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List of works by Martin R Webb

17 The stereochemical course of nucleoside triphosphatase reactions

scientific article published on 01 January 1982

A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase.

scientific article

A Secondary beta Deuterium Kinetic Isotope Effect in the Chorismate Synthase Reaction

scientific article (publication date: August 2000)

A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases

scientific article published on 01 January 2011

A biosensor for inorganic phosphate using a rhodamine-labeled phosphate binding protein

scientific article published on 01 December 2006

A continuous enzyme-coupled assay for triphosphohydrolase activity of HIV-1 restriction factor SAMHD1

scientific article

A fluorescent sensor of the phosphorylation state of nucleoside diphosphate kinase and its use to monitor nucleoside diphosphate concentrations in real time

scientific article published in April 2001

A fluorescent, reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM.

scientific article

A kinetic analysis of the nucleotide-induced allosteric transitions of GroEL.

scientific article published on October 1999

A method for determining the positional isotope exchange in a nucleoside triphosphate: cyclization of nucleoside triphosphate by dicyclohexylcarbodiimide.

scientific article published on October 1980

A new method for the time-resolved measurement of phosphate release in permeabilized muscle fibers

scientific article

A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin

scientific article published in November 2004

A tale of the unexpected

scientific article published on 01 March 2006

ATPase and shortening rates in frog fast skeletal myofibrils by time-resolved measurements of protein-bound and free Pi.

scientific article

ATPase cycle and DNA unwinding kinetics of RecG helicase

scientific article

Absence of a phosphorylated intermediate during ATP hydrolysis by Escherichia coli transcription termination protein rho.

scientific article

Activation of NADPH oxidase involves the dissociation of p21rac from its inhibitory GDP/GTP exchange protein (rhoGDI) followed by its translocation to the plasma membrane.

scientific article published on March 1994

Analysis of chiral inorganic [16O, 17O, 18O]thiophosphate and the stereochemistry of the 3-phosphoglycerate kinase reaction

scientific article published on 01 March 1980

Analysis of the ATPase mechanism of myosin subfragment 1 from insect fibrillar flight muscle in the presence and absence of actin, using phosphate-water oxygen exchange measurements.

scientific article published in December 1987

Bipolar DNA translocation contributes to highly processive DNA unwinding by RecBCD enzyme

scientific article

Changes in the ATPase activity of insect fibrillar flight muscle during calcium and strain activation probed by phosphate-water oxygen exchange.

scientific article published in June 1987

Changes in the ATPase activity of insect fibrillar flight muscle during sinusoidal length oscillation probed by phosphate-water oxygen exchange.

scientific article published in April 1988

Characterization of the GTPase reaction of elongation factor Tu. Determination of the stereochemical course in the presence of antibiotic X5108 ⬇️

scientific article published on May 10, 1982

Cross-bridge kinetics in asynchronous insect flight muscle.

scientific article published in January 1988

Crossbridge cycle in smooth muscle: kinetics assessed with flash photolysis and fluorescent probes.

scientific article

Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate

scientific article

Deconvoluting AMP-activated protein kinase (AMPK) adenine nucleotide binding and sensing

scientific article published on 14 June 2017

Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: measurement of step size and translocation speed

scientific article

Detecting intramolecular conformational dynamics of single molecules in short distance range with subnanometer sensitivity

scientific article published on 27 October 2011

Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range

scientific article published on 22 July 2015

Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase

scientific article

Direct measurement of single-stranded DNA translocation by PcrA helicase using the fluorescent base analogue 2-aminopurine

scientific article

EPR studies of the Mn(II) complex with elongation factor Tu and GDP Identification of oxygen ligands to Mn(II) by observation of 17O superhyperfine coupling

scientific article published on 01 November 1981

Electron paramagnetic resonance studies of MN(II) complexes with myosin subfragment 1 and oxygen 17-labeled ligands

scientific article published on 01 March 1982

Evidence for a novel, strongly bound acto-S1 complex carrying ADP and phosphate stabilized in the G680V mutant of Dictyostelium myosin II.

scientific article

Evidence that catalysis by yeast inorganic pyrophosphatase proceeds by direct phosphoryl transfer to water and not via a phosphoryl enzyme intermediate ⬇️

scientific article published on February 28, 1984

Evidence that phosphate release is the rate-limiting step on the overall ATPase of psoas myofibrils prevented from shortening by chemical cross-linking.

scientific article published in November 2002

Fluorescence detection of GDP in real time with the reagentless biosensor rhodamine-ParM.

scientific article

Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force

scientific article

Fluorescence lifetime imaging to detect actomyosin states in mammalian muscle sarcomeres.

scientific article

Fluorescence tools to measure helicase activity in real time.

scientific article published on 16 February 2010

Fluorescent SSB as a reagentless biosensor for single-stranded DNA.

scientific article published in January 2012

Fluorescent biosensors to investigate helicase activity

scientific article published in January 2010

Fluorescent biosensors: design and application to motor proteins

scientific article published on January 2014

Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin

scientific article

Fluorescent nucleoside triphosphates for single-molecule enzymology

scientific article published on 01 January 2011

Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity

scientific article published on 3 July 2008

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA.

scientific article published on 21 February 2018

Helicase unwinding: active or merely perfect?

scientific article

Hydrolysis of GTP by p21NRAS, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: use of a single fluorescent probe at the catalytic site

scientific article

Interaction of GTPase activating proteins (GAPs) with p21ras measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on p21ras.

scientific article published in May 1993

Kinetic analysis of the hydrolysis of GTP by p21N-ras. The basal GTPase mechanism.

scientific article

Kinetic mechanism of initiation by RepD as a part of asymmetric, rolling circle plasmid unwinding.

scientific article published on 18 April 2012

Kinetic mechanism of myosinV-S1 using a new fluorescent ATP analogue

scientific article

Kinetics of ADP dissociation from the trail and lead heads of actomyosin V following the power stroke

scientific article

Kinetics of ATP release and Pi binding during the ATPase cycle of lethocerus flight muscle fibres, using phosphate-water oxygen exchange.

scientific article published in June 1991

Kinetics of Nucleoside Triphosphate Cleavage and Phosphate Release Steps by Associated Rabbit Skeletal Actomyosin, Measured Using a Novel Fluorescent Probe for Phosphate †

scientific article (publication date: September 1997)

Kinetics of inorganic phosphate release during the interaction of p21ras with the GTPase-activating proteins, p120-GAP and neurofibromin.

scientific article published in November 1995

Kinetics of oxygen-18 exchange between inorganic phosphate and water catalyzed by myosin subfragment 1, using the 18O shift in 31P NMR ⬇️

scientific article published on May 10, 1978

Ligand-dependent dynamics of the active-site lid in bacterial dimethylarginine dimethylaminohydrolase.

scientific article published on 07 February 2014

Measurement of the reversibility of ATP binding to myosin in calcium-activated skinned fibers from rabbit skeletal muscle. Oxygen exchange between water and ATP released to the solution

scientific article published on 01 May 1989

Mechanism of GTP hydrolysis by p21N-ras catalyzed by GAP: studies with a fluorescent GTP analogue

scientific article published on 01 July 1993

Mechanism of adenosine 5'-triphosphate cleavage by myosin: studies with oxygen-18-labeled adenosine 5'-triphosphate

scientific article published on 01 October 1980

Mechanism of inorganic phosphate interaction with phosphate binding protein from Escherichia coli.

scientific article published on July 1998

Mechanism of interaction between single-stranded DNA binding protein and DNA

scientific article

Mechanism of translocation and kinetics of DNA unwinding by the helicase RecG.

scientific article

Mechanistic basis of 5'-3' translocation in SF1B helicases

scientific article

Millisecond-scale biochemical response to change in strain

scientific article published on 15 November 2011

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD.

scientific article

Mycobacterial ABC transport system: structure of the primary phosphate receptor

scientific article published on 01 July 2003

Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.

scientific article

Oxygen exchange between Pi in the medium and water during ATP hydrolysis mediated by skinned fibers from rabbit skeletal muscle. Evidence for Pi binding to a force-generating state.

scientific article published in November 1986

Oxygen exchange between phosphate and water accompanies calcium-regulated ATPase activity of skinned fibers from rabbit skeletal muscle

scientific article published on 01 March 1985

Pathway of processive ATP hydrolysis by kinesin.

scientific article published on February 1995

Phosphate release during microtubule assembly: what stabilizes growing microtubules?

scientific article

Phosphorus-31 nuclear magnetic resonance of dihydroxyacetone phosphate in the presence of triosephosphate isomerase. The question of nonproductive binding of the substrate hydrate.

scientific article published in June 1977

Pre-Steady-State Kinetics of the Microtubule • Kinesin ATPase

Probing nucleotide dissociation from myosin in vitro using microgram quantities of myosin.

scientific article published in January 2003

Purine nucleoside phosphorylase: its use in a spectroscopic assay for inorganic phosphate and for removing inorganic phosphate with the aid of phosphodeoxyribomutase

scientific article published on 01 December 1998

Raman difference studies of GDP and GTP binding to c-Harvey ras.

scientific article published in August 1998

Real-time visual detection of Pi released by flagellar dynein ATPase

scientific article

Role of magnesium in nucleotide exchange on the small G protein rac investigated using novel fluorescent Guanine nucleotide analogues.

scientific article published in March 2002

Smooth muscle myosin: regulation and properties.

scientific article

Structural prediction of a rhodamine-based biosensor and comparison with biophysical data.

scientific article

Switch 1 mutation S217A converts myosin V into a low duty ratio motor

scientific article

The ATPase cycle of PcrA helicase and its coupling to translocation on DNA.

scientific article

The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain

scientific article

The crystal structure of human rac1, a member of the rho-family complexed with a GTP analogue

scientific article

The efficiency of contraction in rabbit skeletal muscle fibres, determined from the rate of release of inorganic phosphate

scientific article published on 01 June 1999

The existence of an electrophilic component in the reaction catalysed by triose phosphate isomerase.

scientific article published on August 1974

The hydrolysis of ATP that accompanies actin polymerization is essentially irreversible

scientific article published on 01 August 1988

The interaction between rac1 and its guanine nucleotide dissociation inhibitor (GDI), monitored by a single fluorescent coumarin attached to GDI

scientific article (publication date: 25 May 1999)

The mechanism of ATP hydrolysis catalyzed by myosin and actomyosin, using rapid reaction techniques to study oxygen exchange

scientific article published on 01 November 1981

The mechanism of Ras GTPase activation by neurofibromin.

scientific article published in April 2003

The mechanism of guanosine nucleotide hydrolysis by p21 c-Ha-ras. The stereochemical course of the GTPase reaction

scientific article published on 01 April 1989

The mechanism of smooth muscle caldesmon-tropomyosin inhibition of the elementary steps of the actomyosin ATPase

scientific article published on 15 March 2006

The orientation and accessibility of substrates on the active site of triosephosphate isomerase

scientific article published on 01 October 1975

The role of nucleoside triphosphate hydrolysis in transducing systems: p21ras and muscle

scientific article published on April 29, 1992

The stereochemical course of phospho transfer catalyzed by adenylosuccinate synthetase. A reaction pathway via a phosphorylated intermediate with net inversion

scientific article published on 01 March 1984

The stereochemical course of phosphoric residue transfer catalyzed by beef heart mitochondrial ATPase.

scientific article published on December 1980

The stereochemical course of phosphoric residue transfer catalyzed by sarcoplasmic reticulum ATPase ⬇️

scientific article published on May 25, 1981

The stereochemical course of phosphoric residue transfer during the myosin ATPase reaction.

scientific article published on September 1980

The stereochemical course of the ribosome-dependent GTPase reaction of elongation factor G from Escherichia coli ⬇️

scientific article published on August 10, 1981

Time resolved measurements show that phosphate release is the rate limiting step on myofibrillar ATPases.

scientific article

Time-resolved measurements of phosphate release by cycling cross-bridges in portal vein smooth muscle

scientific article

Uncoupling DNA translocation and helicase activity in PcrA: direct evidence for an active mechanism.

scientific article published in July 2000

Vibrational study of phosphate modes in GDP and GTP and their interaction with magnesium in aqueous solution

scientific article published on 01 January 1998

Visualizing helicases unwinding DNA at the single molecule level

scientific article

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