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List of works by Juan M. Bolivar

A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

scientific article

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices.

scientific article published on 31 January 2018

Biotransformations in microstructured reactors: more than flowing with the stream?

scientific article published on 04 May 2011

Characterization and further stabilization of a new anti-prelog specific alcohol dehydrogenase from Thermus thermophilus HB27 for asymmetric reduction of carbonyl compounds

scientific article published on October 17, 2011

Coating of soluble and immobilized enzymes with ionic polymers: full stabilization of the quaternary structure of multimeric enzymes.

scientific article published in April 2009

Confocal Luminescence Lifetime Imaging with Variable Scan Velocity and Its Application to Oxygen Sensing

scientific article

Convenient microtiter plate-based, oxygen-independent activity assays for flavin-dependent oxidoreductases based on different redox dyes.

scientific article

Correction to: Immobilization of Enzymes and Cells

scientific article published on 01 January 2020

Development of a fully integrated falling film microreactor for gas-liquid-solid biotransformation with surface immobilized O2 -dependent enzyme

scientific article published in March 2016

Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts.

scientific article

Enzyme Immobilization in Wall-Coated Flow Microreactors

scientific article published on 01 January 2020

Immobilization and stabilization of a cyclodextrin glycosyltransferase by covalent attachment on highly activated glyoxyl-agarose supports

scientific article published on 01 July 2006

Immobilization-stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus.

scientific article published on 11 June 2008

Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers

scientific article published on 6 September 2009

Improvement of enzyme properties with a two-step immobilizaton process on novel heterofunctional supports

scientific article published on 14 October 2010

Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose

scientific article published on 13 March 2006

Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

scientific article published on 01 January 2020

Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

scientific article

Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate

scientific article published on August 4, 2011

Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes

scientific article published on 01 January 2020

New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme

scientific article

One-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Immobilized "Native Enzymes"

scientific article published on 01 January 2020

Oriented Coimmobilization of Oxidase and Catalase on Tailor-Made Ordered Mesoporous Silica

scientific article published on 2 May 2017

Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Z basic2: design of a heterogeneous D-amino acid oxidase catalyst on porous glass.

scientific article

Oriented covalent immobilization of antibodies onto heterofunctional agarose supports: a highly efficient immuno-affinity chromatography platform

scientific article published on 11 September 2012

Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports

scientific article

Positively charged mini-protein Zbasic2 as a highly efficient silica binding module: opportunities for enzyme immobilization on unmodified silica supports

scientific article published on 22 June 2012

Preparation of lipase-coated, stabilized, hydrophobic magnetic particles for reversible conjugation of biomacromolecules.

scientific article published on 18 February 2013

Process intensification for O2 -dependent enzymatic transformations in continuous single-phase pressurized flow

scientific article published on 08 January 2019

Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

scientific article published in February 2017

Quantitating intraparticle O2 gradients in solid supported enzyme immobilizates: experimental determination of their role in limiting the catalytic effectiveness of immobilized glucose oxidase

scientific article published on 07 March 2013

Reactivation of a thermostable lipase by solid phase unfolding/refolding effect of cysteine residues on refolding efficiency

scientific article published on 30 June 2011

Selective adsorption of small proteins on large-pore anion exchangers coated with medium size proteins.

scientific article published on 6 March 2010

Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts.

scientific article published on 04 February 2013

Simultaneous Determination of Oxygen and pH Inside Microfluidic Devices Using Core-Shell Nanosensors

scientific article published on 9 September 2016

Stabilization of a formate dehydrogenase by covalent immobilization on highly activated glyoxyl-agarose supports

scientific article published in March 2006

Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups.

scientific article published in January 2013

Synthesis of propyl gallate by transesterification of tannic acid in aqueous media catalysed by immobilised derivatives of tannase from Lactobacillus plantarum

scientific article published on 26 February 2011

The Microenvironment in Immobilized Enzymes: Methods of Characterization and Its Role in Determining Enzyme Performance

scientific article published on 24 September 2019

The Science of Enzyme Immobilization

scientific article published on 01 January 2020