List of works - Alvaro Martínez-del-Pozo

1H, 13C, and 15N NMR assignments of StnII-R29Q, a defective lipid binding mutant of the sea anemone actinoporin Sticholysin II

scientific article published on 01 December 2009

1H, 13C, and 15N NMR assignments of StnII-Y111N, a highly impaired mutant of the sea anemone actinoporin Sticholysin II.

scientific article

2NH and 3OH are crucial structural requirements in sphingomyelin for sticholysin II binding and pore formation in bilayer membranes.

scientific article published on 30 January 2013

A deletion variant of the Aspergillus fumigatus ribotoxin Asp f 1 induces an attenuated airway inflammatory response in a mouse model of sensitization

scientific article published on 01 January 2010

A non-cytotoxic but ribonucleolytically specific ribotoxin variant: implication of tryptophan residues in the cytotoxicity of hirsutellin A.

scientific article published on May 2012

A novel Carcinoembryonic Antigen (CEA)-Targeted Trimeric Immunotoxin shows significantly enhanced Antitumor Activity in Human Colorectal Cancer Xenografts

scientific article published on 12 August 2019

A peptide of nine amino acid residues from alpha-sarcin cytotoxin is a membrane-perturbing structure

scientific article published on 01 February 1998

A protein from the mold Aspergillus giganteus is a potent inhibitor of fungal plant pathogens

scientific article published in November 2001

Activity and spectroscopic properties of bacterial D-amino acid transaminase after multiple site-directed mutagenesis of a single tryptophan residue

scientific article published on 01 January 1989

Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2.

scientific article published in September 2005

Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin

scientific article published in December 2001

Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin alpha-sarcin

scientific article published on 01 November 2000

Backbone dynamics of the cytotoxic ribonuclease alpha-sarcin by 15N NMR relaxation methods.

scientific article

Biotechnologically relevant enzymes and proteins. Antifungal mechanism of the Aspergillus giganteus AFP against the rice blast fungus Magnaporthe grisea

scientific article published on 24 March 2006

Calorimetric scrutiny of lipid binding by sticholysin II toxin mutants

scientific article published on 26 July 2008

Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus

scientific article published on 01 June 1997

Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin

scientific article published on 29 July 2016

Characterization of a novel cysteine-rich antifungal protein from Fusarium graminearum with activity against maize fungal pathogens

scientific article published on 25 June 2018

Characterization of pKa values and titration shifts in the cytotoxic ribonuclease alpha-sarcin by NMR. Relationship between electrostatic interactions, structure, and catalytic function.

scientific article published in November 1998

Characterization of the antifungal protein secreted by the mould Aspergillus giganteus

scientific article

Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes.

scientific article

Cleavage of the sarcin-ricin loop of 23S rRNA differentially affects EF-G and EF-Tu binding

scientific article

Conformational study of the antitumor protein α-sarcin

scientific article published on 01 April 1988

Conserved asparagine residue 54 of alpha-sarcin plays a role in protein stability and enzyme activity

scientific article published in December 2004

Control of polygalacturonase synthesis in Fusarium oxyspotum f.sp. radicis lycopersici

scientific article published on 01 November 1997

Deletion of the NH2-terminal beta-hairpin of the ribotoxin alpha-sarcin produces a nontoxic but active ribonuclease.

scientific article

Differential Effect of Bilayer Thickness on Sticholysin Activity.

scientific article published on 21 September 2017

Differential Effect of Membrane Composition on the Pore-Forming Ability of Four Different Sea Anemone Actinoporins.

scientific article published on 22 November 2016

Differential toxicity of antifungal protein AFP against mutants of Fusarium oxysporum.

scientific article published in June 2009

Dissecting structural and electrostatic interactions of charged groups in alpha-sarcin. An NMR study of some mutants involving the catalytic residues.

scientific article published in November 2003

Effect of the antitumour protein α-sarcin on the thermotropic behaviour of acid phospholipid vesicles

scientific article published on 01 September 1991

Effects of D-serine on bacterial D-amino acid transaminase: accumulation of an intermediate and inactivation of the enzyme

scientific article published on 01 October 1989

Efficient in vivo antitumor effect of an immunotoxin based on ribotoxin α-sarcin in nude mice bearing human colorectal cancer xenografts.

scientific article published on 8 April 2015

Escherichia coli JA221 can suppress the UAG stop signal.

scientific article

Food mustard allergen interaction with phospholipid vesicles

scientific article published on 01 October 1994

Functional and Structural Variation among Sticholysins, Pore-Forming Proteins from the Sea Anemone <i>Stichodactyla helianthus</i>

scientific article published on 24 November 2020

Fungal Ribotoxins: A Review of Potential Biotechnological Applications.

scientific article published on 21 February 2017

Fungal extracellular ribotoxins as insecticidal agents

scientific article

Fungal ribotoxins: Natural protein-based weapons against insects

scientific article published on 14 March 2014

Hirsutellin A Displays Significant Homology to Microbial Extracellular Ribonucleases

scientific article published on 01 July 1999

Implication of an Asp residue in the ribonucleolytic activity of hirsutellin A reveals new electrostatic interactions at the active site of ribotoxins

scientific article published on 24 August 2011

Inactivation of dimeric D-amino acid transaminase by a normal substrate through formation of an unproductive coenzyme adduct in one subunit

scientific article published on 01 July 1992

Influence of key residues on the heterologous extracellular production of fungal ribonuclease U2 in the yeast Pichia pastoris

scientific article

Interaction of type I collagen fibrils with phospholipid vesicles

scientific article published on 01 November 1989

Interaction of type I collagen with phosphatidylcholine vesicles

scientific article published on 01 March 1988

Intrinsic local disorder and a network of charge-charge interactions are key to actinoporin membrane disruption and cytotoxicity

scientific article

Involvement of loop 5 lysine residues and the N-terminal β-hairpin of the ribotoxin hirsutellin A on its insecticidal activity.

scientific article published on 19 November 2015

Involvement of loops 2 and 3 of α-sarcin on its ribotoxic activity.

scientific article published on 15 January 2015

Leucine 145 of the ribotoxin alpha-sarcin plays a key role for determining the specificity of the ribosome-inactivating activity of the protein.

scientific article published on January 2003

Minimized natural versions of fungal ribotoxins show improved active site plasticity.

scientific article published on 7 March 2017

Mitogillin and related fungal ribotoxins

scientific article published on 01 January 2001

Modulation by the ratio S-adenosylmethionineS-adenosylhomocysteine of cyclic AMP-dependent phosphorylation of the 50 kDa protein of rat liver phospholipid methyltransferase

scientific article published in December 1985

NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism

scientific article

NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.

scientific article published in April 2004

Na+ controls hypoxic signalling by the mitochondrial respiratory chain

scientific article published on 29 July 2020

Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes

scientific article published on 01 July 1998

One single salt bridge explains the different cytolytic activities shown by actinoporins sticholysin I and II from the venom of Stichodactyla helianthus

scientific article published on 11 November 2017

Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.

scientific article

Overproduction in Escherichia coli and Purification of the Hemolytic Protein Sticholysin II from the Sea Anemone Stichodactyla helianthus

scientific article published on 01 February 2000

Partially folded states of the cytolytic protein sticholysin II.

scientific article

Phenotypic selection and characterization of randomly produced non-haemolytic mutants of the toxic sea anemone protein sticholysin II

article

Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools

scientific article published on 25 June 2019

Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin-membrane interaction

scientific article published on 01 February 1995

Preparation of an engineered safer immunotoxin against colon carcinoma based on the ribotoxin hirsutellin A.

scientific article published on 27 March 2015

Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding.

scientific article

Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells

scientific article published on 25 July 2012

Production of the biotechnologically relevant AFP from Aspergillus giganteus in the yeast Pichia pastoris

scientific article published on 5 November 2009

RNase U2 and alpha-sarcin: a study of relationships

scientific article published on 01 January 2001

Refined NMR structure of alpha-sarcin by 15N-1H residual dipolar couplings

scientific article published on 6 April 2005

Regulation of Sticholysin II-Induced Pore Formation by Lipid Bilayer Composition, Phase State, and Interfacial Properties

scientific article published on 22 March 2016

Release of Lipid Vesicle Contents by an Antibacterial Cecropin A−Melittin Hybrid Peptide†

scientific article published on 01 July 1996

Ribonuclease U2: cloning, production inPichia pastorisand affinity chromatography purification of the active recombinant protein

scientific article published on 01 August 2000

Ribotoxins are a more widespread group of proteins within the filamentous fungi than previously believed.

scientific article

Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin alpha-sarcin

scientific article published on 01 November 1999

Role of the Tryptophan Residues in the Specific Interaction of the Sea Anemone Stichodactyla helianthus's Actinoporin Sticholysin II with Biological Membranes

scientific article published on 10 November 2016

Role of the basic character of alpha-sarcin's NH2-terminal beta-hairpin in ribosome recognition and phospholipid interaction

scientific article

Role reversal for substrates and inhibitors. Slow inactivation of D-amino acid transaminase by its normal substrates and protection by inhibitors

scientific article published on 01 August 1993

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

scientific article

Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818.

scientific article published in July 1997

Silent mutations at the 5'-end of the cDNA of actinoporins from the sea anemone Stichodactyla helianthus allow their heterologous overproduction in Escherichia coli.

scientific article

Specific interactions of sticholysin I with model membranes: an NMR study.

scientific article

Spectroscopic characterization of the alkylated alpha-sarcin cytotoxin: analysis of the structural requirements for the protein-lipid bilayer hydrophobic interaction

scientific article published in September 1995

Stereospecificity of reactions catalyzed by bacterial D-amino acid transaminase

scientific article published on 01 October 1989

Stichodactyla helianthus' de novo transcriptome assembly: Discovery of a new actinoporin isoform

scientific article published on 19 May 2018

Sticholysin II, a cytolysin from the sea anemone Stichodactyla helianthus , is a monomer-tetramer associating protein

article

Sticholysin, Sphingomyelin, and Cholesterol: A Closer Look at a Tripartite Interaction

scientific article published on 16 May 2019

Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease α-sarcin

scientific article published on 01 December 1996

Structure-Activity Relationship of α Mating Pheromone from the Fungal Pathogen Fusarium oxysporum

scientific article

Substrate Inhibition of d-Amino Acid Transaminase and Protection by Salts and by Reduced Nicotinamide Adenine Dinucleotide: Isolation and Initial Characterization of a Pyridoxo Intermediate Related to Inactivation ⬇️

scientific article published on March 3, 1998

Synergistic Action of Actinoporin Isoforms from the Same Sea Anemone Species Assembled into Functionally Active Heteropores

scientific article published on 27 April 2016

Tautomeric state of alpha-sarcin histidines. Ndelta tautomers are a common feature in the active site of extracellular microbial ribonucleases.

scientific article published in January 2003

The acidic ribosomal stalk proteins are not required for the highly specific inactivation exerted by α-sarcin of the eukaryotic ribosome.

scientific article published on 4 March 2014

The behavior of sea anemone actinoporins at the water-membrane interface

scientific article published on 20 May 2011

The cytotoxin α-sarcin behaves as a cyclizing ribonuclease

scientific article published on 01 March 1998

The effect of cholesterol on the long-range network of interactions established among sea anemone Sticholysin II residues at the water-membrane interface

scientific article

The insecticidal protein hirsutellin A from the mite fungal pathogen Hirsutella thompsonii is a ribotoxin

scientific article published in July 2008

The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition

scientific article published on 13 July 2011

The sea anemone actinoporin (Arg-Gly-Asp) conserved motif is involved in maintaining the competent oligomerization state of these pore-forming toxins

scientific article published on 06 February 2014

The solubility of the ribotoxin alpha-sarcin, produced as a recombinant protein in Escherichia coli, is increased in the presence of thioredoxin

scientific article published on 01 April 2000

Thermal unfolding of the cytotoxin alpha-sarcin: phospholipid binding induces destabilization of the protein structure.

scientific article

Three-dimensional structure of the actinoporin sticholysin I. Influence of long-distance effects on protein function.

scientific article

Toxin-induced pore formation is hindered by intermolecular hydrogen bonding in sphingomyelin bilayers.

scientific article published on 11 March 2016

Translocation of alpha-sarcin across the lipid bilayer of asolectin vesicles.

scientific article published on October 1993

gamma-Acetylenic-gamma-aminobutyrate as an enzyme-activated inhibitor of D-amino acid transaminase

scientific article published on 01 April 1989

α-sarcin and RNase T1 based immunoconjugates: the role of intracellular trafficking in cytotoxic efficiency.

scientific article

List of works by Alvaro Martínez-del-Pozo

1H, 13C, and 15N NMR assignments of StnII-R29Q, a defective lipid binding mutant of the sea anemone actinoporin Sticholysin II

1H, 13C, and 15N NMR assignments of StnII-Y111N, a highly impaired mutant of the sea anemone actinoporin Sticholysin II.

2NH and 3OH are crucial structural requirements in sphingomyelin for sticholysin II binding and pore formation in bilayer membranes.

A deletion variant of the Aspergillus fumigatus ribotoxin Asp f 1 induces an attenuated airway inflammatory response in a mouse model of sensitization

A non-cytotoxic but ribonucleolytically specific ribotoxin variant: implication of tryptophan residues in the cytotoxicity of hirsutellin A.

A novel Carcinoembryonic Antigen (CEA)-Targeted Trimeric Immunotoxin shows significantly enhanced Antitumor Activity in Human Colorectal Cancer Xenografts

A peptide of nine amino acid residues from alpha-sarcin cytotoxin is a membrane-perturbing structure

A protein from the mold Aspergillus giganteus is a potent inhibitor of fungal plant pathogens

Activity and spectroscopic properties of bacterial D-amino acid transaminase after multiple site-directed mutagenesis of a single tryptophan residue

Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2.

Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin

Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin alpha-sarcin

Backbone dynamics of the cytotoxic ribonuclease alpha-sarcin by 15N NMR relaxation methods.

Biotechnologically relevant enzymes and proteins. Antifungal mechanism of the Aspergillus giganteus AFP against the rice blast fungus Magnaporthe grisea

Calorimetric scrutiny of lipid binding by sticholysin II toxin mutants

Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus

Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin

Characterization of a novel cysteine-rich antifungal protein from Fusarium graminearum with activity against maize fungal pathogens

Characterization of pKa values and titration shifts in the cytotoxic ribonuclease alpha-sarcin by NMR. Relationship between electrostatic interactions, structure, and catalytic function.

Characterization of the antifungal protein secreted by the mould Aspergillus giganteus

Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes.

Cleavage of the sarcin-ricin loop of 23S rRNA differentially affects EF-G and EF-Tu binding

Conformational study of the antitumor protein α-sarcin

Conserved asparagine residue 54 of alpha-sarcin plays a role in protein stability and enzyme activity

Control of polygalacturonase synthesis in Fusarium oxyspotum f.sp. radicis lycopersici

Deletion of the NH2-terminal beta-hairpin of the ribotoxin alpha-sarcin produces a nontoxic but active ribonuclease.

Differential Effect of Bilayer Thickness on Sticholysin Activity.

Differential Effect of Membrane Composition on the Pore-Forming Ability of Four Different Sea Anemone Actinoporins.

Differential toxicity of antifungal protein AFP against mutants of Fusarium oxysporum.

Dissecting structural and electrostatic interactions of charged groups in alpha-sarcin. An NMR study of some mutants involving the catalytic residues.

Effect of the antitumour protein α-sarcin on the thermotropic behaviour of acid phospholipid vesicles

Effects of D-serine on bacterial D-amino acid transaminase: accumulation of an intermediate and inactivation of the enzyme

Efficient in vivo antitumor effect of an immunotoxin based on ribotoxin α-sarcin in nude mice bearing human colorectal cancer xenografts.

Escherichia coli JA221 can suppress the UAG stop signal.

Food mustard allergen interaction with phospholipid vesicles

Functional and Structural Variation among Sticholysins, Pore-Forming Proteins from the Sea Anemone <i>Stichodactyla helianthus</i>

Fungal Ribotoxins: A Review of Potential Biotechnological Applications.

Fungal extracellular ribotoxins as insecticidal agents

Fungal ribotoxins: Natural protein-based weapons against insects

Hirsutellin A Displays Significant Homology to Microbial Extracellular Ribonucleases

Implication of an Asp residue in the ribonucleolytic activity of hirsutellin A reveals new electrostatic interactions at the active site of ribotoxins

Inactivation of dimeric D-amino acid transaminase by a normal substrate through formation of an unproductive coenzyme adduct in one subunit

Influence of key residues on the heterologous extracellular production of fungal ribonuclease U2 in the yeast Pichia pastoris

Interaction of type I collagen fibrils with phospholipid vesicles

Interaction of type I collagen with phosphatidylcholine vesicles

Intrinsic local disorder and a network of charge-charge interactions are key to actinoporin membrane disruption and cytotoxicity

Involvement of loop 5 lysine residues and the N-terminal β-hairpin of the ribotoxin hirsutellin A on its insecticidal activity.

Involvement of loops 2 and 3 of α-sarcin on its ribotoxic activity.

Leucine 145 of the ribotoxin alpha-sarcin plays a key role for determining the specificity of the ribosome-inactivating activity of the protein.

Minimized natural versions of fungal ribotoxins show improved active site plasticity.

Mitogillin and related fungal ribotoxins

Modulation by the ratio S-adenosylmethionineS-adenosylhomocysteine of cyclic AMP-dependent phosphorylation of the 50 kDa protein of rat liver phospholipid methyltransferase

NMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerism

NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.

Na+ controls hypoxic signalling by the mitochondrial respiratory chain

Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes

One single salt bridge explains the different cytolytic activities shown by actinoporins sticholysin I and II from the venom of Stichodactyla helianthus

Overproduction and purification of biologically active native fungal alpha-sarcin in Escherichia coli.

Overproduction in Escherichia coli and Purification of the Hemolytic Protein Sticholysin II from the Sea Anemone Stichodactyla helianthus

Partially folded states of the cytolytic protein sticholysin II.

Phenotypic selection and characterization of randomly produced non-haemolytic mutants of the toxic sea anemone protein sticholysin II

Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools

Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin-membrane interaction

Preparation of an engineered safer immunotoxin against colon carcinoma based on the ribotoxin hirsutellin A.

Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding.

Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells

Production of the biotechnologically relevant AFP from Aspergillus giganteus in the yeast Pichia pastoris

RNase U2 and alpha-sarcin: a study of relationships

Refined NMR structure of alpha-sarcin by 15N-1H residual dipolar couplings

Regulation of Sticholysin II-Induced Pore Formation by Lipid Bilayer Composition, Phase State, and Interfacial Properties

Release of Lipid Vesicle Contents by an Antibacterial Cecropin A−Melittin Hybrid Peptide†

Ribonuclease U2: cloning, production inPichia pastorisand affinity chromatography purification of the active recombinant protein

Ribotoxins are a more widespread group of proteins within the filamentous fungi than previously believed.

Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin alpha-sarcin

Role of the Tryptophan Residues in the Specific Interaction of the Sea Anemone Stichodactyla helianthus's Actinoporin Sticholysin II with Biological Membranes

Role of the basic character of alpha-sarcin's NH2-terminal beta-hairpin in ribosome recognition and phospholipid interaction

Role reversal for substrates and inhibitors. Slow inactivation of D-amino acid transaminase by its normal substrates and protection by inhibitors

Secretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.

Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818.