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List of works by José R. Botella

A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in

article

A mechanical strain-induced 1-aminocyclopropane-1-carboxylic acid synthase gene.

scientific article

A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis.

scientific article published on 10 December 2015

A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants.

scientific article

A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum).

scientific article

Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection

scientific article published on 6 December 2017

An easy-to-perform, culture-free Campylobacter point-of-management assay for processing plant applications

scientific article published on 04 December 2019

Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

scientific article

Biochemical characterization of Arabidopsis developmentally regulated G-proteins (DRGs).

scientific article

Biosynthesis of DHGA and its roles in Arabidopsis seedling establishment

scientific article published on 16 April 2019

COP1 Promotes ABA-Induced Stomatal Closure by Modulating the Abundance of ABI/HAB and AHG3 Phosphatases

scientific article published on 13 October 2020

Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata).

scientific article published on March 1996

Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress.

scientific article published on 17 January 2018

Characterization of ATDRG1, a member of a new class of GTP-binding proteins in plants.

scientific article

Characterization of a strong, constitutive mung bean (Vigna radiata L.) promoter with a complex mode of regulation in planta

scientific article published in December 2005

Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.).

scientific article

Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division

scientific article published on 19 August 2005

Developing pineapple fruit has a small transcriptome dominated by metallothionein.

scientific article

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis.

scientific article

Different Characteristics of the Two Glutamate Synthases in the Green Leaves of Lycopersicon esculentum

scientific article published on 01 December 1987

Differential expression of two calmodulin genes in response to physical and chemical stimuli.

scientific article published on March 1994

Dissecting Arabidopsis Gβ signal transduction on the protein surface

scientific article published on 08 May 2012

Diversity of heterotrimeric G-protein γ subunits in plants

scientific article

Down-regulation of Fusarium oxysporum endogenous genes by Host-Delivered RNA interference enhances disease resistance

scientific article published on 20 January 2015

Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis

scientific article published on 22 December 2014

Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1.

scientific article published on 10 May 2007

Field Demonstration of a Multiplexed Point-of-Care Diagnostic Platform for Plant Pathogens

scientific article

Functional Characterization of Novel Chitinase Genes Present in the Sheath Blight Resistance QTL: qSBR11-1 in Rice Line Tetep

scientific article published on March 2016

Fusarium oxysporum infection assays in Arabidopsis

scientific article

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

scientific article published on 15 March 2017

Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties

scientific article published on 6 December 2017

Genome Editing in Cotton with the CRISPR/Cas9 System

scientific article published on 3 August 2017

Ggamma1 + Ggamma2 not equal to Gbeta: heterotrimeric G protein Ggamma-deficient mutants do not recapitulate all phenotypes of Gbeta-deficient mutants.

scientific article published on 25 April 2008

Gγ1+Gγ2+Gγ3=Gβ: the search for heterotrimeric G-protein γ subunits in Arabidopsis is over

scientific article published on 29 December 2011

Heritability of targeted gene modifications induced by plant-optimized CRISPR systems

scientific article published on 27 September 2016

Heterotrimeric G protein gamma subunits provide functional selectivity in Gbetagamma dimer signaling in Arabidopsis.

scientific article published on 27 April 2007

Heterotrimeric G protein signalling in the plant kingdom.

scientific article published on 27 March 2013

Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling

scientific article published on 9 December 2005

Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis.

scientific article published on 24 September 2015

Heterotrimeric G proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases.

scientific article published on 19 February 2013

Heterotrimeric G proteins-mediated resistance to necrotrophic pathogens includes mechanisms independent of salicylic acid-, jasmonic acid/ethylene- and abscisic acid-mediated defense signaling.

scientific article published on 29 December 2008

Heterotrimeric G-proteins facilitate resistance to plant pathogenic viruses in Arabidopsis thaliana (L.) Heynh.

scientific article published on 25 July 2016

Host Delivered RNAi, an efficient approach to increase rice resistance to sheath blight pathogen (Rhizoctonia solani).

scientific article

Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes

article

Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid.

scientific article published on November 1992

Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments

scientific article

Identification of two new members of the 1-aminocyclopropane-1-carboxylate synthase-encoding multigene family in mung bean

scientific article published in January 1993

Immunochemical comparison of glutamine synthetases from some solanaceae plants

scientific article published on 01 October 1986

Immunocytolocalization of Ferredoxin-GOGAT in the Cells of Green Leaves and Cotyledons of Lycopersicon esculentum

scientific article published on 01 May 1988

Increased plant volatile production affects oviposition, but not larval development, in the moth Helicoverpa armigera

scientific article published on November 2011

Isolation of a cDNA Clone for Spinach Lipid Transfer Protein and Evidence that the Protein Is Synthesized by the Secretory Pathway.

scientific article

Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with Gbeta

scientific article published in August 2001

Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems

scientific article published on 28 September 2018

Membrane-localized extra-large G proteins and Gbg of the heterotrimeric G proteins form functional complexes engaged in plant immunity in Arabidopsis.

scientific article published in March 2015

Microarray analysis of gene expression profiles in ripening pineapple fruits

scientific article

Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels

scientific article published in April 2005

Molecular inversion probe: a new tool for highly specific detection of plant pathogens

scientific article

Novel Chitinase Gene LOC_Os11g47510 from Indica Rice Tetep Provides Enhanced Resistance against Sheath Blight Pathogen Rhizoctonia solani in Rice

scientific article

Now for the hard ones: is there a limit on CRISPR genome editing in crops?

scientific article published on 01 February 2019

Nucleic acid purification from plants, animals and microbes in under 30 seconds.

scientific article published on 21 November 2017

Nucleotide exchange-dependent and nucleotide exchange-independent functions of plant heterotrimeric GTP-binding proteins

scientific article published on 05 November 2019

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Over-expression of a truncated Arabidopsis thaliana heterotrimeric G protein gamma subunit results in a phenotype similar to alpha and beta subunit knockouts.

scientific article published on 24 February 2007

Phylogenomic and functional analysis of pterin-4a-carbinolamine dehydratase family (COG2154) proteins in plants and microorganisms.

scientific article

Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana

scientific article

PineappleDB: an online pineapple bioinformatics resource.

scientific article

Plant G-Proteins Come of Age: Breaking the Bond with Animal Models.

scientific article published on 24 May 2016

Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks

scientific article published on 01 October 2020

Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings

scientific article published on 26 January 2015

Recent advances in the development of transgenic papaya technology.

scientific article published on January 2008

Saltational evolution of the heterotrimeric G protein signaling mechanisms in the plant kingdom.

scientific article published on 20 September 2016

Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits

scientific article published in May 2017

Signaling specificity provided by the Arabidopsis thaliana heterotrimeric G-protein γ subunits AGG1 and AGG2 is partially but not exclusively provided through transcriptional regulation

scientific article

Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.].

scientific article

Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus.

scientific article published on January 2016

Site-directed mutagenesis of the Arabidopsis heterotrimeric G protein β subunit suggests divergent mechanisms of effector activation between plant and animal G proteins.

scientific article published on 15 October 2011

Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes

scientific article

TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice

scientific article

The 5' untranslated region of the VR-ACS1 mRNA acts as a strong translational enhancer in plants

scientific article published on 9 October 2009

The gland localized CGP1 controls gland pigmentation and gossypol accumulation in cotton

scientific article published on 28 December 2019

The pineapple AcMADS1 promoter confers high level expression in tomato and Arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant

scientific article

Tobacco isoenzyme 1 of NAD(H)-dependent glutamate dehydrogenase catabolizes glutamate in vivo

scientific article published on 17 November 2006

Type B Heterotrimeric G Protein γ-Subunit Regulates Auxin and ABA Signaling in Tomato.

scientific article published on 14 December 2015

VERNALIZATION1 Modulates Root System Architecture in Wheat and Barley.

scientific article published on 19 October 2017

Whole Genome Sequencing Reveals Potential New Targets for Improving Nitrogen Uptake and Utilization in Sorghum bicolor

scientific article

Whole-Genome Analysis of Candidate genes Associated with Seed Size and Weight in Sorghum bicolor Reveals Signatures of Artificial Selection and Insights into Parallel Domestication in Cereal Crops

scientific article

Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.

scientific article published in January 2016

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