow fractionation: field programming using density and viscosity gradients

Electrospray ionization ion-trap multistage mass spectrometric study of sodium cationized aldobiuronic and pseudoaldobiuronic acid derivatives

High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli.

Human Stress-inducible Hsp70 Has a High Propensity to Form ATP-dependent Antiparallel Dimers That Are Differentially Regulated by Cochaperone Binding

Identification of N-glycosylation in prolyl endoprotease from Aspergillus niger and evaluation of the enzyme for its possible application in proteomics.

Identification of proteins by combination of size-exclusion chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and comparison of some desalting procedures for both intact proteins and their tryptic diges

Impact of Chemical Cross-Linking on Protein Structure and Function.

Improvement of quality of peptide mass spectra in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and post-source decay analysis of salty protein digests by using on-target washing

Influence of different proteomic protocols on degree of high-coverage identification of nonspecific lipid transfer protein 1 modified during malting

Inhibition of plant amine oxidases by a novel series of diamine derivatives

Internal consistency of NMR data obtained in partially aligned biomacromolecules

Investigation of thermal denaturation of barley nonspecific lipid transfer protein 1 (ns-LTP1b) by nuclear magnetic resonance and differential scanning calorimetry.

MS-Based Approaches Enable the Structural Characterization of Transcription Factor/DNA Response Element Complex

Mass spectrometric characterization of low-molecular-mass color pI markers and their use for direct determination of pI value of proteins

Motif orientation matters: structural characterization of TEAD1 recognition of genomic DNA

NMR structure of the N-terminal domain of capsid protein from the mason-pfizer monkey virus

Negative charge of the AC-to-Hly linking segment modulates calcium-dependent membrane activities of Bordetella adenylate cyclase toxin

Negative electrospray, ion trap multistage mass spectrometry of synthetic fragments of the O-PS of Vibrio cholerae O:1.

Negative matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry fragmentation of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O:1 in the presence of anionic dopants

Novel staining-free proteomic method for simultaneous identification of proteins and determination of their pI values by using low-molecular-mass pI markers

Off-line combination of reversed-phase liquid chromatography and laser desorption/ionization time-of-flight mass spectrometry with seamless post-source decay fragment ion analysis for characterization of square-planar nickel(II) complexes

Positive-ion fragmentation in matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O:1.

Preparation of soluble isotopically labeled NKp30, a human natural cytotoxicity receptor, for structural studies using NMR

Probing the Ca(2+)-assisted π-π interaction during Ca(2+)-dependent protein folding.

Proteomic identification of technologically modified proteins in malt by combination of protein fractionation using convective interaction media and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Relative quantitation of proteins fractionated by the ProteomeLab PF 2D system using isobaric tags for relative and absolute quantitation (iTRAQ).

Sample deposition device for off-line combination of supercritical fluid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Simple and fast method for recognition of reducing and nonreducing neutral carbohydrates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Size characterization of barley starch granules by gravitational field-flow fractionation: a rapid, low-cost method to assess the brewing capability of different strains

Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO(2) concentration

Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice

Solution structure of the lymphocyte receptor Nkrp1a reveals a distinct conformation of the long loop region as compared to in the crystal structure

Spectral density mapping at multiple magnetic fields suitable for (13)C NMR relaxation studies.

Structure of Bombyx mori chemosensory protein 1 in solution

The C-type lectin-like receptor Nkrp1b: Structural proteomics reveals features affecting protein conformation and interactions

The combination of hydrogen/deuterium exchange or chemical cross-linking techniques with mass spectrometry: mapping of human 14-3-3ζ homodimer interface

The influence of nonspecific cleavage sites on identification of low molecular mass proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with seamless post-source decay fragment ion analysis