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List of works by Hiroshi Ueda

"Quenchbodies": quench-based antibody probes that show antigen-dependent fluorescence

scientific article published on 06 October 2011

A Protein-Protein Interaction Assay FlimPIA Based on the Functional Complementation of Mutant Firefly Luciferases

scientific article published in January 2016

A general method to select antibody fragments suitable for noncompetitive detection of monovalent antigens.

scientific article

A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction

scientific article published in November 2002

A homogeneous noncompetitive immunoassay for the detection of small haptens

scientific article

A new gibberellin detection system in living cells based on antibody V(H)/V(L) interaction.

scientific article

A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases.

scientific article published on August 2013

A signal-on fluorosensor based on quench-release principle for sensitive detection of antibiotic rapamycin.

scientific article

AMEGA: antigen-mediated genetically modified cell amplification.

scientific article published on January 2004

Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules

scientific article published on 29 March 2011

An open sandwich immunoassay for detection of 13(R,S)-hydroxy-9(E),11(E)-octadecadienoic acid.

scientific article

An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation

scientific article published in August 2003

Antibody Fab display system that can perform open-sandwich ELISA

scientific article published on 07 December 2008

Antibody affinity maturation in vitro using unconjugated peptide antigen

scientific article

Antigen responsive antibody-receptor kinase chimera

scientific article published on 01 April 1992

Array-based rational design of short peptide probe derived from an anti-TNT monoclonal antibody.

scientific article published on 28 August 2017

Characterization and fed-batch culture of hybridoma overexpressing apoptosis suppressing gene bcl-2.

scientific article published on July 1997

Comparison of the efficiencies of different affinity tags in the purification of a recombinant secretory protein expressed in silkworm larval hemolymph

Construction of Quenchbodies to detect and image amyloid β oligomers

scientific article published on 17 April 2018

Construction of dye-stapled Quenchbodies by photochemical crosslinking to antibody nucleotide-binding sites

scientific article

Covalent split protein fragment-DNA hybrids generated through N-terminus-specific modification of proteins by oligonucleotides

scientific article

Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains

scientific article published in January 2017

Creation of a Ligand-Dependent Enzyme by Fusing Circularly Permuted Antibody Variable Region Domains

scientific article published on 29 February 2016

Creation of a Nanobody-Based Fluorescent Immunosensor Mini Q-body for Rapid Signal-On Detection of Small Hapten Methotrexate

scientific article published on 10 November 2020

Creation of a thermostable firefly luciferase with pH-insensitive luminescent color

scientific article

Cyclic RGD peptide-labeled upconversion nanophosphors for tumor cell-targeted imaging.

scientific article published on 8 February 2009

Death signalobody: inducing conditional cell death in response to a specific antigen.

scientific article published on 21 April 2013

Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer.

scientific article

Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments.

scientific article published on 28 March 2013

Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex

scientific article published on 10 February 2020

Detection of protein tyrosine phosphorylation by open sandwich fluoroimmunoassay

scientific article

Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein

scientific article published in January 2002

Detection of small molecule diagnostic markers with phage-based open-sandwich immuno-PCR.

scientific article published on 17 January 2012

Detection of vimentin serine phosphorylation by multicolor Quenchbodies.

scientific article published on 23 June 2012

Development of a Quenchbody for the Detection and Imaging of the Cancer-Related Tight-Junction-Associated Membrane Protein Claudin

scientific article

Development of a fluorescent protein-antibody Förster resonance energy transfer probe for the detection and imaging of osteocalcin

scientific article published on 12 October 2016

Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology

article

Dimerization-based homogeneous fluorosensor proteins for the detection of specific dsDNA.

scientific article published on 29 November 2007

Direct construction of an open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4.

scientific article

ELISA-type assays of trace biomarkers using microfluidic methods

scientific article published on 20 February 2017

Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production.

scientific article published on 8 February 2016

Engineering of Functional Chimeric Protein G–VargulaLuciferase ⬇️

scientific article published on July 1, 1997

Enhanced expression of neuronal nitric oxide synthase in islets of exercise-trained rats

scientific article published in December 2003

Enhanced fluorescence resonance energy transfer immunoassay with improved sensitivity based on the Fab'-based immunoconjugates

scientific article published on 7 November 2006

Enzymatic labeling of a single chain variable fragment of an antibody with alkaline phosphatase by microbial transglutaminase

scientific article

Establishment of an apoptosis-resistant and growth-controllable cell line by transfecting with inducible antisense c-Jun gene.

scientific article published on April 1998

Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage

scientific article published on 11 October 2018

Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome

scientific article published on 7 December 2005

Expression of an antibody-enzyme complex by the L-chain fusion method.

scientific article published on 14 February 2013

Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells ⬇️

scientific article published on August 6, 2010

Flashbody: A Next Generation Fluobody with Fluorescence Intensity Enhanced by Antigen Binding

scientific article

Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin.

scientific article published in December 2008

From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins.

scientific article

Graphene Field Effect Transistor-Based Immunosensor for Ultrasensitive Noncompetitive Detection of Small Antigens

scientific article published on 15 January 2020

Green Fluorescent Protein-Based Glucose Indicators Report Glucose Dynamics in Living Cells

scientific article published on 22 March 2019

Growth control of genetically modified cells using an antibody/c-Kit chimera.

scientific article published on 9 January 2012

Growth promotion of genetically modified hematopoietic progenitors using an antibody/c-Mpl chimera.

scientific article published on 22 June 2011

Growth rate suppression of cultured mammalian cells enhances protein productivity.

scientific article published on January 1994

Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation

scientific article published on 15 February 2018

Homogeneous sandwich immunoassay based on the enzymatic complementation induced by single-chain Fv fragments

scientific article published in April 2004

Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure

scientific article published on 15 October 2008

Human single-chain antibody expression in the hemolymph and fat body of silkworm larvae and pupae using BmNPV bacmids

scientific article published on January 2009

Importance of terminal residues on circularly permutated Escherichia coli alkaline phosphatase with high specific activity.

scientific article

Improved detection sensitivity and selectivity attained by open-sandwich selection of an anti-estradiol antibody

scientific article published on 01 January 2012

Improved growth response of antibody/receptor chimera attained by the engineering of transmembrane domain.

scientific article

Improved protein-protein interaction assay FlimPIA by the entrapment of luciferase conformation

scientific article published on 26 December 2013

Improved secretion of molecular chaperone-assisted human IgG in silkworm, and no alterations in their N-linked glycan structures

scientific article published on January 2010

Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity.

scientific article published on 28 July 2017

Insight into the Working Mechanism of Quenchbody: Transition of the Dye around Antibody Variable Region That Fluoresces upon Antigen Binding

scientific article published on 20 September 2016

Intrabody-based FRET probe to visualize endogenous histone acetylation

scientific article published on 15 July 2019

Kinetics and binding sites for interaction of the prefoldin with a group II chaperonin: contiguous non-native substrate and chaperonin binding sites in the archaeal prefoldin.

scientific article

Luminescent and substrate binding activities of firefly luciferase N-terminal domain

scientific article published in July 2003

Mathematical Model of the Firefly Luciferase Complementation Assay Reveals a Non-Linear Relationship between the Detected Luminescence and the Affinity of the Protein Pair Being Analyzed

scientific article published on 17 February 2016

Micro OS-ELISA: Rapid noncompetitive detection of a small biomarker peptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device

scientific article

Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples.

scientific article

N-butylamine functionalized graphene oxide for detection of iron(III) by photoluminescence quenching.

scientific article published on 27 May 2015

Non-Steady State Analysis of Enzyme Kinetics in Real Time Elucidates Substrate Association and Dissociation Rates: Demonstration with Analysis of Firefly Luciferase Mutants

scientific article published on 24 May 2019

Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay

scientific article published on 18 July 2007

Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation.

scientific article published on 10 April 2018

Novel site-specific immobilization of a functional protein using a preferred substrate sequence for transglutaminase 2

scientific article published on 17 June 2007

Open Sandwich Enzyme-Linked Immunosorbent Assay for the Quantitation of Small Haptens

scientific article published on 01 November 2000

Open flower fluoroimmunoassay: a general method to make fluorescent protein-based immunosensor probes

scientific article published on 26 February 2015

Open sandwich immunoassay: a novel immunoassay approach based on the interchain interaction of an antibody variable region

scientific article published on 01 January 2002

Open sandwich-based immuno-transistor for label-free and noncompetitive detection of low molecular weight antigen

scientific article published in September 2009

Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol

scientific article published in October 2009

Open-sandwich immunoassay for sensitive and broad-range detection of a shellfish toxin gonyautoxin.

scientific article

Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments

scientific article published on 14 June 2012

Optimal fusion of antibody binding domains resulted in higher affinity and wider specificity.

scientific article published on 22 April 2015

Overexpression of bcl-2, apoptosis suppressing gene: Prolonged viable culture period of hybridoma and enhanced antibody production

scientific article published on 01 October 1995

Preparation of Quenchbodies by protein transamination reaction

scientific article published on 19 January 2016

Production of scFv-displaying BmNPV in silkworm larvae and its efficient purification

scientific article

Protein-based open sandwich immuno-PCR for sensitive detection of small biomarkers

scientific article published in January 2013

Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay

scientific article published on 28 April 2018

Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.

scientific article

Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily.

scientific article published in August 2004

Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera.

scientific article

Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera.

scientific article published on January 2002

Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera.

scientific article

Sensitive detection of estrogenic mycotoxin zearalenone by open sandwich immunoassay

scientific article

Sensitive noncompetitive measurement of small molecules by open sandwich immunoassay

scientific article

Simultaneous retention of thermostability and specific activity in chimeric human alkaline phosphatases.

scientific article published in October 2014

Single cell reporter assay using cell surface displayed Vargula luciferase

scientific article published on 01 January 2001

Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies

article by Hee-Jin Jeong et al published 23 December 2018 in Sensors

Site-Selective Protein Chemical Modification of Exposed Tyrosine Residues Using Tyrosine Click Reaction

scientific article published on 13 April 2020

Strategy for making a superior Quenchbody to proteins: effect of the fluorophore position.

scientific article published on 23 July 2014

T cell growth control using hapten-specific antibody/interleukin-2 receptor chimera.

scientific article

The influence of domain structures on the signal transduction of chimeric receptors derived from the erythropoietin receptor.

scientific article

The role of firefly luciferase C-terminal domain in efficient coupling of adenylation and oxidative steps

scientific article published in August 2005

Trans-splicing as a novel method to rapidly produce antibody fusion proteins.

scientific article

Ultra Q-bodies: quench-based antibody probes that utilize dye-dye interactions with enhanced antigen-dependent fluorescence

scientific article

Ultra sensitive firefly luciferase-based protein-protein interaction assay (FlimPIA) attained by hinge region engineering and optimized reaction conditions.

scientific article published on 18 September 2015

Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases

scientific article published in January 2017

Verification of a specific reaction between an airborne antigen and an immobilized antibody at a gas-solid interface

scientific article published on 01 September 2009

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