List of works - Niels Schaft

A GMP-compliant protocol to expand and transfect cancer patient T cells with mRNA encoding a tumor-specific chimeric antigen receptor

scientific article

A fast and robust method to clone and functionally validate T-cell receptors

scientific article

A new method to monitor antigen-specific CD8+ T cells, avoiding additional target cells and the restriction to human leukocyte antigen haplotype.

scientific article

An Altered gp100 Peptide Ligand with Decreased Binding by TCR and CD8α Dissects T Cell Cytotoxicity from Production of Cytokines and Activation of NFAT.

scientific article published on 04 September 2013

An improved method for RNA isolation and removal of melanin contamination from melanoma tissue: implications for tumor antigen detection and amplification

scientific article published on 16 May 2006

Autocrine TNF is critical for the survival of human dendritic cells by regulating BAK, BCL-2, and FLIPL.

scientific article

Autophagic degradation of lamins facilitates the nuclear egress of herpes simplex virus type 1

scientific article published on 26 December 2018

BRAF and MEK Inhibitors Influence the Function of Reprogrammed T Cells: Consequences for Adoptive T-Cell Therapy

scientific article published on 18 January 2018

Blockade of CCR7 leads to decreased dendritic cell migration to draining lymph nodes and promotes graft survival in low-risk corneal transplantation

scientific article published on 12 December 2015

CD8(+) T-cell priming and boosting: more antigen-presenting DC, or more antigen per DC?

scientific article published on 10 October 2013

CSPG4 as Target for CAR-T-Cell Therapy of Various Tumor Entities-Merits and Challenges

scientific article published on 26 November 2019

CSPG4-Specific CAR T Cells for High-Risk Childhood B Cell Precursor Leukemia

scientific article published on 05 June 2019

Clinical-Scale Production of CAR-T Cells for the Treatment of Melanoma Patients by mRNA Transfection of a CSPG4-Specific CAR under Full GMP Compliance

scientific article published on 16 August 2019

Combining a chimeric antigen receptor and a conventional T-cell receptor to generate T cells expressing two additional receptors (TETARs) for a multi-hit immunotherapy of melanoma

scientific article published in June 2016

Commonly used prophylactic vaccines as an alternative for synthetically produced TLR ligands to mature monocyte-derived dendritic cells

scientific article published on 27 April 2010

Concurrent interaction of DCs with CD4(+) and CD8(+) T cells improves secondary CTL expansion: It takes three to tango.

scientific article

Curatopes Melanoma: A Database of Predicted T-cell Epitopes from Overly Expressed Proteins in Metastatic Cutaneous Melanoma

scientific article published on 15 August 2019

Dendritic cell vaccination and other strategies to tip the balance of the immune system : DC2007 5th International Meeting, July 16-18, Bamberg, Germany

scientific article published on 31 January 2008

Effective clinical-scale production of dendritic cell vaccines by monocyte elutriation directly in medium, subsequent culture in bags and final antigen loading using peptides or RNA transfection

scientific article published in September 2007

Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells.

scientific article published on 28 December 2015

Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins

scientific article published in January 2017

Flexible and sensitive method to functionally validate tumor-specific receptors via activation of NFAT.

scientific article published in September 2003

Generation of CD8(+) T cells expressing two additional T-cell receptors (TETARs) for personalised melanoma therapy.

scientific article published on 15 July 2015

Generation of HIV-1-specific T cells by electroporation of T-cell receptor RNA.

scientific article published in August 2008

HIV-1 mRNA electroporation of PBMC: a simple and efficient method to monitor T-cell responses against autologous HIV-1 in HIV-1-infected patients

scientific article published on 3 April 2012

Human T cells expressing two additional receptors (TETARs) specific for HIV-1 recognize both epitopes

scientific article published on 16 September 2011

Human adenovirus-specific γ/δ and CD8+ T cells generated by T-cell receptor transfection to treat adenovirus infection after allogeneic stem cell transplantation

scientific article

In vivo induction of CTL responses by recombinant adenylate cyclase of Bordetella pertussis carrying multiple copies of a viral CD8(+) T-cell epitope

scientific article published on 01 November 1999

Introduction of functional chimeric E/L-selectin by RNA electroporation to target dendritic cells from blood to lymph nodes

scientific article published on 02 September 2007

Just one position-independent lysine residue can direct MelanA into proteasomal degradation following N-terminal fusion of ubiquitin

scientific article

Leukoreduction system chambers are an efficient, valid, and economic source of functional monocyte-derived dendritic cells and lymphocytes

scientific article published on 25 July 2013

Mild hyperthermia enhances human monocyte-derived dendritic cell functions and offers potential for applications in vaccination strategies.

scientific article published in January 2011

NF-κB activation triggers NK-cell stimulation by monocyte-derived dendritic cells

scientific article published on 11 December 2019

Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy.

scientific article published on January 2013

Norm- and hypo-fractionated radiotherapy is capable of activating human dendritic cells

scientific article published on 10 February 2014

O-linked N-acetylglucosaminylation of Sp1 inhibits the human immunodeficiency virus type 1 promoter

scientific article

Preclinical evaluation of NF-κB-triggered dendritic cells expressing the viral oncogenic driver of Merkel cell carcinoma for therapeutic vaccination.

scientific article

RNA-transfection of γ/δ T cells with a chimeric antigen receptor or an α/β T-cell receptor: a safer alternative to genetically engineered α/β T cells for the immunotherapy of melanoma.

scientific article published on 17 August 2017

Redirecting T cells to Ewing's sarcoma family of tumors by a chimeric NKG2D receptor expressed by lentiviral transduction or mRNA transfection

scientific article

Sarcoidosis Under Dendritic Cell Vaccination Immunotherapy in Long-term Responding Patients with Metastatic Melanoma

scientific article published in June 2017

Selection of adenovirus-specific and Epstein-Barr virus-specific T cells with major histocompatibility class I streptamers under Good Manufacturing Practice (GMP)-compliant conditions

scientific article published on 9 April 2015

Stability and activity of MCSP-specific chimeric antigen receptors (CARs) depend on the scFv antigen-binding domain and the protein backbone.

scientific article published on 29 October 2015

Strategies for immune intervention

scientific article published on 16 December 2005

Strategies for immune intervention; from bench to bedside: Symposium 2006 of the Collaborative Research Center "Strategies of cellular immune intervention" July 17th-18th, Erlangen, Germany

scientific article published on 29 March 2007

Strong and sustained effector function of memory- versus naïve-derived T cells upon T-cell receptor RNA transfer: implications for cellular therapy

scientific article published in October 2012

T Cell Telomere Length in HIV-1 Infection: No Evidence for Increased CD4+ T Cell Turnover

scientific article

T cell re-targeting to EBV antigens following TCR gene transfer: CD28-containing receptors mediate enhanced antigen-specific IFNgamma production

scientific article published on 28 February 2006

T-cell receptor transfer for boosting HIV-1-specific T-cell immunity in HIV-1-infected patients.

scientific article published on June 2016

Targeting of DEC-205 on human dendritic cells results in efficient MHC class II-restricted antigen presentation

scientific article published on 21 June 2010

The Landscape of CAR-T Cell Clinical Trials against Solid Tumors-A Comprehensive Overview

scientific article published on 09 September 2020

Transcriptional Targeting of Mature Dendritic Cells with Adenoviral Vectors via a Modular Promoter System for Antigen Expression and Functional Manipulation

scientific article published on 29 June 2016

Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells

scientific article published on 29 June 2015

Transfer of mRNA encoding recombinant immunoreceptors reprograms CD4+ and CD8+ T cells for use in the adoptive immunotherapy of cancer.

scientific article

Triggering of NF-κB in cytokine-matured human DCs generates superior DCs for T-cell priming in cancer immunotherapy

scientific article published on 16 September 2014

Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells.

scientific article published on 29 October 2012

List of works by Niels Schaft

A GMP-compliant protocol to expand and transfect cancer patient T cells with mRNA encoding a tumor-specific chimeric antigen receptor

A fast and robust method to clone and functionally validate T-cell receptors

A new method to monitor antigen-specific CD8+ T cells, avoiding additional target cells and the restriction to human leukocyte antigen haplotype.

An Altered gp100 Peptide Ligand with Decreased Binding by TCR and CD8α Dissects T Cell Cytotoxicity from Production of Cytokines and Activation of NFAT.

An improved method for RNA isolation and removal of melanin contamination from melanoma tissue: implications for tumor antigen detection and amplification

Autocrine TNF is critical for the survival of human dendritic cells by regulating BAK, BCL-2, and FLIPL.

Autophagic degradation of lamins facilitates the nuclear egress of herpes simplex virus type 1

BRAF and MEK Inhibitors Influence the Function of Reprogrammed T Cells: Consequences for Adoptive T-Cell Therapy

Blockade of CCR7 leads to decreased dendritic cell migration to draining lymph nodes and promotes graft survival in low-risk corneal transplantation

CD8(+) T-cell priming and boosting: more antigen-presenting DC, or more antigen per DC?

CSPG4 as Target for CAR-T-Cell Therapy of Various Tumor Entities-Merits and Challenges

CSPG4-Specific CAR T Cells for High-Risk Childhood B Cell Precursor Leukemia

Clinical-Scale Production of CAR-T Cells for the Treatment of Melanoma Patients by mRNA Transfection of a CSPG4-Specific CAR under Full GMP Compliance

Combining a chimeric antigen receptor and a conventional T-cell receptor to generate T cells expressing two additional receptors (TETARs) for a multi-hit immunotherapy of melanoma

Commonly used prophylactic vaccines as an alternative for synthetically produced TLR ligands to mature monocyte-derived dendritic cells

Concurrent interaction of DCs with CD4(+) and CD8(+) T cells improves secondary CTL expansion: It takes three to tango.

Curatopes Melanoma: A Database of Predicted T-cell Epitopes from Overly Expressed Proteins in Metastatic Cutaneous Melanoma

Dendritic cell vaccination and other strategies to tip the balance of the immune system : DC2007 5th International Meeting, July 16-18, Bamberg, Germany

Effective clinical-scale production of dendritic cell vaccines by monocyte elutriation directly in medium, subsequent culture in bags and final antigen loading using peptides or RNA transfection

Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells.

Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins

Flexible and sensitive method to functionally validate tumor-specific receptors via activation of NFAT.

Generation of CD8(+) T cells expressing two additional T-cell receptors (TETARs) for personalised melanoma therapy.

Generation of HIV-1-specific T cells by electroporation of T-cell receptor RNA.

HIV-1 mRNA electroporation of PBMC: a simple and efficient method to monitor T-cell responses against autologous HIV-1 in HIV-1-infected patients

Human T cells expressing two additional receptors (TETARs) specific for HIV-1 recognize both epitopes

Human adenovirus-specific γ/δ and CD8+ T cells generated by T-cell receptor transfection to treat adenovirus infection after allogeneic stem cell transplantation

In vivo induction of CTL responses by recombinant adenylate cyclase of Bordetella pertussis carrying multiple copies of a viral CD8(+) T-cell epitope

Introduction of functional chimeric E/L-selectin by RNA electroporation to target dendritic cells from blood to lymph nodes

Just one position-independent lysine residue can direct MelanA into proteasomal degradation following N-terminal fusion of ubiquitin

Leukoreduction system chambers are an efficient, valid, and economic source of functional monocyte-derived dendritic cells and lymphocytes

Mild hyperthermia enhances human monocyte-derived dendritic cell functions and offers potential for applications in vaccination strategies.

NF-κB activation triggers NK-cell stimulation by monocyte-derived dendritic cells

Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy.

Norm- and hypo-fractionated radiotherapy is capable of activating human dendritic cells

O-linked N-acetylglucosaminylation of Sp1 inhibits the human immunodeficiency virus type 1 promoter

Preclinical evaluation of NF-κB-triggered dendritic cells expressing the viral oncogenic driver of Merkel cell carcinoma for therapeutic vaccination.

RNA-transfection of γ/δ T cells with a chimeric antigen receptor or an α/β T-cell receptor: a safer alternative to genetically engineered α/β T cells for the immunotherapy of melanoma.

Redirecting T cells to Ewing's sarcoma family of tumors by a chimeric NKG2D receptor expressed by lentiviral transduction or mRNA transfection

Sarcoidosis Under Dendritic Cell Vaccination Immunotherapy in Long-term Responding Patients with Metastatic Melanoma

Selection of adenovirus-specific and Epstein-Barr virus-specific T cells with major histocompatibility class I streptamers under Good Manufacturing Practice (GMP)-compliant conditions

Stability and activity of MCSP-specific chimeric antigen receptors (CARs) depend on the scFv antigen-binding domain and the protein backbone.

Strategies for immune intervention

Strategies for immune intervention; from bench to bedside: Symposium 2006 of the Collaborative Research Center "Strategies of cellular immune intervention" July 17th-18th, Erlangen, Germany

Strong and sustained effector function of memory- versus naïve-derived T cells upon T-cell receptor RNA transfer: implications for cellular therapy

T Cell Telomere Length in HIV-1 Infection: No Evidence for Increased CD4+ T Cell Turnover

T cell re-targeting to EBV antigens following TCR gene transfer: CD28-containing receptors mediate enhanced antigen-specific IFNgamma production

T-cell receptor transfer for boosting HIV-1-specific T-cell immunity in HIV-1-infected patients.

Targeting of DEC-205 on human dendritic cells results in efficient MHC class II-restricted antigen presentation

The Landscape of CAR-T Cell Clinical Trials against Solid Tumors-A Comprehensive Overview

Transcriptional Targeting of Mature Dendritic Cells with Adenoviral Vectors via a Modular Promoter System for Antigen Expression and Functional Manipulation

Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells

Transfer of mRNA encoding recombinant immunoreceptors reprograms CD4+ and CD8+ T cells for use in the adoptive immunotherapy of cancer.

Triggering of NF-κB in cytokine-matured human DCs generates superior DCs for T-cell priming in cancer immunotherapy

Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells.