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List of works by Tim Dafforn

6-mer peptide selectively anneals to a pathogenic serpin conformation and blocks polymerization. Implications for the prevention of Z alpha(1)-antitrypsin-related cirrhosis

scientific article published on 28 December 2001

A Kinetic Mechanism for the Polymerization of α1-Antitrypsin

scientific article published in Journal of Biological Chemistry

A cluster of familial Creutzfeldt-Jakob disease mutations recapitulate conserved residues in Doppel: a case of molecular mimicry?

scientific article published in December 2002

A conformational change in TFIIB is required for activator-mediated assembly of the preinitiation complex.

scientific article

A general method for relieving substrate inhibition in lactate dehydrogenases

scientific article published on 01 June 1999

A method for detergent-free isolation of membrane proteins in their local lipid environment. ⬇️

scientific article

A novel model and molecular therapy for Z alpha-1 antitrypsin deficiency.

scientific article

A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers

scientific article published on 01 December 2018

A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain

scientific article

An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles.

scientific article published in June 2018

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

scientific article published in Scientific Reports

Antithrombins Wibble and Wobble (T85M/K): archetypal conformational diseases with in vivo latent-transition, thrombosis, and heparin activation.

scientific article

Assembly pathway of a designed alpha-helical protein fiber.

scientific article

Automated High-Throughput Capillary Circular Dichroism and Intrinsic Fluorescence Spectroscopy for Rapid Determination of Protein Structure

scientific article published on 17 October 2019

Breaking the 200 nm limit for routine flow linear dichroism measurements using UV synchrotron radiation.

scientific article published on 19 September 2008

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum.

scientific article

Characterization of the necrotic protein that regulates the Toll-mediated immune response in Drosophila

scientific article published on 31 October 2002

Characterizing the assembly of the Sup35 yeast prion fragment, GNNQQNY: structural changes accompany a fiber-to-crystal switch.

scientific article published on January 2010

Correlation of the enzyme activities of Bacillus stearothermophilus lactate dehydrogenase on three substrates with the results of molecular dynamics/energy minimization conformational searching

scientific article published on 01 October 1997

DNA compaction by the higher-order assembly of PRH/Hex homeodomain protein oligomers.

scientific article published on 31 July 2010

De novo design of Ln(III) coiled coils for imaging applications.

scientific article

Detection of pathogenic bacteria using a homogeneous immunoassay based on shear alignment of virus particles and linear dichroism.

scientific article published on 12 December 2011

Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: the power of native nanodiscs

scientific article

Detergent-free purification of ABC (ATP-binding-cassette) transporters.

scientific article published on July 2014

Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle

scientific article

Drosophila necrotic mutations mirror disease-associated variants of human serpins

scientific article published in April 2003

Dye Aggregate-Mediated Self-Assembly of Bacteriophage Bioconjugates

scientific article published on 31 October 2018

Encapsulated membrane proteins: A simplified system for molecular simulation.

scientific article published on 2 March 2016

Expression, purification and crystallization of the cell-division protein YgfE from Escherichia coli.

scientific article

Flow linear dichroism of some prototypical proteins.

scientific article published in September 2009

Fluorescence detected linear dichroism spectroscopy: A selective and sensitive probe for fluorophores in flow-oriented systems.

scientific article published on 3 January 2018

Folding and Membrane Insertion of the Pore-Forming Peptide Gramicidin Occur as a Concerted Process

scientific article published on 07 August 2008

FtsZ fiber bundling is triggered by a conformational change in bound GTP.

scientific article

FtsZ polymer-bundling by the Escherichia coli ZapA orthologue, YgfE, involves a conformational change in bound GTP.

scientific article published on 19 March 2007

Functional and biophysical analysis of the C-terminus of the CGRP-receptor; a family B GPCR.

scientific article published on 18 July 2008

Functional domains of the human epididymal protease inhibitor, eppin

scientific article

GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential.

scientific article published on April 2016

Golgi protein FAPP2 tubulates membranes

scientific article

Heteropolymerization of S, I, and Z alpha1-antitrypsin and liver cirrhosis.

scientific article

High-resolution, three-dimensional modeling of human leukocyte antigen class I structure and surface electrostatic potential reveals the molecular basis for alloantibody binding epitopes

scientific article published on 28 July 2011

Identification and characterization of trimethylamine N-oxide (TMAO) demethylase and TMAO permease in Methylocella silvestris BL2.

scientific article

Identification of a 4-mer Peptide Inhibitor that Effectively Blocks the Polymerization of Pathogenic Z α1-Antitrypsin

scientific article published on 15 June 2006

Identification of the primary collagen-binding surface on human glycoprotein VI by site-directed mutagenesis and by a blocking phage antibody

scientific article published on 22 September 2003

Inhibiting polymerization: new therapeutic strategies for Z alpha1-antitrypsin-related emphysema

scientific article published on 11 March 2004

Inhibitory activity of the Drosophila melanogaster serpin Necrotic is dependent on lysine residues in the D-helix

scientific article published on 11 July 2006

Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability

scientific article published in September 2003

Kinetic study of the thermal denaturation of a hyperthermostable extracellular α-amylase from Pyrococcus furiosus.

scientific article published on 21 September 2013

Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

scientific article published on 01 May 2019

Linear dichroism for the detection of single base pair mutationsElectronic supplementary information (ESI) available: Figures S1–S4. See http://www.rsc.org/suppdata/cc/b1/b107830p/

scientific article published on 01 December 2001

Linear dichroism of biomolecules: which way is up?

scientific article published in October 2004

Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold

scientific article published on 20 August 2018

Location of auxilin within a clathrin cage.

scientific article published on February 2004

Looking at long molecules in solution: what happens when they are subjected to Couette flow?

scientific article published on 14 June 2006

Membrane protein extraction and purification using styrene-maleic acid (SMA) copolymer: effect of variations in polymer structure.

scientific article published on 30 September 2016

Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.

scientific article published on 19 July 2016

Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer

scientific article

Micro-volume couette flow sample orientation for absorbance and fluorescence linear dichroism

scientific article published in September 2004

Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis.

scholarly article

Molecular studies of anti-HLA-A2 using light-chain shuffling: a structural model for HLA antibody binding.

scientific article published in April 2004

Multifunctional graphene oxide-bacteriophage based porous three-dimensional micro-nanocomposites

scientific article published on 04 July 2019

Murine serpin 2A is a redox-sensitive intracellular protein.

scientific article published on April 2003

Mutation of M13 Bacteriophage Major Coat Protein for Increased Conjugation to Exogenous Compounds

scientific article published on 01 June 2018

Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ

scientific article published on 10 December 2019

Natively unfolded domains in endocytosis: hooks, lines and linkers.

scientific article

Neutrophil elastase reduces secretion of secretory leukoproteinase inhibitor (SLPI) by lung epithelial cells: role of charge of the proteinase-inhibitor complex.

scientific article published on 12 August 2008

Nucleation of alpha 1-antichymotrypsin polymerization

scientific article published in March 2003

Physical characterization of serpin conformations

scientific article

Predicting HLA class I alloantigen immunogenicity from the number and physiochemical properties of amino acid polymorphisms

scientific article published in September 2009

Protochlorophyllide oxidoreductase: a homology model examined by site-directed mutagenesis

scientific article published in August 2001

Purification of membrane proteins free from conventional detergents: SMA, new polymers, new opportunities and new insights.

scientific article published on 30 March 2018

Rapid injection linear dichroism for studying the kinetics of biological processes.

scientific article published on 18 July 2012

Restriction Enzyme Kinetics Monitored by UV Linear Dichroism†

scientific article published on 01 July 2006

SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis

scientific article published on 28 May 2019

Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition.

scientific article

So how do you know you have a macromolecular complex?

scientific article published on 13 December 2006

Structural Implications of Platelet Endothelial Cell Adhesion Molecule-1 Polymorphisms

scientific article published on 01 August 2008

Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins

scientific article published on 23 October 2014

Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53

scientific article

Structural basis of substrate specificity in malate dehydrogenases: crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, alpha-ketomalonate and tetrahydoNAD

scientific article

Structural characterization of recombinant human CD81 produced in Pichia pastoris

scientific article published on 24 October 2007

Structures of the dI2dIII1 complex of proton-translocating transhydrogenase with bound, inactive analogues of NADH and NADPH reveal active site geometries

scientific article

Substitution of tyrosine 146 in the dI component of proton-translocating transhydrogenase leads to reversible dissociation of the active dimer into inactive monomers

scientific article published on 2 October 2007

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a.

scientific article

Surfactant-free purification of membrane proteins with intact native membrane environment

scientific article published on June 2011

Synchrotron radiation linear dichroism spectroscopy of the antibiotic peptide gramicidin in lipid membranes.

scientific article published on 19 May 2009

Targeting a surface cavity of alpha 1-antitrypsin to prevent conformational disease

scientific article

Tetramerization of ZapA is required for FtsZ bundling.

scientific article published in February 2013

The N‐terminal segment of antithrombin acts as a steric gate for the binding of heparin ⬇️

scientific article published on March 1, 1998

The desmoglein-specific cytoplasmic region is intrinsically disordered in solution and interacts with multiple desmosomal protein partners

scientific article published on 30 December 2008

The influence of poly(styrene-co-maleic acid) copolymer structure on the properties and self-assembly of SMALP nanodiscs

scientific article published on 22 December 2017

The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis

scientific article (publication date: 28 December 2001)

The pH sensitivity of murine heat shock protein 47 (HSP47) binding to collagen is affected by mutations in the breach histidine cluster.

scientific article published on 4 December 2012

The synergistic action of melittin and phospholipase A2 with lipid membranes: development of linear dichroism for membrane-insertion kinetics.

scientific article

The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy. ⬇️

scientific article

Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure. ⬇️

scientific article

Validation of new microvolume Couette flow linear dichroism cells

scientific article published on 14 October 2005

α-1-antitrypsin variants and the proteinase/antiproteinase imbalance in chronic obstructive pulmonary disease

scientific article published in January 2015

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