List of works - Toshiya Muranaka

A Dioxygenase Catalyzes Steroid 16α-Hydroxylation in Steroidal Glycoalkaloid Biosynthesis.

scientific article published on 28 July 2017

A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis

scientific article published on 16 November 2020

A new insight into application for barley chromosome addition lines of common wheat: achievement of stigmasterol accumulation

scientific article

A novel glucosyltransferase involved in steroid saponin biosynthesis in Solanum aculeatissimum

scientific article published in January 2005

A novel orfB-related gene of carrot mitochondrial genomes that is associated with homeotic cytoplasmic male sterility (CMS).

scientific article published in May 2001

AKIN10, a representative Arabidopsis SNF1-related protein kinase 1 (SnRK1), phosphorylates and downregulates plant HMG-CoA reductase

scientific article

Agrobacterium-mediated transformation of Euphorbia tirucalli callus.

scientific article published in January 2010

Ajuga Δ24-Sterol Reductase Catalyzes the Direct Reductive Conversion of 24-Methylenecholesterol to Campesterol.

scientific article published on 12 February 2016

Allylic Hydroxylation Activity Is a Source of Saponin Chemodiversity in the Genus <i>Glycyrrhiza</i>

scientific article published in 2021

Amyloplast formation in cultured tobacco BY-2 cells requires a high cytokinin content.

scientific article

Artemisinin-based antimalarial research: application of biotechnology to the production of artemisinin, its mode of action, and the mechanism of resistance of Plasmodium parasites

scientific article

Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna

scientific article published on 15 February 2019

CYP716A subfamily members are multifunctional oxidases in triterpenoid biosynthesis

scientific article

CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis

scientific article published on 22 December 2016

Characterization and engineering of glycosyltransferases responsible for steroid saponin biosynthesis in Solanaceous plants

scientific article published on 3 January 2007

Chemical phenotypes of the hmg1 and hmg2 mutants of Arabidopsis demonstrate the in-planta role of HMG-CoA reductase in triterpene biosynthesis

scientific article published in October 2007

Cloning and characterization of a squalene synthase gene from a petroleum plant, Euphorbia tirucalli L.

scientific article published on 13 March 2009

Combinatorial Biosynthesis of Legume Natural and Rare Triterpenoids in Engineered Yeast

scientific article published on 31 January 2013

Comparative analysis of CYP716A subfamily enzymes for the heterologous production of C-28 oxidized triterpenoids in transgenic yeast

scientific article published on 01 June 2018

Comparative functional analysis of CYP71AV1 natural variants reveals an important residue for the successive oxidation of amorpha-4,11-diene

article

Complete blockage of the mevalonate pathway results in male gametophyte lethality

scientific article published on 10 April 2009

Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system

article

Cytochrome P450 Monooxygenase CYP716A141 is a Unique β-Amyrin C-16β Oxidase Involved in Triterpenoid Saponin Biosynthesis in Platycodon grandiflorus ⬇️

scientific article published on 23 March 2017

DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in "drug-type" and "fiber-type" Cannabis sativa L.

scientific article

Dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols

scientific article published on 23 December 2017

Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A.

scientific article

Development of Capsicum EST-SSR markers for species identification and in silico mapping onto the tomato genome sequence.

scientific article published on 11 August 2012

Dolichol biosynthesis and its effects on the unfolded protein response and abiotic stress resistance in Arabidopsis.

scientific article

Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume

scientific article published on 24 October 2016

Dual biosynthetic pathways to phytosterol via cycloartenol and lanosterol in Arabidopsis

scholarly article

Enhanced secretory activity of Atropa belladonna hairy root culture over-expressing ADP-ribosylation factor gene

scientific article published in July 2008

Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

scientific article published in Scientific Reports

Evidence that the Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-CoA reductase 1 is phosphorylated at Ser577 in planta

scientific article published on 30 March 2018

Functional Analysis of Amorpha-4,11-Diene Synthase (ADS) Homologs from Non-Artemisinin-Producing Artemisia Species: The Discovery of Novel Koidzumiol and (+)-α-Bisabolol Synthases

scientific article published on 7 June 2016

Functional Characterization of CYP716 Family P450 Enzymes in Triterpenoid Biosynthesis in Tomato.

scientific article published on 30 January 2017

Functional characterization of CYP71D443, a cytochrome P450 catalyzing C-22 hydroxylation in the 20-hydroxyecdysone biosynthesis of Ajuga hairy roots.

scientific article

Functional specialization of UDP-glycosyltransferase 73P12 in licorice to produce a sweet triterpenoid saponin, glycyrrhizin

scientific article published on 26 June 2019

Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme

scientific article published on 01 July 1997

Generation of α-solanine-free hairy roots of potato by CRISPR/Cas9 mediated genome editing of the St16DOX gene

scientific article published on 24 April 2018

Genetic and functional characterization of Sg-4 glycosyltransferase involved in the formation of sugar chain structure at the C-3 position of soybean saponins

scientific article published on 24 September 2018

Glycyrrhiza uralensis transcriptome landscape and study of phytochemicals

scientific article

Green leaf volatiles enhance methyl jasmonate response in Arabidopsis

scientific article published on 12 July 2012

Growth and steroidal saponin production in hairy root cultures of Solanum aculeatissimum.

scientific article

Hairy root-activation tagging: a high-throughput system for activation tagging in transformed hairy roots

scientific article published in November 2005

Heterologous expression of triterpene biosynthetic genes in yeast and subsequent metabolite identification through GC-MS.

scientific article published in January 2014

Identification and characterization of (+)-α-bisabolol and 7-epi-silphiperfol-5-ene synthases from Artemisia abrotanum

scientific article published on 28 May 2019

Identification and characterization of a novel sesquiterpene synthase, 4-amorphen-11-ol synthase, from Artemisia maritima

scientific article published on 01 June 2018

Identification and genome organization of saponin pathway genes from a wild crucifer, and their use for transient production of saponins in Nicotiana benthamiana

scientific article published in September 2015

Identification of marneral synthase, which is critical for growth and development in Arabidopsis

scientific article

Identification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering preponderant α-amyrin-producing activity

scientific article published on 04 August 2019

Isolation and Characterization of the Soybean Sg-3 Gene that is Involved in Genetic Variation in Sugar Chain Composition at the C-3 Position in Soyasaponins

scientific article published on 01 April 2018

Isolation and identification of a novel chlorophenol from a cell suspension culture of Helichrysum aureonitens

scientific article published on November 2009

Lanosterol synthase in dicotyledonous plants

scientific article published on 10 March 2006

Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin

scientific article

Loss of function of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1) in Arabidopsis leads to dwarfing, early senescence and male sterility, and reduced sterol levels

scientific article published in March 2004

Lovastatin insensitive 1, a Novel pentatricopeptide repeat protein, is a potential regulatory factor of isoprenoid biosynthesis in Arabidopsis

scientific article published on 08 January 2007

Mevalonic acid partially restores chloroplast and etioplast development in Arabidopsis lacking the non-mevalonate pathway

scientific article

Novel triterpene oxidizing activity of Arabidopsis thaliana CYP716A subfamily enzymes

scientific article published on 23 January 2016

Organization and characterization of the virCD genes from Agrobacterium rhizogenes

scientific article published on 01 August 1988

P450s and UGTs: Key Players in the Structural Diversity of Triterpenoid Saponins

scientific article published on 06 May 2015

Phosphoproteome exploration reveals a reformatting of cellular processes in response to low sterol biosynthetic capacity in Arabidopsis.

scientific article published on 11 January 2012

Photobiocatalyzed asymmetric reduction of ketones using Chlorella sp. MK201

scientific article published on 25 July 2012

Phytochemical Genomics on the Way ⬇️

scientific article published on 01 May 2013

Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies ⬇️

scientific article published on 01 January 2011

Plant-derived isoprenoid sweeteners: recent progress in biosynthetic gene discovery and perspectives on microbial production.

scientific article published in December 2017

Plants utilize isoprene emission as a thermotolerance mechanism.

scientific article

Platform for "Chemical Metabolic Switching" to Increase Sesquiterpene Content in Plants

scientific article published on 23 March 2017

Production of the bioactive plant-derived triterpenoid morolic acid in engineered Saccharomyces cerevisiae

scientific article published on 20 April 2020

Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases

scientific article

Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato

scientific article published on 12 September 2014

Structure and hemolytic activity relationships of triterpenoid saponins and sapogenins.

scientific article

Structure-Activity Relationships of Pentacyclic Triterpenoids as Inhibitors of Cyclooxygenase and Lipoxygenase Enzymes

scientific article published on 27 November 2019

Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic Arabidopsis thaliana.

scientific article published on 24 July 2014

The Basic Helix-Loop-Helix Transcription Factor GubHLH3 Positively Regulates Soyasaponin Biosynthetic Genes in Glycyrrhiza uralensis

scientific article published on 01 April 2018

The NtAMI1 gene functions in cell division of tobacco BY-2 cells in the presence of indole-3-acetamide.

scientific article

The aux1 gene of the Ri plasmid is sufficient to confer auxin autotrophy in tobacco BY-2 cells.

scientific article

The mitochondrial PPR protein LOVASTATIN INSENSITIVE 1 plays regulatory roles in cytosolic and plastidial isoprenoid biosynthesis through RNA editing

scientific article published on 19 November 2009

The molecular cloning of dihydroartemisinic aldehyde reductase and its implication in artemisinin biosynthesis in Artemisia annua

scientific article published on 19 May 2010

The role of Arabidopsis ABCG9 and ABCG31 ATP binding cassette transporters in pollen fitness and the deposition of steryl glycosides on the pollen coat.

scientific article

Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin

scientific article published on 29 November 2011

Two Cytochrome P450 Monooxygenases Catalyze Early Hydroxylation Steps in the Potato Steroid Glycoalkaloid Biosynthetic Pathway.

scientific article

Upregulation of phytosterol and triterpene biosynthesis in Centella asiatica hairy roots overexpressed ginseng farnesyl diphosphate synthase

scientific article published on 27 February 2010

β-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors

scientific article published on 01 January 2012

List of works by Toshiya Muranaka

A Dioxygenase Catalyzes Steroid 16α-Hydroxylation in Steroidal Glycoalkaloid Biosynthesis.

A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis

A new insight into application for barley chromosome addition lines of common wheat: achievement of stigmasterol accumulation

A novel glucosyltransferase involved in steroid saponin biosynthesis in Solanum aculeatissimum

A novel orfB-related gene of carrot mitochondrial genomes that is associated with homeotic cytoplasmic male sterility (CMS).

AKIN10, a representative Arabidopsis SNF1-related protein kinase 1 (SnRK1), phosphorylates and downregulates plant HMG-CoA reductase

Agrobacterium-mediated transformation of Euphorbia tirucalli callus.

Ajuga Δ24-Sterol Reductase Catalyzes the Direct Reductive Conversion of 24-Methylenecholesterol to Campesterol.

Allylic Hydroxylation Activity Is a Source of Saponin Chemodiversity in the Genus <i>Glycyrrhiza</i>

Amyloplast formation in cultured tobacco BY-2 cells requires a high cytokinin content.

Artemisinin-based antimalarial research: application of biotechnology to the production of artemisinin, its mode of action, and the mechanism of resistance of Plasmodium parasites

Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna

CYP716A subfamily members are multifunctional oxidases in triterpenoid biosynthesis

CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis

Characterization and engineering of glycosyltransferases responsible for steroid saponin biosynthesis in Solanaceous plants

Chemical phenotypes of the hmg1 and hmg2 mutants of Arabidopsis demonstrate the in-planta role of HMG-CoA reductase in triterpene biosynthesis

Cloning and characterization of a squalene synthase gene from a petroleum plant, Euphorbia tirucalli L.

Combinatorial Biosynthesis of Legume Natural and Rare Triterpenoids in Engineered Yeast

Comparative analysis of CYP716A subfamily enzymes for the heterologous production of C-28 oxidized triterpenoids in transgenic yeast

Comparative functional analysis of CYP71AV1 natural variants reveals an important residue for the successive oxidation of amorpha-4,11-diene

Complete blockage of the mevalonate pathway results in male gametophyte lethality

Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system

Cytochrome P450 Monooxygenase CYP716A141 is a Unique β-Amyrin C-16β Oxidase Involved in Triterpenoid Saponin Biosynthesis in Platycodon grandiflorus ⬇️

DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in "drug-type" and "fiber-type" Cannabis sativa L.

Dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols

Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A.

Development of Capsicum EST-SSR markers for species identification and in silico mapping onto the tomato genome sequence.

Dolichol biosynthesis and its effects on the unfolded protein response and abiotic stress resistance in Arabidopsis.

Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume

Dual biosynthetic pathways to phytosterol via cycloartenol and lanosterol in Arabidopsis

Enhanced secretory activity of Atropa belladonna hairy root culture over-expressing ADP-ribosylation factor gene

Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

Evidence that the Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-CoA reductase 1 is phosphorylated at Ser577 in planta

Functional Analysis of Amorpha-4,11-Diene Synthase (ADS) Homologs from Non-Artemisinin-Producing Artemisia Species: The Discovery of Novel Koidzumiol and (+)-α-Bisabolol Synthases

Functional Characterization of CYP716 Family P450 Enzymes in Triterpenoid Biosynthesis in Tomato.

Functional characterization of CYP71D443, a cytochrome P450 catalyzing C-22 hydroxylation in the 20-hydroxyecdysone biosynthesis of Ajuga hairy roots.

Functional specialization of UDP-glycosyltransferase 73P12 in licorice to produce a sweet triterpenoid saponin, glycyrrhizin

Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme

Generation of α-solanine-free hairy roots of potato by CRISPR/Cas9 mediated genome editing of the St16DOX gene

Genetic and functional characterization of Sg-4 glycosyltransferase involved in the formation of sugar chain structure at the C-3 position of soybean saponins

Glycyrrhiza uralensis transcriptome landscape and study of phytochemicals

Green leaf volatiles enhance methyl jasmonate response in Arabidopsis

Growth and steroidal saponin production in hairy root cultures of Solanum aculeatissimum.

Hairy root-activation tagging: a high-throughput system for activation tagging in transformed hairy roots

Heterologous expression of triterpene biosynthetic genes in yeast and subsequent metabolite identification through GC-MS.

Identification and characterization of (+)-α-bisabolol and 7-epi-silphiperfol-5-ene synthases from Artemisia abrotanum

Identification and characterization of a novel sesquiterpene synthase, 4-amorphen-11-ol synthase, from Artemisia maritima

Identification and genome organization of saponin pathway genes from a wild crucifer, and their use for transient production of saponins in Nicotiana benthamiana

Identification of marneral synthase, which is critical for growth and development in Arabidopsis

Identification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering preponderant α-amyrin-producing activity

Isolation and Characterization of the Soybean Sg-3 Gene that is Involved in Genetic Variation in Sugar Chain Composition at the C-3 Position in Soyasaponins

Isolation and identification of a novel chlorophenol from a cell suspension culture of Helichrysum aureonitens

Lanosterol synthase in dicotyledonous plants

Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin

Loss of function of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1) in Arabidopsis leads to dwarfing, early senescence and male sterility, and reduced sterol levels

Lovastatin insensitive 1, a Novel pentatricopeptide repeat protein, is a potential regulatory factor of isoprenoid biosynthesis in Arabidopsis

Mevalonic acid partially restores chloroplast and etioplast development in Arabidopsis lacking the non-mevalonate pathway

Novel triterpene oxidizing activity of Arabidopsis thaliana CYP716A subfamily enzymes

Organization and characterization of the virCD genes from Agrobacterium rhizogenes

P450s and UGTs: Key Players in the Structural Diversity of Triterpenoid Saponins

Phosphoproteome exploration reveals a reformatting of cellular processes in response to low sterol biosynthetic capacity in Arabidopsis.

Photobiocatalyzed asymmetric reduction of ketones using Chlorella sp. MK201

Phytochemical Genomics on the Way ⬇️

Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies ⬇️

Plant-derived isoprenoid sweeteners: recent progress in biosynthetic gene discovery and perspectives on microbial production.

Plants utilize isoprene emission as a thermotolerance mechanism.

Platform for "Chemical Metabolic Switching" to Increase Sesquiterpene Content in Plants

Production of the bioactive plant-derived triterpenoid morolic acid in engineered Saccharomyces cerevisiae

Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases

Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato

Structure and hemolytic activity relationships of triterpenoid saponins and sapogenins.

Structure-Activity Relationships of Pentacyclic Triterpenoids as Inhibitors of Cyclooxygenase and Lipoxygenase Enzymes

Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic Arabidopsis thaliana.

The Basic Helix-Loop-Helix Transcription Factor GubHLH3 Positively Regulates Soyasaponin Biosynthetic Genes in Glycyrrhiza uralensis

The NtAMI1 gene functions in cell division of tobacco BY-2 cells in the presence of indole-3-acetamide.

The aux1 gene of the Ri plasmid is sufficient to confer auxin autotrophy in tobacco BY-2 cells.

The mitochondrial PPR protein LOVASTATIN INSENSITIVE 1 plays regulatory roles in cytosolic and plastidial isoprenoid biosynthesis through RNA editing

The molecular cloning of dihydroartemisinic aldehyde reductase and its implication in artemisinin biosynthesis in Artemisia annua

The role of Arabidopsis ABCG9 and ABCG31 ATP binding cassette transporters in pollen fitness and the deposition of steryl glycosides on the pollen coat.